HK1004150A1 - Calcium free subtilisin mutants. - Google Patents

Calcium free subtilisin mutants.

Info

Publication number
HK1004150A1
HK1004150A1 HK98103595A HK98103595A HK1004150A1 HK 1004150 A1 HK1004150 A1 HK 1004150A1 HK 98103595 A HK98103595 A HK 98103595A HK 98103595 A HK98103595 A HK 98103595A HK 1004150 A1 HK1004150 A1 HK 1004150A1
Authority
HK
Hong Kong
Prior art keywords
subtilisin mutants
calcium free
free subtilisin
mutants
mutated
Prior art date
Application number
HK98103595A
Other languages
English (en)
Inventor
Philip N Bryan
Patrick A Alexander
Susan L Strausberg
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Publication of HK1004150A1 publication Critical patent/HK1004150A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Cosmetics (AREA)
HK98103595A 1994-09-20 1998-04-28 Calcium free subtilisin mutants. HK1004150A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/309,069 US5567601A (en) 1993-06-01 1994-09-20 Subtilisin mutants lacking a primary calcium binding site
PCT/US1995/005520 WO1996009396A1 (fr) 1994-09-20 1995-04-28 Mutants de la subtilisine sans calcium

Publications (1)

Publication Number Publication Date
HK1004150A1 true HK1004150A1 (en) 1998-11-20

Family

ID=23196540

Family Applications (1)

Application Number Title Priority Date Filing Date
HK98103595A HK1004150A1 (en) 1994-09-20 1998-04-28 Calcium free subtilisin mutants.

Country Status (13)

Country Link
US (1) US5567601A (fr)
EP (1) EP0804592B1 (fr)
JP (1) JPH10506277A (fr)
KR (1) KR100364448B1 (fr)
CN (1) CN1168829C (fr)
AT (1) ATE320495T1 (fr)
AU (1) AU693552B2 (fr)
BR (1) BR9508981A (fr)
CA (1) CA2200563C (fr)
DE (1) DE69534875T2 (fr)
HK (1) HK1004150A1 (fr)
NZ (1) NZ285286A (fr)
WO (1) WO1996009396A1 (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
AU1225099A (en) * 1997-11-21 1999-06-15 Novo Nordisk A/S Protease variants and compositions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
WO1999049057A1 (fr) * 1998-03-26 1999-09-30 The Procter & Gamble Company Variants de serine-proteases ayant des deletions et des substitutions d'acides amines
KR20010034697A (ko) * 1998-03-26 2001-04-25 데이비드 엠 모이어 아미노산 치환을 갖는 세린 프로테아제 변이체
US6908757B1 (en) * 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
CA2376045A1 (fr) * 1999-07-22 2001-02-01 The Procter & Gamble Company Proteases variantes de la subtilisine par substitutions acide amine dans des regions definies de l'epitope
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CZ2002171A3 (cs) 1999-07-22 2002-06-12 The Procter & Gamble Company Proteinázový konjugát, čistící prostředek a prostředek osobní péče
CN1373802A (zh) * 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
US6541234B1 (en) 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
EP1321513A1 (fr) * 2001-12-21 2003-06-25 Direvo Biotech AG Variants de la subtilisine ayant des propriétés améliorées
MXPA04006811A (es) * 2002-01-16 2004-10-11 Genencor Int Variantes de proteasa substituidas con multiplos.
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
CN1791672A (zh) * 2003-03-21 2006-06-21 诺和酶股份有限公司 使用基于jp170三维结构的蛋白质建模修饰枯草杆菌酶
US7727756B2 (en) 2003-03-21 2010-06-01 Novozymes A/S Subtilases
EP1651751B1 (fr) * 2003-08-06 2015-11-18 University Of Maryland Biotechnology Institute Proteases du genie genetique pour purification par affinite et traitement des proteines de fusion
WO2016056913A1 (fr) * 2014-10-10 2016-04-14 Enzypep B.V. Condensation et cyclisation de fragments peptidiques à l'aide d'un variant de subtilisine présentant un meilleur rapport synthèse/hydrolyse rapport d'hydrolyse
GB201803654D0 (en) * 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
WO2019170918A1 (fr) * 2018-03-09 2019-09-12 Enzypep B.V. Synthèse chimio-enzymatique de liraglutide, de semaglutide et de glp-1

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
ATE116365T1 (de) * 1986-04-30 1995-01-15 Genencor Int Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte.
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
DE3856362T2 (de) * 1987-04-06 2000-05-18 Novo Nordisk A/S, Bagsvaerd Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
DE69034159T2 (de) * 1989-06-26 2005-02-17 Unilever N.V. Enzymatische Waschmittelzusammensetzungen
WO1991000335A1 (fr) * 1989-06-26 1991-01-10 Unilever Plc Compositions detergentes a base d'enzymes
BR9107258A (pt) * 1990-12-21 1994-02-22 Novo Nordisk As Protease mutante e processo compreendendo o tratamento de uma protease
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
PT717778E (pt) * 1992-07-17 2008-01-16 Genencor Int Serina proteases resistentes a alcalinidade elevada
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants

Also Published As

Publication number Publication date
DE69534875D1 (de) 2006-05-11
AU2433595A (en) 1996-04-09
EP0804592B1 (fr) 2006-03-15
BR9508981A (pt) 1997-12-30
KR100364448B1 (ko) 2003-01-24
CN1168829C (zh) 2004-09-29
CA2200563A1 (fr) 1996-03-28
NZ285286A (en) 1998-06-26
ATE320495T1 (de) 2006-04-15
AU693552B2 (en) 1998-07-02
DE69534875T2 (de) 2006-11-16
CA2200563C (fr) 2005-01-04
JPH10506277A (ja) 1998-06-23
MX9702038A (es) 1997-10-31
EP0804592A1 (fr) 1997-11-05
CN1166183A (zh) 1997-11-26
WO1996009396A1 (fr) 1996-03-28
US5567601A (en) 1996-10-22

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20100428