GR1001043B - Μεθοδος ακινητοποιησεως νουκλεινικου οξεος επι στερεας επιφανειας δια χρησιν εις δοκιμασιας, διασταυρωσεως (υβριδισμου) νουκλεινου οξεος. - Google Patents

Μεθοδος ακινητοποιησεως νουκλεινικου οξεος επι στερεας επιφανειας δια χρησιν εις δοκιμασιας, διασταυρωσεως (υβριδισμου) νουκλεινου οξεος.

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Publication number
GR1001043B
GR1001043B GR900100828A GR900100828A GR1001043B GR 1001043 B GR1001043 B GR 1001043B GR 900100828 A GR900100828 A GR 900100828A GR 900100828 A GR900100828 A GR 900100828A GR 1001043 B GR1001043 B GR 1001043B
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GR
Greece
Prior art keywords
nucleic acid
solid surface
acid strand
modified
modified nucleic
Prior art date
Application number
GR900100828A
Other languages
English (en)
Other versions
GR900100828A (en
Inventor
Chander Bahl
Rhonda Lang
Leo Mendoza
Original Assignee
Ortho Diagnostic Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Diagnostic Systems Inc filed Critical Ortho Diagnostic Systems Inc
Publication of GR900100828A publication Critical patent/GR900100828A/el
Publication of GR1001043B publication Critical patent/GR1001043B/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Aποκαλύπτονται μέθοδοι ακινητοποιήσεως νουκλεϊνικού οξέος επί στερεάς επιφανείας, δια χρήσιν εις δοκιμασίας διασταυρώσεως (υβριδισμού) νουκλεϊνικού οξέος. Αι μέθοδοι της εφευρέσεως περιλαμβάνουν αντίδραση κλώνου τροποποιημένου νουκλεϊνικού οξέος, ο οποίος περιλαμβάνει μεταβλητόν τμήμα και τμήμα αγκύρας (σταθερόν τμήμα), ένθα το μεταβλητόν τμήμα διαλαμβάνει αλληλουχίαν νουκελτοδιου έχουσα επιλεγμένην αλληλουχία βάσεως και το τμήμα αγκύρας περιλαμβάνει τουλάχιστον μίαν βάσιν νουκλεοτιδίου, τροποποιηθείσα δια λειτουργίαν πρωτοταγούς αμίνης ή ισοδύναμον βάσιν νουκλεοτιδίου έχουσα λειτουργίαν (χαρακτηριστική ομάδα) πρωτοταγούς αμίνης, και αντίδραση του κλώνου τροποποιημένου νουκλεϊνικού οξέος μετα ελευθέρας ομάδος αλδεϋδης της στερεάς επιφανείας παρουσία αναγωγικού παράγοντος, προς σχηματισμόν συμπλόκων (ενώσεων) του κλώνου τροποποιημένου νουκλεϊνικού οξέος και τουλάχιστον τμήματος των ελευθέρων ομάδων αλδεϋδης επί της στερεάς επιφανείας. ω
GR900100828A 1989-11-30 1990-11-28 Μεθοδος ακινητοποιησεως νουκλεινικου οξεος επι στερεας επιφανειας δια χρησιν εις δοκιμασιας, διασταυρωσεως (υβριδισμου) νουκλεινου οξεος. GR1001043B (el)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/444,031 US5215882A (en) 1989-11-30 1989-11-30 Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays

Publications (2)

Publication Number Publication Date
GR900100828A GR900100828A (en) 1992-04-17
GR1001043B true GR1001043B (el) 1993-03-31

Family

ID=23763208

Family Applications (1)

Application Number Title Priority Date Filing Date
GR900100828A GR1001043B (el) 1989-11-30 1990-11-28 Μεθοδος ακινητοποιησεως νουκλεινικου οξεος επι στερεας επιφανειας δια χρησιν εις δοκιμασιας, διασταυρωσεως (υβριδισμου) νουκλεινου οξεος.

Country Status (7)

Country Link
US (1) US5215882A (el)
EP (1) EP0435470B1 (el)
AT (1) ATE147860T1 (el)
CA (1) CA2031026A1 (el)
DE (1) DE69029717T2 (el)
GR (1) GR1001043B (el)
HK (1) HK95097A (el)

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Also Published As

Publication number Publication date
US5215882A (en) 1993-06-01
HK95097A (en) 1997-08-01
DE69029717T2 (de) 1997-06-05
GR900100828A (en) 1992-04-17
ATE147860T1 (de) 1997-02-15
CA2031026A1 (en) 1991-05-31
EP0435470B1 (en) 1997-01-15
EP0435470A1 (en) 1991-07-03
DE69029717D1 (de) 1997-02-27

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