GB2528344B - Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde - Google Patents
Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehydeInfo
- Publication number
- GB2528344B GB2528344B GB1503297.2A GB201503297A GB2528344B GB 2528344 B GB2528344 B GB 2528344B GB 201503297 A GB201503297 A GB 201503297A GB 2528344 B GB2528344 B GB 2528344B
- Authority
- GB
- United Kingdom
- Prior art keywords
- specimens
- liquid
- nucleic acid
- isolating nucleic
- containing formaldehyde
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 title 3
- 239000007788 liquid Substances 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 239000003755 preservative agent Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/537—Protease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N2001/002—Devices for supplying or distributing samples to an analysing apparatus
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1807910.3A GB2559919B (en) | 2014-02-28 | 2015-02-27 | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461946367P | 2014-02-28 | 2014-02-28 | |
US201461969052P | 2014-03-21 | 2014-03-21 | |
PCT/US2014/000064 WO2015130255A2 (en) | 2014-02-28 | 2014-03-21 | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
Publications (3)
Publication Number | Publication Date |
---|---|
GB201503297D0 GB201503297D0 (en) | 2015-04-15 |
GB2528344A GB2528344A (en) | 2016-01-20 |
GB2528344B true GB2528344B (en) | 2018-10-31 |
Family
ID=52876206
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1503297.2A Active GB2528344B (en) | 2014-02-28 | 2015-02-27 | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
GB1807910.3A Active GB2559919B (en) | 2014-02-28 | 2015-02-27 | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1807910.3A Active GB2559919B (en) | 2014-02-28 | 2015-02-27 | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
Country Status (2)
Country | Link |
---|---|
DE (2) | DE102015017080B3 (de) |
GB (2) | GB2528344B (de) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090202998A1 (en) * | 2005-12-16 | 2009-08-13 | Qiagen Gmbh | Method for the extraction of biomolecules from fixed tissues |
US20110196146A1 (en) * | 2010-01-04 | 2011-08-11 | Qiagen Gaithersburg Inc. | Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples |
EP2458015A1 (de) * | 2004-09-30 | 2012-05-30 | Epigenomics AG | Verfahren zur Bereitstellung von DNA-Fragmenten aus einer archivierten Probe |
US20130280728A1 (en) * | 2010-12-23 | 2013-10-24 | Qiagen Gmbh | Method and kit for processing wax-embedded biological samples |
Family Cites Families (38)
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US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US5750338A (en) | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
AU3539089A (en) | 1988-04-08 | 1989-11-03 | Salk Institute For Biological Studies, The | Ligase-based amplification method |
JP3276955B2 (ja) | 1988-09-30 | 2002-04-22 | ジーン−トラック・システムス | Rnaの鋳型の末端に結合したプローブ構造およびその使用方法 |
US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
GB9101691D0 (en) | 1991-01-25 | 1991-03-06 | British Petroleum Co Plc | Toughened resins and composites |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
ES2214472T3 (es) | 1991-08-02 | 2004-09-16 | Biomerieux B.V. | Cuantificacion de acidos nucleicos. |
AU673813B2 (en) | 1991-11-14 | 1996-11-28 | Qiagen Gaithersburg, Inc. | Non-radioactive hybridization assay and kit |
US5424413A (en) | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
KR100249110B1 (ko) | 1992-05-06 | 2000-04-01 | 다니엘 엘. 캐시앙 | 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트 |
EP0639985B1 (de) | 1992-09-10 | 2004-08-11 | Streck Laboratories, Inc. | Verfahren und zusammensetzung zur konservierung von antigenen und verfahren zur verwendung von durch selbiges verfahren hergestellten zytologischem material |
US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
DE69738687D1 (de) | 1996-04-12 | 2008-06-26 | Phri Properties Inc | Sonden, kits und assays |
US6060246A (en) | 1996-11-15 | 2000-05-09 | Avi Biopharma, Inc. | Reagent and method for isolation and detection of selected nucleic acid sequences |
US6534273B2 (en) | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
ATE340868T1 (de) | 1997-05-02 | 2006-10-15 | Gen Probe Inc | Zwei-schritt hybridisierung und einfang von einem polynukleotid |
DE19814873A1 (de) | 1998-04-02 | 1999-10-07 | Basf Ag | Verfahren zur Verminderung des C¶1¶-C¶2¶-Aldehyd-Gehalts aus Verbindungen mit CH¶2¶-CHR-0- oder -CH¶2¶-CH(OH)-Gruppen |
US6361945B1 (en) | 1998-07-02 | 2002-03-26 | Gen-Probe Incorporated | Molecular torches |
JP4871722B2 (ja) | 2003-07-25 | 2012-02-08 | アプライド バイオシステムズ リミテッド ライアビリティー カンパニー | 固定試料からrnaを調製するための方法および組成物 |
JP5020818B2 (ja) | 2004-07-01 | 2012-09-05 | ジェン−プローブ・インコーポレーテッド | 生物学的試料中の核酸を検出するための方法及び組成物 |
US9464310B2 (en) | 2005-02-10 | 2016-10-11 | Medical Diagnostic Laboratories, Llc | Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample |
AU2006218794A1 (en) | 2005-02-28 | 2006-09-08 | Gen-Probe Incorporated | Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe |
EP1877581B1 (de) | 2005-05-06 | 2012-03-28 | Gen-Probe Incorporated | Verfahren und produkte zum erfassen von nukleinsäurezielen |
JP2009544955A (ja) | 2006-07-28 | 2009-12-17 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 試料を処理するための装置 |
EP2069536B1 (de) | 2006-08-01 | 2014-11-05 | Gen-Probe Incorporated | Verfahren zur nichtspezifischen zielerfassung von nukleinsäuren |
DE102007013368A1 (de) | 2007-03-16 | 2008-09-18 | Merck Patent Gmbh | Verwendung einer Mischung eines Selbstbräuners mit einem Formaldehydfänger |
ES2472765T3 (es) | 2007-05-03 | 2014-07-03 | F. Hoffmann-La Roche Ag | Lisis y transcripción inversa mejoradas para la cuantificación de ARNm |
KR20100096253A (ko) | 2007-12-21 | 2010-09-01 | 바스프 에스이 | 영속적 자유-유동 에틸렌 우레아 |
CA2730761C (en) | 2008-08-01 | 2016-04-19 | F. Hoffmann-La Roche Ag | Improved lysis and reverse transcription for mrna quantification |
MX2011005174A (es) | 2008-11-17 | 2011-09-06 | Dow Global Technologies Llc | Metodos para reducir el formaldehido portado por el aire. |
DE102008055120A1 (de) | 2008-12-23 | 2010-07-01 | Qiagen Gmbh | Präparation und Amplifikation von Nukleinsäuren mittels magnetischer Partikel |
CA2773320C (en) | 2009-09-14 | 2018-02-20 | Qiagen Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
EP2529031B1 (de) | 2010-01-29 | 2014-07-09 | QIAGEN Gaithersburg, Inc. | Verfahren zur feststellung von hpv in einer probe |
WO2011104032A1 (en) | 2010-02-26 | 2011-09-01 | Qiagen Gmbh | Method for isolating rna from a rna and dna containing sample |
EP2661508B1 (de) | 2011-01-07 | 2018-09-12 | QIAGEN Gaithersburg, Inc. | Verfahren zur genotypierung und quantifizierung eines hochriskanten menschlichen papillomavirus |
-
2015
- 2015-02-27 GB GB1503297.2A patent/GB2528344B/en active Active
- 2015-02-27 DE DE102015017080.5A patent/DE102015017080B3/de active Active
- 2015-02-27 DE DE102015203606.5A patent/DE102015203606C5/de active Active
- 2015-02-27 GB GB1807910.3A patent/GB2559919B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2458015A1 (de) * | 2004-09-30 | 2012-05-30 | Epigenomics AG | Verfahren zur Bereitstellung von DNA-Fragmenten aus einer archivierten Probe |
US20090202998A1 (en) * | 2005-12-16 | 2009-08-13 | Qiagen Gmbh | Method for the extraction of biomolecules from fixed tissues |
US20110196146A1 (en) * | 2010-01-04 | 2011-08-11 | Qiagen Gaithersburg Inc. | Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples |
US20130280728A1 (en) * | 2010-12-23 | 2013-10-24 | Qiagen Gmbh | Method and kit for processing wax-embedded biological samples |
Also Published As
Publication number | Publication date |
---|---|
DE102015203606A1 (de) | 2015-09-03 |
DE102015203606C5 (de) | 2023-12-21 |
DE102015017080B3 (de) | 2024-01-04 |
GB201503297D0 (en) | 2015-04-15 |
GB2559919B (en) | 2018-11-14 |
GB2559919A (en) | 2018-08-22 |
GB2528344A (en) | 2016-01-20 |
DE102015203606B4 (de) | 2016-05-19 |
GB201807910D0 (en) | 2018-06-27 |
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