GB2465307A - Modified chondroitinase polypeptides - Google Patents

Modified chondroitinase polypeptides Download PDF

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Publication number
GB2465307A
GB2465307A GB1002589A GB201002589A GB2465307A GB 2465307 A GB2465307 A GB 2465307A GB 1002589 A GB1002589 A GB 1002589A GB 201002589 A GB201002589 A GB 201002589A GB 2465307 A GB2465307 A GB 2465307A
Authority
GB
United Kingdom
Prior art keywords
modified
modified chondroitinase
enzymes
active
chondroitinase polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1002589A
Other versions
GB201002589D0 (en
Inventor
Elizabeth Mary Muir
John Hubert Rogers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of GB201002589D0 publication Critical patent/GB201002589D0/en
Publication of GB2465307A publication Critical patent/GB2465307A/en
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/51—Lyases (4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention relates to modified forms of bacterial glycosaminoglycan (GAG) polysaccharide lyase enzymes, such as chondroitinase ABC, that possess a reduced number of active N-glycosylation motifs. These modified enzymes can be synthesised and secreted by mammalian cells in an active form and may be useful, for example, in the treatment of nervous system damage, including human spinal cord injury.
GB1002589A 2007-06-22 2008-06-23 Modified chondroitinase polypeptides Withdrawn GB2465307A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0712302.9A GB0712302D0 (en) 2007-06-22 2007-06-22 Chrondroitinase polypeptides
PCT/GB2008/002143 WO2009001058A1 (en) 2007-06-22 2008-06-23 Modified chondroitinase polypeptides

Publications (2)

Publication Number Publication Date
GB201002589D0 GB201002589D0 (en) 2010-03-31
GB2465307A true GB2465307A (en) 2010-05-19

Family

ID=38352881

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0712302.9A Ceased GB0712302D0 (en) 2007-06-22 2007-06-22 Chrondroitinase polypeptides
GB1002589A Withdrawn GB2465307A (en) 2007-06-22 2008-06-23 Modified chondroitinase polypeptides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0712302.9A Ceased GB0712302D0 (en) 2007-06-22 2007-06-22 Chrondroitinase polypeptides

Country Status (2)

Country Link
GB (2) GB0712302D0 (en)
WO (1) WO2009001058A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014171826A1 (en) * 2013-04-17 2014-10-23 Stichting Vu-Vumc Treatment of cognitive impairment in depressive disorders
CN108795917A (en) * 2018-07-18 2018-11-13 北京电子科技职业学院 A kind of circumscribed-type chondrosulphatase AC and the preparation method and application thereof
CN116790571A (en) * 2023-05-17 2023-09-22 西南大学 High-thermal-stability endo-alginic acid lyase mutant based on rational design modification and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0613949A2 (en) * 1993-02-24 1994-09-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
US20040091472A1 (en) * 2002-06-03 2004-05-13 Kevin Pojasek Rationally designed polysaccharide lyases derived from chrondroitinase B
WO2005087920A2 (en) * 2004-03-10 2005-09-22 Massachusetts Institute Of Technology Recombinant chondroitinase abc i and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0613949A2 (en) * 1993-02-24 1994-09-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
US20060154337A1 (en) * 1993-02-24 2006-07-13 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
US20040091472A1 (en) * 2002-06-03 2004-05-13 Kevin Pojasek Rationally designed polysaccharide lyases derived from chrondroitinase B
WO2005087920A2 (en) * 2004-03-10 2005-09-22 Massachusetts Institute Of Technology Recombinant chondroitinase abc i and uses thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J. Mol. Biol., Vol.288, 1999, Fethiere, J. et al., "Crystal structure of chondroitin...", pp.635-647 *
J. Mol. Biol., Vol.294, 1999, Huang, W. et al., "Crystal structure of chondroitinase B...", pp.1257-1269 *

Also Published As

Publication number Publication date
GB0712302D0 (en) 2007-08-01
WO2009001058A1 (en) 2008-12-31
GB201002589D0 (en) 2010-03-31

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)