GB1377346A - Method of and reagent for testing blood - Google Patents
Method of and reagent for testing bloodInfo
- Publication number
- GB1377346A GB1377346A GB1444373A GB1444373A GB1377346A GB 1377346 A GB1377346 A GB 1377346A GB 1444373 A GB1444373 A GB 1444373A GB 1444373 A GB1444373 A GB 1444373A GB 1377346 A GB1377346 A GB 1377346A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- hemoglobin
- sickle
- grams
- indicates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1377346 Diagnostic agent for detecting sicklecell disease BAXTER LABORATORIES Inc 26 March 1973 [24 March 1972] 14443/73 Heading G1B A reagent kit for use in testing blood for sicklecell disease and for distinguishing between sicklecell anemia and sickle-cell trait comprises (a) an aqueous buffer solution having a pH of from 7 to 7À5 and containing, per 100 ml of solution, from 21 to 35 grams of (NH 4 ) 2 SO 4 and from 0À5 to 2À0 grams of K 2 HPO 4 , (b) a hemolyzing agent, for example saponin, and (c) a reducing agent, for example sodium dithionite. The hemolyzing agent and the reducing agent may be in powdered form. Reagent solution prepared by mixing the contents of such kit may contain, per 100 ml of solution, from 0À01 to 2 grams of hemolyzing agent and from 0À01 to 2 grams of reducing agent. A sample of unclotted blood is mixed with a predetermined amount of reagent solution, the mixture being observed visually both before and after centrifuging. A translucent solution before centrifuging indicates normal (AA) hemoglobin whereas a turbid solution indicates either sickle-cell trait (AS) hemoglobin or sickle-cell anemia (SS) hemoglobin; a small amount of white precipitate after centrifuging indicates normal hemoglobin whereas a pink solution with red precipitate indicates sickle-cell hemoglobin and a yellow solution with red precipitate indicates sickle-cell anemia hemoglobin. The buffer solution additionally may contain a redox dye, for example methylene blue or basic fuchsin, for indicating decomposition of sodium dithionite reducing agent.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US23792472A | 1972-03-24 | 1972-03-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1377346A true GB1377346A (en) | 1974-12-11 |
Family
ID=22895800
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1444373A Expired GB1377346A (en) | 1972-03-24 | 1973-03-26 | Method of and reagent for testing blood |
Country Status (4)
Country | Link |
---|---|
US (1) | US3761226A (en) |
CA (1) | CA1009574A (en) |
GB (1) | GB1377346A (en) |
ZA (1) | ZA731900B (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3918905A (en) * | 1973-07-20 | 1975-11-11 | Biolog Corp Of America | Diagnostic test for the determination of sickling hemoglobinopathies |
US4236526A (en) * | 1978-01-31 | 1980-12-02 | Richard Patricia A | Method of screening for sickle cell disease by detection of porphyrins and porphyrin metabolites in human dentition |
US5514592A (en) * | 1995-02-13 | 1996-05-07 | Medicus Technologies, Inc. | Method and composition for testing blood for hemoglobin S that incorporates mineral oil as an insoluble upper phase |
US8623659B2 (en) | 2010-09-24 | 2014-01-07 | Saint Louis University | Sickle confirm modified hemoglobin solubility test |
US9448230B2 (en) | 2012-11-21 | 2016-09-20 | Saint Louis Univeristy | Method of hemoglobin-F determination |
-
1972
- 1972-03-24 US US00237924A patent/US3761226A/en not_active Expired - Lifetime
-
1973
- 1973-03-19 ZA ZA731900A patent/ZA731900B/en unknown
- 1973-03-23 CA CA166,839A patent/CA1009574A/en not_active Expired
- 1973-03-26 GB GB1444373A patent/GB1377346A/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ZA731900B (en) | 1973-12-19 |
CA1009574A (en) | 1977-05-03 |
US3761226A (en) | 1973-09-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed [section 19, patents act 1949] | ||
PCNP | Patent ceased through non-payment of renewal fee |