GB1174045A - A Fermentation Process - Google Patents

A Fermentation Process

Info

Publication number
GB1174045A
GB1174045A GB5435966A GB5435966A GB1174045A GB 1174045 A GB1174045 A GB 1174045A GB 5435966 A GB5435966 A GB 5435966A GB 5435966 A GB5435966 A GB 5435966A GB 1174045 A GB1174045 A GB 1174045A
Authority
GB
United Kingdom
Prior art keywords
penicillinamidase
water
broth
aqueous solution
quaternary ammonium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5435966A
Inventor
David Aaron Johnson
Glenn Alfred Hardcastle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Co
Original Assignee
Bristol Myers Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Co filed Critical Bristol Myers Co
Priority to GB5435966A priority Critical patent/GB1174045A/en
Publication of GB1174045A publication Critical patent/GB1174045A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1,174,045. 6-Aminopenicillanic acid. BRISTOL-MYERS CO. 5 Dec., 1966, No. 64359/66. Heading C2A. [Also in Division C3] A process for the production of a purified aqueous solution of penicillinamidase comprises the steps of: (1) adding to a penicillinamidasecontaining fermentation broth a quaternary ammonium salt and a metal hydroxide or a metal salt having a divalent metallic cation which does not destroy or inhibit penicillinamidase, whereby the penicillinamidase is caused to be affixed to the water-insoluble solids present in the broth, the amount of quaternary ammonium salt being such that the yield of penicillinamidase is greater than that obtained in the absence of the quaternary ammonium salt; (2) separating the water-insoluble solids from the broth; (3) mixing the water-insoluble solids with water, whereby said penicillinamidase is dissolved in the water; (4) separating the penicillinamidase-containing supernatant water layer from the solid materials; (5) adding to the separated water layer a solid absorbing agent incapable of absorbing penicillinamidase; and (6) removing the solid absorbent from the aqueous solution to provide a purified aqueous solution of penicillinamidase. The broth may result from the cultivation of Escherichia Coli in an appropriate nutrient medium. The penicillinamidase may be used to hydrolyse potassium benzyl pencillin to 6-aminopenicillanic acid which may then be used as the starting material for semi-synthetic penicillins.
GB5435966A 1966-12-05 1966-12-05 A Fermentation Process Expired GB1174045A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB5435966A GB1174045A (en) 1966-12-05 1966-12-05 A Fermentation Process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB5435966A GB1174045A (en) 1966-12-05 1966-12-05 A Fermentation Process

Publications (1)

Publication Number Publication Date
GB1174045A true GB1174045A (en) 1969-12-10

Family

ID=10470742

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5435966A Expired GB1174045A (en) 1966-12-05 1966-12-05 A Fermentation Process

Country Status (1)

Country Link
GB (1) GB1174045A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001036592A1 (en) 1999-11-12 2001-05-25 Mitsubishi Rayon Co., Ltd. Sterilized microbial cells

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001036592A1 (en) 1999-11-12 2001-05-25 Mitsubishi Rayon Co., Ltd. Sterilized microbial cells
EP1233057A1 (en) * 1999-11-12 2002-08-21 Mitsubishi Rayon Co., Ltd. Sterilized microbial cells
EP1233057A4 (en) * 1999-11-12 2004-11-03 Mitsubishi Rayon Co Sterilized microbial cells

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Legal Events

Date Code Title Description
PS Patent sealed
PLNP Patent lapsed through nonpayment of renewal fees