GB1007972A - Biochemical process for the preparation of cyanocobalamin - Google Patents

Biochemical process for the preparation of cyanocobalamin

Info

Publication number
GB1007972A
GB1007972A GB4430260A GB4430260A GB1007972A GB 1007972 A GB1007972 A GB 1007972A GB 4430260 A GB4430260 A GB 4430260A GB 4430260 A GB4430260 A GB 4430260A GB 1007972 A GB1007972 A GB 1007972A
Authority
GB
United Kingdom
Prior art keywords
vitamin
analogues
culture
cells
cobalamin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4430260A
Inventor
Ivan Rolovich
Giorgio Ragni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pierrel SpA
Original Assignee
Pierrel SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierrel SpA filed Critical Pierrel SpA
Priority to GB4430260A priority Critical patent/GB1007972A/en
Priority to CH1476661A priority patent/CH418527A/en
Priority to DE1961P0028467 priority patent/DE1239694B/en
Publication of GB1007972A publication Critical patent/GB1007972A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Hydroxy cobalamin and Vitamin B12 are obtained by cultivating Propionibacterium Freudenreichii or Propionibacterium Shermanii, inoculating a suitable medium with the culture, thereby producing Vitamin B12 and its analogues and then, at the end of its growth and production cycle, separating the cells from the broth, and suspending these in physiological saline in the presence of 5-6 dimethylbenzimidazole or this compound together with a non-toxic salt capable of releasing cyanide ions such as sodium thiocyanate or potassium thiocyanate so as to convert the analogues to cobalamin and Vitamin B12. In examples, the bacteria are kept in a "stub culture" prepared with peptone, meat extract, yeast, tomato juice and agar and then this culture is grown on a coin steep-dextron medium. The cells are separated as a dense cream and are then suspended in sodium chloride solution and the required precursors added. The reaction is stopped when optimum conversion of the analogues to Vitamin B12 has been effected. The vitamin may be extracted by the method described in Specification 1,007,971.
GB4430260A 1960-12-23 1960-12-23 Biochemical process for the preparation of cyanocobalamin Expired GB1007972A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GB4430260A GB1007972A (en) 1960-12-23 1960-12-23 Biochemical process for the preparation of cyanocobalamin
CH1476661A CH418527A (en) 1960-12-23 1961-12-20 Process for preparing vitamin B12
DE1961P0028467 DE1239694B (en) 1960-12-23 1961-12-22 Process for the production of vitamin B and its biologically active derivatives

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4430260A GB1007972A (en) 1960-12-23 1960-12-23 Biochemical process for the preparation of cyanocobalamin

Publications (1)

Publication Number Publication Date
GB1007972A true GB1007972A (en) 1965-10-22

Family

ID=10432664

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4430260A Expired GB1007972A (en) 1960-12-23 1960-12-23 Biochemical process for the preparation of cyanocobalamin

Country Status (3)

Country Link
CH (1) CH418527A (en)
DE (1) DE1239694B (en)
GB (1) GB1007972A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2102332A1 (en) * 1996-01-03 1997-07-16 Invest Y Desarrollo Agroindust Process for the production of vitamin B12 from polluting wastes in the citrus growing industries.
DE102013008336A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Producing brewing malt, involves providing malting raw material, preferably grain including barley or wheat, and treating raw material, preferably grain with lactic acid bacteria of strain Lactobacillus coryniformis
DE102013100891A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of an acid, a mash or wort, a drink, a concentrate and the products produced therewith and use of lactic acid bacteria, an acid, a mash, a wort or a concentrate
DE102013008335A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of a food as well as the food produced therewith and use of lactic acid bacteria, an acid, a mash, a wort, a drink or a concentrate
DE102014110182A1 (en) 2014-07-18 2016-01-21 Erdinger Weißbräu Werner Brombach GmbH & Co. KG Process for the preparation of a food or a precursor thereof, food or a precursor thereof and corresponding use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1091705B (en) * 1959-11-27 1960-10-27 Hoffmann La Roche Process for the production of vitamins of the B group by propionic acid bacteria

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2102332A1 (en) * 1996-01-03 1997-07-16 Invest Y Desarrollo Agroindust Process for the production of vitamin B12 from polluting wastes in the citrus growing industries.
DE102013008336A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Producing brewing malt, involves providing malting raw material, preferably grain including barley or wheat, and treating raw material, preferably grain with lactic acid bacteria of strain Lactobacillus coryniformis
DE102013100891A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of an acid, a mash or wort, a drink, a concentrate and the products produced therewith and use of lactic acid bacteria, an acid, a mash, a wort or a concentrate
DE102013008335A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of a food as well as the food produced therewith and use of lactic acid bacteria, an acid, a mash, a wort, a drink or a concentrate
WO2014118190A1 (en) 2013-01-29 2014-08-07 Erdinger Weissbräu Franz Brombach e.K. Method for producing an acid malt, an acid malt, method for producing a mash or wort, mash or wort, method for producing a drink, a drink, method for producing a concentrate, a concentrate
WO2014118191A1 (en) 2013-01-29 2014-08-07 Erdinger Weissbräu Franz Brombach e.K. Method for producing a foodstuff and the thus produced foodstuff and use of lactic acid bacteria, acid malt, a mash, a wort, a drink or a concentrate
DE102014110182A1 (en) 2014-07-18 2016-01-21 Erdinger Weißbräu Werner Brombach GmbH & Co. KG Process for the preparation of a food or a precursor thereof, food or a precursor thereof and corresponding use
EP2995204A1 (en) 2014-07-18 2016-03-16 Erdinger Weißbräu Werner Brombach GmbH & Co. KG Method for the preparation of a food or a precursor of the same, food or a precursor of the same, and uses of same

Also Published As

Publication number Publication date
CH418527A (en) 1966-08-15
DE1239694B (en) 1967-05-03

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