FR3092172B1 - Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP - Google Patents
Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP Download PDFInfo
- Publication number
- FR3092172B1 FR3092172B1 FR1900880A FR1900880A FR3092172B1 FR 3092172 B1 FR3092172 B1 FR 3092172B1 FR 1900880 A FR1900880 A FR 1900880A FR 1900880 A FR1900880 A FR 1900880A FR 3092172 B1 FR3092172 B1 FR 3092172B1
- Authority
- FR
- France
- Prior art keywords
- container
- protein
- molecule
- ret
- gtp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 title abstract 7
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 title abstract 7
- 230000004913 activation Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 101100122490 Drosophila melanogaster Galphaq gene Proteins 0.000 abstract 3
- 239000003446 ligand Substances 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 2
- 108091006027 G proteins Proteins 0.000 abstract 1
- 102000030782 GTP binding Human genes 0.000 abstract 1
- 108091000058 GTP-Binding Proteins 0.000 abstract 1
- 239000000556 agonist Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4719—G-proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP L’invention concerne une méthode pour déterminer la capacité d’une molécule à moduler l’activation d’un récepteur couplé à une protéine G (RCPG), ladite méthode comprenant les étapes suivantes :a) introduction, dans un premier contenant : - d’une préparation membranaire portant un ou plusieurs RCPG et une ou plusieurs protéine(s) Galpha, - d’une source de GTP non-hydrolysable ou lentement hydrolysable marqué par un premier membre d’un couple de partenaires de RET, - d’un ligand de la sous-unité alpha d’une protéine G (protéine Galpha) marqué par un second membre du couple de partenaires de RET, ledit ligand étant capable de se lier à la protéine Galpha pleine liée au GTP non-hydrolysable ou lentement hydrolysable marqué par le premier membre d’un couple de partenaires de RET, - éventuellement d’un agoniste du RCPG ; b) mesure du signal de RET émis dans le premier contenant ;c) introduction (i) dans un second contenant, des mêmes réactifs qu’à l’étape a) et de la molécule à tester ou (ii) dans le premier contenant, de la molécule à tester ;d) mesure du signal de RET émis dans le second contenant ou dans le premier contenant obtenu à l’étape c) ;e) comparaison des signaux obtenus aux étapes b) et d), une modulation du signal obtenu à l’étape d) par rapport à celui obtenu à l’étape b) indiquant que la molécule à tester est capable de moduler l’activation du RCPG.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1900880A FR3092172B1 (fr) | 2019-01-30 | 2019-01-30 | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
PCT/FR2020/050151 WO2020157441A1 (fr) | 2019-01-30 | 2020-01-30 | Methode pour mesurer la modulation de l'activation d'un recepteur couple a une proteine g avec des analogues du gtp |
CA3127858A CA3127858A1 (fr) | 2019-01-30 | 2020-01-30 | Methode pour mesurer la modulation de l'activation d'un recepteur couple a une proteine g avec des analogues du gtp |
CN202080026898.3A CN113711039A (zh) | 2019-01-30 | 2020-01-30 | 用gtp类似物测量对g蛋白偶联受体激活的调控的方法 |
US17/426,571 US20220099669A1 (en) | 2019-01-30 | 2020-01-30 | Method for measuring the modulation of the activation of a g protein-coupled receptor with gtp analogues |
JP2021544510A JP7498720B2 (ja) | 2019-01-30 | 2020-01-30 | Gtp類似体を用いてgタンパク質共役受容体の活性化の変化を測定する方法 |
EP20708542.4A EP3918328A1 (fr) | 2019-01-30 | 2020-01-30 | Methode pour mesurer la modulation de l'activation d'un recepteur couple a une proteine g avec des analogues du gtp |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1900880 | 2019-01-30 | ||
FR1900880A FR3092172B1 (fr) | 2019-01-30 | 2019-01-30 | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
Publications (2)
Publication Number | Publication Date |
---|---|
FR3092172A1 FR3092172A1 (fr) | 2020-07-31 |
FR3092172B1 true FR3092172B1 (fr) | 2021-02-12 |
Family
ID=67514722
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR1900880A Active FR3092172B1 (fr) | 2019-01-30 | 2019-01-30 | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
Country Status (7)
Country | Link |
---|---|
US (1) | US20220099669A1 (fr) |
EP (1) | EP3918328A1 (fr) |
JP (1) | JP7498720B2 (fr) |
CN (1) | CN113711039A (fr) |
CA (1) | CA3127858A1 (fr) |
FR (1) | FR3092172B1 (fr) |
WO (1) | WO2020157441A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR3069644B1 (fr) * | 2017-07-28 | 2024-07-12 | Cisbio Bioassays | Methode pour mesurer la modulation de l'activation d'un recepteur couple a une proteine g |
CN114277072B (zh) * | 2021-08-05 | 2023-10-24 | 清华大学 | 一种基于kras蛋白的核苷酸交换方法 |
Family Cites Families (33)
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US4801722A (en) | 1981-07-01 | 1989-01-31 | Eastman Kodak Company | Coumarin chelates |
US4837169A (en) | 1981-07-01 | 1989-06-06 | Eastman Kodak Company | Polypyridine Fluorescent labels for immunoassay |
US4670572A (en) | 1981-07-01 | 1987-06-02 | Eastman Kodak Company | Phenolic fluorescent labels |
US4859777A (en) | 1981-07-01 | 1989-08-22 | Eastman Kodak Company | Terpyridine chelating agents |
US4637988A (en) | 1981-07-01 | 1987-01-20 | Eastman Kodak Company | Fluorescent labels for immunoassay |
US4794191A (en) | 1981-07-01 | 1988-12-27 | Eastman Kodak Company | Fluorescent chelates |
FR2570703B1 (fr) | 1984-09-26 | 1988-07-08 | Commissariat Energie Atomique | Complexes macropolycycliques de terres rares et application a titre de marqueurs fluorescents |
US4761481A (en) | 1985-03-18 | 1988-08-02 | Baxter Travenol Laboratories, Inc. | Substituted pyridine derivatives |
US5055578A (en) | 1987-11-06 | 1991-10-08 | Baxter Diagnostics Inc. | Fluorescent poly(arylpyridine) rare earth chelates |
US5116989A (en) | 1987-11-06 | 1992-05-26 | Baxter Diagnostics Inc. | Fluorescent poly(arylpyridine) rare earth chelates |
US5032677A (en) | 1987-11-06 | 1991-07-16 | Baxter International Inc. | Fluorescent poly(arylpyridine) rare earth chelates |
US5106957A (en) | 1987-11-06 | 1992-04-21 | Baxter Diagnostics Inc. | Fluorescent poly(arylpyridine) rare earth chelates |
FR2624862B1 (fr) | 1987-12-18 | 1990-06-08 | Oris Ind | Cryptates de terres rares, procedes d'obtention, intermediaires de synthese et application a titre de marqueurs fluorescents |
US5202423A (en) | 1988-07-08 | 1993-04-13 | Wallac Oy | Terpyridine derivatives |
SE8802575D0 (sv) | 1988-07-08 | 1988-07-08 | Wallac Oy | Terpyridine derivatives |
FI88654C (fi) | 1991-03-15 | 1993-06-10 | Datacity Center Oy | Fluorescenshoejningsmetod |
FR2680787B1 (fr) | 1991-08-30 | 1994-11-04 | Cis Bio Int | Complexes macrocycliques de terres rares et leur utilisation pour reduire les interferences dans un dosage par fluorescence. |
US5622821A (en) | 1994-06-29 | 1997-04-22 | The Regents Of The University Of California | Luminescent lanthanide chelates and methods of use |
AU768957B2 (en) | 1999-02-18 | 2004-01-08 | Regents Of The University Of California, The | Phthalamide-lanthanide complexes for use as luminescent markers |
WO2000048991A1 (fr) | 1999-02-18 | 2000-08-24 | The Regents Of The University Of California | Complexes salicylamides lanthanides utilises comme marqueurs luminescents |
FR2810406B1 (fr) | 2000-06-15 | 2002-09-20 | Cis Bio Int | Nouveaux cryptates de terre rare peu sensibles a l'extinction de fluorescence |
WO2003008435A2 (fr) | 2001-07-06 | 2003-01-30 | Board Of Trustees Of The University Of Illinois | Proteines de fusion gfp et utilisation de ces proteines |
WO2004035614A1 (fr) | 2001-07-11 | 2004-04-29 | Karo Bio Ab | Peptides synthetiques ou partiellement purifies pouvant se lier a des sous-unites specifiques de proteines g, et leurs utilisations |
GB0421693D0 (en) * | 2004-09-30 | 2004-11-03 | Amersham Biosciences Uk Ltd | Method for measuring binding of a test compound to a G-protein coupled receptor |
US7445902B2 (en) * | 2004-09-30 | 2008-11-04 | Ge Healthcare Uk Limited | Fluorescent nucleotide analogues |
US9029097B2 (en) | 2005-02-16 | 2015-05-12 | Universite De Montreal | Biosensors for monitoring receptor-mediated G-protein activation |
US8173800B2 (en) | 2006-08-15 | 2012-05-08 | The Regents Of The University Of California | Luminescent macrocyclic lanthanide complexes |
GB2451106A (en) | 2007-07-18 | 2009-01-21 | Cis Bio Int | Lanthanide (III) ion complexing pyrazoyl-aza(thio)xanthone comprising compounds, their complexes and their use as fluorescent labels |
US20090131263A1 (en) | 2007-11-19 | 2009-05-21 | Longying Dong | Normalization methods for G-protein coupled receptor membrane array |
EP2425249B1 (fr) * | 2009-04-30 | 2014-07-16 | Cisbio Bioassays | Procede de detection de composes modulateurs de dimeres de proteines membranaires a domaine vft |
FR2949156B1 (fr) | 2009-08-13 | 2016-04-15 | Cis-Bio Int | Methode de determination de la liaison d'un compose donne a un recepteur membranaire |
NO2723764T3 (fr) | 2011-09-15 | 2018-05-26 | ||
PL3207052T3 (pl) | 2014-10-14 | 2021-02-08 | Université de Montréal | Bioczujnik oparty na białkach oddziałujących z betagamma w celu monitorowania aktywacji białka g |
-
2019
- 2019-01-30 FR FR1900880A patent/FR3092172B1/fr active Active
-
2020
- 2020-01-30 CA CA3127858A patent/CA3127858A1/fr active Pending
- 2020-01-30 WO PCT/FR2020/050151 patent/WO2020157441A1/fr unknown
- 2020-01-30 CN CN202080026898.3A patent/CN113711039A/zh active Pending
- 2020-01-30 US US17/426,571 patent/US20220099669A1/en active Pending
- 2020-01-30 JP JP2021544510A patent/JP7498720B2/ja active Active
- 2020-01-30 EP EP20708542.4A patent/EP3918328A1/fr active Pending
Also Published As
Publication number | Publication date |
---|---|
FR3092172A1 (fr) | 2020-07-31 |
JP2022523099A (ja) | 2022-04-21 |
EP3918328A1 (fr) | 2021-12-08 |
US20220099669A1 (en) | 2022-03-31 |
WO2020157441A1 (fr) | 2020-08-06 |
CA3127858A1 (fr) | 2020-08-06 |
JP7498720B2 (ja) | 2024-06-12 |
CN113711039A (zh) | 2021-11-26 |
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