FR2959820B1 - METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM - Google Patents

METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

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Publication number
FR2959820B1
FR2959820B1 FR1053630A FR1053630A FR2959820B1 FR 2959820 B1 FR2959820 B1 FR 2959820B1 FR 1053630 A FR1053630 A FR 1053630A FR 1053630 A FR1053630 A FR 1053630A FR 2959820 B1 FR2959820 B1 FR 2959820B1
Authority
FR
France
Prior art keywords
liquid medium
magnetic field
analyte
measuring
magnetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR1053630A
Other languages
French (fr)
Other versions
FR2959820A1 (en
Inventor
Jean Baudry
Jerome Bibette
Elodie Brient-Litzler
Eric Compain
Catherine Bonhomme
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bertin Technologies SAS
Original Assignee
Bertin Technologies SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bertin Technologies SAS filed Critical Bertin Technologies SAS
Priority to FR1053630A priority Critical patent/FR2959820B1/en
Publication of FR2959820A1 publication Critical patent/FR2959820A1/en
Application granted granted Critical
Publication of FR2959820B1 publication Critical patent/FR2959820B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

Abstract

The process for assaying an analyte in a liquid medium by reaction of analyte with ligands carried by magnetic particles, comprises measuring a magnetic field acting on the liquid medium, measuring an amplitude or variation of a parameter that is representative of an optical property and polarimetric properties of the liquid medium and the cutting of the magnetic field for measuring and deducing an analyte concentration in the liquid medium, and applying a first magnetic field having an intensity and an orientation data to the liquid medium. The process for assaying an analyte in a liquid medium by reaction of analyte with ligands carried by magnetic particles, comprises measuring a magnetic field acting on the liquid medium, measuring an amplitude or variation of a parameter that is representative of an optical property and polarimetric properties of the liquid medium and the cutting of the magnetic field for measuring and deducing an analyte concentration in the liquid medium, applying a first magnetic field having an intensity and an orientation data to the liquid medium, applying a second magnetic field having an intensity and/or perpendicular orientation to the liquid medium, measuring the variation of the parameter between two magnetic fields, placing magnetic particles of different categories in the liquid medium, placing two analytes in the liquid medium, measuring a signal due to the orientation of magnetic clusters corresponding to the first and second analytes, and deducing the concentrations of the analytes of variations of signals during analyte-ligand reactions. The analyte-ligand reaction causes the formation of magnetic particle clusters by bridging and by measuring the parameter representative of the optical property of the liquid medium. The step of measuring the parameter is carried out by the application or modification of the magnetic field for a duration of = 1 second. The concentration of the analyte is deduced from the variation of measurement. The magnetic field of reaction is followed by a relaxation period, which is followed by a measurement of the parameter by the application of the magnetic field. A duration of application of the magnetic field of reaction progressively increases. A cutoff duration of the magnetic field of reaction is constant. The categories are differentiated by the particle size and/or magnetic properties. The particles of first and second categories are functionalized with ligands specific to first and second analytes. The process further comprises verifying whether a variation of the parameter takes place due to clusters corresponding to magnetic particles of the second category during the application of magnetic field. The liquid medium contains magnetic particles of a first category functionalized with ligands specific to the analyte to be assayed, and magnetic particles of a second category-functionalized with ligands do not react with the liquid medium and the analyte to be assayed.
FR1053630A 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM Expired - Fee Related FR2959820B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR1053630A FR2959820B1 (en) 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1053630A FR2959820B1 (en) 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

Publications (2)

Publication Number Publication Date
FR2959820A1 FR2959820A1 (en) 2011-11-11
FR2959820B1 true FR2959820B1 (en) 2013-03-15

Family

ID=43217165

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1053630A Expired - Fee Related FR2959820B1 (en) 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

Country Status (1)

Country Link
FR (1) FR2959820B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3130994A1 (en) 2021-12-21 2023-06-23 Horiba Abx Sas DOSAGE PROCEDURE

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2832507B1 (en) * 2001-11-20 2004-02-20 Stago Diagnostica METHOD OF DETECTING ANALYTE (S) USING COLLOIDAL MAGNETIC PARTICLES
FR2919390B1 (en) * 2007-07-27 2009-10-30 Bertin Technologies Soc Par Ac METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

Also Published As

Publication number Publication date
FR2959820A1 (en) 2011-11-11

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