FR2959820A1 - Assaying analyte in liquid medium by reaction of analyte with ligands carried by magnetic particles, comprises measuring magnetic field acting on medium, and measuring amplitude of parameter representative of optical property of medium - Google Patents

Assaying analyte in liquid medium by reaction of analyte with ligands carried by magnetic particles, comprises measuring magnetic field acting on medium, and measuring amplitude of parameter representative of optical property of medium Download PDF

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Publication number
FR2959820A1
FR2959820A1 FR1053630A FR1053630A FR2959820A1 FR 2959820 A1 FR2959820 A1 FR 2959820A1 FR 1053630 A FR1053630 A FR 1053630A FR 1053630 A FR1053630 A FR 1053630A FR 2959820 A1 FR2959820 A1 FR 2959820A1
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France
Prior art keywords
liquid medium
analyte
magnetic field
measuring
magnetic
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FR1053630A
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French (fr)
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FR2959820B1 (en
Inventor
Jean Baudry
Jerome Bibette
Elodie Brient-Litzler
Eric Compain
Catherine Bonhomme
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Bertin Technologies SAS
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Bertin Technologies SAS
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Publication of FR2959820B1 publication Critical patent/FR2959820B1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Soft Magnetic Materials (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The process for assaying an analyte in a liquid medium by reaction of analyte with ligands carried by magnetic particles, comprises measuring a magnetic field acting on the liquid medium, measuring an amplitude or variation of a parameter that is representative of an optical property and polarimetric properties of the liquid medium and the cutting of the magnetic field for measuring and deducing an analyte concentration in the liquid medium, and applying a first magnetic field having an intensity and an orientation data to the liquid medium. The process for assaying an analyte in a liquid medium by reaction of analyte with ligands carried by magnetic particles, comprises measuring a magnetic field acting on the liquid medium, measuring an amplitude or variation of a parameter that is representative of an optical property and polarimetric properties of the liquid medium and the cutting of the magnetic field for measuring and deducing an analyte concentration in the liquid medium, applying a first magnetic field having an intensity and an orientation data to the liquid medium, applying a second magnetic field having an intensity and/or perpendicular orientation to the liquid medium, measuring the variation of the parameter between two magnetic fields, placing magnetic particles of different categories in the liquid medium, placing two analytes in the liquid medium, measuring a signal due to the orientation of magnetic clusters corresponding to the first and second analytes, and deducing the concentrations of the analytes of variations of signals during analyte-ligand reactions. The analyte-ligand reaction causes the formation of magnetic particle clusters by bridging and by measuring the parameter representative of the optical property of the liquid medium. The step of measuring the parameter is carried out by the application or modification of the magnetic field for a duration of = 1 second. The concentration of the analyte is deduced from the variation of measurement. The magnetic field of reaction is followed by a relaxation period, which is followed by a measurement of the parameter by the application of the magnetic field. A duration of application of the magnetic field of reaction progressively increases. A cutoff duration of the magnetic field of reaction is constant. The categories are differentiated by the particle size and/or magnetic properties. The particles of first and second categories are functionalized with ligands specific to first and second analytes. The process further comprises verifying whether a variation of the parameter takes place due to clusters corresponding to magnetic particles of the second category during the application of magnetic field. The liquid medium contains magnetic particles of a first category functionalized with ligands specific to the analyte to be assayed, and magnetic particles of a second category-functionalized with ligands do not react with the liquid medium and the analyte to be assayed.
FR1053630A 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM Expired - Fee Related FR2959820B1 (en)

Priority Applications (1)

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FR1053630A FR2959820B1 (en) 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

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Application Number Priority Date Filing Date Title
FR1053630A FR2959820B1 (en) 2010-05-10 2010-05-10 METHOD FOR ASSAYING AN ANALYTE IN A LIQUID MEDIUM

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FR2959820A1 true FR2959820A1 (en) 2011-11-11
FR2959820B1 FR2959820B1 (en) 2013-03-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3130994A1 (en) 2021-12-21 2023-06-23 Horiba Abx Sas DOSAGE PROCEDURE

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1446666A1 (en) * 2001-11-20 2004-08-18 Diagnostica Stago Method for detecting analyte(s) using magnetic colloidal particles
WO2009034271A1 (en) * 2007-07-27 2009-03-19 Bertin Technologies Method for dosing an analyte in a liquid medium

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1446666A1 (en) * 2001-11-20 2004-08-18 Diagnostica Stago Method for detecting analyte(s) using magnetic colloidal particles
WO2009034271A1 (en) * 2007-07-27 2009-03-19 Bertin Technologies Method for dosing an analyte in a liquid medium

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BAUDRY J ET AL: "Acceleration of the recognition rate between grafted ligands and receptors with magnetic forces.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 31 OCT 2006 LNKD- PUBMED:17050677, vol. 103, no. 44, 31 October 2006 (2006-10-31), pages 16076 - 16078, XP002616464, ISSN: 0027-8424, DOI: 10.1073/pnas.0607991103 *
COHEN-TANNOUDJI L ET AL: "Measuring the kinetics of biomolecular recognition with magnetic colloids", PHYSICAL REVIEW LETTERS AMERICAN PHYSICAL SOCIETY USA, vol. 100, no. 10, 14 March 2008 (2008-03-14), pages 108301-1 - 108301-4, XP002616465, ISSN: 0031-9007, DOI: doi:10.1103/PhysRevLett.100.108301 *
COHEN-TANNOUDJI LAETITIA ET AL: "Polymer bridging probed by magnetic colloids.", PHYSICAL REVIEW LETTERS 28 JAN 2005 LNKD- PUBMED:15698329, vol. 94, no. 3, 28 January 2005 (2005-01-28), pages 38301-1 - 38301-4, XP002616463, ISSN: 0031-9007, DOI: 10.1103/PhysRevLett.94.038301 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3130994A1 (en) 2021-12-21 2023-06-23 Horiba Abx Sas DOSAGE PROCEDURE
WO2023118692A1 (en) 2021-12-21 2023-06-29 Horiba Abx Sas Assay method using magnetic particles

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Publication number Publication date
FR2959820B1 (en) 2013-03-15

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