FR2470795A1 - Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus - Google Patents

Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus

Info

Publication number
FR2470795A1
FR2470795A1 FR7930135A FR7930135A FR2470795A1 FR 2470795 A1 FR2470795 A1 FR 2470795A1 FR 7930135 A FR7930135 A FR 7930135A FR 7930135 A FR7930135 A FR 7930135A FR 2470795 A1 FR2470795 A1 FR 2470795A1
Authority
FR
France
Prior art keywords
hepatitis
particles
surface antigen
virus
aghbs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7930135A
Other languages
English (en)
Other versions
FR2470795B1 (fr
Inventor
Philippe Adamowicz
Alberte Platel
Ludwig Muller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Priority to FR7930135A priority Critical patent/FR2470795A1/fr
Priority to GB8039069A priority patent/GB2064322B/en
Priority to DE3045990A priority patent/DE3045990C2/de
Priority to US06/213,315 priority patent/US4335214A/en
Priority to JP17192480A priority patent/JPS5692219A/ja
Publication of FR2470795A1 publication Critical patent/FR2470795A1/fr
Application granted granted Critical
Publication of FR2470795B1 publication Critical patent/FR2470795B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10151—Methods of production or purification of viral material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Centrifugal Separators (AREA)

Abstract

L'INVENTION CONCERNE UN PROCEDE DE PURIFICATION DE L'ANTIGENE DE SURFACE DU VIRUS DE L'HEPATITE B (HBS). UNE SUSPENSION DE CET ANTIGENE HBS EST SOUMISE A UNE CENTRIFUGATION DE VELOCITE DANS UN GRADIENT A L'INTERIEUR D'UNE CENTRIFUGEUSE 2, EQUIPEE D'UN NOYAU A DEBIT CONTINU 8. EN MEME TEMPS QUE L'ON REALISE L'OPERATION DE CENTRIFUGATION, ON FAIT CIRCULER LA SUSPENSION DE PARTICULES A PURIFIER DANS UN CIRCUIT FERME 14, DANS LEQUEL EST INTERCALEE LA CENTRIFUGEUSE. ON RECUEILLE ENSUITE LA BANDE CONTENANT L'ANTIGENE HBS PURIFIE, DE FACON EN SOI CONNUE.
FR7930135A 1979-12-07 1979-12-07 Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus Granted FR2470795A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR7930135A FR2470795A1 (fr) 1979-12-07 1979-12-07 Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus
GB8039069A GB2064322B (en) 1979-12-07 1980-12-05 Process for purifying particles of biological origin particularly the surface antigen of the virus of hepatitis b (aghbs)
DE3045990A DE3045990C2 (de) 1979-12-07 1980-12-05 Verfahren zur Reinigung von Teilchen biologischen Ursprungs
US06/213,315 US4335214A (en) 1979-12-07 1980-12-05 Process for purifying particles of biological origin, particularly the surface antigen of the virus of hepatitis B (AgHBs)
JP17192480A JPS5692219A (en) 1979-12-07 1980-12-05 Purification of biologically original particle* particularly antigen *aghbs* of b type hepatitis virus surface

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7930135A FR2470795A1 (fr) 1979-12-07 1979-12-07 Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus

Publications (2)

Publication Number Publication Date
FR2470795A1 true FR2470795A1 (fr) 1981-06-12
FR2470795B1 FR2470795B1 (fr) 1983-07-01

Family

ID=9232533

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7930135A Granted FR2470795A1 (fr) 1979-12-07 1979-12-07 Procede de purification de particules d'origine biologique, notamment de l'antigene de surface du virus de l'hepatite b (aghbs) et les produits obtenus

Country Status (5)

Country Link
US (1) US4335214A (fr)
JP (1) JPS5692219A (fr)
DE (1) DE3045990C2 (fr)
FR (1) FR2470795A1 (fr)
GB (1) GB2064322B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2561256A1 (fr) * 1984-03-16 1985-09-20 Pasteur Institut Procede de purification de particules biologiques de type antigene hbs, par ultracentrifugation de flottation sur gradient de densite

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59101426A (ja) * 1982-11-29 1984-06-12 Green Cross Corp:The Ｂ型肝炎感染予防用ワクチンの製造方法
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
FR2632308B1 (fr) * 1988-06-07 1991-08-16 Fondation Nale Transfusion San Procede et installation de fractionnement en continu de proteines vegetales animales ou humaines
US5462863A (en) * 1989-02-09 1995-10-31 Development Center For Biotechnology Isolation of Hepatitis B surface antigen from transformed yeast cells

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2150559A1 (en) * 1971-06-09 1973-04-13 Merieux Inst Australia antigen purification - for preparation of purified suspensions
FR2248054A1 (fr) * 1973-10-18 1975-05-16 Flockhart & Co
US4024243A (en) * 1975-06-16 1977-05-17 Merck & Co., Inc. Process for isolating hepatitis B antigen
FR2338051A1 (fr) * 1976-01-19 1977-08-12 Community Blood Council Greate Procede de preparation d'antigene d'hepatite b purifie et produits obtenus
DE2709581A1 (de) * 1977-02-23 1978-09-07 Merck & Co Inc Hepatitis b-antigen
US4118477A (en) * 1977-02-14 1978-10-03 Merck & Co., Inc. Hepatitis B antigen
FR2412315A1 (fr) * 1977-02-23 1979-07-20 Merck & Co Inc Procede de preparation d'un antigene de l'hepatite b et l'antigene obtenu
DE2803920A1 (de) * 1978-01-30 1979-08-02 Merck & Co Inc Verfahren zur gewinnung von hepatitis b-oberflaechenantigen
FR2415639A1 (fr) * 1978-01-27 1979-08-24 Merck & Co Inc Antigene de surface de l'hepatite b
GB1554812A (en) * 1977-02-23 1979-10-31 Merck & Co Inc Purifying hepatitis b surface antigen
EP0005408A2 (fr) * 1978-05-08 1979-11-14 Merck & Co. Inc. Procédé d'extraction directe de particules de petites dimensions ayant au plus 50 nm à partir d'un liquide protéinique
EP0012686A1 (fr) * 1978-12-13 1980-06-25 Merck & Co. Inc. Isolation de l'antigène de l'hépatite-Be

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1356413A (en) * 1971-09-09 1974-06-12 Pfizer Ltd Method of obtaining purified australia antigen from blood serum
US3951937A (en) * 1973-12-20 1976-04-20 The Community Blood Council Of Greater New York, Inc. Large scale purification of hepatitis type B antigen using polyethylene glycol
US4017360A (en) * 1975-05-14 1977-04-12 Merck & Co., Inc. Method for purifying hepatitis B antigen
US4088748A (en) * 1976-11-02 1978-05-09 Merck & Co., Inc. Hepatitis B surface antigen
US4102996A (en) * 1977-04-20 1978-07-25 Merck & Co., Inc. Method of preparing hepatitis B core antigen
JPS54101421A (en) * 1978-01-27 1979-08-10 Merck & Co Inc Hepatitis b surface antigen
US4217418A (en) * 1978-05-08 1980-08-12 Merck & Co., Inc. Recovery of small particles by flow centrifugation

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2150559A1 (en) * 1971-06-09 1973-04-13 Merieux Inst Australia antigen purification - for preparation of purified suspensions
FR2248054A1 (fr) * 1973-10-18 1975-05-16 Flockhart & Co
US4024243A (en) * 1975-06-16 1977-05-17 Merck & Co., Inc. Process for isolating hepatitis B antigen
FR2338051A1 (fr) * 1976-01-19 1977-08-12 Community Blood Council Greate Procede de preparation d'antigene d'hepatite b purifie et produits obtenus
US4118477A (en) * 1977-02-14 1978-10-03 Merck & Co., Inc. Hepatitis B antigen
FR2382238A1 (fr) * 1977-02-23 1978-09-29 Merck & Co Inc Procede de purification de l'antigene de l'hepatite b, et produits obtenus
DE2709581A1 (de) * 1977-02-23 1978-09-07 Merck & Co Inc Hepatitis b-antigen
FR2412315A1 (fr) * 1977-02-23 1979-07-20 Merck & Co Inc Procede de preparation d'un antigene de l'hepatite b et l'antigene obtenu
GB1554812A (en) * 1977-02-23 1979-10-31 Merck & Co Inc Purifying hepatitis b surface antigen
GB1554811A (en) * 1977-02-23 1979-10-31 Merck & Co Inc Purifying hepatitis b surface antigen
FR2415639A1 (fr) * 1978-01-27 1979-08-24 Merck & Co Inc Antigene de surface de l'hepatite b
DE2803920A1 (de) * 1978-01-30 1979-08-02 Merck & Co Inc Verfahren zur gewinnung von hepatitis b-oberflaechenantigen
EP0005408A2 (fr) * 1978-05-08 1979-11-14 Merck & Co. Inc. Procédé d'extraction directe de particules de petites dimensions ayant au plus 50 nm à partir d'un liquide protéinique
EP0012686A1 (fr) * 1978-12-13 1980-06-25 Merck & Co. Inc. Isolation de l'antigène de l'hépatite-Be

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1972 *
EXBK/67 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2561256A1 (fr) * 1984-03-16 1985-09-20 Pasteur Institut Procede de purification de particules biologiques de type antigene hbs, par ultracentrifugation de flottation sur gradient de densite

Also Published As

Publication number Publication date
DE3045990C2 (de) 1985-08-22
GB2064322A (en) 1981-06-17
GB2064322B (en) 1983-11-09
DE3045990A1 (de) 1981-09-03
JPS5692219A (en) 1981-07-25
US4335214A (en) 1982-06-15
FR2470795B1 (fr) 1983-07-01
JPH0133157B2 (fr) 1989-07-12

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