FR2443062A1 - Detection rapide des bacteries - Google Patents
Detection rapide des bacteriesInfo
- Publication number
- FR2443062A1 FR2443062A1 FR7921282A FR7921282A FR2443062A1 FR 2443062 A1 FR2443062 A1 FR 2443062A1 FR 7921282 A FR7921282 A FR 7921282A FR 7921282 A FR7921282 A FR 7921282A FR 2443062 A1 FR2443062 A1 FR 2443062A1
- Authority
- FR
- France
- Prior art keywords
- bacteria
- fast detection
- detection
- microbubbles
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/808—Optical sensing apparatus
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
L'invention concerne une méthode de détection rapide de bactéries. Cette méthode consiste à mélanger l'échantillon à tester avec un agent producteur d'enzyme 11, puis avec un conjugué fluorescent 14, à former des microbulles 15 dans un support liquide, et à mettre ledit support liquide sous forme de courant et à déplacer le courant par rapport à un détecteur 25 pour détecter la matière fluorescente à l'intérieur des microbulles ainsi que la présence de bactéries dans l'échantillon de liquide. Application à la détection de bactéries coliformes.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US05/964,622 US4242447A (en) | 1978-11-29 | 1978-11-29 | Rapid detection of bacteria |
Publications (1)
Publication Number | Publication Date |
---|---|
FR2443062A1 true FR2443062A1 (fr) | 1980-06-27 |
Family
ID=25508781
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7921282A Withdrawn FR2443062A1 (fr) | 1978-11-29 | 1979-08-23 | Detection rapide des bacteries |
Country Status (7)
Country | Link |
---|---|
US (1) | US4242447A (fr) |
JP (1) | JPS5574798A (fr) |
AU (1) | AU520828B2 (fr) |
DE (1) | DE2947890A1 (fr) |
FR (1) | FR2443062A1 (fr) |
GB (1) | GB2035372A (fr) |
IT (1) | IT7912553A0 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1982003918A1 (fr) * | 1981-05-04 | 1982-11-11 | Sven Staffan Folestad | Procede de detection de fluorescence induite par un laser de preference dans la chromatographie liquide et dispositif permettant l'execution de ce procede |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5817598B2 (ja) * | 1979-10-31 | 1983-04-08 | 味の素株式会社 | 微生物の迅速検出方法 |
US4643968A (en) * | 1981-01-29 | 1987-02-17 | Massachusetts Institute Of Technology | Process for determining metabolism and growth of cells under various conditions |
US4401755A (en) * | 1981-01-29 | 1983-08-30 | Massachusetts Institute Of Technology | Process for measuring microbiologically active material |
US4399219A (en) * | 1981-01-29 | 1983-08-16 | Massachusetts Institute Of Technology | Process for isolating microbiologically active material |
DE3117241A1 (de) * | 1981-04-30 | 1982-11-18 | Ajinomoto Co., Inc., Tokyo | Schnellverfahren zum nachweis oder zur bestimmung von mikroorganismen |
US4713328A (en) * | 1981-09-30 | 1987-12-15 | Yolken Robert H | Microbial Enzyme assays |
US4492761A (en) * | 1982-04-05 | 1985-01-08 | Duke University | Complement assay method |
US5079144A (en) * | 1982-04-14 | 1992-01-07 | Radiometer Corporate Development Ltd. | Microorganism testing with a hydrolyzable fluorogenic substrate |
JPS6027398A (ja) * | 1983-07-26 | 1985-02-12 | Hitachi Ltd | 微生物検査方法 |
JPS60234597A (ja) * | 1984-05-09 | 1985-11-21 | Terumo Corp | β−ガラクトシダ−ゼ試験用培地 |
CA1298763C (fr) * | 1986-06-16 | 1992-04-14 | Mary Lou Gantzer | Methode de detection rapide de n. gonorrhoeae sans culture |
US5518894A (en) * | 1987-11-05 | 1996-05-21 | Berg; James D. | Rapid coliform detection system |
ATE147790T1 (de) * | 1987-11-05 | 1997-02-15 | James D Berg | Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination |
AT396941B (de) * | 1990-01-08 | 1993-12-27 | Kohlweg Ernst Dr | Verfahren zur bestimmung von phagozytoseaktiven zellen |
US5089392A (en) * | 1990-03-30 | 1992-02-18 | The United States Of America As Represented By Of The Department Of Health And Human Services | Fluorogenic substrates for measurement of lysosomal enzyme activities within intact cells |
DE69121883T2 (de) * | 1990-12-28 | 1997-03-06 | Dade Microscan Inc | Verfahren und zusammensetzung zur bestimmung der antimikrobiellen empfindlichkeit der mehrheit klinisch signifikanter grampositiver organismen |
BR9306104A (pt) * | 1992-03-20 | 1997-11-18 | Celsis Ltd | Método para analisar material em uma amostra líquida dispositivo adaptado para o uso em um método para analisar o material em uma amostra líquida e dispositivo integral |
US5650290A (en) * | 1994-04-01 | 1997-07-22 | Hach Company | Method & Medium for use in detecting E. coli and total coliforms |
US5849515A (en) * | 1994-04-01 | 1998-12-15 | Hach Company | Method and medium for use in detecting E. coli and total coliforms |
US5750357A (en) * | 1994-05-18 | 1998-05-12 | Microquest Diagnostics, Inc. | Method of rapid analyte detection |
US5605812A (en) * | 1995-05-19 | 1997-02-25 | Charm Sciences, Inc. | Test kit and method for the quantitative determination of coliform bacteria and E. coli |
IT1284658B1 (it) * | 1996-06-03 | 1998-05-21 | Isrim S C A R L | Stimolatore dell'attivita' enzimatica in cellule batteriche |
IT1284178B1 (it) * | 1996-06-27 | 1998-05-08 | Isrim S C A R L | Procedimento per la rapida rilevazione in un campione della presenza di cellule batteriche anche se in numero limitatissimo. |
US5968762A (en) * | 1998-03-19 | 1999-10-19 | The University Of Connecticut | Method for detecting bacteria in a sample |
US6555360B1 (en) * | 1998-03-30 | 2003-04-29 | Friedrich Srienc | Flow injection flow cytometry system for on-line monitoring of biroreactors and method for monitoring |
US5972641A (en) * | 1998-08-28 | 1999-10-26 | Colifast Systems Asa | Rapid coliform detection system |
US6511819B2 (en) | 1998-08-28 | 2003-01-28 | Nye Colifast As | Rapid coliform detection system |
DE19841588A1 (de) * | 1998-09-11 | 2000-03-23 | Nitzsche Frank | Verfahren zum Nachweis von Zellen in einer Probe |
US6787302B2 (en) * | 1999-10-25 | 2004-09-07 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
US6673568B1 (en) | 1999-10-25 | 2004-01-06 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
US6649408B2 (en) * | 2000-03-24 | 2003-11-18 | George Mason University | Microdroplet cell culture technique |
BR0003066A (pt) | 2000-07-21 | 2002-04-30 | Fundacao Oswaldo Cruz | Método e dispositivo para detecção microrganismos à fibra óptica |
US20040214314A1 (en) * | 2001-11-02 | 2004-10-28 | Friedrich Srienc | High throughput bioreactor |
US20030138906A1 (en) * | 2001-11-05 | 2003-07-24 | Ingun Tryland | Fluorescence test for measuring heterotrophic bacteria in water |
US9017963B2 (en) * | 2002-01-31 | 2015-04-28 | Woundchek Laboratories (Us), Inc. | Method for detecting microorganisms |
AU2003260815A1 (en) * | 2002-08-08 | 2004-02-25 | University Of Lausanne | Method for the rapid assessment of the presence and viability of bacterial cells and use thereof________________________ |
EP1587950A2 (fr) * | 2003-01-31 | 2005-10-26 | Ethicon, Inc. | Peptides cationiques antimicrobiens et procedes d'utilisation correspondants |
PL215172B1 (pl) * | 2003-01-31 | 2013-10-31 | Ethicon Inc | Sposób wykrywania Escherichia coli, biosensor do wykrywania Escherichia coli i zestaw do wykrywania Escherichia coli |
WO2005042770A2 (fr) | 2003-11-03 | 2005-05-12 | Ethicon, Inc. | Methodes, peptides, et biocapteurs utiles pour detecter un large spectre de bacteries |
AU2004286338A1 (en) * | 2003-11-03 | 2005-05-12 | Ethicon, Inc. | Colorimetric substrates, colorimetric sensors, and methods of use |
US20090270269A1 (en) * | 2008-04-28 | 2009-10-29 | Ashok Kumar | Nano-scale fluoro-biosensors exhibiting a low false alarm rate for rapid detection of biological contaminants |
US20100112630A1 (en) * | 2008-11-03 | 2010-05-06 | Scott Martell Boyette | Methods for measuring microbiological content in aqueous media |
US8481302B2 (en) * | 2008-11-03 | 2013-07-09 | General Electric Company | Total bacteria monitoring system |
WO2020037031A1 (fr) * | 2018-08-17 | 2020-02-20 | Becton, Dickinson And Company | Test de sensibilité antimicrobienne à l'aide de microgouttelettes |
CN109628309A (zh) * | 2018-12-28 | 2019-04-16 | 江苏大学 | 一种耐盐碱反硝化微生物菌株的分离方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3586859A (en) * | 1970-02-16 | 1971-06-22 | Irwin J Katz | Fluorescent cell viability counter |
US3870601A (en) * | 1973-05-04 | 1975-03-11 | Schering Corp | Novel diagnostic system for differentiation of enterobacteriaceae |
US3910702A (en) * | 1974-02-12 | 1975-10-07 | Particle Technology Inc | Apparatus for detecting particles employing apertured light emitting device |
US3951747A (en) * | 1971-09-02 | 1976-04-20 | Hoffmann-La Roche Inc. | Lyophilized media |
US4025393A (en) * | 1975-11-17 | 1977-05-24 | Block Engineering, Inc. | System for detecting growth in microorganisms |
US4126516A (en) * | 1976-11-29 | 1978-11-21 | Corning Glass Works | Method for distinguishing gram positive and gram negative microbes |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3551295A (en) * | 1967-11-29 | 1970-12-29 | Northrop Corp | Microbiological detection and identification system |
US3779907A (en) * | 1970-04-13 | 1973-12-18 | Exxon Research Engineering Co | Liquid membrane process for the separation of aqueous mixtures |
US3864571A (en) * | 1971-02-10 | 1975-02-04 | Wheeler International Inc | Method and Apparatus for Automatically, Identifying and Counting Various Cells in Body Fluids |
US3887812A (en) * | 1973-07-02 | 1975-06-03 | Block Engineering | Method and apparatus for detecting and classifying nucleic acid particles |
US3916197A (en) * | 1973-11-28 | 1975-10-28 | Particle Technology Inc | Method and apparatus for classifying biological cells |
US3957584A (en) * | 1974-09-30 | 1976-05-18 | Warner-Lambert Company | Detection of beta-galactosidase producing micro-organisms |
US4070247A (en) * | 1977-03-30 | 1978-01-24 | Indiana University Foundation | Diagnostic media |
-
1978
- 1978-11-29 US US05/964,622 patent/US4242447A/en not_active Expired - Lifetime
-
1979
- 1979-03-19 AU AU45317/79A patent/AU520828B2/en not_active Expired - Fee Related
- 1979-04-09 JP JP4339579A patent/JPS5574798A/ja active Pending
- 1979-04-20 IT IT7912553A patent/IT7912553A0/it unknown
- 1979-05-08 GB GB7915849A patent/GB2035372A/en not_active Withdrawn
- 1979-08-23 FR FR7921282A patent/FR2443062A1/fr not_active Withdrawn
- 1979-11-28 DE DE19792947890 patent/DE2947890A1/de not_active Withdrawn
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3586859A (en) * | 1970-02-16 | 1971-06-22 | Irwin J Katz | Fluorescent cell viability counter |
US3951747A (en) * | 1971-09-02 | 1976-04-20 | Hoffmann-La Roche Inc. | Lyophilized media |
US3870601A (en) * | 1973-05-04 | 1975-03-11 | Schering Corp | Novel diagnostic system for differentiation of enterobacteriaceae |
US3910702A (en) * | 1974-02-12 | 1975-10-07 | Particle Technology Inc | Apparatus for detecting particles employing apertured light emitting device |
US4025393A (en) * | 1975-11-17 | 1977-05-24 | Block Engineering, Inc. | System for detecting growth in microorganisms |
US4126516A (en) * | 1976-11-29 | 1978-11-21 | Corning Glass Works | Method for distinguishing gram positive and gram negative microbes |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1982003918A1 (fr) * | 1981-05-04 | 1982-11-11 | Sven Staffan Folestad | Procede de detection de fluorescence induite par un laser de preference dans la chromatographie liquide et dispositif permettant l'execution de ce procede |
Also Published As
Publication number | Publication date |
---|---|
AU4531779A (en) | 1980-05-29 |
DE2947890A1 (de) | 1980-06-12 |
US4242447A (en) | 1980-12-30 |
IT7912553A0 (it) | 1979-04-20 |
AU520828B2 (en) | 1982-03-04 |
GB2035372A (en) | 1980-06-18 |
JPS5574798A (en) | 1980-06-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |