ATE147790T1 - Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination - Google Patents

Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination

Info

Publication number
ATE147790T1
ATE147790T1 AT93201622T AT93201622T ATE147790T1 AT E147790 T1 ATE147790 T1 AT E147790T1 AT 93201622 T AT93201622 T AT 93201622T AT 93201622 T AT93201622 T AT 93201622T AT E147790 T1 ATE147790 T1 AT E147790T1
Authority
AT
Austria
Prior art keywords
microorganisms
detecting
low levels
direct method
methylumbelliferone
Prior art date
Application number
AT93201622T
Other languages
English (en)
Inventor
James D Berg
Original Assignee
James D Berg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by James D Berg filed Critical James D Berg
Application granted granted Critical
Publication of ATE147790T1 publication Critical patent/ATE147790T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Radar Systems And Details Thereof (AREA)
AT93201622T 1987-11-05 1988-11-03 Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination ATE147790T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11748187A 1987-11-05 1987-11-05

Publications (1)

Publication Number Publication Date
ATE147790T1 true ATE147790T1 (de) 1997-02-15

Family

ID=22373184

Family Applications (2)

Application Number Title Priority Date Filing Date
AT88909391T ATE117378T1 (de) 1987-11-05 1988-11-03 Schnellverfahren zum nachweis von coliformen mikroorganismen.
AT93201622T ATE147790T1 (de) 1987-11-05 1988-11-03 Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT88909391T ATE117378T1 (de) 1987-11-05 1988-11-03 Schnellverfahren zum nachweis von coliformen mikroorganismen.

Country Status (6)

Country Link
US (1) US5292644A (de)
EP (2) EP0386051B1 (de)
AT (2) ATE117378T1 (de)
AU (1) AU2602488A (de)
DE (2) DE3852825T2 (de)
WO (1) WO1989004372A1 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3811097A1 (de) * 1988-03-31 1989-10-12 Orpegen Med Molekularbioforsch Verfahren zur steuerung biologischer klaerstufen
US5429933A (en) 1986-06-30 1995-07-04 Edberg; Stephen C. Detection of first generation environmental sourced microbes in an environmentally-derived sample
IL85948A0 (en) * 1987-04-08 1988-09-30 Cambridge Bioscience Corp Method and kit for detecting bacteria or fungi
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
AU616957B2 (en) * 1988-06-20 1991-11-14 Becton Dickinson & Company Device for enhancing fluorescence and kinetics and methods of using the device
US6306621B1 (en) * 1991-11-18 2001-10-23 The United States Of America As Represented By The Administrator Of The U.S. Environmental Protection Agency Membrane filter agar medium for simultaneous detection of total coliforms and E. coli
US5650290A (en) * 1994-04-01 1997-07-22 Hach Company Method & Medium for use in detecting E. coli and total coliforms
US5849515A (en) * 1994-04-01 1998-12-15 Hach Company Method and medium for use in detecting E. coli and total coliforms
US5601998A (en) * 1994-08-18 1997-02-11 Minnesota Mining & Mfg Culture medium and device for detection and enumeration of enterobacteriaceae
ATE393236T1 (de) * 1994-11-04 2008-05-15 Idexx Lab Inc Medium zum nachweis bestimmter mikroben in einer probe
US5610029A (en) * 1994-11-04 1997-03-11 Idexx Laboratories, Inc. Medium for detecting target microbes in a sample
US5985594A (en) * 1995-11-14 1999-11-16 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US5700655A (en) * 1995-11-14 1997-12-23 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US7122338B2 (en) * 1995-11-14 2006-10-17 Biocontrol Systems, Inc. Method for quantification of biological material in a sample
IT1284658B1 (it) * 1996-06-03 1998-05-21 Isrim S C A R L Stimolatore dell'attivita' enzimatica in cellule batteriche
IT1284178B1 (it) * 1996-06-27 1998-05-08 Isrim S C A R L Procedimento per la rapida rilevazione in un campione della presenza di cellule batteriche anche se in numero limitatissimo.
US5843699A (en) * 1997-04-08 1998-12-01 Difco Laboratories, Inc. Rapid microorganism detection method
FR2769092B1 (fr) * 1997-09-29 1999-11-12 Lyonnaise Eaux Eclairage Procede de regulation de la desinfection de liquides
WO1999019510A1 (en) 1997-10-10 1999-04-22 President And Fellows Of Harvard College Surface-bound, double-stranded dna protein arrays
US5968762A (en) * 1998-03-19 1999-10-19 The University Of Connecticut Method for detecting bacteria in a sample
US5972641A (en) * 1998-08-28 1999-10-26 Colifast Systems Asa Rapid coliform detection system
US6511819B2 (en) 1998-08-28 2003-01-28 Nye Colifast As Rapid coliform detection system
DE19841588A1 (de) * 1998-09-11 2000-03-23 Nitzsche Frank Verfahren zum Nachweis von Zellen in einer Probe
US6723505B1 (en) 1999-08-13 2004-04-20 Nye Colifast As Method for identification of the indicators of contamination in liquid samples
CA2460212C (en) 2001-09-06 2013-01-22 Genomic Profiling Systems, Inc. Rapid and sensitive detection of cells and viruses
FR2829500B1 (fr) 2001-09-13 2003-12-12 Hemosystem Procede de concentration et de detection de germes pathogenes a partir de produits sanguins et/ou de leurs derives et dispositif pour le mettre en oeuvre
JP3973876B2 (ja) * 2001-10-30 2007-09-12 株式会社三菱化学ヤトロン 新規の細菌分析方法
US20030138906A1 (en) * 2001-11-05 2003-07-24 Ingun Tryland Fluorescence test for measuring heterotrophic bacteria in water
US20030143658A1 (en) * 2002-01-28 2003-07-31 Casella Linda J. Richardson Rapid methods and devices for the detection of coliform and the detection and confirmation of E. coil
FR2845097B1 (fr) * 2002-10-01 2006-06-16 Metis Biotechnologies Procede de detection et de comptage de microorganismes dans un echantillon
US7419797B2 (en) * 2002-10-17 2008-09-02 Conestoga - Rovers And Associates Limited Rapid coliform detection system
ITTR20030002A1 (it) * 2003-07-31 2005-02-01 Isrim Societa Consortile A Respon Sabilita Limit Metodo per la rilevazione di coliformi ed in particolare
DK1725676T4 (en) * 2004-03-01 2016-09-26 Mycometer As Contamination measurement
DE102004011822A1 (de) * 2004-03-11 2005-09-29 Cognis Deutschland Gmbh & Co. Kg Qualitätssicherungssystem zum Nachweis von Mikroorganismen
US8373861B2 (en) * 2004-05-11 2013-02-12 Les Entreprises Biospec Global Solutions Inc. System for rapid analysis of microbiological materials in liquid samples
NZ596586A (en) * 2004-06-23 2012-06-29 Zyzeba Testing Ltd Micro organism test apparatus with multiple puncturable chambers
FR2886651B1 (fr) * 2005-06-03 2007-09-21 Anjou Rech Procede de quantification rapide des micro-organismes coliformes vivants dans un echantillon d'eau
CA2623408C (en) 2005-09-26 2014-04-01 Rapid Micro Biosystems, Inc. Cassette containing growth medium
CN107121396B (zh) 2008-09-24 2020-06-30 曙光生命科学公司 用于检测分析物的方法
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
CA2854000C (en) 2011-11-07 2023-01-24 Rapid Micro Biosystems, Inc. A cell culturing device allowing filtration therein
CA3171698A1 (en) 2012-04-16 2013-10-24 Rapid Micro Biosystems, Inc. Cell culturing device
DE102013225037B4 (de) * 2013-12-05 2016-08-25 Asklepios Kliniken Verwaltungsgesellschaft mbH Verfahren zur Erkennung resistenter Keime und Vorrichtung zum Durchführen desselben
EP3740585B1 (de) 2018-01-17 2023-09-27 Bactobyte Ltd. Verbesserung der detektion von mikroorganismen
CN118048434A (zh) * 2024-04-10 2024-05-17 北京挑战生物技术有限公司 一种快速评估抗菌组分对大肠杆菌抑制能力的方法及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928139A (en) * 1973-02-12 1975-12-23 Wadley Res Inst & Blood Bank Detection of microbial pathogens
US3870601A (en) * 1973-05-04 1975-03-11 Schering Corp Novel diagnostic system for differentiation of enterobacteriaceae
US4070247A (en) * 1977-03-30 1978-01-24 Indiana University Foundation Diagnostic media
FR2405301A1 (fr) * 1977-10-04 1979-05-04 Api Labor Substrats et procede permettant l'identification rapide de bacteries du genre streptococcus
US4242447A (en) * 1978-11-29 1980-12-30 Bioresearch Rapid detection of bacteria
EP0018825B1 (de) * 1979-05-02 1985-02-13 National Research Development Corporation Verfahren zur Identifizierung von Bakterien und Reagenzsatz zur Verwendung in diesem Verfahren
FR2457323A1 (fr) * 1979-05-25 1980-12-19 Api Labor Test permettant la mise en evidence des bacteries du genre salmonella et serratia et leur distinction des genres proteus et providencia
JPS5817598B2 (ja) * 1979-10-31 1983-04-08 味の素株式会社 微生物の迅速検出方法
WO1983003624A1 (en) * 1982-04-14 1983-10-27 Plc Unilever Microbiological test processes and apparatus
US4693972A (en) * 1984-01-16 1987-09-15 Becton, Dickinson And Company Composition and method for rapid detection of microorganisms in clinical samples
FR2558847B1 (fr) * 1984-01-31 1986-06-20 Millipore Sa Dispositif et procede de controle microbiologique de liquides
WO1986005206A1 (en) * 1985-02-27 1986-09-12 University Of Cincinnati Viable microorganism detection by induced fluorescence
US5210022A (en) * 1990-04-20 1993-05-11 Rcr Scientific, Inc. Method test media and chromogenic compounds for identifying and differentiating general coliforms and Escherichia coli bacteria

Also Published As

Publication number Publication date
EP0386051B1 (de) 1995-01-18
EP0574977B1 (de) 1997-01-15
US5292644A (en) 1994-03-08
EP0386051A1 (de) 1990-09-12
ATE117378T1 (de) 1995-02-15
DE3855762T2 (de) 1997-05-07
WO1989004372A1 (en) 1989-05-18
DE3855762D1 (de) 1997-02-27
EP0574977A1 (de) 1993-12-22
AU2602488A (en) 1989-06-01
DE3852825T2 (de) 1995-05-18
DE3852825D1 (de) 1995-03-02

Similar Documents

Publication Publication Date Title
ATE147790T1 (de) Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination
KR970707302A (ko) 대장균군 또는 대장균을 검출하기 위한 효소적 방법(enzymatic method for detecting coliform bacteria or e. coli)
ES2084345T3 (es) Procedimiento y medio de deteccion de salmonelas.
HUP9901198A2 (hu) Eljárás L-aminosavak előállítására
FI892994A0 (fi) Laite fluoresenssin vahvistamiseksi ja kinetiikka ja menetelmiä laitteen käyttämiseksi
FR2844807B1 (fr) Procede de detection de microorganismes resistants a la meticilline
ES8204762A1 (es) Un metodo para preparar acido 2,5-diceto-d-gluconico
JPH09500790A (ja) 炭水化物添加培地を用いて微生物を同定する方法
Williams et al. Enzymes induced in a bacterium by growth on sodium dodecyl sulfate
FI911758A (fi) Mikro-organismien tunnistaminen mittaamalla entsymaattinen aktiivisuus entsyymiaktiivisuuteen vaikuttavien aineiden läsnäollessa
FR2801903B1 (fr) Nouveaux substrats enzymatiques pour la detection de pseudomonas aeruginosa, compositions les contenant et procede de detection de cette espece
WO1994009152A1 (en) Culture medium for the identification of salmonella and process for its use
SE7507975L (sv) Nya kromogena trombinsubstrat
ATE361991T1 (de) Verfahren und agens zur bestimmung von deaminase- aktivität
ATE91711T1 (de) Verfahren zur enzymatischen hydrolyse von alpha- aminoadipinyl-monoaminoverbindungen.
FR2853328B1 (fr) Milieu de detection et/ou d'identification de microorganismes
ITRM930002A1 (it) Antibiogramma e corredo diagnostico per la sua realizzazione.
JPS56125652A (en) Microorganism measuring method by impedance method
SU1056635A1 (ru) Способ выделени биомассы бактерий
SU368298A1 (de)
KR860001885A (ko) 배양미생물의 형광을 이용한 연속적 세포성장 측정방법 및 장치
ES2116229A1 (es) Metodo de diferenciacion bacteriana basado en la determinacion de actividades aminopeptidasicas.
JPS57166995A (en) Liquid medium for diagnosis of candidasis
JPS56164798A (en) Novel method of detecting physiologically active substance
KR880011322A (ko) 히아론산 제조방법

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee