FR2363577A1 - Concn. and purification of plasma antihaemophilic factor - by cryoprecipitation , extn. into water, cooling to ppte. fibrinogen and lyophilising - Google Patents

Concn. and purification of plasma antihaemophilic factor - by cryoprecipitation , extn. into water, cooling to ppte. fibrinogen and lyophilising

Info

Publication number
FR2363577A1
FR2363577A1 FR7626640A FR7626640A FR2363577A1 FR 2363577 A1 FR2363577 A1 FR 2363577A1 FR 7626640 A FR7626640 A FR 7626640A FR 7626640 A FR7626640 A FR 7626640A FR 2363577 A1 FR2363577 A1 FR 2363577A1
Authority
FR
France
Prior art keywords
fibrinogen
concn
factor
ppte
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7626640A
Other languages
French (fr)
Other versions
FR2363577B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RECHERCHES HEMATOLOGIQUES
Original Assignee
RECHERCHES HEMATOLOGIQUES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RECHERCHES HEMATOLOGIQUES filed Critical RECHERCHES HEMATOLOGIQUES
Priority to FR7626640A priority Critical patent/FR2363577A1/en
Publication of FR2363577A1 publication Critical patent/FR2363577A1/en
Application granted granted Critical
Publication of FR2363577B1 publication Critical patent/FR2363577B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Novel process for concn. and purification of Factor VIII (antihaemophilic factor) is claimed. Method comprises (a) extg. a cryopptn. prod. in an aqs. medium of low ionic concn. at approx. ambient temp.; (b) pptg. the fibrinogen from this soln. by a single cooling step. to ca. 1-2 degrees C for >=1/2 hr.; (c) sepg. the supernatant liq. contg. >=80% of the Factor VIII present in the cryopptd. starting prod; and (d) treating the liq. to ensure long-term preservation of a concentrate of the factor. Factor VIII has therapeutic uses and in the prefd. process is obtd. in high purity with reduced losses by selective pptn. of fibrinogen and its denatured and degraded p prods. Process is shorter than prior art (8hrs cf. 16-24hrs) Pref. a supplementary stage is used comprising adding 3-6% of a polyol to the supernatant liq. of (c) and cooling to ca 0-2 degrees C for 1-2 hrs. to ppte. a further amt. of fibrinogen and its denatured and degraded prods.
FR7626640A 1976-09-03 1976-09-03 Concn. and purification of plasma antihaemophilic factor - by cryoprecipitation , extn. into water, cooling to ppte. fibrinogen and lyophilising Granted FR2363577A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7626640A FR2363577A1 (en) 1976-09-03 1976-09-03 Concn. and purification of plasma antihaemophilic factor - by cryoprecipitation , extn. into water, cooling to ppte. fibrinogen and lyophilising

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7626640A FR2363577A1 (en) 1976-09-03 1976-09-03 Concn. and purification of plasma antihaemophilic factor - by cryoprecipitation , extn. into water, cooling to ppte. fibrinogen and lyophilising

Publications (2)

Publication Number Publication Date
FR2363577A1 true FR2363577A1 (en) 1978-03-31
FR2363577B1 FR2363577B1 (en) 1980-05-09

Family

ID=9177361

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7626640A Granted FR2363577A1 (en) 1976-09-03 1976-09-03 Concn. and purification of plasma antihaemophilic factor - by cryoprecipitation , extn. into water, cooling to ppte. fibrinogen and lyophilising

Country Status (1)

Country Link
FR (1) FR2363577A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2440193A1 (en) * 1978-11-01 1980-05-30 Nordisk Insulinlab PROCESS FOR PRODUCING AN ANTI-HEMOPHILIC FACTOR PREPARATION FROM HUMAN BLOOD PLASMA
FR2460305A2 (en) * 1979-06-29 1981-01-23 Merieux Inst PROCESS FOR PREPARING A FACTOR VIII CONCENTRATE
FR2473886A1 (en) * 1980-01-18 1981-07-24 Merieux Inst IMPROVEMENT IN THE METHODS OF CONCENTRATION AND PURIFICATION OF ANTI-HEMOPHILIC FACTOR (FACTOR VIII) AND PREPARATIONS OF FACTOR VIII CONCENTRATE AND PURIFYING THEREOF
EP0083483A1 (en) * 1981-12-14 1983-07-13 Scripps Clinic And Research Foundation Ultrapurificated factor VIII : C preparation
WO2004039382A1 (en) * 2002-10-25 2004-05-13 Shanbrom Technologies, Llc Enhanced production of clotting factors by cryoprecipitation
US11604026B2 (en) 2019-03-14 2023-03-14 Terumo Bct Biotechnologies, Llc Lyophilization loading tray assembly and system
US11634257B2 (en) 2017-10-09 2023-04-25 Terumo Bct Biotechnologies, Llc Lyophilization container and method of using same

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2440193A1 (en) * 1978-11-01 1980-05-30 Nordisk Insulinlab PROCESS FOR PRODUCING AN ANTI-HEMOPHILIC FACTOR PREPARATION FROM HUMAN BLOOD PLASMA
FR2460305A2 (en) * 1979-06-29 1981-01-23 Merieux Inst PROCESS FOR PREPARING A FACTOR VIII CONCENTRATE
WO1984000757A1 (en) * 1979-06-29 1984-03-01 Jacques Liautaud Process for the preparation of a concentrate of factor viii
FR2473886A1 (en) * 1980-01-18 1981-07-24 Merieux Inst IMPROVEMENT IN THE METHODS OF CONCENTRATION AND PURIFICATION OF ANTI-HEMOPHILIC FACTOR (FACTOR VIII) AND PREPARATIONS OF FACTOR VIII CONCENTRATE AND PURIFYING THEREOF
EP0083483A1 (en) * 1981-12-14 1983-07-13 Scripps Clinic And Research Foundation Ultrapurificated factor VIII : C preparation
AU2003295354B2 (en) * 2002-10-25 2008-04-03 Shanbrom Technologies, Llc Enhanced production of clotting factors by cryoprecipitation
WO2004039382A1 (en) * 2002-10-25 2004-05-13 Shanbrom Technologies, Llc Enhanced production of clotting factors by cryoprecipitation
US11634257B2 (en) 2017-10-09 2023-04-25 Terumo Bct Biotechnologies, Llc Lyophilization container and method of using same
US11604026B2 (en) 2019-03-14 2023-03-14 Terumo Bct Biotechnologies, Llc Lyophilization loading tray assembly and system
US11609042B2 (en) 2019-03-14 2023-03-21 Terumo Bct Biotechnologies, Llc Multi-part lyophilization container and method of use
US11609043B2 (en) 2019-03-14 2023-03-21 Terumo Bct Biotechnologies, Llc Lyophilization container fill fixture, system and method of use
US11740019B2 (en) 2019-03-14 2023-08-29 Terumo Bct Biotechnologies, Llc Lyophilization loading tray assembly and system
US11747082B2 (en) 2019-03-14 2023-09-05 Terumo Bct Biotechnologies, Llc Multi-part lyophilization container and method of use
US11815311B2 (en) 2019-03-14 2023-11-14 Terumo Bct Biotechnologies, Llc Lyophilization container fill fixture, system and method of use
US11994343B2 (en) 2019-03-14 2024-05-28 Terumo Bct Biotechnologies, Llc Multi-part lyophilization container and method of use

Also Published As

Publication number Publication date
FR2363577B1 (en) 1980-05-09

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