FR2357901A1 - PLASTIC SUPPORT COATED WITH A RECEIVER FOR LIGAND DETERMINATION - Google Patents

PLASTIC SUPPORT COATED WITH A RECEIVER FOR LIGAND DETERMINATION

Info

Publication number
FR2357901A1
FR2357901A1 FR7720835A FR7720835A FR2357901A1 FR 2357901 A1 FR2357901 A1 FR 2357901A1 FR 7720835 A FR7720835 A FR 7720835A FR 7720835 A FR7720835 A FR 7720835A FR 2357901 A1 FR2357901 A1 FR 2357901A1
Authority
FR
France
Prior art keywords
receptor
plastic support
ligand
receiver
support coated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7720835A
Other languages
French (fr)
Other versions
FR2357901B3 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/702,691 external-priority patent/US4120945A/en
Priority claimed from US05/707,683 external-priority patent/US4105410A/en
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of FR2357901A1 publication Critical patent/FR2357901A1/en
Application granted granted Critical
Publication of FR2357901B3 publication Critical patent/FR2357901B3/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9453—Cardioregulators, e.g. antihypotensives, antiarrhythmics

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)

Abstract

L'invention concerne un procédé pour revêtir un support plastique avec un récepteur d'un ligand en vue de la détermination du ligand, caractérisé en ce que l'on effectue ledit revêtement avec une solution du récepteur contenant un agent bloquant des sites récepteurs pour éviter l'adhérence des sites du récepteur sur le support plastique et d'un agent désagglutinant du récepteur pour éviter l'adhérence du récepteur en agglomérats sur le support plastique, ledit agent bloquant ayant une activité croisée avec le récepteur de 0,0001 à 5 % et pouvant être déplacé par le ligand à déterminer.The invention relates to a method for coating a plastic support with a receptor of a ligand for the determination of the ligand, characterized in that said coating is carried out with a solution of the receptor containing an agent blocking receptor sites in order to avoid the adhesion of the sites of the receptor on the plastic support and of a disagglutinating agent of the receptor to prevent the adhesion of the receptor in agglomerates on the plastic support, said blocking agent having a cross activity with the receptor of 0.0001 to 5% and can be displaced by the ligand to be determined.

FR7720835A 1976-07-06 1977-07-06 PLASTIC SUPPORT COATED WITH A RECEIVER FOR LIGAND DETERMINATION Granted FR2357901A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US05/702,691 US4120945A (en) 1976-07-06 1976-07-06 Substrate coated with receptor and labeled ligand for assays
US05/707,683 US4105410A (en) 1976-07-22 1976-07-22 Receptor coated plastic for assay of ligands

Publications (2)

Publication Number Publication Date
FR2357901A1 true FR2357901A1 (en) 1978-02-03
FR2357901B3 FR2357901B3 (en) 1980-05-16

Family

ID=27107006

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7720835A Granted FR2357901A1 (en) 1976-07-06 1977-07-06 PLASTIC SUPPORT COATED WITH A RECEIVER FOR LIGAND DETERMINATION

Country Status (8)

Country Link
JP (1) JPS54157690A (en)
AU (1) AU505248B2 (en)
BE (1) BE856549A (en)
DE (1) DE2729555C2 (en)
FR (1) FR2357901A1 (en)
GB (1) GB1560860A (en)
IT (1) IT1081127B (en)
SE (1) SE7707812L (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63246667A (en) * 1987-03-31 1988-10-13 Kyoto Ikagaku Kenkyusho:Kk Detection of hemoglobin in excrement
JPS63246668A (en) * 1987-03-31 1988-10-13 Kyoto Ikagaku Kenkyusho:Kk Detection of occult blood in excrement
JPH0441434U (en) * 1990-08-07 1992-04-08

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3790663A (en) * 1970-07-07 1974-02-05 Us Health Preparation of dry antiserum coated solid-phase for radioimmunoassay of antigens
DE2440367C2 (en) * 1973-08-30 1984-03-15 General Electric Co., Schenectady, N.Y. Process for the production of multimolecular layers from immunologically complexed proteins on a substrate

Also Published As

Publication number Publication date
FR2357901B3 (en) 1980-05-16
GB1560860A (en) 1980-02-13
SE7707812L (en) 1978-01-07
IT1081127B (en) 1985-05-16
JPS54157690A (en) 1979-12-12
DE2729555A1 (en) 1978-02-09
AU505248B2 (en) 1979-11-15
BE856549A (en) 1977-10-31
DE2729555C2 (en) 1985-12-12
AU2671177A (en) 1979-01-11

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