ES8703288A1 - Procedimiento de separacion cromatografica de macromoleculasbiologicas - Google Patents

Procedimiento de separacion cromatografica de macromoleculasbiologicas

Info

Publication number
ES8703288A1
ES8703288A1 ES550599A ES550599A ES8703288A1 ES 8703288 A1 ES8703288 A1 ES 8703288A1 ES 550599 A ES550599 A ES 550599A ES 550599 A ES550599 A ES 550599A ES 8703288 A1 ES8703288 A1 ES 8703288A1
Authority
ES
Spain
Prior art keywords
solution
vmf
column
open sections
resin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES550599A
Other languages
English (en)
Other versions
ES550599A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Publication of ES8703288A1 publication Critical patent/ES8703288A1/es
Publication of ES550599A0 publication Critical patent/ES550599A0/es
Expired legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1807—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using counter-currents, e.g. fluidised beds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
    • A23C9/146—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by ion-exchange
    • A23C9/1465—Chromatographic separation of protein or lactose fraction; Adsorption of protein or lactose fraction followed by elution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2215/00—Separating processes involving the treatment of liquids with adsorbents
    • B01D2215/02—Separating processes involving the treatment of liquids with adsorbents with moving adsorbents
    • B01D2215/021—Physically moving or fluidising the adsorbent beads or particles or slurry, excluding the movement of the entire columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)

Abstract

METODO DE SEPARACION CROMATOGRAFICA DE MACROMOLECULAS BIOLOGICAS. CONSISTE EN RELLENAR UNA COLUMNA, QUE CONTIENE ENTRE 2 Y 5 PISOS SUPERPUESTOS, CUYAS BASES CONSTAN DE UN SISTEMA PERFORADO DE DISTRIBUCION, CON UNA RESINA CROMATOGRAFICA, CUYA GRANULOMETRIA MEDIA ES SUPERIOR A 30 MICRONES Y SU MASA VOLUMETRICA ES SUPERIOR A 105 KG/M, SELECTIVA DE LAS MACROMOLECULAS A SEPARAR; ALIMENTAR LA DISOLUCION A LA COLUMNA POR MEDIO DE UN SISTEMA DE DISTRIBUCION, AJUSTANDO EL CAUDAL A UNOS 9 L/H; LAVAR CON AGUA DESTILADA LA RESINA QUE HA FIJADO LAS MACROMOLECULAS, Y ELUIR ESTAS DE LA RESINA CON ACIDO CLORHIDRICO 0,1 N. TIENE APLICACION PARA LA PRODUCCION A ESCALA INDUSTRIAL DE MACROMOLECULAS BIOLOGICAS DE PUREZA ELEVADA.
ES550599A 1985-01-04 1985-12-31 Procedimiento de separacion cromatografica de macromoleculasbiologicas Expired ES8703288A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8500463A FR2575666B1 (fr) 1985-01-04 1985-01-04 Procede et dispositif pour la separation chromatographique de macromolecules biologiques

Publications (2)

Publication Number Publication Date
ES8703288A1 true ES8703288A1 (es) 1987-03-01
ES550599A0 ES550599A0 (es) 1987-03-01

Family

ID=9315250

Family Applications (1)

Application Number Title Priority Date Filing Date
ES550599A Expired ES8703288A1 (es) 1985-01-04 1985-12-31 Procedimiento de separacion cromatografica de macromoleculasbiologicas

Country Status (12)

Country Link
US (1) US4976865A (es)
EP (1) EP0189611B1 (es)
AT (1) ATE42044T1 (es)
AU (1) AU577915B2 (es)
CA (1) CA1278529C (es)
DE (1) DE3569335D1 (es)
DK (1) DK165391C (es)
ES (1) ES8703288A1 (es)
FR (1) FR2575666B1 (es)
IE (1) IE58725B1 (es)
NO (1) NO166148C (es)
NZ (1) NZ214636A (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH674315A5 (es) * 1987-11-23 1990-05-31 Werner Hafner
FR2646993B1 (fr) * 1989-05-19 1993-08-13 Union Coop Agricole Frloyer alain frdupont patrick maschine zum handhaben zerbrechlicher produkte, wie kaesebruch bei der herstellung von kaese.
SE9101149D0 (sv) * 1991-04-17 1991-04-17 Pharmacia Lkb Biotech Beads for down stream processing
FI97277C (fi) * 1993-01-25 1996-11-25 Suomen Sokeri Oy Kromatografinen erotuskolonni, sen sisärakenteet ja kromatografinen erotusmenetelmä
FI96225C (fi) 1993-01-26 1996-05-27 Cultor Oy Menetelmä melassin fraktioimiseksi
US6663780B2 (en) 1993-01-26 2003-12-16 Danisco Finland Oy Method for the fractionation of molasses
US6706188B2 (en) 1993-05-03 2004-03-16 Amersham Biociences Ab Process and means for down stream processing
US5795398A (en) 1994-09-30 1998-08-18 Cultor Ltd. Fractionation method of sucrose-containing solutions
US5902485A (en) 1994-10-03 1999-05-11 Amersham Pharmacia Biotech Ab Access valve devices, their use in separation apparatus and corresponding methods
US6224776B1 (en) 1996-05-24 2001-05-01 Cultor Corporation Method for fractionating a solution
SE9700383D0 (sv) * 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
US20020104801A1 (en) * 1998-04-06 2002-08-08 Nicolas Voute Small dense microporous solid support materials, their preparation,and use for purification of large macromolecules and bioparticles
FI107115B (fi) * 1998-09-30 2001-06-15 Valio Oy Menetelmä naudan insuliinin poistamiseksi
US6610200B1 (en) 1998-10-31 2003-08-26 Amersham Biosciences Ab System and its units
EP1175931A1 (en) * 2000-07-25 2002-01-30 Computer Cell Culture Center S.A. Integration of high cell density bioreactor operation with ultra fast on-line downstream processing
US20040140265A1 (en) * 2000-12-29 2004-07-22 Lihme Allan Otto Fog Extracorporeal capturing of specific bio-macromolecular entities from extracellular body fluids
EP1584242B1 (en) * 2001-06-01 2010-01-20 Upfront Chromatography A/S Fractionation of protein containing mixtures
EP1480524B1 (en) 2002-03-07 2013-04-17 Upfront Chromatography A/S A process of isolating lactoferrin
US7094345B2 (en) * 2002-09-09 2006-08-22 Cytonome, Inc. Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system
CZ2013885A3 (cs) * 2013-11-15 2015-08-19 Univerzita PalackĂ©ho Způsob separace syrovátkových proteinů z mléčného média a zařízení k provádění tohoto způsobu

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2959542A (en) * 1957-03-08 1960-11-08 Dow Chemical Co Continuous gas-lift ion-exchange process and apparatus
GB1070251A (en) * 1962-10-19 1967-06-01 Nat Res Dev Improvements in and relating to solid/fluid contacting
GB1148661A (en) * 1965-05-11 1969-04-16 Victor Pretorius Improvements relating to chromatography
DE1598049B2 (de) * 1965-06-03 1972-04-06 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und vorrichtung zur technischen durchfuehrung der saeulenchromatographie
GB1183833A (en) * 1966-08-11 1970-03-11 Victor Pretorious Improvements in Chromatographic Processes and Apparatus.
US3549526A (en) * 1968-01-31 1970-12-22 Hart Brown Contact process and apparatus
GB1422412A (en) * 1972-01-24 1976-01-28 Chiyoda Chem Eng Construct Co Method of and apparatus for the continuous treatment of water using activated-carbon
US3928193A (en) * 1975-02-14 1975-12-23 Suomen Sokeri Oy Process for large scale chromatography
FR2321932A1 (fr) * 1975-08-28 1977-03-25 Rhone Poulenc Ind Procede de separation de proteines par echange d'ions
US4100149A (en) * 1975-08-28 1978-07-11 Rhone-Poulenc Industries Method of separating proteins by ion exchange
FR2359634A2 (fr) * 1976-07-28 1978-02-24 Rhone Poulenc Ind Procede de separation de proteines par echange d'ions
US4284511A (en) * 1979-08-30 1981-08-18 General Technology Applications, Inc. Process for using magnetically ballasted sorbents
US4443231A (en) * 1982-02-02 1984-04-17 Exxon Research And Engineering Co. Continuous chromatographic separations in a magnetically stabilized fluidized bed
US4675113A (en) * 1984-11-28 1987-06-23 University Patents, Inc. Affinity chromatography using dried calcium alginate-magnetite separation media in a magnetically stabilized fluidized bed

Also Published As

Publication number Publication date
DK165391C (da) 1993-04-13
ATE42044T1 (de) 1989-04-15
DK165391B (da) 1992-11-23
EP0189611B1 (fr) 1989-04-12
US4976865A (en) 1990-12-11
NZ214636A (en) 1989-01-06
DK2186D0 (da) 1986-01-03
CA1278529C (fr) 1991-01-02
ES550599A0 (es) 1987-03-01
FR2575666A1 (fr) 1986-07-11
DK2186A (da) 1986-07-05
AU5161285A (en) 1986-07-10
FR2575666B1 (fr) 1989-08-18
DE3569335D1 (en) 1989-05-18
NO166148B (no) 1991-02-25
AU577915B2 (en) 1988-10-06
NO855317L (no) 1986-07-07
IE860012L (en) 1986-07-04
IE58725B1 (en) 1993-11-03
EP0189611A1 (fr) 1986-08-06
NO166148C (no) 1991-06-05

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