ES8609473A1 - Un metodo para detectar material genetico objetivo - Google Patents

Un metodo para detectar material genetico objetivo

Info

Publication number
ES8609473A1
ES8609473A1 ES542602A ES542602A ES8609473A1 ES 8609473 A1 ES8609473 A1 ES 8609473A1 ES 542602 A ES542602 A ES 542602A ES 542602 A ES542602 A ES 542602A ES 8609473 A1 ES8609473 A1 ES 8609473A1
Authority
ES
Spain
Prior art keywords
methods
genetic material
brand
marked
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES542602A
Other languages
English (en)
Other versions
ES542602A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Biochem Inc
Original Assignee
Enzo Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of ES8609473A1 publication Critical patent/ES8609473A1/es
Publication of ES542602A0 publication Critical patent/ES542602A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODOS PARA DETECTAR UN MATERIAL GENETICO OBJETIVO QUE TIENE UNA SECUENCIA DE BASES O GEN DESEADOS ARN O ADN EN UNA MUESTRA BIOLOGICA Y PARA DETECTAR MUTACIONES, TALES COMO UNA MUTACION PUNTUAL O LA SUPRESION DE UN GEN O BASE. SE DAN TAMBIEN LOS COMPONENTES DE USO EN TALES METODOS. LOS METODOS SE BASAN EN TECNICAS QUE UTILIZAN DOS SEGMENTOS DE POLINUCLEOTIDO DE CADENA SENCILLA MARCADOS, QUE SON COMPLEMENTARIOS CON LA MISMA CADENA O CON CADENAS OPUESTAS DEL MATERIAL GENETICO OBJETIVO. SEGUN ESTOS METODOS SE OBTIENE COMO RESULTADO LA FORMACION DE UN HIBRIDO DOBLE Y UN MULTIHIBRIDO QUE PUEDE DETECTARSE POR VARIOS METODOS DEPENDIENTES DE LA ELECCION DE LA MARCA DE CADA SONDA DE POLINUCLEOTIDO. AQUI CADA SONDA DE POLINUCLEOTIDO SE MARCA CON UNA PARTICULA. LA MARCA HACE A LOS MULTIHIBRIDOS FACILMENTE DETECTABLES.
ES542602A 1984-04-27 1985-04-26 Un metodo para detectar material genetico objetivo Expired ES8609473A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60502284A 1984-04-27 1984-04-27
US65381684A 1984-09-24 1984-09-24

Publications (2)

Publication Number Publication Date
ES8609473A1 true ES8609473A1 (es) 1986-09-01
ES542602A0 ES542602A0 (es) 1986-09-01

Family

ID=27084833

Family Applications (1)

Application Number Title Priority Date Filing Date
ES542602A Expired ES8609473A1 (es) 1984-04-27 1985-04-26 Un metodo para detectar material genetico objetivo

Country Status (11)

Country Link
US (1) US4894325A (es)
EP (2) EP0159719B1 (es)
JP (2) JP2560003B2 (es)
AT (2) ATE91154T1 (es)
AU (2) AU4148685A (es)
CA (1) CA1260372A (es)
DE (2) DE3587427T2 (es)
DK (1) DK188385A (es)
ES (1) ES8609473A1 (es)
IL (1) IL74986A (es)
NO (1) NO165269C (es)

Families Citing this family (122)

* Cited by examiner, † Cited by third party
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US4894325A (en) 1990-01-16
ATE258230T1 (de) 2004-02-15
NO165269B (no) 1990-10-08
NO851689L (no) 1985-10-28
DE3587427T2 (de) 1994-01-20
NO165269C (no) 1991-01-16
EP0159719A3 (en) 1988-03-30
DE3588250D1 (de) 2004-02-26
EP0526912B1 (en) 2004-01-21
EP0526912A2 (en) 1993-02-10
IL74986A (en) 1990-08-31
JPH08336400A (ja) 1996-12-24
IL74986A0 (en) 1985-08-30
AU4148685A (en) 1986-01-30
JP2735822B2 (ja) 1998-04-02
DK188385A (da) 1985-10-28
CA1260372A (en) 1989-09-26
DE3587427D1 (de) 1993-08-05
EP0159719B1 (en) 1993-06-30
ATE91154T1 (de) 1993-07-15
JP2560003B2 (ja) 1996-12-04
EP0159719A2 (en) 1985-10-30
JPS60237361A (ja) 1985-11-26
DK188385D0 (da) 1985-04-26
AU4598489A (en) 1990-03-29
ES542602A0 (es) 1986-09-01
EP0526912A3 (es) 1994-03-02

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