ES8201204A1 - Procedimiento para la determinacion de glicerina - Google Patents

Procedimiento para la determinacion de glicerina

Info

Publication number
ES8201204A1
ES8201204A1 ES499103A ES499103A ES8201204A1 ES 8201204 A1 ES8201204 A1 ES 8201204A1 ES 499103 A ES499103 A ES 499103A ES 499103 A ES499103 A ES 499103A ES 8201204 A1 ES8201204 A1 ES 8201204A1
Authority
ES
Spain
Prior art keywords
determination
reagent
glycerinoxidase
glycerol
dsm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES499103A
Other languages
English (en)
Other versions
ES499103A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES8201204A1 publication Critical patent/ES8201204A1/es
Publication of ES499103A0 publication Critical patent/ES499103A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D231/18—One oxygen or sulfur atom
    • C07D231/20—One oxygen atom attached in position 3 or 5
    • C07D231/22—One oxygen atom attached in position 3 or 5 with aryl radicals attached to ring nitrogen atoms
    • C07D231/26—1-Phenyl-3-methyl-5- pyrazolones, unsubstituted or substituted on the phenyl ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D231/44—Oxygen and nitrogen or sulfur and nitrogen atoms
    • C07D231/46—Oxygen atom in position 3 or 5 and nitrogen atom in position 4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/50—Phenols; Naphthols; Catechols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/96—4-Amino-antipyrine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA LA DETERMINACION ENZIMATICA DE GILCERINA MEDIANTE UNA DETERMINADA GLICERINOXIDASA. SE OXIDA LA GLICERINA CON OXIGENO EN PRESENCIA DE GLICERINOXIDASA, OBTENIDA A PARTIR DE ASPERGILLUS SPC. DSM 1729, Y SE MIDE POLAROMETRICAMENTE EL CONSUMO DE OXIGENO, O BIEN SE DETERMINA ENZIMATICAMENTE. CON CATALASA O PEROXIDASA, EL H2O2 FORMADO. EL ASPERGILLUS SE CULTIVA EN UN MEDIO CELULAR HACIENDO EQUE ESTA SE DISGREGUE, SE SEPARE DE LA PORCION INSOLUBLE Y, ASI, LA GLIVERINOXIDASA SE PRECIPITA CON ACIDO TRICLOROACETICO CON ACETONA, UN FRACCIONAMIENTO CON SULFATO AMONICO Y UNA ABSORCION EN UN INTERCAMBIADOR DE IONES DEBILMENTE BASICO. EL PROCEDIMIENTO ES APROPIADO PARA LA DETERMINACION DE LA GLICERINA O ESTERES DE GLICERINA EN MEDIOS ACUOSOS DE TODO TIPO, TALES COMO EXTRACTOS DE ALIMENTOS LIQUIDOS CORPORALES, Y ESPECIALMENTE, SUERO.
ES499103A 1980-02-05 1981-02-04 Procedimiento para la determinacion de glicerina Granted ES499103A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3004129 1980-02-05

Publications (2)

Publication Number Publication Date
ES8201204A1 true ES8201204A1 (es) 1981-12-16
ES499103A0 ES499103A0 (es) 1981-12-16

Family

ID=6093779

Family Applications (1)

Application Number Title Priority Date Filing Date
ES499103A Granted ES499103A0 (es) 1980-02-05 1981-02-04 Procedimiento para la determinacion de glicerina

Country Status (11)

Country Link
US (1) US4399218A (es)
EP (1) EP0033540B1 (es)
JP (4) JPS6036754B2 (es)
AR (1) AR224669A1 (es)
AT (1) ATE10013T1 (es)
AU (1) AU522705B2 (es)
CA (1) CA1149712A (es)
DD (2) DD201692A5 (es)
DE (1) DE3166756D1 (es)
ES (1) ES499103A0 (es)
SU (1) SU1554765A3 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0048347B1 (de) * 1980-09-19 1983-07-27 Roche Diagnostics GmbH Verfahren und Reagens zur Bestimmung von Glycerin
JPS59140900A (ja) * 1983-01-28 1984-08-13 Toyo Jozo Co Ltd 新規な酵素的高感度測定法
DE3614838A1 (de) * 1986-05-02 1987-11-12 Boehringer Mannheim Gmbh Stabilisierte nad(p)h-abhaengige loesliche nitratreduktase verfahren zu ihrer herstellung und ihre verwendung
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
EP0977869B2 (en) 1997-04-09 2008-11-12 Danisco A/S Lipase and use of same for improving doughs and baked products
WO2000005396A1 (en) 1998-07-21 2000-02-03 Danisco A/S Foodstuff
JP4309137B2 (ja) 2001-05-18 2009-08-05 ダニスコ エイ／エス 酵素を使用した練り粉の調製方法
WO2004059315A1 (en) * 2002-12-24 2004-07-15 Stepan Company Method for determination of free and combined glycerin in biodiesel
US7955814B2 (en) * 2003-01-17 2011-06-07 Danisco A/S Method
DE602004030000D1 (de) 2003-01-17 2010-12-23 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
US20050196766A1 (en) * 2003-12-24 2005-09-08 Soe Jorn B. Proteins
WO2008090395A1 (en) 2007-01-25 2008-07-31 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0405637D0 (en) * 2004-03-12 2004-04-21 Danisco Protein
AU2005263954B2 (en) * 2004-07-16 2011-04-07 Dupont Nutrition Biosciences Aps Enzymatic oil-degumming method
JP5231063B2 (ja) * 2008-03-28 2013-07-10 テルモ株式会社 酸化発色化合物の製造方法
JP5231062B2 (ja) * 2008-03-28 2013-07-10 テルモ株式会社 スルホフェニルジメチルピラゾロンの製造方法
JP5921883B2 (ja) * 2011-12-28 2016-05-24 キリンホールディングス株式会社 微生物醗酵による１４−デヒドロエルゴステロールの生産法
WO2022032148A1 (en) 2020-08-06 2022-02-10 Mate Precision Technologies Inc. Tooling base assembly
US11759914B2 (en) 2020-08-06 2023-09-19 Mate Precision Technologies Inc. Vise assembly

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR195000A1 (es) * 1972-05-17 1973-08-30 Boehringer Mannheim Gmbh Procedimiento para la determinacion de colesterol
CA1101427A (en) * 1976-12-03 1981-05-19 E. Melvin Gindler Determination of triglycerides
JPS6049477B2 (ja) * 1977-04-19 1985-11-01 協和醗酵工業株式会社 グリセロ−ル酸化酵素およびその製造法ならびにグリセロ−ル酸化酵素を用いるグリセロ−ルの定量法
US4098574A (en) * 1977-08-01 1978-07-04 Eastman Kodak Company Glucose detection system free from fluoride-ion interference
US4223090A (en) * 1978-07-13 1980-09-16 American Hospital Supply Corporation Reagents for the enzymatic determination of triglycerides
JPS6031471B2 (ja) * 1978-07-21 1985-07-22 協和醗酵工業株式会社 グリセロ−ル酸化酵素およびその製法
JPS5850719B2 (ja) * 1978-10-18 1983-11-11 協和醗酵工業株式会社 トリグリセライドの定量方法

Also Published As

Publication number Publication date
DE3166756D1 (en) 1984-11-29
DD201692A5 (de) 1983-08-03
EP0033540A2 (de) 1981-08-12
EP0033540B1 (de) 1984-10-24
AU522705B2 (en) 1982-06-24
JPH0254079B2 (es) 1990-11-20
SU1554765A3 (ru) 1990-03-30
JPS56125373A (en) 1981-10-01
JPH0134034B2 (es) 1989-07-17
JPS6036756B2 (ja) 1985-08-22
JPS56121498A (en) 1981-09-24
ATE10013T1 (de) 1984-11-15
EP0033540A3 (en) 1982-09-01
CA1149712A (en) 1983-07-12
AU6693781A (en) 1981-08-27
DD159551A5 (de) 1983-03-16
ES499103A0 (es) 1981-12-16
JPS60259185A (ja) 1985-12-21
AR224669A1 (es) 1981-12-30
JPS60259199A (ja) 1985-12-21
JPS6036754B2 (ja) 1985-08-22
US4399218A (en) 1983-08-16

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19960603