ES483735A1 - Un procedimiento para obtener una preparacion de enzima de glucoamilasa - Google Patents

Un procedimiento para obtener una preparacion de enzima de glucoamilasa

Info

Publication number
ES483735A1
ES483735A1 ES483735A ES483735A ES483735A1 ES 483735 A1 ES483735 A1 ES 483735A1 ES 483735 A ES483735 A ES 483735A ES 483735 A ES483735 A ES 483735A ES 483735 A1 ES483735 A1 ES 483735A1
Authority
ES
Spain
Prior art keywords
production
glucoamylaseand
highly thermostable
thermostable
highly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES483735A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever Bestfoods North America
Original Assignee
Unilever Bestfoods North America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Bestfoods North America filed Critical Unilever Bestfoods North America
Publication of ES483735A1 publication Critical patent/ES483735A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un procedimiento para obtener una preparación de enzima de glucoamilasa que tiene un peso molecular de aproximadamente 31.000, procedimiento que comprende cultivar células de una cepa de Talorornyces duponti en un medio nutriente líquido o sólido constituido por una fuente de carbono, una fuente de nitrógeno o una sal inorgánica, o combinaciones de las mismas, y aislar la preparación de enzima de glucoamilasa del medio de cultivo líquido o sólido por una de las técnicas siguientes: filtración del medio de cultivo líquido para obtener un filtrado constituido por la enzima, y concentración del filtrado bajo presión reducida para obtener la preparación en enzima de glucoamilasa; o salificación de lo preparación de enzima con otras proteínas añadiendo sales inorgánicas al medio de cultivo líquido; o precipitación de la preparación de enzima mediante la adición de un disolvente inorgánico al medio de cultivo líquido; o extracción de la preparación de enzima del medio de cultivo sólido con una solución en agua o una solución tampón antes de concentrar la preparación de enzima extraída bajo presión reducida.
ES483735A 1978-09-01 1979-08-30 Un procedimiento para obtener una preparacion de enzima de glucoamilasa Expired ES483735A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10635478A JPS5534046A (en) 1978-09-01 1978-09-01 Novel glucoamyrase having excellent heat resistance and production

Publications (1)

Publication Number Publication Date
ES483735A1 true ES483735A1 (es) 1980-08-16

Family

ID=14431425

Family Applications (2)

Application Number Title Priority Date Filing Date
ES483735A Expired ES483735A1 (es) 1978-09-01 1979-08-30 Un procedimiento para obtener una preparacion de enzima de glucoamilasa
ES483741A Expired ES483741A1 (es) 1978-09-01 1979-08-30 Un procedimiento para obtener un jarabe de elevado contenidode dextrosa

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES483741A Expired ES483741A1 (es) 1978-09-01 1979-08-30 Un procedimiento para obtener un jarabe de elevado contenidode dextrosa

Country Status (13)

Country Link
US (2) US4247637A (es)
JP (1) JPS5534046A (es)
AR (1) AR221619A1 (es)
BE (1) BE878466A (es)
CA (1) CA1128885A (es)
DE (1) DE2935315A1 (es)
DK (1) DK146631C (es)
ES (2) ES483735A1 (es)
FR (1) FR2434867B1 (es)
IN (1) IN151247B (es)
IT (1) IT1122701B (es)
NL (1) NL7906265A (es)
NZ (1) NZ191172A (es)

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JPS5534046A (en) 1980-03-10
FR2434867A1 (fr) 1980-03-28
US4247637A (en) 1981-01-27
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NZ191172A (en) 1982-02-23
DK146631C (da) 1984-05-07
ES483741A1 (es) 1980-04-16
JPS6155948B2 (es) 1986-11-29
AR221619A1 (es) 1981-02-27
FR2434867B1 (fr) 1986-04-25
NL7906265A (nl) 1980-03-04
DK146631B (da) 1983-11-21
USRE32153E (en) 1986-05-20
IN151247B (es) 1983-03-12
CA1128885A (en) 1982-08-03
IT1122701B (it) 1986-04-23
DK365179A (da) 1980-03-02
DE2935315A1 (de) 1980-03-13

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