ES2627767T3 - Nueva cepa de L. Bulgaricus capaz de inhibir la adhesión de cepas de H. Pylori a células epiteliales - Google Patents

Nueva cepa de L. Bulgaricus capaz de inhibir la adhesión de cepas de H. Pylori a células epiteliales Download PDF

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Publication number
ES2627767T3
ES2627767T3 ES11755445.1T ES11755445T ES2627767T3 ES 2627767 T3 ES2627767 T3 ES 2627767T3 ES 11755445 T ES11755445 T ES 11755445T ES 2627767 T3 ES2627767 T3 ES 2627767T3
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pylori
strains
inhibiting
adhesion
epithelial cells
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Peggy Garault
Gaelle Quere
Raphaëlle BOURDET-SICARD
Francis MEGRAUD
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Gervais Danone SA
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Gervais Danone SA
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/123—Bulgaricus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Una cepa de Lactobacillus delbrueckii subsp. Bulgaricus (L. bulgaricus) caracterizada por que se deposita en la CNCM bajo el número I-4428.

Description

imagen1
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72°C 12 s
Fusión: 95°C 0 s
38°C 50 s 20 ° C/s
2.2 Resultados
Las puntuaciones de infección obtenidas por inmunohistoquímica para las cepas de L. bulgaricus CNCM I-4428 y CNCM I-1632 (Lb 130) se muestran en la Figura 2. Estos resultados muestran que la administración de producto lácteo fermentado con la cepa CNCM I-4428 a ratones infectados por H. pylori disminuye significativamente la
5 puntuación de la infección pero que el tratamiento con el producto lácteo fermentado con la cepa CNCM I-1632 no disminuye la carga de H pylori.
Los resultados obtenidos por PCR en tiempo real para las cepas de L. bulgaricus CNCM I-4428 y CNCM I-1632 (Lb 130) se muestran en la Figura 3. Estos resultados muestran que, en ratones, el tratamiento con el producto lácteo fermentado con la cepa CNCM I-4428 disminuye significativamente la carga de H. pylori pero que el tratamiento con
10 la leche fermentada con la cepa CNCM I-1632 no disminuye la carga de H pylori.
REFERENCIAS
 Boyanova L et al., Lett Appl Microbiol. 2009; 48: 579-84.  Fox JG y Wang TC., J Clin Invest. 2007;117: 60-9.  Gury J et al., Arch Microbiol. 2004;182: 337-45.
15  Lee A et al., Gastroenterology. 1997;112: 1386-97.  Ménard A et al., Antimicrob Agents Chemother. 2002;46:1156-7.  Oleastro M et al., J Clin Microbiol. 2003;41:397-402.  Oleastro M, et al., J Infect Dis. 2008;198:1379-87.  Patnaik R et al., Nat Biotechnol. 2002;20:707-12.
20  Perea Vélez M et al., Appl Environ Microbiol. 2007;73:3595-604.  Polk DB y Peek RM Jr., Nat Rev Cancer. 2010;10:403-14.  Simova ED y col., J Appl Microbiol. 2009;106: 692-701.  Wang Y et al., J Biotechnol. 2007;129:510-5.
8

Claims (1)

  1. imagen1
ES11755445.1T 2011-08-09 2011-08-09 Nueva cepa de L. Bulgaricus capaz de inhibir la adhesión de cepas de H. Pylori a células epiteliales Active ES2627767T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2011/053552 WO2013021239A1 (en) 2011-08-09 2011-08-09 New strain of l. bulgaricus capable of inhibiting the adhesion of h. pylori strains to epithelial cells

Publications (1)

Publication Number Publication Date
ES2627767T3 true ES2627767T3 (es) 2017-07-31

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ID=44645757

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ES11755445.1T Active ES2627767T3 (es) 2011-08-09 2011-08-09 Nueva cepa de L. Bulgaricus capaz de inhibir la adhesión de cepas de H. Pylori a células epiteliales

Country Status (10)

Country Link
US (1) US9272007B2 (es)
EP (1) EP2742125B1 (es)
JP (1) JP5997769B2 (es)
CN (1) CN103987839B (es)
AR (1) AR087463A1 (es)
BR (1) BR112014002911B1 (es)
ES (1) ES2627767T3 (es)
MX (1) MX350212B (es)
RU (1) RU2584600C2 (es)
WO (1) WO2013021239A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9925225B2 (en) 2014-09-26 2018-03-27 Nubiome, Inc. Treatment and prophylaxis for gastroesophageal reflux disease
CN111118106B (zh) * 2019-12-20 2021-12-17 善恩康生物科技（苏州）有限公司 一株可以抑制幽门螺旋杆菌的嗜酸乳杆菌la-10a及其应用
CN116024129A (zh) * 2022-11-29 2023-04-28 光明乳业股份有限公司 一株能够与幽门螺杆菌共聚集的卷曲乳杆菌及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1148064A1 (en) * 2000-04-17 2001-10-24 Vrije Universiteit Brussel Lactobacillus johnsonii bacteriocin, active against Helicobacter pylori
CN101314763B (zh) * 2007-06-01 2011-06-08 统一企业(中国)投资有限公司 具有抗胃肠道致病菌、抗氧化和降血压作用的短双歧杆菌及其用途
JP5679255B2 (ja) * 2009-12-28 2015-03-04 株式会社明治 酸素耐性が増強された微生物およびその利用

Also Published As

Publication number Publication date
CN103987839B (zh) 2016-11-23
CN103987839A (zh) 2014-08-13
JP2014522667A (ja) 2014-09-08
RU2584600C2 (ru) 2016-05-20
MX350212B (es) 2017-08-30
US20140205571A1 (en) 2014-07-24
JP5997769B2 (ja) 2016-09-28
AR087463A1 (es) 2014-03-26
WO2013021239A1 (en) 2013-02-14
US9272007B2 (en) 2016-03-01
BR112014002911A2 (pt) 2017-03-01
EP2742125A1 (en) 2014-06-18
MX2014001572A (es) 2015-03-05
BR112014002911B1 (pt) 2020-12-08
RU2014108605A (ru) 2015-09-20
EP2742125B1 (en) 2017-03-15

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