ES2571340T3 - Methods and compositions for organ cryopreservation - Google Patents

Methods and compositions for organ cryopreservation

Info

Publication number
ES2571340T3
ES2571340T3 ES04784413T ES04784413T ES2571340T3 ES 2571340 T3 ES2571340 T3 ES 2571340T3 ES 04784413 T ES04784413 T ES 04784413T ES 04784413 T ES04784413 T ES 04784413T ES 2571340 T3 ES2571340 T3 ES 2571340T3
Authority
ES
Spain
Prior art keywords
organ
temperature
perfusate
target temperature
perfusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES04784413T
Other languages
Spanish (es)
Inventor
Gregory Fahy
Brian Wowk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
21st Century Medicine Inc
Original Assignee
21st Century Medicine Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 21st Century Medicine Inc filed Critical 21st Century Medicine Inc
Application granted granted Critical
Publication of ES2571340T3 publication Critical patent/ES2571340T3/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un método para el enfriamiento de un órgano desde una temperatura superior a -10 °C hasta una temperatura objetivo entre -10 °C y -40 °C mediante perfusión vascular continua con una solución crioprotectora con lesiones mínimas, comprendiendo dicho método: la perfusión del órgano con un perfusado de solución crioprotectora durante un tiempo insuficiente para que el órgano alcance un equilibrio osmótico aproximado con dicho perfusado de solución crioprotectora; la disminución de la temperatura de dicho perfusado de solución crioprotectora a dicha temperatura objetivo mientras se continúa perfundiendo el órgano; y la continuación de la perfusión de dicho perfusado de solución crioprotectora hasta que la temperatura de al menos una parte de dicho órgano alcanza la temperatura objetivo; en el que la presión de perfusión arterial es 32-110 mmHg, y en el que la concentración arterial es superior en más de 200 mM a la concentración venosa en el momento en que se inicia el enfriamiento por debajo de -10 °C.A method of cooling an organ from a temperature greater than -10°C to a target temperature between -10°C and -40°C by continuous vascular perfusion with a cryoprotective solution with minimal injury, said method comprising: perfusion of the organ with a cryoprotectant solution perfusate for a time insufficient for the organ to reach approximate osmotic equilibrium with said cryoprotectant solution perfusate; lowering the temperature of said cryoprotective solution perfusate to said target temperature while continuing to perfuse the organ; and continuing to perfuse said cryoprotectant solution perfusate until the temperature of at least a portion of said organ reaches the target temperature; in which the arterial perfusion pressure is 32-110 mmHg, and in which the arterial concentration is more than 200 mM higher than the venous concentration at the time cooling below -10 °C is initiated.

ES04784413T 2003-09-16 2004-09-16 Methods and compositions for organ cryopreservation Active ES2571340T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50355103P 2003-09-16 2003-09-16
PCT/US2004/030544 WO2005027633A2 (en) 2003-09-16 2004-09-16 Methods and compositions for the cryopreservation of organs

Publications (1)

Publication Number Publication Date
ES2571340T3 true ES2571340T3 (en) 2016-05-24

Family

ID=34375367

Family Applications (1)

Application Number Title Priority Date Filing Date
ES04784413T Active ES2571340T3 (en) 2003-09-16 2004-09-16 Methods and compositions for organ cryopreservation

Country Status (5)

Country Link
US (1) US8679735B2 (en)
EP (1) EP1675457B1 (en)
CA (1) CA2539274C (en)
ES (1) ES2571340T3 (en)
WO (1) WO2005027633A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0621107A2 (en) * 2005-12-22 2011-11-29 Jane Ennis viable cell retrieval method of umbilical cord tissue, preservation of viability of cells present in umbilical cord tissue, umbilical cord tissue and viable hucpvcs recovery method
ES2633939T3 (en) 2006-05-05 2017-09-26 Tissue Regeneration Therapeutics Inc. Immuno-privileged and modulating progenitor cells
JP5773366B2 (en) 2008-04-21 2015-09-02 ティシュー リジェネレイション セラピューティックス、インコーポレイテッド Human umbilical cord perivascular cells genetically modified for prevention or treatment of biological or chemical agents
BRPI1008009B1 (en) * 2009-02-23 2018-05-29 Cell & Tissue Systems, Inc. Methods for cryopreservation of tissue without ice
EP3177140B1 (en) * 2014-07-29 2022-07-06 21St Century Medicine, Inc. Method of introduction and removal of high concentrations of cryoprotectants by vascular perfusion
CN104161037B (en) * 2014-08-13 2016-01-13 江苏丘陵地区镇江农业科学研究所 A kind of goat sperm glass freezing and thaw formula and method
WO2017066454A2 (en) * 2015-10-14 2017-04-20 X-Therma, Inc. Compositions and methods for reducing ice crystal formation
US10905113B2 (en) 2015-11-12 2021-02-02 Regents Of The University Of Minnesota Compositions and method for storing liquid biospecimens
WO2018022527A1 (en) 2016-07-24 2018-02-01 Fahy Gregory M Methods for introduction and prolonged washout by organ perfusion of maximally vitrifiable cryoprotectant solutions at elevated temperatures
US11246308B2 (en) * 2016-12-20 2022-02-15 Tissue Testing Technologies Llc Ice-free preservation of large volume tissue samples for viable, functional tissue banking
RU2681484C1 (en) * 2018-02-14 2019-03-06 Александр Алексеевич Абрамов Perfusion method for cryo-conservation of human brain
CN109337656B (en) * 2018-11-02 2021-10-22 饶会均 Antifreezing solution for vehicles and preparation method thereof
US11968975B2 (en) 2019-04-30 2024-04-30 Regents Of The University Of Minnesota Compositions and methods for storing liquid biospecimens

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723282A (en) 1991-07-08 1998-03-03 The American National Red Cross Method of preparing organs for vitrification
US5952168A (en) * 1996-11-07 1999-09-14 21St Century Medicine, Inc. Method for vitrification of biological materials using alkoxylated compounds
AU1093900A (en) * 1998-09-21 2000-04-10 21St Century Medicine, Inc. Improved cryoprotectant solutions
AU4308601A (en) 1999-11-30 2001-06-18 21St Century Medicine, Inc. Prevention of ice nucleation by polyglycerol
US6869757B2 (en) 2000-07-31 2005-03-22 21St Century Medicine, Inc. Advantageous carrier solution for vitrifiable concentrations of cryoprotectants, and compatible cryoprotectant mixtures
US6949335B2 (en) 1999-11-30 2005-09-27 21St Century Medicine, Inc. Polyglycerol and lactose compositions for the protection of living systems from states of reduced metabolism
US7250292B2 (en) * 2000-01-26 2007-07-31 21St Century Medicine Hypertonic reduction of chilling injury

Also Published As

Publication number Publication date
EP1675457B1 (en) 2016-02-24
US8679735B2 (en) 2014-03-25
US20070190517A1 (en) 2007-08-16
WO2005027633A3 (en) 2005-06-16
CA2539274C (en) 2016-03-15
EP1675457A4 (en) 2008-09-10
CA2539274A1 (en) 2005-03-31
EP1675457A2 (en) 2006-07-05
WO2005027633A2 (en) 2005-03-31

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