ES2531127T3 - Método de elaboración utilizando proteasas fúngicas o bacterianas - Google Patents
Método de elaboración utilizando proteasas fúngicas o bacterianas Download PDFInfo
- Publication number
- ES2531127T3 ES2531127T3 ES10704371T ES10704371T ES2531127T3 ES 2531127 T3 ES2531127 T3 ES 2531127T3 ES 10704371 T ES10704371 T ES 10704371T ES 10704371 T ES10704371 T ES 10704371T ES 2531127 T3 ES2531127 T3 ES 2531127T3
- Authority
- ES
- Spain
- Prior art keywords
- protease
- activity
- samples
- ice
- incubated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 9
- 239000004365 Protease Substances 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 3
- 102000035195 Peptidases Human genes 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 title 1
- 230000002538 fungal effect Effects 0.000 title 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 abstract 1
- XNPKNHHFCKSMRV-UHFFFAOYSA-N 4-(cyclohexylamino)butane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCCNC1CCCCC1 XNPKNHHFCKSMRV-UHFFFAOYSA-N 0.000 abstract 1
- 239000008000 CHES buffer Substances 0.000 abstract 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 1
- 239000007995 HEPES buffer Substances 0.000 abstract 1
- MKWKNSIESPFAQN-UHFFFAOYSA-N N-cyclohexyl-2-aminoethanesulfonic acid Chemical compound OS(=O)(=O)CCNC1CCCCC1 MKWKNSIESPFAQN-UHFFFAOYSA-N 0.000 abstract 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 abstract 1
- 229920004890 Triton X-100 Polymers 0.000 abstract 1
- 239000013504 Triton X-100 Substances 0.000 abstract 1
- 239000012131 assay buffer Substances 0.000 abstract 1
- 239000012496 blank sample Substances 0.000 abstract 1
- 238000009835 boiling Methods 0.000 abstract 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 abstract 1
- 239000001110 calcium chloride Substances 0.000 abstract 1
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 1
- 235000011148 calcium chloride Nutrition 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
- 229920001187 thermosetting polymer Polymers 0.000 abstract 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C7/00—Preparation of wort
- C12C7/14—Lautering, i.e. clarifying wort
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método de elaboración de una cerveza que comprende la adición de una proteasa termoestable a un mosto después del filtrado pero antes del hervido del mosto, donde termoestable significa que la actividad de la proteasa es al menos el 70% de la actividad de referencia cuando es evaluada según el siguiente método: La proteasa es diluida a 1mg/ml en un tampón de ensayo que comprende 100 mM ácido succínico, 100 mM HEPES, 100 mM CHES, 100 mM CABS, 1 mM CaCl2, 150 mM KCL, 0.01% Tritón X-100, pH ajustado a 5.5 con NaOH. La proteasa es entonces preincubada en i) hielo, ii) 10 min a 70°C. El sustrato para el que la proteasa tiene actividad es suspendido en 0.01% Tritón X-100. Para iniciar la reacción, se añaden 20 μl al tubo y se incuba en un termomezclador Eppendorf a 70°C, 1400 r.p.m. durante 15 min. La reacción se detiene colocando los tubos en hielo. Las muestras son centrifugadas en frío a 14000 g durante 3 min, y se mide el OD590 en el sobrenadante. El valor OD590 de las muestras en blanco es sustraído de los valores correspondientes de las muestras tratadas con proteasa. La termoestabilidad de la proteasa se determina calculando la actividad porcentual de las muestras preincubadas a 70° en comparación con la actividad de referencia, que es la actividad de la proteasa de las muestras incubadas en hielo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09153182 | 2009-02-19 | ||
| PCT/EP2010/052142 WO2010094773A2 (en) | 2009-02-19 | 2010-02-19 | A brewing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2531127T3 true ES2531127T3 (es) | 2015-03-10 |
Family
ID=40834471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10704371T Active ES2531127T3 (es) | 2009-02-19 | 2010-02-19 | Método de elaboración utilizando proteasas fúngicas o bacterianas |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20120058219A1 (es) |
| EP (1) | EP2398893B1 (es) |
| CN (1) | CN102325876A (es) |
| BR (1) | BRPI1013344A2 (es) |
| DK (1) | DK2398893T3 (es) |
| ES (1) | ES2531127T3 (es) |
| PL (1) | PL2398893T3 (es) |
| RU (1) | RU2531522C2 (es) |
| WO (1) | WO2010094773A2 (es) |
| ZA (1) | ZA201105740B (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2097510T3 (en) * | 2006-12-22 | 2017-01-23 | Novozymes As | Process for making a yeast extract |
| US10131864B2 (en) | 2013-04-18 | 2018-11-20 | Novozyme A/S | Polypeptides having protease activity and polynucleotides encoding same |
| WO2014191298A1 (en) * | 2013-05-28 | 2014-12-04 | Novozymes A/S | Polypeptides having protease activity for colloidal stability |
| WO2016193420A1 (en) * | 2015-06-04 | 2016-12-08 | Novozymes A/S | Use of m4 metalloprotease in wort production |
| WO2023099480A2 (en) * | 2021-11-30 | 2023-06-08 | Dsm Ip Assets B.V. | Improved beverage production process |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3795745A (en) * | 1971-03-22 | 1974-03-05 | Schwarz Services Int | Preparation of wort for making beer |
| DE2223656B1 (de) * | 1972-05-16 | 1973-09-27 | Boehringer Sohn Ingelheim | Enzympraeparat und Verfahren zur Herstellung von Bierwuerze |
| DK564086A (da) * | 1986-11-25 | 1988-06-17 | Novo Industri As | Enzymatisk detergent-additiv |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| DE4422198C2 (de) | 1994-06-24 | 1997-08-28 | Audi Ag | Verfahren zum Steuern der elektrischen Beheizung eines Katalysators |
| NZ330940A (en) | 1997-07-24 | 2000-02-28 | F | Production of consensus phytases from fungal origin using computer programmes |
| US6855548B2 (en) * | 2000-02-08 | 2005-02-15 | F. Hoffman-La Roche Ag | Use of acid-stable proteases in animal feed |
| CA2431291C (en) * | 2000-12-07 | 2010-09-21 | Dsm N.V. | Method for the prevention or reduction of haze in beverages |
| WO2003048353A1 (en) | 2001-12-07 | 2003-06-12 | Novozymes A/S | Polypeptides having protease activity and nucleic acids encoding same |
| CN1671833A (zh) * | 2002-07-25 | 2005-09-21 | 诺维信公司 | 糖化方法 |
| EP1594963B1 (en) * | 2003-02-07 | 2007-07-25 | Novozymes A/S | Proteases |
| DE602004027376D1 (de) * | 2003-06-19 | 2010-07-08 | Novozymes As | Proteasen |
| ES2349086T3 (es) * | 2004-05-24 | 2010-12-27 | Novozymes A/S | Enzimas para uso farmacéutico. |
| CA2647327C (en) * | 2006-07-13 | 2016-02-23 | Dsm Ip Assets B.V. | Improved brewing process |
-
2010
- 2010-02-19 RU RU2011138247/10A patent/RU2531522C2/ru not_active IP Right Cessation
- 2010-02-19 CN CN2010800085353A patent/CN102325876A/zh active Pending
- 2010-02-19 DK DK10704371T patent/DK2398893T3/en active
- 2010-02-19 PL PL10704371T patent/PL2398893T3/pl unknown
- 2010-02-19 ES ES10704371T patent/ES2531127T3/es active Active
- 2010-02-19 BR BRPI1013344-5A patent/BRPI1013344A2/pt not_active IP Right Cessation
- 2010-02-19 US US13/147,210 patent/US20120058219A1/en not_active Abandoned
- 2010-02-19 EP EP10704371.3A patent/EP2398893B1/en not_active Revoked
- 2010-02-19 WO PCT/EP2010/052142 patent/WO2010094773A2/en active Application Filing
-
2011
- 2011-08-04 ZA ZA2011/05740A patent/ZA201105740B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI1013344A2 (pt) | 2015-09-01 |
| RU2531522C2 (ru) | 2014-10-20 |
| US20120058219A1 (en) | 2012-03-08 |
| WO2010094773A2 (en) | 2010-08-26 |
| DK2398893T3 (en) | 2015-03-23 |
| PL2398893T3 (pl) | 2015-06-30 |
| CN102325876A (zh) | 2012-01-18 |
| RU2011138247A (ru) | 2013-03-27 |
| ZA201105740B (en) | 2012-04-25 |
| EP2398893B1 (en) | 2014-12-17 |
| WO2010094773A3 (en) | 2010-10-28 |
| EP2398893A2 (en) | 2011-12-28 |
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