ES2528230T3 - Polimerasas libres de detergente - Google Patents
Polimerasas libres de detergente Download PDFInfo
- Publication number
- ES2528230T3 ES2528230T3 ES11161659.5T ES11161659T ES2528230T3 ES 2528230 T3 ES2528230 T3 ES 2528230T3 ES 11161659 T ES11161659 T ES 11161659T ES 2528230 T3 ES2528230 T3 ES 2528230T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- detergent
- detergent free
- free polymerases
- polymerases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Abstract
Mezcla de reacción libre de cualquier detergente que comprende una formulación de una ADN polimerasa termoestable que está completamente libre de detergentes, que comprende Tris/HCl 10 a 50 mM, EDTA 0,05-0,2 mM, DTT 0,5-2 mM, cloruro de potasio 50-200 mM y glicerol al 20-80 %, - un ácido nucleico de molde, que preferentemente es un ADN, - al menos un cebador que es un oligonucleótido que se puede unir a dicho ADN, y - 4 dNTP, que comprende adicionalmente al menos un par de sondas de hibridación de FRET.
Description
Claims (1)
-
imagen1
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10159673 | 2010-04-12 | ||
EP10159673 | 2010-04-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2528230T3 true ES2528230T3 (es) | 2015-02-06 |
Family
ID=42358337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES11161659.5T Active ES2528230T3 (es) | 2010-04-12 | 2011-04-08 | Polimerasas libres de detergente |
Country Status (6)
Country | Link |
---|---|
US (2) | US20110250598A1 (es) |
EP (1) | EP2374874B1 (es) |
JP (1) | JP2011217746A (es) |
CN (1) | CN102212506A (es) |
CA (1) | CA2737015A1 (es) |
ES (1) | ES2528230T3 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2015050980A (ja) * | 2013-09-09 | 2015-03-19 | 東洋紡株式会社 | 非特異増幅を低減させる方法 |
CN107075544B (zh) | 2014-07-22 | 2021-01-29 | 生物辐射实验室股份有限公司 | 与聚合酶联用的缓冲液 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3016032A1 (de) | 1980-04-25 | 1981-10-29 | E.G.O. Regeltechnik GmbH, 6833 Waghäusel | Schalt- und regelvorrichtung |
US5127155A (en) | 1987-12-04 | 1992-07-07 | Kendic Michael W | Method of tension expanding tube to plate and apparatus therefor |
IL88923A (en) * | 1988-01-12 | 1995-07-31 | Hoffmann La Roche | Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification |
JP2531246B2 (ja) * | 1988-08-26 | 1996-09-04 | 東洋紡績株式会社 | 耐熱性dnaポリメラ―ゼおよびその製法 |
US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
JP4335975B2 (ja) | 1995-06-07 | 2009-09-30 | ギリード・サイエンシズ・インコーポレーテッド | Dnaポリメラーゼに対する結合・阻害核酸リガンド |
US5814506A (en) | 1995-08-02 | 1998-09-29 | New England Biolabs, Inc. | Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion |
US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
DE19611759A1 (de) * | 1996-03-25 | 1997-10-02 | Boehringer Mannheim Gmbh | Thermostabile DNA-Polymerase aus Thermococcus spec. |
ATE428801T1 (de) | 1996-06-04 | 2009-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
DE69738605T2 (de) | 1996-06-04 | 2009-04-30 | University Of Utah Research Foundation, Salt Lake City | Behälter zum Durchführen und Beobachten biologischer Prozesse |
US20030134292A1 (en) * | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
WO2008013885A2 (en) * | 2006-07-25 | 2008-01-31 | Stratagene California | Zwitterionic detergents for the storage and use of dna polymerases |
US7972828B2 (en) | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
AU2008263931A1 (en) * | 2007-06-13 | 2008-12-18 | Ge Healthcare Bio-Sciences Corp | Polymerase stabilization |
EP2075331A1 (en) * | 2007-12-28 | 2009-07-01 | Qiagen GmbH | Modified enzymes and their uses |
-
2011
- 2011-03-08 US US13/042,628 patent/US20110250598A1/en not_active Abandoned
- 2011-04-08 ES ES11161659.5T patent/ES2528230T3/es active Active
- 2011-04-08 CA CA2737015A patent/CA2737015A1/en not_active Abandoned
- 2011-04-08 EP EP11161659.5A patent/EP2374874B1/en active Active
- 2011-04-08 JP JP2011085864A patent/JP2011217746A/ja active Pending
- 2011-04-11 CN CN2011100895569A patent/CN102212506A/zh active Pending
-
2014
- 2014-01-21 US US14/160,453 patent/US10227642B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
US20110250598A1 (en) | 2011-10-13 |
CA2737015A1 (en) | 2011-10-12 |
US10227642B2 (en) | 2019-03-12 |
CN102212506A (zh) | 2011-10-12 |
EP2374874A3 (en) | 2012-01-11 |
JP2011217746A (ja) | 2011-11-04 |
EP2374874A2 (en) | 2011-10-12 |
US20140134633A1 (en) | 2014-05-15 |
EP2374874B1 (en) | 2014-11-26 |
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