ES2527426T3 - Detección combinada de agentes biológicos - Google Patents
Detección combinada de agentes biológicos Download PDFInfo
- Publication number
- ES2527426T3 ES2527426T3 ES05748001.4T ES05748001T ES2527426T3 ES 2527426 T3 ES2527426 T3 ES 2527426T3 ES 05748001 T ES05748001 T ES 05748001T ES 2527426 T3 ES2527426 T3 ES 2527426T3
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- Prior art keywords
- marker
- probe
- mixture
- absence
- detecting
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Un método para detectar al menos un primer y un segundo agentes biológicos, comprendiendo el primer agente un primer y un segundo marcadores, y comprendiendo el segundo agente un tercer y un cuarto marcadores, comprendiendo el método: (a) preparar una primera y una segunda mezclas a partir de al menos una y la misma muestra que se sospecha que contiene los agentes; (b) detectar la presencia o la ausencia del primer y del tercer marcadores en la primera mezcla en un primer recipiente; y (c) detectar la presencia o la ausencia del segundo y del cuarto marcadores en la segunda mezcla en un segundo recipiente; mediante lo cual la presencia del primer y del segundo marcadores indica la presencia del primer agente biológico en la muestra, y la presencia del tercer y del cuarto marcadores indica la presencia del segundo agente biológico en la muestra, en donde: la primera mezcla comprende una primera sonda que reconoce específicamente el primer marcador y una tercera sonda que reconoce específicamente el tercer marcador; la segunda mezcla comprende una segunda sonda que reconoce específicamente el segundo marcador y una cuarta sonda que reconoce específicamente el cuarto marcador; cada una de las sondas tiene un marcador detectable, el marcador detectable de la primera sonda es igual que el marcador detectable de la segunda sonda, y el marcador detectable de la tercera sonda es diferente del marcador detectable de la cuarta sonda; la etapa de detección (b) comprende determinar la presencia o la ausencia de la primera y de la tercera sondas que se unen al primer y al tercer marcadores en la primera mezcla; la etapa de detección (c) comprende determinar la presencia o la ausencia de la segunda y de la cuarta sondas que se unen al segundo y al cuarto marcadores en la segunda mezcla.
Description
Claims (1)
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imagen1 imagen2 imagen3
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US56920904P | 2004-05-07 | 2004-05-07 | |
US569209P | 2004-05-07 | ||
PCT/US2005/016257 WO2005111243A2 (en) | 2004-05-07 | 2005-05-06 | Multiplexed detection of biological agents |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2527426T3 true ES2527426T3 (es) | 2015-01-23 |
Family
ID=35394752
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES05748001.4T Active ES2527426T3 (es) | 2004-05-07 | 2005-05-06 | Detección combinada de agentes biológicos |
Country Status (5)
Country | Link |
---|---|
US (1) | US7294466B2 (es) |
EP (1) | EP1745155B1 (es) |
JP (1) | JP4951505B2 (es) |
ES (1) | ES2527426T3 (es) |
WO (1) | WO2005111243A2 (es) |
Families Citing this family (55)
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WO2006069305A2 (en) * | 2004-12-22 | 2006-06-29 | University Of Virginia Patent Foundation | The use of microwaves for thermal and non-thermal applications in micro and nanoscale devices |
WO2007030240A2 (en) * | 2005-08-01 | 2007-03-15 | University Of Virginia Patent Foundation | Microdevices for chemical sensing and chemical actuation |
US8220493B2 (en) * | 2005-08-23 | 2012-07-17 | University Of Virginia Patent Foundation | Passive components for micro-fluidic flow profile shaping and related method thereof |
US20110033922A1 (en) * | 2005-10-04 | 2011-02-10 | Landers James P | Microchip-based acoustic trapping or capture of cells for forensic analysis and related method thereof |
US8916375B2 (en) | 2005-10-12 | 2014-12-23 | University Of Virginia Patent Foundation | Integrated microfluidic analysis systems |
US7754148B2 (en) | 2006-12-27 | 2010-07-13 | Progentech Limited | Instrument for cassette for sample preparation |
US7727473B2 (en) | 2005-10-19 | 2010-06-01 | Progentech Limited | Cassette for sample preparation |
WO2008018905A2 (en) * | 2006-01-17 | 2008-02-14 | Cellumen, Inc. | Method for predicting biological systems responses |
JP5406019B2 (ja) | 2006-05-17 | 2014-02-05 | セルーメン、インコーポレイテッド | 自動化組織分析のための方法 |
JP5340167B2 (ja) | 2006-12-21 | 2013-11-13 | ジェン−プロウブ インコーポレイテッド | 核酸増幅のための方法および組成物 |
JP5211790B2 (ja) * | 2007-03-26 | 2013-06-12 | 住友化学株式会社 | Dnaメチル化測定方法 |
EP2336358A1 (en) | 2008-05-06 | 2011-06-22 | QIAGEN GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
US20100113983A1 (en) * | 2008-10-31 | 2010-05-06 | Microsoft Corporation | Utilizing ultrasound to disrupt pathogens |
US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
EP2412020B1 (en) | 2009-03-24 | 2020-09-30 | University Of Chicago | Slip chip device and methods |
US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
US8512955B2 (en) | 2009-07-01 | 2013-08-20 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
EP3150690B1 (en) | 2010-02-23 | 2022-04-06 | Luminex Corporation | Instrument for the manipulation of cartridges to perform nucleic acid isolation and amplification processes |
EP2686688B1 (en) | 2011-03-17 | 2019-05-08 | Cernostics, Inc. | Systems and compositions for diagnosing barrett's esophagus and methods of using the same |
US8877909B2 (en) | 2011-04-04 | 2014-11-04 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantitation, monitoring, screening, and sequencing of group B Streptococcus |
EP3081301B1 (en) | 2011-05-04 | 2019-08-14 | Luminex Corporation | Apparatus and methods for integrated sample preparation, reaction and detection |
JP6255353B2 (ja) | 2012-02-03 | 2017-12-27 | カリフォルニア インスティチュート オブ テクノロジー | 多重生化学アッセイにおける信号エンコードおよびデコード |
JP6375230B2 (ja) | 2012-02-27 | 2018-08-15 | セルラー リサーチ, インコーポレイテッド | 分子計数のための組成物およびキット |
CN104662172B (zh) | 2012-08-03 | 2018-07-06 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
US9987576B2 (en) | 2012-12-10 | 2018-06-05 | University Of Virginia Patent Foundation | Frequency-based filtering of mechanical actuation using fluidic device |
GB2525104B (en) | 2013-08-28 | 2016-09-28 | Cellular Res Inc | Massively Parallel Single Cell Nucleic Acid Analysis |
WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
US11535882B2 (en) | 2015-03-30 | 2022-12-27 | Becton, Dickinson And Company | Methods and compositions for combinatorial barcoding |
EP3286326A1 (en) | 2015-04-23 | 2018-02-28 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
US10662484B1 (en) | 2016-03-24 | 2020-05-26 | Bench in a Box, LLC | Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria |
US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
EP3635135A1 (en) | 2017-06-05 | 2020-04-15 | Becton, Dickinson and Company | Sample indexing for single cells |
US11721412B2 (en) * | 2017-10-23 | 2023-08-08 | Nautilus Subsidiary, Inc. | Methods for identifying a protein in a sample of unknown proteins |
EP3788170A1 (en) | 2018-05-03 | 2021-03-10 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
EP3821034A4 (en) * | 2018-07-11 | 2022-04-27 | Chromacode, Inc. | METHODS FOR QUANTIFYING ANALYTES IN MULTIPLEXED BIOCHEMICAL REACTIONS |
EP3861134A1 (en) | 2018-10-01 | 2021-08-11 | Becton, Dickinson and Company | Determining 5' transcript sequences |
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EP3894552A1 (en) | 2018-12-13 | 2021-10-20 | Becton, Dickinson and Company | Selective extension in single cell whole transcriptome analysis |
EP4242322A3 (en) | 2019-01-23 | 2023-09-20 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
CN114051534A (zh) | 2019-07-22 | 2022-02-15 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
EP4090763A1 (en) | 2020-01-13 | 2022-11-23 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司(Us) | 用于免疫组库谱分析的引物 |
US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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US5994069A (en) * | 1996-01-24 | 1999-11-30 | Third Wave Technologies, Inc. | Detection of nucleic acids by multiple sequential invasive cleavages |
RU2329305C2 (ru) * | 2001-03-01 | 2008-07-20 | Дзе Джонс Хопкинс Юниверсити | Количественный анализ, позволяющий одновременно обнаруживать и идентифицировать бактериальные инфекции |
EP1288308A1 (en) * | 2001-08-28 | 2003-03-05 | Roche Diagnostics GmbH | A method for the determination of multiple analytes |
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2005
- 2005-05-06 US US11/123,363 patent/US7294466B2/en active Active
- 2005-05-06 EP EP05748001.4A patent/EP1745155B1/en active Active
- 2005-05-06 ES ES05748001.4T patent/ES2527426T3/es active Active
- 2005-05-06 JP JP2007511717A patent/JP4951505B2/ja active Active
- 2005-05-06 WO PCT/US2005/016257 patent/WO2005111243A2/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
US7294466B2 (en) | 2007-11-13 |
WO2005111243A3 (en) | 2007-03-01 |
JP4951505B2 (ja) | 2012-06-13 |
EP1745155B1 (en) | 2014-10-15 |
WO2005111243A2 (en) | 2005-11-24 |
EP1745155A4 (en) | 2008-05-28 |
JP2007535945A (ja) | 2007-12-13 |
US20050250146A1 (en) | 2005-11-10 |
EP1745155A2 (en) | 2007-01-24 |
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