ES2501015T3 - Procedimiento de preparación y purificación de fosfatidilserina - Google Patents
Procedimiento de preparación y purificación de fosfatidilserina Download PDFInfo
- Publication number
- ES2501015T3 ES2501015T3 ES11171047.1T ES11171047T ES2501015T3 ES 2501015 T3 ES2501015 T3 ES 2501015T3 ES 11171047 T ES11171047 T ES 11171047T ES 2501015 T3 ES2501015 T3 ES 2501015T3
- Authority
- ES
- Spain
- Prior art keywords
- phosphatidylserine
- purification
- preparation procedure
- pld
- serine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 title abstract 3
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J7/00—Phosphatide compositions for foodstuffs, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/66—Phosphorus compounds
- A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
- A61K31/685—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Neurology (AREA)
- Biophysics (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Neurosurgery (AREA)
- Dermatology (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
Abstract
Procedimiento de separación y purificación de fosfatidilserina (PS) de fórmula en la que R1 y R2 representan independientemente un acilo C10-C30, saturado, mono-insaturado o poliinsaturado, X>=OH u OM, en la que M >= metal alcalino o alcalinotérreo, amonio, alquilamonio (incluyendo la sal interna) de serina en forma D, L o racémica, a partir de un compuesto de fórmula en la que R1 y R2 y X tienen los significados especificados anteriormente, R3>=CH2-CH2-NH2 o CH2-CH2-N+(CH3)3 y de una enzima fosfolipasa D (PLD) que queda en un medio en el que la reacción de transfofatidilación ha tenido lugar, estando dicho medio seleccionado de: -un medio hidroalcohólico que contiene un alcohol alifático y en presencia de óxido de metal bivalente; -un medio aprótico que consiste en disolventes acuosos/polares y en presencia de un óxido de metal bivalente; o un sistema de dos fases formado por disolvente acuoso/orgánico y en presencia de un óxido de metal bivalente, implicando dicho procedimiento las siguientes etapas: I) añadir una solución salina de cloruro de sodio al medio de reacción que contiene la PS que se ha producido, con la subsiguiente mezcla y separación de la PS; II) recogida y eliminación del sobrenadante; III) las etapas I y II pueden repetirse modificando la concentración de partida de solución de cloruro de sodio y eliminando el sobrenadante; IV) añadir una solución de EDTA para quelar los iones presentes en la solución y la mezcla subsiguiente; V) añadir una solución de etanol que consiste en etanol en un porcentaje del 50 al 100 % o una mezcla de agua/acetona que consiste en acetona a un porcentaje del 50 al 95 % con mezclado y sedimentación de la PS subsiguientes; VI) la etapa V se puede repetir modificando el porcentaje de etanol; VII) añadir una solución de etanol que consiste en etanol a un porcentaje del 90 al 100 %; VIII) recogida y eliminación del sobrenadante; IX) secado del producto final.
Description
Claims (1)
-
imagen1 imagen2 imagen3
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITPD20050164 | 2005-05-30 | ||
| IT000164A ITPD20050164A1 (it) | 2005-05-30 | 2005-05-30 | Processo per la preparazione e l'isolamento di fosfatidi |
| US70387005P | 2005-08-01 | 2005-08-01 | |
| US703870P | 2005-08-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2501015T3 true ES2501015T3 (es) | 2014-10-01 |
Family
ID=39449427
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06753893T Active ES2382748T3 (es) | 2005-05-30 | 2006-05-26 | Procedimiento de preparación y aislamiento de fosfátidos |
| ES11171047.1T Active ES2501015T3 (es) | 2005-05-30 | 2006-05-26 | Procedimiento de preparación y purificación de fosfatidilserina |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06753893T Active ES2382748T3 (es) | 2005-05-30 | 2006-05-26 | Procedimiento de preparación y aislamiento de fosfátidos |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US7759095B2 (es) |
| EP (2) | EP1890706B1 (es) |
| JP (1) | JP5113042B2 (es) |
| KR (1) | KR101298560B1 (es) |
| CN (1) | CN101184494B (es) |
| AT (1) | ATE546145T1 (es) |
| AU (1) | AU2006254376B2 (es) |
| BR (1) | BRPI0611316B8 (es) |
| CA (1) | CA2610575C (es) |
| ES (2) | ES2382748T3 (es) |
| HK (1) | HK1119584A1 (es) |
| IL (1) | IL187749A (es) |
| IT (1) | ITPD20050164A1 (es) |
| MX (1) | MX2007015064A (es) |
| NO (1) | NO337009B1 (es) |
| PL (2) | PL2431020T3 (es) |
| PT (2) | PT2431020E (es) |
| WO (1) | WO2006128639A2 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITPD20050164A1 (it) | 2005-05-30 | 2006-11-30 | Fidia Farmaceutici | Processo per la preparazione e l'isolamento di fosfatidi |
| PT2152821T (pt) | 2007-06-05 | 2019-05-31 | Bank Of Canada | Composições de tinta ou de tóner, métodos de utilização e produtos derivados das mesmas |
| CN102485899A (zh) * | 2010-12-03 | 2012-06-06 | 张永志 | 一种制备富含多不饱和双键脂肪酰基的磷脂酰丝氨酸的高品质南极磷虾油的方法 |
| CN102439125B (zh) * | 2010-12-03 | 2014-04-30 | 上海赢贝生物技术有限公司 | 一种制备富含多不饱和双键脂肪酰基的磷脂酰丝氨酸的高品质南极磷虾油的方法 |
| ITPD20120021A1 (it) | 2012-01-26 | 2013-07-27 | Fidia Farmaceutici | "nuove composizioni farmaceutiche contenenti fosfatidilserina e curcumina". |
| CN104327114A (zh) * | 2014-11-06 | 2015-02-04 | 江南大学 | 一种磷脂酰丝氨酸的制备方法 |
| CN112352051A (zh) * | 2019-12-31 | 2021-02-09 | 邦泰生物工程(深圳)有限公司 | 一种提高磷脂酶d转脂活性的方法以及用其生产磷脂酰丝氨酸的方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3869482A (en) * | 1972-07-31 | 1975-03-04 | Etapharm Chem Pharm Lab G M B | Method of producing highly purified phosphatides |
| AT315359B (de) | 1972-07-31 | 1974-05-27 | Etapharm Chem Pharm Lab Ges M | Verfahren zur Herstellung von hochgereinigten Phosphatiden aus tierischen Organen |
| JPS63245684A (ja) | 1987-03-31 | 1988-10-12 | Japanese Res & Dev Assoc Bio Reactor Syst Food Ind | ホスフアチジン酸誘導体の製造法 |
| DE69011739T2 (de) * | 1989-05-26 | 1995-01-19 | Kao Corp | Verfahren zur Herstellung von Phosphatidsäure. |
| IT1260149B (it) * | 1992-04-17 | 1996-03-28 | Fidia Spa | Metodo per la preparazione e purificazione di miscele di fosfolipidi prive di contaminanti da virus non convenzionali |
| JP3053537B2 (ja) | 1994-11-08 | 2000-06-19 | 株式会社ヤクルト本社 | 脳機能改善剤 |
| US5700668A (en) | 1995-12-08 | 1997-12-23 | Italfarmaco Sud S.P.A. | Process for the industrial preparation of phosphatidylserine |
| DE19917249C2 (de) | 1999-02-26 | 2001-09-27 | Meyer Lucas Gmbh & Co | Verfahren zur Herstellung von Phosphatidylserin-Produkten |
| IT1311929B1 (it) | 1999-04-28 | 2002-03-20 | Chemi Spa | Procedimento per la preparazione di fosfatidilserine. |
| DE19926770A1 (de) | 1999-06-11 | 2000-12-14 | Basf Ag | Nukleinsäurefragment und Vektor, enthaltend eine Halogenase, sowie ein Verfahren zur Halogenierung chemischer Verbindungen |
| JP2003502035A (ja) | 1999-06-15 | 2003-01-21 | イサム・リサーチ・デベロツプメント・カンパニー・オブ・ザ・ヘブルー・ユニバーシテイ・オブ・エルサレム | 水性媒体中でのリン脂質の酵素的製造 |
| AU2001261647A1 (en) * | 2000-05-15 | 2001-11-26 | Identix Incorporated | Fingerprint imaging device |
| JP4298902B2 (ja) | 2000-08-09 | 2009-07-22 | 株式会社ヤクルト本社 | リン脂質の製造法 |
| IT1319679B1 (it) | 2000-12-05 | 2003-10-23 | Chemi Spa | Processo di purificazione di fosfatidilserina. |
| ITPD20010031A1 (it) | 2001-02-09 | 2002-08-09 | Fidia Farmaceutici | Procedimento per la preparazione di fosfatidi puri e loro impiego in campo cosmetico, farmaceutico ed alimentare. |
| DE10142014B4 (de) | 2001-08-28 | 2004-11-11 | Degussa Bioactives Deutschland Gmbh & Co. Kg | Verfahren zur Herstellung von Phosphatidylserin |
| DE10149108A1 (de) * | 2001-10-05 | 2003-04-30 | Degussa Bioactives Deutschland | Verwendung von Phosphatidylserin zur Behandlung des Aufmerksamkeits-Defizit-Syndroms (ADHS) |
| US20030124160A1 (en) * | 2001-10-12 | 2003-07-03 | Petrescu Stefana M. | Targeted drug delivery |
| DE10217555A1 (de) * | 2002-04-19 | 2004-02-19 | Degussa Bioactives Deutschland Gmbh | Physiologisch verträgliche, Phospholipid-haltige, stabile und harte Matrix |
| DE10340740A1 (de) | 2003-09-04 | 2005-03-31 | Degussa Food Ingredients Gmbh | Physiologisch aktive Zusammensetzung auf Phosphatidylserin-Basis |
| DE102004002053A1 (de) | 2004-01-15 | 2005-08-04 | Bioghurt Biogarde Gmbh & Co. Kg | Verfahren zur Herstellung von Phosphatidylserin und dessen Reinigung durch Extraktion |
| ITPD20050164A1 (it) | 2005-05-30 | 2006-11-30 | Fidia Farmaceutici | Processo per la preparazione e l'isolamento di fosfatidi |
-
2005
- 2005-05-30 IT IT000164A patent/ITPD20050164A1/it unknown
-
2006
- 2006-05-26 EP EP06753893A patent/EP1890706B1/en active Active
- 2006-05-26 PT PT111710471T patent/PT2431020E/pt unknown
- 2006-05-26 AT AT06753893T patent/ATE546145T1/de active
- 2006-05-26 US US11/922,680 patent/US7759095B2/en active Active
- 2006-05-26 ES ES06753893T patent/ES2382748T3/es active Active
- 2006-05-26 CA CA2610575A patent/CA2610575C/en not_active Expired - Fee Related
- 2006-05-26 MX MX2007015064A patent/MX2007015064A/es active IP Right Grant
- 2006-05-26 AU AU2006254376A patent/AU2006254376B2/en not_active Ceased
- 2006-05-26 WO PCT/EP2006/005030 patent/WO2006128639A2/en not_active Application Discontinuation
- 2006-05-26 JP JP2008513987A patent/JP5113042B2/ja not_active Expired - Fee Related
- 2006-05-26 BR BRPI0611316A patent/BRPI0611316B8/pt not_active IP Right Cessation
- 2006-05-26 PL PL11171047T patent/PL2431020T3/pl unknown
- 2006-05-26 CN CN2006800186998A patent/CN101184494B/zh not_active Expired - Fee Related
- 2006-05-26 ES ES11171047.1T patent/ES2501015T3/es active Active
- 2006-05-26 PT PT06753893T patent/PT1890706E/pt unknown
- 2006-05-26 PL PL06753893T patent/PL1890706T3/pl unknown
- 2006-05-26 KR KR1020077027896A patent/KR101298560B1/ko active IP Right Grant
- 2006-05-26 EP EP11171047.1A patent/EP2431020B1/en active Active
-
2007
- 2007-11-29 NO NO20076158A patent/NO337009B1/no not_active IP Right Cessation
- 2007-11-29 IL IL187749A patent/IL187749A/en active IP Right Grant
-
2008
- 2008-10-28 HK HK08111830.3A patent/HK1119584A1/xx not_active IP Right Cessation
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