ES2174778T3 - Procedimiento de crioconservacion de esperma de pez cebra. - Google Patents

Procedimiento de crioconservacion de esperma de pez cebra.

Info

Publication number
ES2174778T3
ES2174778T3 ES00100720T ES00100720T ES2174778T3 ES 2174778 T3 ES2174778 T3 ES 2174778T3 ES 00100720 T ES00100720 T ES 00100720T ES 00100720 T ES00100720 T ES 00100720T ES 2174778 T3 ES2174778 T3 ES 2174778T3
Authority
ES
Spain
Prior art keywords
esperma
crioconservation
cebra
pez
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES00100720T
Other languages
English (en)
Inventor
Brigitte Dr Walderich
Renate Nordin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EXELIXIS DEUTSCHLAND GmbH
Original Assignee
EXELIXIS DEUTSCHLAND GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EXELIXIS DEUTSCHLAND GmbH filed Critical EXELIXIS DEUTSCHLAND GmbH
Application granted granted Critical
Publication of ES2174778T3 publication Critical patent/ES2174778T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Steroid Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un procedimiento para crioconservación de esperma de Pez Cebra que comprende: a) extraer los testículos de la cavidad del cuerpo de un Pez Cebra macho; b) enjuagar los testículos aislados en la etapa (a) con un crioprotactor para dar una suspensión de esperma; y c) congelar dicha suspensión de esperma, en la que la cantidad de crioprotector utilizado en la etapa (b) es tal que el volumen de la suspensión de esperma resultante es menos que 30 l.
ES00100720T 2000-01-14 2000-01-14 Procedimiento de crioconservacion de esperma de pez cebra. Expired - Lifetime ES2174778T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP00100720A EP1118268B1 (en) 2000-01-14 2000-01-14 Method for cryoconservation of zebrafish sperm

Publications (1)

Publication Number Publication Date
ES2174778T3 true ES2174778T3 (es) 2002-11-16

Family

ID=8167617

Family Applications (1)

Application Number Title Priority Date Filing Date
ES00100720T Expired - Lifetime ES2174778T3 (es) 2000-01-14 2000-01-14 Procedimiento de crioconservacion de esperma de pez cebra.

Country Status (6)

Country Link
EP (1) EP1118268B1 (es)
AT (1) ATE216182T1 (es)
DE (1) DE60000132T2 (es)
DK (1) DK1118268T3 (es)
ES (1) ES2174778T3 (es)
PT (1) PT1118268E (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
AU2002220018A1 (en) 2000-11-29 2002-06-11 Colorado State University System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
MX337304B (es) 2002-08-01 2016-02-24 Xy Llc Sistema de separacion de baja presion para celulas de esperma.
WO2004017041A2 (en) 2002-08-15 2004-02-26 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
EP2306174B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Flow cytometry nozzle for orienting particles and corresponding method
NZ544103A (en) 2003-05-15 2010-10-29 Xy Llc Efficient haploid cell sorting for flow cytometer systems
CN104974983A (zh) 2004-03-29 2015-10-14 英格朗公司 用于授精的***悬浮液
AU2005266930B2 (en) 2004-07-22 2010-09-16 Inguran, Llc Process for enriching a population of sperm cells
CN104483178B (zh) * 2014-11-24 2018-03-30 中国水产科学研究院黄海水产研究所 赤点石斑鱼成鱼染色体的制备方法

Also Published As

Publication number Publication date
DK1118268T3 (da) 2002-08-12
ATE216182T1 (de) 2002-05-15
EP1118268A1 (en) 2001-07-25
PT1118268E (pt) 2002-09-30
EP1118268B1 (en) 2002-04-17
DE60000132D1 (de) 2002-05-23
DE60000132T2 (de) 2002-11-07

Similar Documents

Publication Publication Date Title
ES2174778T3 (es) Procedimiento de crioconservacion de esperma de pez cebra.
BR0207648B1 (pt) tracionador de correia para um sistema de correia de transmissço de forÇa.
ATE374214T1 (de) Monoklonaler anti-cd-40-antikörper
BR0205389B1 (pt) balanceiro de eixo.
MA25996A1 (fr) Inhibiteurs de phosphodiesterase 4.
NO20062062L (no) Procsesser for preparering av forskjellige former for (S)-(+)-clopidogrel bisulfat
DE60202901D1 (de) Instrument zum Einführen einer Intraokularlinse
DE60233774D1 (de) Lasermikroskop
ATE356357T1 (de) Prostatakrebs-marker
BR0208945B1 (pt) dispositivo de mudança com um só eixo.
DK1286317T3 (da) Fremgangsmåde til at kreditere en konto
AU1817902A (en) Method for the preparation of 21-hydroxy-6,19-oxidoprogesterone (21oh-6op)
DE50202945D1 (de) Trag- bzw. führungsvorrichtung für flugzeugkomponenten
PT1339703E (pt) Processo para a preparacao de paclitaxel
DE50110133D1 (de) Stativ, insbesondere für Operationsmikroskope
ITTO20010754A0 (it) Dispositivo di regolazione della posizione di un pedale.
ITTO20010895A0 (it) Dispositivo campionatore, particolarmente per analizzatori elementariautomatici.
DE60039695D1 (de) Antifucoidan antikörper
DE50208050D1 (de) Getriebe für eine Stellvorrichtung
BR0209132A (pt) Braço esborralhador para um forno
IT251804Y1 (it) Pinza compensata per mandrino maschiatore.
BR0108622A (pt) Processo para a n-formilação seletiva de n-hidroxil aminas
DE50212572D1 (de) En weiche
BR0114247B1 (pt) processo para a preparaÇço de tiofenàis.
NO20012002L (no) Anordning ved vippekar

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 1118268

Country of ref document: ES