DE60000132T2 - Verfahren zur Kryokonservierung von Zebrafisch-Samen - Google Patents

Verfahren zur Kryokonservierung von Zebrafisch-Samen

Info

Publication number: DE60000132T2
Authority: DE; Germany
Prior art keywords: sperm; cryoprotectant; zebrafish; fish; testes
Prior art date: 2000-01-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

DE60000132T

Other languages

German (de)

English (en)

Other versions

DE60000132D1 (de

Inventor

Renate Nordin

Dr. Walderich

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Artemis Pharmaceuticals GmbH

Original Assignee

Artemis Pharmaceuticals GmbH

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-01-14

Filing date

2000-01-14

Publication date

2002-11-07

2000-01-14 Application filed by Artemis Pharmaceuticals GmbH filed Critical Artemis Pharmaceuticals GmbH

2002-05-23 Publication of DE60000132D1 publication Critical patent/DE60000132D1/de

2002-11-07 Application granted granted Critical

2002-11-07 Publication of DE60000132T2 publication Critical patent/DE60000132T2/de

2020-01-15 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

238000000034 method Methods 0.000 title claims abstract description 33
241000252212 Danio rerio Species 0.000 title claims abstract description 15
238000005138 cryopreservation Methods 0.000 title abstract description 7
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 44
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 22
239000002577 cryoprotective agent Substances 0.000 claims description 20
238000012360 testing method Methods 0.000 claims description 16
210000001550 testis Anatomy 0.000 claims description 16
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
239000000725 suspension Substances 0.000 claims description 11
239000008267 milk Substances 0.000 claims description 8
235000013336 milk Nutrition 0.000 claims description 8
210000004080 milk Anatomy 0.000 claims description 8
239000012891 Ringer solution Substances 0.000 claims description 7
239000000843 powder Substances 0.000 claims description 7
238000007710 freezing Methods 0.000 claims description 6
230000008014 freezing Effects 0.000 claims description 6
239000007788 liquid Substances 0.000 claims description 6
229910052757 nitrogen Inorganic materials 0.000 claims description 6
108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
239000011521 glass Substances 0.000 claims description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 3
239000003495 polar organic solvent Substances 0.000 claims 3
102000004169 proteins and genes Human genes 0.000 claims 2
108090000623 proteins and genes Proteins 0.000 claims 2
229940098773 bovine serum albumin Drugs 0.000 claims 1
241000251468 Actinopterygii Species 0.000 description 30
230000004720 fertilization Effects 0.000 description 22
239000000243 solution Substances 0.000 description 14
235000013601 eggs Nutrition 0.000 description 11
238000002474 experimental method Methods 0.000 description 11
238000002360 preparation method Methods 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
239000002609 medium Substances 0.000 description 3
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
238000003744 In vitro fertilisation Methods 0.000 description 2
210000001015 abdomen Anatomy 0.000 description 2
235000011089 carbon dioxide Nutrition 0.000 description 2
238000001816 cooling Methods 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
210000001161 mammalian embryo Anatomy 0.000 description 2
239000008188 pellet Substances 0.000 description 2
238000003825 pressing Methods 0.000 description 2
230000002381 testicular Effects 0.000 description 2
235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
240000006497 Dianthus caryophyllus Species 0.000 description 1
239000012980 RPMI-1640 medium Substances 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
229910000397 disodium phosphate Inorganic materials 0.000 description 1
235000019800 disodium phosphate Nutrition 0.000 description 1
230000000694 effects Effects 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
235000013861 fat-free Nutrition 0.000 description 1
239000012894 fetal calf serum Substances 0.000 description 1
238000011534 incubation Methods 0.000 description 1
208000021267 infertility disease Diseases 0.000 description 1
239000000463 material Substances 0.000 description 1
210000000582 semen Anatomy 0.000 description 1
FQZJYWMRQDKBQN-UHFFFAOYSA-N tricaine methanesulfonate Chemical compound CS([O-])(=O)=O.CCOC(=O)C1=CC=CC([NH3+])=C1 FQZJYWMRQDKBQN-UHFFFAOYSA-N 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts

Landscapes

Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Dentistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Environmental Sciences (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Cultivation Of Plants (AREA)
Pretreatment Of Seeds And Plants (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Steroid Compounds (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DE60000132T 2000-01-14 2000-01-14 Verfahren zur Kryokonservierung von Zebrafisch-Samen Expired - Fee Related DE60000132T2 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
EP00100720A EP1118268B1 (en)	2000-01-14	2000-01-14	Method for cryoconservation of zebrafish sperm

Publications (2)

Publication Number	Publication Date
DE60000132D1 DE60000132D1 (de)	2002-05-23
DE60000132T2 true DE60000132T2 (de)	2002-11-07

Family

ID=8167617

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE60000132T Expired - Fee Related DE60000132T2 (de)	2000-01-14	2000-01-14	Verfahren zur Kryokonservierung von Zebrafisch-Samen

Country Status (6)

Country	Link
EP (1)	EP1118268B1 (es)
AT (1)	ATE216182T1 (es)
DE (1)	DE60000132T2 (es)
DK (1)	DK1118268T3 (es)
ES (1)	ES2174778T3 (es)
PT (1)	PT1118268E (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1998034094A1 (en)	1997-01-31	1998-08-06	The Horticulture & Food Research Institute Of New Zealand Ltd.	Optical apparatus
US6149867A (en)	1997-12-31	2000-11-21	Xy, Inc.	Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en)	1999-11-24	2007-04-24	Xy, Inc.	Method of cryopreserving selected sperm cells
US7713687B2 (en)	2000-11-29	2010-05-11	Xy, Inc.	System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043574A2 (en)	2000-11-29	2002-06-06	Xy, Inc.	System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en)	2002-08-01	2013-07-16	Xy, Llc	Heterogeneous inseminate system
CA2532376C (en)	2002-08-01	2015-05-12	Colorado State University Research Foundation	Low pressure sperm cell separation system
JP2005535346A (ja)	2002-08-15	2005-11-24	エックスワイ，インコーポレイテッド	高分解能フローサイトメーター
US7169548B2 (en)	2002-09-13	2007-01-30	Xy, Inc.	Sperm cell processing and preservation systems
DK1608963T3 (da)	2003-03-28	2010-04-06	Inguran Llc	Apparatur og fremgangsmåder til tilvejebringelse af kønssorteret dyresperma
NZ544103A (en)	2003-05-15	2010-10-29	Xy Llc	Efficient haploid cell sorting for flow cytometer systems
BRPI0509488A (pt)	2004-03-29	2007-09-11	Monsanto Technology Llc	suspensões de esperma para seleção de populações enriquecidas carregando cromossomo x ou y
AR049732A1 (es)	2004-07-22	2006-08-30	Monsanto Co	Proceso para enriquecer una poblacion de celulas de esperma
CN104483178B (zh) *	2014-11-24	2018-03-30	中国水产科学研究院黄海水产研究所	赤点石斑鱼成鱼染色体的制备方法

2000
- 2000-01-14 EP EP00100720A patent/EP1118268B1/en not_active Expired - Lifetime
- 2000-01-14 ES ES00100720T patent/ES2174778T3/es not_active Expired - Lifetime
- 2000-01-14 PT PT00100720T patent/PT1118268E/pt unknown
- 2000-01-14 DE DE60000132T patent/DE60000132T2/de not_active Expired - Fee Related
- 2000-01-14 DK DK00100720T patent/DK1118268T3/da active
- 2000-01-14 AT AT00100720T patent/ATE216182T1/de not_active IP Right Cessation

Also Published As

Publication number	Publication date
DE60000132D1 (de)	2002-05-23
EP1118268A1 (en)	2001-07-25
ES2174778T3 (es)	2002-11-16
PT1118268E (pt)	2002-09-30
EP1118268B1 (en)	2002-04-17
ATE216182T1 (de)	2002-05-15
DK1118268T3 (da)	2002-08-12

Legal Events

Date	Code	Title	Description
2003-05-15	8364	No opposition during term of opposition
2003-11-13	8339	Ceased/non-payment of the annual fee

Publication	Publication Date	Title
DE69913186T3 (de)	2012-08-09	Verfahren zur verglasung einer biologischen probe
DE60000132T2 (de)	2002-11-07	Verfahren zur Kryokonservierung von Zebrafisch-Samen
DE602004008241T2 (de)	2008-05-08	Verfahren zum anfärben von sperma
DE60017945T2 (de)	2006-06-08	Verfahren zur kryokonservierung von samenzellen
DE3744113C2 (de)	1998-04-09	Verfahren zur Multiplikation von Rinderembryonen und zum Klonen von Rindern
DE3427553C2 (de)	1994-04-14	Verfahren zum Sichtbarmachen von Genmaterial
DE69938000T2 (de)	2008-04-30	System zur künstlichen nicht-chirurgischen besamung von pferden
US20010023060A1 (en)	2001-09-20	Method for cryoconservation of Zebrafish sperm
Belt et al.	1971	Fine structure of the interstitial cells of Leydig in the squirrel monkey during seasonal regression
DE69018813T2 (de)	1995-08-17	Verfahren zur herstellung eines transgenen; nicht humanen tieres durch das ein- bringen von exogener dna in tierzellen; die somatischen und keimbahn ursprung haben.
Callebaut	1968	Extracorporal development of quail oocytes
DE60133644T2 (de)	2009-05-28	Embryonale stammzellen abkünftig aus affen
DE69526830T2 (de)	2002-12-12	Verfahren zur Tiefkühlkonservierung primordialer Keimzellen und Keimzellen
DE2454674A1 (de)	1975-05-28	Verfahren zur kultivierung zellulaerer koerper, insbesondere bei der aufzucht von saeugetieren
EP0351357B1 (de)	1993-03-03	Verbessertes Fischzuchtverfahren
DE69800966T2 (de)	2002-04-04	Spermaverdünner der natives phosphocaseinat oder beta lactoglobulin enthält, verfahren zu seiner herstellung und seine verwendungen
Breed et al.	1970	Ovulation and associated histological changes in the ovary following coitus in the vole (Microtus agrestis)
DE69714976T2 (de)	2003-05-08	Verwendung von arabinogalaktan für die reinigung eines spermienproduktes
DE2459582A1 (de)	1975-06-26	Verfahren zur gewinnung von ebersamen
DE102012021900A1 (de)	2014-05-15	Verfahren und Konservierungslösung zur Kryokonservierung von Insekten-Sperma
DE69528295T2 (de)	2003-05-08	Anwendung des biofrequenzspektrums in der embryotechnologie bei tieren
DE60034057T2 (de)	2007-12-06	Behandlung von menschlicher unfruchtbarkeit
DE4205386C1 (en)	1992-12-10	Cryo:preservation and revitalisation of fish eggs, crab larvae etc. - involves treating with e.g. DMSO, freezing and revitalising by warming
Hochi et al.	1996	In vitro capacitation of stallion spermatozoa assessed by the lysophosphatidylcholine-induced acrosome reaction and the penetration rate into in vitro-matured, zona-free mare oocytes
CH670930A5 (es)	1989-07-31