ES2132116T3 - Celulas recombinantes que expresan altamente genes heterologos cromosomicamente integrados. - Google Patents

Celulas recombinantes que expresan altamente genes heterologos cromosomicamente integrados.

Info

Publication number
ES2132116T3
ES2132116T3 ES92901454T ES92901454T ES2132116T3 ES 2132116 T3 ES2132116 T3 ES 2132116T3 ES 92901454 T ES92901454 T ES 92901454T ES 92901454 T ES92901454 T ES 92901454T ES 2132116 T3 ES2132116 T3 ES 2132116T3
Authority
ES
Spain
Prior art keywords
integrated
segment
increased expression
host cells
ethanol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92901454T
Other languages
English (en)
Inventor
Lonnie O Ingram
Kazuyoshi Ohta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
Original Assignee
University of Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24501169&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2132116(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of Florida filed Critical University of Florida
Application granted granted Critical
Publication of ES2132116T3 publication Critical patent/ES2132116T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE HAN OBTENIDO CELULAS ANFITRIONAS RECOMBINANTES (A) UN SEGMENTO DE POLINUCLEOTIDO HETEROLOGO, QUE CODIFICA UN POLIPEPTIDO, ESTABLEMENTE INTEGRADO EN UN CROMOSOMA, QUE SE ENCUENTRA BAJO EL CONTROL TRANSCRIPCIONAL DE UN PROMOTOR ENDOGENO Y (B) UNA MUTACION QUE EFECTUA UNA EXPRESION INCREMENTADA DEL SEGMENTO HETEROLOGO, DANDO COMO RESULTADO UNA PRODUCCION MEJORADA MEDIANTE LAS CELULAS ANFITRIONAS DE CADA POLIPEPTIDO CODIFICADO POR ESE SEGMENTO, EN RELACION CON LA PRODUCCION DE CADA POLIPEPTIDO MEDIANTE CELULAS ANFITRIONAS EN LA AUSENCIA DE LA MUTACION. LA EXPRESION INCREMENTADA ASI CONSEGUIDA ES RETENIDA EN AUSENCIA DE LAS CONDICIONES QUE SELECCIONAN LAS CELULAS QUE MUESTRAN DICHA EXPRESION INCREMENTADA. CUANDO EL SEGMENTO INTEGRADO COMPRENDE, POR EJEMPLO, GENES PARA LA PRODUCCION DE ETANOL A PARTIR DE UN PRODUCTOR DE ETANOL COMO LA "ZYMOMONAS MOBILIS", "ESCHERICHIA COLI" RECOMBINANTE Y OTRAS CELULAS ENTERICAS BACTERIANAS DENTRO DE LA INVENCION SON CAPACES DE CONVERTIR UNA AMPLIA GAMA DE AZUCARES DERIVADOS DE LA BIOMASA EN ETANOL DE MANERA EFICIENTE.
ES92901454T 1990-12-07 1991-12-04 Celulas recombinantes que expresan altamente genes heterologos cromosomicamente integrados. Expired - Lifetime ES2132116T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US62422790A 1990-12-07 1990-12-07

Publications (1)

Publication Number Publication Date
ES2132116T3 true ES2132116T3 (es) 1999-08-16

Family

ID=24501169

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92901454T Expired - Lifetime ES2132116T3 (es) 1990-12-07 1991-12-04 Celulas recombinantes que expresan altamente genes heterologos cromosomicamente integrados.

Country Status (12)

Country Link
EP (1) EP0560885B1 (es)
JP (2) JP3593125B2 (es)
AT (1) ATE177784T1 (es)
AU (3) AU668677B2 (es)
BR (1) BR9107210A (es)
CA (1) CA2097803C (es)
DE (1) DE69131015T2 (es)
DK (1) DK0560885T3 (es)
ES (1) ES2132116T3 (es)
FI (1) FI111552B (es)
GR (1) GR3030570T3 (es)
WO (1) WO1992010561A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424202A (en) * 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5968502A (en) * 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
CA2177959C (en) * 1993-12-23 2007-02-13 Gregory Franklin Hollis Homologous recombination antibody expression system for murine cells
EP0966521A1 (en) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
WO2001018222A1 (en) * 1999-09-07 2001-03-15 University Of Florida Methods and compositions for the chromosomal integration of heterologous sequences
KR100814313B1 (ko) * 2000-04-06 2008-03-18 교와 핫꼬 고교 가부시끼가이샤 대장균의 염색체 재조합에 사용되는 플라스미드
US7598063B2 (en) 2000-06-16 2009-10-06 Research Institute Of Innovative Technology For The Earth Process for producing ethanol by using recombinant coryneform bacterium
US6566107B1 (en) * 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
WO2008021141A2 (en) * 2006-08-09 2008-02-21 The University Of Florida Research Foundation, Inc. Re-engineering bacteria for ethanol production
WO2008120570A1 (ja) * 2007-03-19 2008-10-09 National University Corporation Okayama University タンパク質産生用およびウイルス増殖用の培地

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) * 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters

Also Published As

Publication number Publication date
EP0560885B1 (en) 1999-03-17
AU6194696A (en) 1996-10-31
FI932559A (fi) 1993-07-27
BR9107210A (pt) 1994-02-08
DE69131015D1 (de) 1999-04-22
ATE177784T1 (de) 1999-04-15
JPH06504436A (ja) 1994-05-26
JP2004283176A (ja) 2004-10-14
CA2097803C (en) 2010-08-03
AU694674B2 (en) 1998-07-23
FI111552B (fi) 2003-08-15
DK0560885T3 (da) 1999-10-11
CA2097803A1 (en) 1992-06-08
GR3030570T3 (en) 1999-10-29
FI932559A0 (fi) 1993-06-04
AU9098891A (en) 1992-07-08
AU1858699A (en) 1999-09-09
AU668677B2 (en) 1996-05-16
EP0560885A4 (en) 1994-06-08
EP0560885A1 (en) 1993-09-22
JP3593125B2 (ja) 2004-11-24
WO1992010561A1 (en) 1992-06-25
DE69131015T2 (de) 1999-11-18

Similar Documents

Publication Publication Date Title
ES2132116T3 (es) Celulas recombinantes que expresan altamente genes heterologos cromosomicamente integrados.
FR2279244A1 (fr) Systeme et methode de regulation aptes a limiter la demande d'energie d'une installation industrielle
NZ334032A (en) Strains of Escherichia coli in fermentation processes for L-threonine production
ES8604308A1 (es) Un vector para producir un producto genetico por celulas de insectos
ES474852A1 (es) Un metodo para la expresion,en un huesped bacteriano trans- formado de un polipeptido heterologo con respecto al huespeden su estado no transformado
ATE22302T1 (de) Herstellung von aethylen-terpolymerisaten.
IE831211L (en) Cloning vectors for expression of exogenous protein
AU543124B2 (en) Stage lighting control system
DK268882A (da) Fremgangsmaade til fremstilling af et polypeptid, dna-sekvens, som koder for polypeptidet, replicerbart mikrobielt udtrykkelsesmedium, som i en transformant-mikroorganisme er i stand til at udtrykke polypeptidet og kultur af en mikroorganisme transformeret dermed
WO1992017566A3 (en) Methods for selection of recombinant host cells expressing high levels of a desired protein
DK13182A (da) Fremgangsmaade til kloning af genet der koder for en thermostabil alfa-amylase i escherichia coli og bacillus substilis
FI923758A0 (fi) Expressionssystem.
DK432987D0 (da) Fremgangsmaade til forbedring af ekspressionen af eukaryotprotein
ATE64482T1 (de) Verteilte steuerspeicherarchitektur.
DK336889D0 (da) Polypeptider
ATE173295T1 (de) Ecdysteroid-abhängige gen regulierung in säugetier-zellen
EP0077689A3 (en) Method of gene manipulation using an eukaryotic cell as the host
RU93042475A (ru) Клетка, штаммы, способ получения штамма
ATE106089T1 (de) Nukleare faktoren, die mit der regulierung der transkription assoziiert sind.
IT1120522B (it) Perfezionamento nelle memorie di controllo per sistemi di elaborazione di dati
DE3879534D1 (de) Genetische kontrolleinheit und klonierungs- und expressionssystem.
Miller Symptoms
FR2144084A5 (en) Epoxides - via halohydrins from olefins in high yield - and low cost
JPS57103074A (en) Video generator for digital radar
EP0323838A3 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 560885

Country of ref document: ES