ES2128323T3 - Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas. - Google Patents

Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.

Info

Publication number
ES2128323T3
ES2128323T3 ES91916353T ES91916353T ES2128323T3 ES 2128323 T3 ES2128323 T3 ES 2128323T3 ES 91916353 T ES91916353 T ES 91916353T ES 91916353 T ES91916353 T ES 91916353T ES 2128323 T3 ES2128323 T3 ES 2128323T3
Authority
ES
Spain
Prior art keywords
nucleic acid
pct
reduction
amplification
specific amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91916353T
Other languages
English (en)
Inventor
John J Sninsky
David H Gelfand
Shirley Y Kwok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2128323T3 publication Critical patent/ES2128323T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

LOS METODOS MEJORADOS PARA AMPLIFICAR ACIDOS NUCLEICOS PUEDEN REDUCIR LA AMPLIFICACION NO ESPECIFICA Y MINIMIZAR LOS EFECTOS DE LA CONTAMINACION DE LOS ENSAYOS DE REACCION DE AMPLIFICACION DEL ACIDO NUCLEICO DEBIDA A UN PRODUCTO AMPLIFICADO A PARTIR DE AMPLIFICACIONES PREVIAS. LOS METODOS IMPLICAN LA INTRODUCCION DE BASES NUCELOTIDAS NO CONVENCIONALES EN LOS PRODUCTOS DE REACCION DE AMPLIFICACION Y EL TRATAMIENTO DE LOS PRODUCTOS MEDIANTE MEDIOS ENZIMATICOS (EJ. GLICOSILASAS) Y/O FISICO-QUIMICOS PARA QUE EL PRODUCTO NO PUEDA ACTUAR COMO MODELO EN AMPLIFICACIONES POSTERIORES.
ES91916353T 1990-07-24 1991-07-23 Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas. Expired - Lifetime ES2128323T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55751790A 1990-07-24 1990-07-24
US60915790A 1990-11-02 1990-11-02

Publications (1)

Publication Number Publication Date
ES2128323T3 true ES2128323T3 (es) 1999-05-16

Family

ID=27071456

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91916353T Expired - Lifetime ES2128323T3 (es) 1990-07-24 1991-07-23 Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.

Country Status (11)

Country Link
US (1) US5418149A (es)
EP (1) EP0540693B1 (es)
JP (1) JP3392863B2 (es)
AT (1) ATE176002T1 (es)
AU (1) AU665338B2 (es)
CA (1) CA2087724C (es)
DE (1) DE69130800T2 (es)
DK (1) DK0540693T3 (es)
ES (1) ES2128323T3 (es)
GR (1) GR3029987T3 (es)
WO (1) WO1992001814A2 (es)

Families Citing this family (177)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
CA2159103C (en) 1993-03-26 2002-03-12 Sherrol H. Mcdonough Detection of human immunodeficiency virus type 1
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
ES2161731T3 (es) * 1993-07-01 2001-12-16 Hoffmann La Roche Reactivos y metodos para la transcripcion inversa acoplada a alta temperatura y reaccion en cadena de la polimerasa.
AU8123594A (en) * 1993-10-22 1995-05-08 Abbott Laboratories Reaction tube and method of use to minimize contamination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) * 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
DE4412286A1 (de) * 1994-04-09 1995-10-12 Boehringer Mannheim Gmbh System zur kontaminationsfreien Bearbeitung von Reaktionsabläufen
FR2722799B1 (fr) * 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CA2223050A1 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
AU708821B2 (en) * 1995-07-11 1999-08-12 Forfas Glycosylase mediated detection of nucleotide sequences at candidate loci
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
DE19545320A1 (de) * 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US6436638B1 (en) 1996-05-09 2002-08-20 Metropolitan Water District Of Southern California Cryptosporidium detection method
EP1736554B1 (en) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE19736062A1 (de) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
EP1053348B1 (en) * 1998-02-04 2010-06-02 Life Technologies Corporation Determination of a genotype of an amplification product at multiple allelic sites
WO1999042622A1 (en) * 1998-02-23 1999-08-26 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compound and uses thereof_____________________________________
US6174680B1 (en) 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
JP4196236B2 (ja) * 1998-03-17 2008-12-17 東洋紡績株式会社 核酸増幅用試薬および配列特異的な核酸増幅法
WO1999050447A1 (de) * 1998-03-27 1999-10-07 Mira Diagnostika Gmbh Verfahren und nucleinsäureverbindung zum abbau von in-vitro synthetisierten nucleinsäuremolekülen
CA2266755A1 (en) * 1998-04-24 1999-10-24 James M. Dunn Method for sequencing of nucleic acid polymers
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US7270958B2 (en) * 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
WO2000039345A1 (en) * 1998-12-30 2000-07-06 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP1038974A1 (de) * 1999-03-26 2000-09-27 Mira Diagnostica GmbH Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen
EP1041159A3 (de) * 1999-03-26 2000-10-25 MIRA Diagnostika GmbH Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
WO2001036679A2 (en) * 1999-11-15 2001-05-25 Hartwell John G METHODS FOR GENERATING SINGLE STRANDED cDNA FRAGMENTS
WO2001079548A2 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
EP1307469B1 (en) * 2000-08-03 2008-01-23 Boehringer Mannheim Gmbh Nucleic acid binding compounds containing pyrazolo¬3,4-d pyrimidine analogues of purin-2,6-diamine and their uses
EP1334113A4 (en) * 2000-10-20 2007-08-08 Expression Diagnostics Inc EVALUATION OF LEUCOCYTAIRE EXPRESSION LEVEL
US20020165673A1 (en) * 2001-01-19 2002-11-07 Allen Morgan Method of self-sampling and analyzing a semen sample
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
WO2004011665A2 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
EP2390352A1 (en) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20060263813A1 (en) * 2005-05-11 2006-11-23 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US20070248978A1 (en) * 2006-04-07 2007-10-25 Expression Diagnostics, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20050123956A1 (en) * 2003-09-25 2005-06-09 Affymetrix, Inc. Methods for modifying DNA for microarray analysis
WO2005065321A2 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
EP1718743B1 (en) * 2004-02-04 2009-08-26 QIAGEN North American Holdings, Inc. Anti-freeze protein enhanced nucleic acid amplification
CA2497324A1 (en) * 2004-02-17 2005-08-17 Affymetrix, Inc. Methods for fragmenting and labelling dna
US20050202484A1 (en) 2004-02-19 2005-09-15 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
US20080311627A1 (en) * 2004-06-17 2008-12-18 Epigenomics Ag Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
WO2006034009A2 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
ATE494392T1 (de) 2004-09-21 2011-01-15 Life Technologies Corp Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
NZ555620A (en) * 2004-12-03 2008-08-29 Human Genetic Signatures Pty Methods for simplifying microbial nucleic acids by chemical modification of cytosines
WO2006125267A1 (en) * 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
EP1920064B1 (en) * 2005-07-07 2014-12-24 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
US7435561B2 (en) 2005-07-25 2008-10-14 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
CA2621932A1 (en) * 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for hpv and methylated genomic dna targets to screen for cervical cancer
JP5438320B2 (ja) 2005-10-03 2014-03-12 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 核酸を増幅するための組成物、方法およびキット
US20070087059A1 (en) * 2005-10-17 2007-04-19 Frank Everaerts Bioactive delivery matrix compositions and methods
US8206923B2 (en) 2006-04-24 2012-06-26 Elvira Garza Gonzalez Method for detection and multiple, simultaneous quantification of pathogens by means of real-time polymerase chain reaction
EP2021506B1 (en) 2006-04-28 2016-03-16 Igor Kutyavin Use of products of pcr amplification carrying elements of secondary structure to improve pcr-based nucleic acid detection
JP5878681B2 (ja) 2006-04-28 2016-03-08 イゴール クートヤビン， 核酸の検出を改善するための塩基修飾デオキシヌクレオシド三リン酸の使用
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
WO2008005459A2 (en) * 2006-06-30 2008-01-10 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (en) * 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
FR2914317B1 (fr) * 2007-03-30 2012-12-28 Biomerieux Sa Methode de decontamination d'une solution des acides nucleiques indesirables
US8685675B2 (en) * 2007-11-27 2014-04-01 Human Genetic Signatures Pty. Ltd. Enzymes for amplification and copying bisulphite modified nucleic acids
JP2011505153A (ja) * 2007-12-05 2011-02-24 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Ｒｎａのバイサルファイト処理
EP2071034A1 (en) 2007-12-12 2009-06-17 bioMérieux Method for treating a solution in order to destroy any ribonucleic acid after amplification
WO2009079703A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
US8198023B2 (en) * 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
WO2010048386A1 (en) * 2008-10-24 2010-04-29 Helicos Biosciences Corporation Methods of sample preparation for nucleic acid analysis for nucleic acids available in limited amounts
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
US20120052495A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof
ES2576927T3 (es) 2010-10-22 2016-07-12 T2 Biosystems, Inc. Sistemas de RMN y métodos para la detección rápida de analitos
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
CA2831140C (en) 2011-04-07 2017-11-07 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures
JP5977000B2 (ja) 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
JP5830286B2 (ja) 2011-07-12 2015-12-09 アークレイ株式会社 核酸の増幅検出方法およびキット
WO2013033749A1 (en) 2011-09-07 2013-03-14 Human Genetic Signatures Pty Ltd Molecular detection assay
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
EP2909339B1 (en) 2012-10-18 2018-05-30 IDEXX Laboratories, Inc. Kits comprising controls for nucleic acid amplification
EP3628746A1 (en) 2012-11-02 2020-04-01 Life Technologies Corporation Small rna capture, detection and quantification
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
GB201312995D0 (en) 2013-07-19 2013-09-04 Atlas Genetics Ltd Methods and kits for specific nucleic acid amplification and detection
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
JP6423004B2 (ja) 2014-05-13 2018-11-14 ラシリック, インコーポレイテッドRASIRC, Inc. プロセスガスを重要工程処理に送達するための方法および系
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和***以及用其制备的文库
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016149021A1 (en) 2015-03-13 2016-09-22 Life Technologies Corporation Methods, compositions and kits for small rna capture, detection and quantification
BR112017022329A2 (pt) * 2015-04-17 2018-07-17 Univ California métodos para detectar aglutinação e composições para uso na prática do mesmo
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物***公司 利用有限核苷酸组成的引物扩增
KR101605671B1 (ko) 2015-05-12 2016-03-23 주식회사 엔지노믹스 데옥시유리딘을 포함하는 폴리뉴클레오타이드를 이용한 핵산 증폭반응의 위양성 여부 확인 방법
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3279337A1 (en) 2016-08-04 2018-02-07 Servizo Galego de Saúde (SERGAS) Use of short probes between 8 and 9 nucleotides in multiplex assays
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和***
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10155939B1 (en) * 2017-06-15 2018-12-18 New England Biolabs, Inc. Method for performing multiple enzyme reactions in a single tube
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
MX2018008742A (es) 2018-07-16 2020-01-17 Sigma Alimentos Sa De Cv Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.
US20220389497A1 (en) 2019-11-04 2022-12-08 Mirnax Biosens, S.L. Bivalent reverse primer
EP3901286A1 (en) 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
WO2022232087A1 (en) * 2021-04-30 2022-11-03 Nutcracker Therapeutics, Inc. METHODS FOR PERFORMING IN VITRO TRANSCRIPTION USING dUTP

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4873192A (en) * 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
WO1989011548A1 (en) * 1988-05-20 1989-11-30 Cetus Corporation Immobilized sequence-specific probes
ZA899593B (en) * 1988-12-16 1990-09-26 Siska Diagnostics Inc Self-sustained,sequence replication system
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
ES2080807T3 (es) * 1989-09-01 1996-02-16 Life Technologies Inc Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos.

Also Published As

Publication number Publication date
GR3029987T3 (en) 1999-07-30
WO1992001814A3 (en) 1992-05-14
AU665338B2 (en) 1996-01-04
CA2087724C (en) 2003-09-16
AU8532791A (en) 1992-02-18
CA2087724A1 (en) 1992-01-25
EP0540693A1 (en) 1993-05-12
DE69130800D1 (de) 1999-03-04
US5418149A (en) 1995-05-23
JP3392863B2 (ja) 2003-03-31
EP0540693B1 (en) 1999-01-20
JPH06501612A (ja) 1994-02-24
DE69130800T2 (de) 1999-09-16
WO1992001814A2 (en) 1992-02-06
ATE176002T1 (de) 1999-02-15
DK0540693T3 (da) 1999-09-13

Similar Documents

Publication Publication Date Title
ES2128323T3 (es) Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.
IL81219A0 (en) Method and kit for detecting viruses by amplification and hybridization of nucleic acid sequences and compositions containing such amplified sequences
ATE138108T1 (de) Nachweis des humanen papillomavirus unter verwendung der polymerase-kettenreaktion
IE883131L (en) Nucleic acid multimers and amplified nucleic acid hybridization assays using same
GB2342094B (en) In vitro sorting method
FR2687679B1 (fr) Oligothionucleotides.
PL315803A1 (en) Chemically modified thermostable enzyme, method of obtaining same, method of amplifying nucleic acid, reaction mixture for pcr amplification and pcr set
BR9604814A (pt) Método para amplificar sequências de ácido nucleico especìficas
DE69009774D1 (de) Verfahren zum klonieren und reagenziensatz.
FI894001A0 (fi) Vaextgummimaterial och dess anvaendning i livsmedelsprodukter.
MY131053A (en) 2-saccharinylmethyl aryl carboxylates useful as proteolytic enzyme inhibitors and compositions and method of use thereof
FRÈRE et al. Interaction between the Exocellular DD‐Carboxypeptidase‐Transpeptidase from Streptomyces R61, Substrate and β‐Lactam Antibiotics: A Choice of Models
MY131384A (en) Novel 2-saccharinylmethyl aryl carboxylates useful as proteolytic enzyme inhibitors and compositions and method of use thereof
ATE445021T1 (de) Reduktion von kreuzkontaminationen bei nukleinsäureamplifikationen
JPS51133482A (en) Culture apparatus
JPS51133480A (en) Culture apparatus
NL6912541A (en) Production of uricase useful in treating gout
Rose et al. Studies on a Toxin-Inactivating Substance Excreted by Penicillium cyaneo-fulvum
JPS5598257A (en) Preparing monascus coloring material
DK0635061T3 (da) Fremgangsmåde til begrænsning af genetisk manipulerede organismers overlevelse i deres miljø
FR2306213A1 (fr) Enzymes immobilisees
MX9706609A (es) Procedimiento para la obtencion de aminas opticamente activas.
TW350876B (en) Amplification of nucleic acids and detection of a novel hepatitis G virus (HGV)
JPS5396390A (en) Preparation of 7alpha-methoxycephalosporin derivatives
JPS5571497A (en) Preparation of amoxicillin

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 540693

Country of ref document: ES