DE60322044D1 - Rekombinase-polymerase-amplifikation - Google Patents

Rekombinase-polymerase-amplifikation

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Publication number
DE60322044D1
DE60322044D1 DE60322044T DE60322044T DE60322044D1 DE 60322044 D1 DE60322044 D1 DE 60322044D1 DE 60322044 T DE60322044 T DE 60322044T DE 60322044 T DE60322044 T DE 60322044T DE 60322044 D1 DE60322044 D1 DE 60322044D1
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DE
Germany
Prior art keywords
dna
amplification
polymerase amplification
recombinase polymerase
recombinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE60322044T
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English (en)
Inventor
Niall A Armes
Derek L Stemple
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TwistDix Inc
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TwistDix Inc
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Publication of DE60322044D1 publication Critical patent/DE60322044D1/de
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
    • G06F16/90—Details of database functions independent of the retrieved data types
    • G06F16/95—Retrieval from the web
    • G06F16/951—Indexing; Web crawling techniques
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
    • G06F16/90—Details of database functions independent of the retrieved data types
    • G06F16/95—Retrieval from the web
    • G06F16/957—Browsing optimisation, e.g. caching or content distillation
    • G06F16/9574—Browsing optimisation, e.g. caching or content distillation of access to content, e.g. by caching

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Databases & Information Systems (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Data Mining & Analysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Polyesters Or Polycarbonates (AREA)
DE60322044T 2002-02-21 2003-02-21 Rekombinase-polymerase-amplifikation Expired - Lifetime DE60322044D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35856302P 2002-02-21 2002-02-21
PCT/US2003/005492 WO2003072805A2 (en) 2002-02-21 2003-02-21 Recombinase polymerase amplification

Publications (1)

Publication Number Publication Date
DE60322044D1 true DE60322044D1 (de) 2008-08-21

Family

ID=27765964

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60322044T Expired - Lifetime DE60322044D1 (de) 2002-02-21 2003-02-21 Rekombinase-polymerase-amplifikation

Country Status (11)

Country Link
US (7) US7270981B2 (de)
EP (4) EP2290096B1 (de)
JP (4) JP4340543B2 (de)
AT (1) ATE400663T1 (de)
AU (1) AU2003215391B2 (de)
CA (1) CA2476481C (de)
DE (1) DE60322044D1 (de)
DK (1) DK1499738T3 (de)
ES (1) ES2310237T3 (de)
PT (1) PT1499738E (de)
WO (1) WO2003072805A2 (de)

Families Citing this family (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2305289T3 (es) 2001-04-20 2008-11-01 The Penn State Research Foundation Metodo de manipulacion de acidos nucleicos.
DE60322044D1 (de) * 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CA2498764C (en) * 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US20040241732A1 (en) * 2003-04-11 2004-12-02 Chu-An Chang Method of generating long nucleic acid molecules of defined sequence
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
EP3540073B1 (de) * 2004-06-01 2021-08-25 Abbott Diagnostics Scarborough, Inc. Rekombinase-polymerase-amplifikation
AU2011202896B2 (en) * 2004-06-01 2012-12-06 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
CA2569491A1 (en) 2004-06-02 2005-12-15 Asm Scientific, Inc. 2'-nitrobenzyl-modified ribonucleotides
JP4534138B2 (ja) * 2004-12-21 2010-09-01 アイシン精機株式会社 核酸増幅反応における高度好熱菌由来タンパク質の活性化方法およびその利用
US7579153B2 (en) * 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7435561B2 (en) * 2005-07-25 2008-10-14 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
WO2007087262A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Selective genome amplification
EP2029782B1 (de) * 2006-05-04 2014-11-26 Alere San Diego, Inc. Rekombinase-polymerase-verstärkung
AU2007338862B2 (en) 2006-07-19 2014-02-06 Bionano Genomics, Inc. Nanonozzle device arrays: their preparation and use for macromolecular analysis
EP2136922B1 (de) 2007-03-28 2012-12-05 BioNano Genomics, Inc. Verfahren für makromolekuläre analyse mit nanokanalanordnungen
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
JP5730762B2 (ja) * 2008-06-30 2015-06-10 バイオナノ ジェノミックス、インク． 単一分子全ゲノム解析のための方法及び装置
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
AU2009316628B2 (en) 2008-11-18 2016-06-16 Bionano Genomics, Inc. Polynucleotide mapping and sequencing
SG172965A1 (en) 2009-01-13 2011-08-29 Fluidigm Corp Single-cell nucleic acid analysis
US8685648B2 (en) 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US20100299773A1 (en) * 2009-05-20 2010-11-25 Monsanto Technology Llc Methods and compositions for selecting an improved plant
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2440659B1 (de) 2009-06-10 2016-11-16 Wisconsin Alumni Research Foundation Verfahren zur in-vitro-reparatur von dna-doppelstrang-brüchen und anwendung
NO20092315A (no) * 2009-06-16 2010-12-06 Agr Cannseal As Brønnverktøy og fremgangsmåte for in situ innføring av et behandlingsfluid i et ringrom i en brønn
WO2011047223A1 (en) 2009-10-16 2011-04-21 Monsanto Technology Llc Methods of polynucleotide detection
GB0922097D0 (en) 2009-12-17 2010-02-03 Atlas Genetics Ltd Microbial assay
EP2539464B1 (de) 2010-02-23 2016-11-16 Illumina, Inc. Verfahren zur minimierung sequenzabhängiger amplifikationsunterschiede
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
AU2011312218B2 (en) 2010-10-04 2015-11-05 Sequencing Health, Inc. Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
WO2012051199A2 (en) 2010-10-12 2012-04-19 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event mon87712 and methods for detection thereof
WO2012083137A1 (en) 2010-12-17 2012-06-21 Monsanto Technology Llc Methods for improving competency of plant cells
CA2831140C (en) * 2011-04-07 2017-11-07 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures
WO2012149193A2 (en) 2011-04-29 2012-11-01 Monsanto Technology Llc Diagnostic molecular markers for seed lot purity traits in soybeans
US9476040B2 (en) 2011-05-02 2016-10-25 Board Of Regents Of The University Of Nebraska Plants with useful traits and related methods
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CA2837486C (en) 2011-05-27 2020-10-27 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CA2840630C (en) 2011-06-30 2021-11-30 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof
WO2013012440A2 (en) * 2011-07-21 2013-01-24 Cornell University Methods and devices for dna sequencing and molecular diagnostics
EP2750495B1 (de) 2011-08-31 2019-02-27 Seminis Vegetable Seeds, Inc. Verfahren und zusammensetzungen zur aufrechterhaltung der festigkeit von wassermelonen
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
JP6193252B2 (ja) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド 高効率電子配列決定及び検出のためのシステム並びに方法
JP5958034B2 (ja) * 2012-04-11 2016-07-27 富士レビオ株式会社 分子ビーコン型プローブを用いた標的核酸の検出方法
CN109486902B (zh) 2012-04-19 2023-02-28 生命技术公司 核酸扩增
EP3095879B1 (de) 2012-04-19 2018-09-19 Life Technologies Corporation Nukleinsäureamplifikation
ES2823551T3 (es) 2012-06-08 2021-05-07 Ionian Tech Llc Amplificaciones de ácido nucleico
DE102012014981B3 (de) 2012-07-26 2013-07-18 Sartorius Stedim Biotech Gmbh Verfahren zur Differenzierung zwischen lebenden und toten Zellen
CN107760772B (zh) 2012-08-24 2022-02-01 生命技术公司 用于核酸配对末端测序的方法、组合物、***、仪器和试剂盒
EP2895620B1 (de) 2012-09-11 2017-08-02 Life Technologies Corporation Nukleinsäureamplifikation
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
US10314253B2 (en) 2012-12-04 2019-06-11 Seminis Vegetable Seeds, Inc. Methods and compositions for watermelon sex expression
CA2896879C (en) 2013-03-15 2020-09-22 Genapsys, Inc. Systems and methods for biological analysis
EP2970960B1 (de) 2013-03-15 2019-03-13 Theranos IP Company, LLC Nukleinsäureamplifikation
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US9725760B2 (en) 2013-03-15 2017-08-08 Theranos, Inc. Nucleic acid amplification
US9416387B2 (en) 2013-03-15 2016-08-16 Theranos, Inc. Nucleic acid amplification
US10294489B2 (en) 2013-03-15 2019-05-21 Board Of Trustees Of Southern Illinois University Soybean resistant to cyst nematodes
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
US10059999B2 (en) 2013-06-10 2018-08-28 Monsanto Technology Llc Molecular markers associated with soybean tolerance to low iron growth conditions
US20150132743A1 (en) 2013-09-04 2015-05-14 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
CN103421777B (zh) * 2013-09-11 2015-05-13 北京市农林科学院林业果树研究所 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
EP3502127B1 (de) 2013-10-09 2023-07-26 Monsanto Technology LLC Transgenes ereignis mon87403 in mais und nachweisverfahren dafür
EP3063272A2 (de) 2013-10-30 2016-09-07 Green Life Biotech, LLC Pathogenesequantifizierungssysteme und behandlungsverfahren für mehltau von zitrusgewächsen
NZ735461A (en) 2013-11-27 2020-05-29 Seminis Vegetable Seeds Inc Disease resistance loci in onion
EP3792921A1 (de) 2013-12-11 2021-03-17 Genapsys, Inc. Systeme und verfahren für biologische analysen und berechnungen
WO2015113725A1 (en) 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
BR112016019007B1 (pt) 2014-02-21 2022-05-17 Syngenta Participations Ag Métodos para produzir uma planta de milho, que expressa vip3a ou germoplasma de milho com fertilidade masculina aumentada, programa de melhoramento, método para melhorar a produção de sementes de uma planta de milho que expressa vip3a, e programa de produção de sementes
NZ630710A (en) 2014-02-27 2016-03-31 Seminis Vegetable Seeds Inc Compositions and methods for peronospora resistance in spinach
EP3556864B1 (de) 2014-04-18 2020-12-09 Genapsys, Inc. Verfahren und systeme zur nukleinsäureamplifikation
GB201410022D0 (en) * 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
CN106574305B (zh) 2014-06-13 2021-03-02 生命技术公司 多重核酸扩增
US10316369B2 (en) 2014-06-27 2019-06-11 Seminis Vegetable Seeds, Inc. Methods and assays for male sterile watermelon
EP3169793B1 (de) 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermische verfahren zur amplifizierung von nukleinsäureproben
AU2015323936B2 (en) 2014-09-29 2021-03-11 Illumina Cambridge Limited Recombinase mutants
EP3005862A1 (de) 2014-10-10 2016-04-13 Seminis Vegetable Seeds, Inc. Melonenpflanzen mit verbesserter krankheitstoleranz
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
RU2745987C2 (ru) 2015-04-28 2021-04-05 Монсанто Текнолоджи Ллс Способы и композиции для получения укороченных растений кукурузы
WO2016176358A2 (en) 2015-04-30 2016-11-03 Monsanto Technology Llc Methods for producing canola plants with clubroot resistance and compositions thereof
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、***、组合物、试剂盒、装置和计算机可读媒体
AU2016308049B2 (en) 2015-08-18 2022-03-17 Monsanto Technology Llc Methods for producing cotton plants with enhanced drought tolerance and compositions thereof
US10448595B2 (en) 2015-09-03 2019-10-22 Seminis Vegetable Seeds, Inc. Downy mildew resistant lettuce plants
US10858709B2 (en) 2015-09-10 2020-12-08 Monsanto Technology Llc Methods for producing corn plants with downy mildew resistance and compositions thereof
GB201519565D0 (en) 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
US20180363037A1 (en) 2015-12-09 2018-12-20 Life Technologies Corporation Detection and quantification of nucleic acid molecules associated with a surface
EP3389362A4 (de) 2015-12-18 2019-08-07 Monsanto Technology LLC Verfahren zur herstellung von maispflanzen mit resistenz gegen den nordischen blattbrand und zusammensetzungen davon
US10329601B2 (en) 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
EP3402880B1 (de) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophile dna-polymerase-mutanten
EP3419989A4 (de) 2016-02-26 2019-11-06 Alere San Diego, Inc. Redox-markierte oligonukleotidsonden und deren verwendung
CN109072289A (zh) 2016-03-04 2018-12-21 爱乐圣地亚哥公司 自动化巢式重组酶聚合酶扩增
SG11201807056YA (en) 2016-03-28 2018-10-30 Illumina Inc Recombinase mutants
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
EP3469078B1 (de) 2016-06-10 2024-03-27 Life Technologies Corporation Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的***和方法
WO2018031874A1 (en) 2016-08-11 2018-02-15 Monsanto Technology Llc Methods and compositions for producing corn plants with resistance to late wilt
CN109715646B (zh) 2016-09-15 2023-02-10 雅培实验室 用于样品分析的装置和方法
AU2017232187B2 (en) 2016-09-30 2023-11-09 Seminis Vegetable Seeds, Inc. Xanthomonas resistant brassica oleracea plants
WO2018071522A1 (en) 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
CN106701738B (zh) * 2016-11-10 2020-05-08 中国科学院成都生物研究所 一种等温解开双链dna及制备单链dna的方法
US10632464B2 (en) 2017-02-28 2020-04-28 Alere San Diego, Inc. Microfluidic devices and related methods
WO2018231818A1 (en) 2017-06-16 2018-12-20 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
BR112020004071A2 (pt) 2017-08-31 2020-09-24 Ionian Technologies, Inc. reação de amplificação por nicking e extensão (near) de espécies do vírus sincicial respiratório
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
US11293056B2 (en) 2017-11-07 2022-04-05 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
CN108034763B (zh) * 2017-12-21 2021-10-22 北京卓诚惠生生物科技股份有限公司 检测甲型肝炎和戊型肝炎病毒的引物、探针及试剂盒
US10597714B2 (en) 2017-12-29 2020-03-24 Clear Labs, Inc. Automated priming and library loading device
US10246704B1 (en) 2017-12-29 2019-04-02 Clear Labs, Inc. Detection of microorganisms in food samples and food processing facilities
WO2019133756A1 (en) 2017-12-29 2019-07-04 Clear Labs, Inc. Automated priming and library loading device
US11268102B2 (en) 2018-05-16 2022-03-08 University Of Florida Research Foundation, Incorporated Compositions and methods for identifying and selecting brachytic locus in solanaceae
CN112292460A (zh) 2018-06-12 2021-01-29 主基因有限公司 核酸扩增方法
CN108410954A (zh) * 2018-07-10 2018-08-17 默禾医疗科技（上海）有限公司 一种室温扩增反应体系中反应缓冲液的制配方法
ES2973302T3 (es) 2018-12-14 2024-06-19 Illumina Cambridge Ltd Disminución del ajuste de fase con nucleótidos sin marcar durante la secuenciación
KR20210104555A (ko) 2018-12-17 2021-08-25 일루미나 케임브리지 리미티드 서열분석을 위한 프라이머 올리고뉴클레오타이드
CN112639125A (zh) 2018-12-17 2021-04-09 伊卢米纳剑桥有限公司 用于多核苷酸测序的组合物
CA3145881A1 (en) 2019-01-15 2020-07-23 Seminis Vegetable Seeds, Inc. Green bean plants with improved resistance to sclerotinia sclerotiorum and genetic markers therefor
EP3963103B1 (de) 2019-05-03 2024-02-28 Life Technologies Corporation Verfahren und zusammensetzungen zur manipulation von nukleinsäuren
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
US11905558B2 (en) 2019-08-21 2024-02-20 Life Technologies Corporation System and method for sequencing
SG10202008262UA (en) 2019-09-26 2021-04-29 Seminis Vegetable Seeds Inc Lettuce plants having resistance to nasonovia ribisnigri biotype nr:1
GB201916379D0 (en) 2019-11-11 2019-12-25 Biocrucible Ltd Biochemical reaction methods and reagents
WO2021226754A1 (zh) * 2020-05-09 2021-11-18 杭州梓晶生物有限公司 一种病毒重组酶-聚合酶扩增检测方法
WO2021228839A1 (en) 2020-05-11 2021-11-18 Deutsches Krebsforschungszentrum Polynucleotide detection by cas nucleases
AU2021204717A1 (en) 2020-07-15 2022-02-03 Seminis Vegetable Seeds, Inc. Green Bean Plants with Improved Disease Resistance
US20220170093A1 (en) 2020-11-16 2022-06-02 Life Technologies Corporation System and method for sequencing
US20240182990A1 (en) 2021-03-09 2024-06-06 Etablissement Français Du Sang Detection of infectious agent based on recombinase polymerase amplification combined with a magnetic field-enhanced agglutination
KR20240004692A (ko) 2021-04-29 2024-01-11 애벗트 라보라토리이즈 고-처리량 분석을 위한 샘플 풀링 시스템 및 방법
WO2022266946A1 (zh) * 2021-06-24 2022-12-29 黄婉秋 检测病原体rna、及动物或人类基因rna的试剂盒及其应用
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
WO2023002203A1 (en) 2021-07-21 2023-01-26 Dnae Diagnostics Limited Method and system comprising a cartridge for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
EP4381094A1 (de) 2021-08-03 2024-06-12 Wageningen Universiteit Argonautenbasiertes nukleinsäurenachweissystem
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
CN114237548B (zh) * 2021-11-22 2023-07-18 南京大学 基于非易失性存储器阵列的复数点乘运算的方法及***
CN114085891A (zh) 2021-11-23 2022-02-25 广州达安基因股份有限公司 基于重组酶聚合酶扩增技术的逆转录扩增***和方法
CN113981045A (zh) 2021-11-23 2022-01-28 广州达安基因股份有限公司 制备恒温扩增混合酶系的方法
US20230193310A1 (en) 2021-12-10 2023-06-22 Seminis Vegetabe Seeds, Inc. Lettuce plants having resistance to downy mildew
WO2023250288A2 (en) 2022-06-21 2023-12-28 Seminis Vegetable Seeds, Inc. Novel qtls conferring resistance to cucumber mosaic virus
WO2024030985A1 (en) 2022-08-04 2024-02-08 Abbott Laboratories Assays for detecting monkeypox virus
EP4345444A1 (de) 2022-09-29 2024-04-03 Illumina, Inc. Dynamische kalibrierung eines optischen systems

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US603585A (en) 1898-05-03 cbowell
US5430136A (en) 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5635347A (en) * 1986-04-30 1997-06-03 Igen, Inc. Rapid assays for amplification products
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
GB8903627D0 (en) 1989-02-17 1989-04-05 Unilever Plc Assays
AU635105B2 (en) * 1990-01-26 1993-03-11 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
ATE153707T1 (de) * 1990-05-07 1997-06-15 Daikin Ind Ltd Diagnostische anwendungen der bildung von doppelten d-loops.
DE69130800T2 (de) 1990-07-24 1999-09-16 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5556751A (en) 1991-04-25 1996-09-17 Amoco Corporation Selective amplification system using Q-β replicase
EP0524349A1 (de) * 1991-07-08 1993-01-27 Opticon Sensors Europe B.V. Abtaster mit zwei Brennweiten und die Verwendung eines solchen Abtasters als Lesestift für Kodierungen
EP0543484B1 (de) * 1991-08-30 2001-01-31 Research Development Corporation of Japan Verfahren zur Amplifikation von DNA
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5656430A (en) 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5731150A (en) 1995-11-01 1998-03-24 Chiron Diagnostic Corporation IS6110 based molecular detection of mycobacterium tuberculosis
WO1997046715A1 (en) * 1996-06-03 1997-12-11 New York Society For The Ruptured And Crippled Maintaining The Hospital For Special Surgery Fc receptor polymorphism
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
CA2263815A1 (en) * 1996-08-29 1998-03-05 Koji Kigawa Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase
CA2256563A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
US6245506B1 (en) 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
US7175982B1 (en) 1998-04-22 2007-02-13 Enterprise Ireland (T/A BioResearch Ireland) Method for the characterization of nucleic acid molecules involving generation of extendible upstream DNA fragments resulting from the cleavage of nucleic acid at an abasic site
WO1999060158A1 (fr) 1998-05-19 1999-11-25 Laboratory Of Molecular Biophotonics Phase solide utilisee pour detecter de l'acide nucleique et procede de detection d'acide nucleique
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
CA2375769A1 (en) 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
US6699693B1 (en) * 1999-02-04 2004-03-02 Sloan-Kettering Institute For Cancer Research Process for DNA replication
WO2000046408A1 (en) 1999-02-04 2000-08-10 Sloan-Kettering Institute For Cancer Research Process for dna replication
US6387621B1 (en) 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
NO314091B1 (no) * 2000-01-12 2003-01-27 Biotec Pharmacon Asa Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
AU2001243670A1 (en) 2000-03-20 2001-10-03 Maxygen, Inc. Method for generating recombinant dna molecules in complex mixtures
DE60143974D1 (de) 2000-03-29 2011-03-10 Lgc Ltd Hybridisierungsprobe und Methode zum schnellen Nachweis und zur schnellen Unterscheidung von Sequenzen
US20020061530A1 (en) 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
US6379899B1 (en) * 2001-03-13 2002-04-30 Discoverx Isothermal exponential RNA amplification in complex mixtures
ES2305289T3 (es) 2001-04-20 2008-11-01 The Penn State Research Foundation Metodo de manipulacion de acidos nucleicos.
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
CA2492220C (en) 2001-07-15 2014-03-18 Keck Graduate Institute Nucleic acid amplification using nicking agents
EP1417336A4 (de) 2001-07-15 2005-06-22 Keck Graduate Inst Exponentielle nukleinsäureamplifikation unter verwendung von nicking-endonukleasen
KR20040028991A (ko) 2001-08-20 2004-04-03 다카라 바이오 가부시키가이샤 핵산 증폭 방법
AU2002341898A2 (en) 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
KR100445560B1 (ko) 2001-10-31 2004-08-21 (주)바이오넥스 핵산 또는 생물학적 물질을 분리하기 위한 키트의 제조방법과, 그 방법에 의해 제조된 키트와, 그 키트를사용하는 장치
US7244562B2 (en) * 2001-11-01 2007-07-17 Gene Check, Inc. RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US20040137456A1 (en) * 2002-04-04 2004-07-15 Hiroki Yokota Method for identifying and characterizing individual dna molecules
US20030228611A1 (en) 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US6713262B2 (en) 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
JP2005532827A (ja) 2002-07-12 2005-11-04 ブリティッシュ・バイオセル・インターナショナル・リミテッド 側方フローアッセイ用のデバイスおよびその方法
US20040038213A1 (en) 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
AU2003274914A1 (en) 2002-08-21 2004-03-11 Epoch Biosciences, Inc. Abasic site endonuclease assay
CA2498764C (en) * 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
WO2004081224A2 (en) * 2003-03-11 2004-09-23 Gene Check, Inc. Reca-assisted allele specific oligonucleotide extension method
DE10315640A1 (de) 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
JP2005110621A (ja) * 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
CA2497324A1 (en) 2004-02-17 2005-08-17 Affymetrix, Inc. Methods for fragmenting and labelling dna
EP3540073B1 (de) 2004-06-01 2021-08-25 Abbott Diagnostics Scarborough, Inc. Rekombinase-polymerase-amplifikation
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
DK1659186T3 (da) 2004-10-11 2008-09-22 Epigenomics Ag Fremgangsmåde til kontaminationsbeskyttelse i DNA-amplifikationssystemer til methyleringsanalyse opnået ved en modificeret forbehandling af nukleinsyrer
WO2006057918A2 (en) 2004-11-23 2006-06-01 Morisawa, Shinkatsu Detection of nucleic acid variation by cleavage-amplification method
WO2006119280A2 (en) 2005-05-03 2006-11-09 Handylab, Inc. Lyophilized pellets
US20070042427A1 (en) 2005-05-03 2007-02-22 Micronics, Inc. Microfluidic laminar flow detection strip
WO2006125267A1 (en) 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
WO2007008997A2 (en) 2005-07-12 2007-01-18 The Board Of Trustees Of The Leland Stanford Junior University Template specific inhibition of pcr
US7435561B2 (en) 2005-07-25 2008-10-14 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
EP2029782B1 (de) * 2006-05-04 2014-11-26 Alere San Diego, Inc. Rekombinase-polymerase-verstärkung
WO2009011971A2 (en) 2007-05-18 2009-01-22 The Govt. Of The U.S.A. As Represented By The Secretary Of The Dept. Of Health And Human Services, Centers For Disease Control And Prevention Primers and probes for the detection of streptococcus pneumoniae
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
ES2320840B1 (es) 2007-11-27 2010-07-01 Nelson Alfonso Egued Tubo humectador de doble camara.
JP2009139487A (ja) 2007-12-04 2009-06-25 Nippon Sheet Glass Co Ltd 正立等倍レンズアレイプレート
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
ES2823551T3 (es) 2012-06-08 2021-05-07 Ionian Tech Llc Amplificaciones de ácido nucleico

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