ES2119756T3 - Genes de beta lactama acilasa mutados. - Google Patents

Genes de beta lactama acilasa mutados.

Info

Publication number
ES2119756T3
ES2119756T3 ES91200930T ES91200930T ES2119756T3 ES 2119756 T3 ES2119756 T3 ES 2119756T3 ES 91200930 T ES91200930 T ES 91200930T ES 91200930 T ES91200930 T ES 91200930T ES 2119756 T3 ES2119756 T3 ES 2119756T3
Authority
ES
Spain
Prior art keywords
beta
acilasa
lactama
lactam
mutated genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91200930T
Other languages
English (en)
Inventor
Wilhelmus Johannes Dr Quax
Der Laan Jan Metske Dr Van
Onno Dr Misset
Herman B M Dr Lenting
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of ES2119756T3 publication Critical patent/ES2119756T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01011—Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pyrrole Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PROPORCIONAN NUEVOS MUTANTES DE ACILASA BETA-LACTAN QUE EXHIBEN ESPECIFICACIONES DE SUSTRATO ALTERADAS. ESTAS ACILASAS BETA-LACTAM SON OBTENIDAS MEDIANTE LA EXPRESION DE UN GEN CODIFICANDO DICHA ACILASA BETA-LACTAM Y TENIENDO UNA SECUENCIA AMINO ACIDA QUE DEFIERE AL MENOS EN UN AMINO ACIDO DEL TIPO SALVAJE DE ACILASA BETA-LACTAM.
ES91200930T 1990-04-18 1991-04-18 Genes de beta lactama acilasa mutados. Expired - Lifetime ES2119756T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP90200962 1990-04-18

Publications (1)

Publication Number Publication Date
ES2119756T3 true ES2119756T3 (es) 1998-10-16

Family

ID=8204994

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91200930T Expired - Lifetime ES2119756T3 (es) 1990-04-18 1991-04-18 Genes de beta lactama acilasa mutados.

Country Status (10)

Country Link
US (2) US5457032A (es)
EP (1) EP0453048B1 (es)
JP (1) JPH05501806A (es)
KR (1) KR100193802B1 (es)
AT (1) ATE167520T1 (es)
DE (1) DE69129604T2 (es)
ES (1) ES2119756T3 (es)
IE (1) IE911295A1 (es)
PT (1) PT97409B (es)
WO (1) WO1991016435A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05501806A (ja) * 1990-04-18 1993-04-08 ギスト　ブロカデス　ナムローゼ　フェンノートシャップ 変異β―ラクタムアシラーゼ遺伝子
PT638649E (pt) * 1993-08-05 2000-04-28 Dsm Nv Nova utilizacao de penicilina g acilase de alcaligenes faecalis
DE69514923T2 (de) * 1994-08-12 2000-11-02 Dsm Nv Mutierte penicillin-g-acylase gene
ATE402997T1 (de) * 1996-11-05 2008-08-15 Bristol Myers Squibb Co Mutierte penicillin g acylasen
DK1276855T3 (da) * 2000-04-17 2012-11-26 Dyax Corp Fremgangsmåde til konstruktion af visningsbiblioteker over genetiske pakker for medlemmer af en diversificeret peptidfamilie
US8288322B2 (en) 2000-04-17 2012-10-16 Dyax Corp. Methods of constructing libraries comprising displayed and/or expressed members of a diverse family of peptides, polypeptides or proteins and the novel libraries
EP1360288B1 (en) 2000-12-18 2011-02-16 Dyax Corp. Focused libraries of genetic packages
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
WO2004111241A1 (en) * 2003-06-12 2004-12-23 Stichting Voor De Technische Wetenschappen Penicillin acylase
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
EP1538205A1 (en) * 2003-12-05 2005-06-08 Stichting Voor De Technische Wetenschappen Glutaryl amidases and their uses
WO2005054452A2 (en) * 2003-12-05 2005-06-16 Stichting Voor De Technische Wetenschappen Glutaryl amidases and their uses
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
ES2571865T3 (es) 2005-12-28 2016-05-27 Dsm Sinochem Pharm Nl Bv Acilasas de beta-lactama de tipo II mutantes
CA2697193C (en) 2007-09-14 2017-06-06 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
WO2009114815A1 (en) 2008-03-13 2009-09-17 Dyax Corp Libraries of genetic packages comprising novel hc cdr3 designs
EP2281078B1 (en) 2008-04-24 2014-10-22 Dyax Corporation Libraries of genetic packages comprising novel hc cdr1, cdr2, and cdr3 and novel lc cdr1, cdr2, and cdr3 designs
WO2010054319A2 (en) * 2008-11-10 2010-05-14 Codexis, Inc. Penicillin-g acylases
CA2773564A1 (en) * 2009-09-14 2011-03-17 Dyax Corp. Libraries of genetic packages comprising novel hc cdr3 designs
AU2011279073B2 (en) 2010-07-16 2016-06-09 Adimab, Llc Antibody libraries
CA2894595A1 (en) 2012-12-14 2014-06-19 Saban Ventures Pty Limited Synergistic disinfection enhancement
EP2934132B1 (en) 2012-12-14 2018-07-04 Saban Ventures Pty Limited Disinfectant
CN111172142B (zh) * 2020-02-14 2021-09-28 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体
CN116144517A (zh) * 2021-11-22 2023-05-23 中国科学院天津工业生物技术研究所 一种7-氨基头孢烷酸生产菌及其制备方法与应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6121097A (ja) * 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
US4981789A (en) * 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (en) * 1987-12-21 1991-01-30 Merck & Co. Inc. One-step enzymatic conversion of cephalosporin c and derivatives to 7-aminocephalosporanic acid and derivatives
DE69110952T2 (de) * 1990-04-18 1995-11-16 Gist Brocades Nv Penicillin-G-Acylase, dafür kodierendes Gen und Verfahren zur Herstellung von diesem Enzym.
JPH05501806A (ja) * 1990-04-18 1993-04-08 ギスト　ブロカデス　ナムローゼ　フェンノートシャップ 変異β―ラクタムアシラーゼ遺伝子
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) * 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) * 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase

Also Published As

Publication number Publication date
IE911295A1 (en) 1991-10-23
JPH05501806A (ja) 1993-04-08
EP0453048A1 (en) 1991-10-23
EP0453048B1 (en) 1998-06-17
US5457032A (en) 1995-10-10
PT97409B (pt) 1998-08-31
US5935831A (en) 1999-08-10
ATE167520T1 (de) 1998-07-15
DE69129604D1 (de) 1998-07-23
KR100193802B1 (ko) 1999-06-15
DE69129604T2 (de) 1998-10-15
PT97409A (pt) 1992-01-31
WO1991016435A1 (en) 1991-10-31

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