ES2108719T3 - Inmunoensayo de analitos libres. - Google Patents

Inmunoensayo de analitos libres.

Info

Publication number
ES2108719T3
ES2108719T3 ES92111702T ES92111702T ES2108719T3 ES 2108719 T3 ES2108719 T3 ES 2108719T3 ES 92111702 T ES92111702 T ES 92111702T ES 92111702 T ES92111702 T ES 92111702T ES 2108719 T3 ES2108719 T3 ES 2108719T3
Authority
ES
Spain
Prior art keywords
analyte
sample
free
mixture
receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92111702T
Other languages
English (en)
Inventor
Moshe Schwarzberg
Santosh Dayal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of ES2108719T3 publication Critical patent/ES2108719T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

UN METODO PARA DETERMINAR EL NIVEL DE MATERIALES DE ANALISIS LIBRES DE UNA MUESTRA QUE CONTIENE EL MATERIAL DE ANALISIS EN PORCIONES LIBRES Y ENLAZADAS QUE SE LLEVA A CABO MEDIANTE LOS PASOS SECUENCIALES QUE SE INDICAN A CONTINUACION. EL MUESTRA SE COMBINA CON UN PRIMER VOLUMEN DE LIQUIDO CONOCIDO QUE CONTENGA UN ANTIRRECEPTOR DE LOS MATERIALES DE ANALISIS SOLUBLE EN EXCESO RESPECTO AL MATERIAL DE ANALISIS LIBRE QUE SE ENCUENTRA EN LA MUESTRA. LA MEZCLA RESULTANTE SE INCUBA DURANTE UN PERIODO DE TIEMPO SUFICIENTE COMO PARA PERMITIR LA REACCION DE ENLACE DEL MATERIAL DE ANALISIS LIBRE CON EL ANTIRRECEPTOR DE LOS MATERIALES DE ANALISIS PARA CONSEGUIR UNA TERMINACION SUSTANCIAL PARA FORMAR UNA PRIMERA MEZCLA DE INCUBACION. DESPUES SE AÑADE UN SEGUNDO VOLUMEN DE LIQUIDO CONOCIDO QUE CONTENGA UN CONJUGADO SOLUBLE SUFICIENTE COMO PARA REDUCIR LA PROPORCION DE LA MUESTRA EN LA MEZCLA RESULTANTE A UN MAS DEL 10% V/V, CONSTANDO EL CONJUGADO DE UN MATERIAL DE ANALISIS MARCADO O UN ANALOGO MARCADO DELMISMO, PARA PERMITIR QUE EL CONJUGADO SE UNA AL ANTIRRECEPTOR DE LOS MATERIALES DE ANALISIS QUE NO SE HA ENLAZADO EN LA PRIMERA MEZCLA DE INCUBACION PARA FORMAR UNA SEGUNDA MEZCLA DE INCUBACION. DESPUES SE INTRODUCE UN ELEMENTO DE SEPARACION EN LA SEGUNDA MEZCLA DE INCUBACION QUE CONTENGA UNA FASE SOLIDA REVESTIDA CON UN AGLUTINANTE INMOVILIZADO PARA EL ANTIRRECEPTOR DE LOS MATERIALES DE ANALISIS, CUYO AGLUTINANTE SE ENCUENTRA EN EXCESO RESPECTO AL ANTIRRECEPTOR DE LOS MATERIALES DE ANALISIS PARA ENLAZARSE SUSTANCIALMENTE A TODOS LOS ANTIRRECEPTORES DE LOS MATERIALES DE ANALISIS DE LA MEZCLA DE INCUBACION. LA FASE SOLIDA SE SEPARA ENTONCES Y SE LAVA DE LA MEZCLA. SE MIDE LA CANTIDAD DE CONJUGADO ENLAZADO A LA FASE SOLIDA Y SE DETERMINA LA CONCENTRACION DE MATERIALES DE ANALISIS LIBRES QUE HAY EN LA MUESTRA.
ES92111702T 1991-07-22 1992-07-09 Inmunoensayo de analitos libres. Expired - Lifetime ES2108719T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/733,934 US5464749A (en) 1991-07-22 1991-07-22 Immunoassay of free substances in biological fluids

Publications (1)

Publication Number Publication Date
ES2108719T3 true ES2108719T3 (es) 1998-01-01

Family

ID=24949687

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92111702T Expired - Lifetime ES2108719T3 (es) 1991-07-22 1992-07-09 Inmunoensayo de analitos libres.

Country Status (9)

Country Link
US (1) US5464749A (es)
EP (1) EP0524502B1 (es)
JP (1) JP3348250B2 (es)
AT (1) ATE160018T1 (es)
AU (1) AU654557B2 (es)
CA (1) CA2069554C (es)
DE (1) DE69222991T2 (es)
ES (1) ES2108719T3 (es)
IL (1) IL101921A0 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561049A (en) * 1994-09-21 1996-10-01 Behringwerke Ag Method for detecting antibodies
US5786153A (en) * 1996-09-12 1998-07-28 Chiron Diagnostics Corporation Prevention of probe coating on automated analyzers using a non-denaturing surfactant
EP1072887B1 (en) * 1999-07-30 2005-11-16 Mitsubishi Chemical Corporation Immunoassay
DE60233060D1 (de) 2001-09-06 2009-09-03 Genomic Profiling Systems Inc Schnellnachweis replizierender zellen
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
CN102239245A (zh) 2008-09-24 2011-11-09 施特劳斯控股公司 用于检测分析物的方法
WO2011057347A1 (en) 2009-11-12 2011-05-19 Tgr Biosciences Pty Ltd Analyte detection
PL3327110T3 (pl) 2011-11-07 2020-07-27 Rapid Micro Biosystems, Inc. Kaseta do badania sterylności
WO2013158666A1 (en) 2012-04-16 2013-10-24 Rapid Micro Biosystems, Inc. Cell culturing device
WO2014039591A1 (en) * 2012-09-04 2014-03-13 Mamenta Edward L System and method for spatiotemporally analyzed rapid assays
US12031985B2 (en) 2018-04-19 2024-07-09 First Light Diagnostics, Inc. Detection of targets
CN110717515A (zh) * 2019-09-06 2020-01-21 北京三快在线科技有限公司 模型训练方法、装置及电子设备

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US4040907A (en) 1974-06-20 1977-08-09 Syva Company Iodothyronine enzyme conjugates
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4292296A (en) * 1978-09-12 1981-09-29 Baxter Travenol Laboratories, Inc. Diagnostic method
US4366143A (en) * 1979-09-24 1982-12-28 Amersham International Public Limited Company Assay for the free portion of substances in biological fluids
US4391795A (en) 1980-03-21 1983-07-05 Becton Dickinson And Company Assay for free thyroid hormone
US4410633A (en) 1980-09-25 1983-10-18 Corning Glass Works Method for the measurement of free thyroxine or 3,5,3'-triiodothyronine in a liquid sample
EP0089806B1 (en) * 1982-03-22 1989-06-28 AMERSHAM INTERNATIONAL plc Assay for the free portion of substances in biological fluids
IL94212A0 (en) 1989-07-24 1991-01-31 Tri Tech Partners And Triton B Automated analytical apparatus and method

Also Published As

Publication number Publication date
DE69222991T2 (de) 1998-03-05
JPH05203646A (ja) 1993-08-10
JP3348250B2 (ja) 2002-11-20
IL101921A0 (en) 1992-12-30
ATE160018T1 (de) 1997-11-15
AU654557B2 (en) 1994-11-10
EP0524502A2 (en) 1993-01-27
EP0524502B1 (en) 1997-11-05
CA2069554C (en) 1999-12-28
AU1815592A (en) 1993-01-28
EP0524502A3 (en) 1993-07-14
US5464749A (en) 1995-11-07
DE69222991D1 (de) 1997-12-11
CA2069554A1 (en) 1993-01-23

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