ES2062253T3 - Metodo para solubilizacion y naturalizacion de somatotropinas utilizando una baja concentracion de urea. - Google Patents

Metodo para solubilizacion y naturalizacion de somatotropinas utilizando una baja concentracion de urea.

Info

Publication number
ES2062253T3
ES2062253T3 ES90120309T ES90120309T ES2062253T3 ES 2062253 T3 ES2062253 T3 ES 2062253T3 ES 90120309 T ES90120309 T ES 90120309T ES 90120309 T ES90120309 T ES 90120309T ES 2062253 T3 ES2062253 T3 ES 2062253T3
Authority
ES
Spain
Prior art keywords
somatotropins
solubilization
naturalization
urea
low concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90120309T
Other languages
English (en)
Inventor
Kevin Michael Mccoy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth Holdings LLC
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Application granted granted Critical
Publication of ES2062253T3 publication Critical patent/ES2062253T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN PROCESO PARA LA SOLUBILIZACION Y NATURACION DE SOMATROPINAS UTILIZANDO UNA COMBINACION DE UNA BAJA DISOLUCION DE UREA Y UNA DISOLUCION ACUOSA ALCALINA. UTILIZANDO EL PROCESO DE LA PRESENTE INVENCION, SE OBTIENEN MEJORES RENDIMIENTOS DEL PRODUCTO FINAL.
ES90120309T 1989-12-05 1990-10-23 Metodo para solubilizacion y naturalizacion de somatotropinas utilizando una baja concentracion de urea. Expired - Lifetime ES2062253T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44628089A 1989-12-05 1989-12-05

Publications (1)

Publication Number Publication Date
ES2062253T3 true ES2062253T3 (es) 1994-12-16

Family

ID=23772005

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90120309T Expired - Lifetime ES2062253T3 (es) 1989-12-05 1990-10-23 Metodo para solubilizacion y naturalizacion de somatotropinas utilizando una baja concentracion de urea.

Country Status (16)

Country Link
EP (1) EP0432419B1 (es)
JP (1) JPH03181500A (es)
KR (1) KR0160975B1 (es)
AT (1) ATE113060T1 (es)
AU (1) AU628695B2 (es)
CA (1) CA2031369A1 (es)
DE (1) DE69013475T2 (es)
DK (1) DK0432419T3 (es)
ES (1) ES2062253T3 (es)
FI (1) FI97296C (es)
HU (1) HU214249B (es)
IE (1) IE66142B1 (es)
IL (1) IL96124A (es)
NZ (1) NZ236141A (es)
PT (1) PT96058B (es)
ZA (1) ZA909745B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994018227A2 (en) * 1993-02-04 1994-08-18 Denzyme Aps Improved method for the refolding of proteins
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
US6632425B1 (en) 1997-03-20 2003-10-14 Human Genome Sciences, Inc. Chemokine compositions
AU739394B2 (en) * 1998-08-27 2001-10-11 Lg Chemical Limited Process for the preparation of active somatotropin from inclusion bodies
DE10044360A1 (de) * 2000-09-08 2002-03-21 Merck Patent Gmbh Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen
US20040116663A1 (en) * 2001-01-16 2004-06-17 Soren Buus Method for refolding of proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
AU612133B2 (en) * 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
JPH01257491A (ja) * 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法

Also Published As

Publication number Publication date
FI905985A (fi) 1991-06-06
PT96058B (pt) 1998-07-31
EP0432419B1 (en) 1994-10-19
IL96124A (en) 1998-06-15
IE66142B1 (en) 1995-12-13
ZA909745B (en) 1991-10-30
CA2031369A1 (en) 1991-06-06
HU908077D0 (en) 1991-06-28
FI97296B (fi) 1996-08-15
HU214249B (hu) 1998-03-02
NZ236141A (en) 1993-01-27
FI97296C (fi) 1996-11-25
IL96124A0 (en) 1991-07-18
KR910012235A (ko) 1991-08-07
IE904204A1 (en) 1991-06-05
DK0432419T3 (da) 1994-11-14
KR0160975B1 (ko) 1998-11-16
AU6773690A (en) 1991-06-13
HUT58762A (en) 1992-03-30
EP0432419A1 (en) 1991-06-19
FI905985A0 (fi) 1990-12-04
PT96058A (pt) 1991-09-30
DE69013475T2 (de) 1995-05-04
AU628695B2 (en) 1992-09-17
ATE113060T1 (de) 1994-11-15
DE69013475D1 (de) 1994-11-24
JPH03181500A (ja) 1991-08-07

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