ES2055661A1 - Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. - Google Patents
Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre.Info
- Publication number
- ES2055661A1 ES2055661A1 ES09300103A ES9300103A ES2055661A1 ES 2055661 A1 ES2055661 A1 ES 2055661A1 ES 09300103 A ES09300103 A ES 09300103A ES 9300103 A ES9300103 A ES 9300103A ES 2055661 A1 ES2055661 A1 ES 2055661A1
- Authority
- ES
- Spain
- Prior art keywords
- rna
- free
- capillary electrophoresis
- determination
- gene expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
DETERMINACION DE LA EXPRESION GENICA POR CAPTURA ESPECIFICA DE ARN Y SU CUANTIFICACION DIRECTA POR ELECTROFORESIS CAPILAR EN ZONA LIBRE. METODO DE DETERMINACION DE LA EXPRESION GENICA POR DETECCION DIRECTA DE TRANSCRITOS MEDIANTE ELECTROFORESIS CAPILAR EN ZONA LIBRE PARA MUESTRAS BIOLOGICAS. MUESTRAS DE ARN PURIFICADAS O SEMIPURIFICADAS SE HIBRIDAN CON UN OLIGONUCLEOTIDO O SONDA ESPECIFICA PREVIAMENTE SELECCIONADA, MARCADA CON BIOTINA Y PERTENECIENTE A UNA SECUENCIA CODIFICANTE DE UNA GEN. A LA SOLUCION DE HIBRIDACION CONTENIENDO EL HIBRIDOESPECIFICO SE LE AÑADEN ESFERAS MAGNETICAS CARGADAS CON RSTREPTAVIDINA. SE APLICA SEGUIDAMENTE UN SISTEMA MAGNETICO QUE SELECCIONA EN LA PARED DEL TUBO EL COMPLEJO ESTEPTAVIDINA-BIOTINA-OLIGONUCLEOTIDO-O-SONDA RNA ESPECIFICO, SE LAVA EL COMPLEJO CON SOLUCIONES ASTRINGENTES Y A CONTINUACION SE ELUYE EL RNA ESPECIFICO. EL RNA OBTENIDO SE INYECTA EN UN SISTEMA DE ELECTROFORESIS CAPILAR EN ZONA LIBRE Y LA SEÑAL OBTENIDA POR EL DETECTOR PERMITE LA CUANTIFICACION DIRECTA DE LA MUESTRA.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09300103A ES2055661B1 (es) | 1993-01-20 | 1993-01-20 | Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09300103A ES2055661B1 (es) | 1993-01-20 | 1993-01-20 | Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2055661A1 true ES2055661A1 (es) | 1994-08-16 |
ES2055661B1 ES2055661B1 (es) | 1995-03-01 |
Family
ID=8280520
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09300103A Expired - Fee Related ES2055661B1 (es) | 1993-01-20 | 1993-01-20 | Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2055661B1 (es) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2187283A (en) * | 1986-02-27 | 1987-09-03 | Orion Yhtymae Oy | Quantification of nucleic acid molecules |
EP0304845A2 (en) * | 1987-08-28 | 1989-03-01 | Profile Diagnostic Sciences Inc. | Method and kit for assaying gene expressions |
WO1990006045A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Nucleic acid probes |
WO1990006042A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Detection and quantitative determination of rna and dna |
WO1991002244A1 (en) * | 1989-08-07 | 1991-02-21 | Applied Biosystems, Inc. | Nucleic acid fractionation by counter-migration capillary electrophoresis |
-
1993
- 1993-01-20 ES ES09300103A patent/ES2055661B1/es not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2187283A (en) * | 1986-02-27 | 1987-09-03 | Orion Yhtymae Oy | Quantification of nucleic acid molecules |
EP0304845A2 (en) * | 1987-08-28 | 1989-03-01 | Profile Diagnostic Sciences Inc. | Method and kit for assaying gene expressions |
WO1990006045A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Nucleic acid probes |
WO1990006042A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Detection and quantitative determination of rna and dna |
WO1991002244A1 (en) * | 1989-08-07 | 1991-02-21 | Applied Biosystems, Inc. | Nucleic acid fractionation by counter-migration capillary electrophoresis |
Also Published As
Publication number | Publication date |
---|---|
ES2055661B1 (es) | 1995-03-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20141120 |