ES2055661A1 - Determination of gene expression by specific capture of RNA and direct quantification thereof by free-zone capillary electrophoresis - Google Patents
Determination of gene expression by specific capture of RNA and direct quantification thereof by free-zone capillary electrophoresisInfo
- Publication number
- ES2055661A1 ES2055661A1 ES09300103A ES9300103A ES2055661A1 ES 2055661 A1 ES2055661 A1 ES 2055661A1 ES 09300103 A ES09300103 A ES 09300103A ES 9300103 A ES9300103 A ES 9300103A ES 2055661 A1 ES2055661 A1 ES 2055661A1
- Authority
- ES
- Spain
- Prior art keywords
- rna
- free
- capillary electrophoresis
- determination
- gene expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Determination of gene expression by specific capture of RNA and direct quantification thereof by free-zone capillary electrophoresis. Method for determination of gene expression by direct detection of transcripts by means of free-zone capillary electrophoresis for biological samples. Purified or semi-purified RNA samples are hybridized with an oligonucleotide or previously selected specific probe, labelled with biotin and belonging to a gene coding sequence. Magnetic spheres loaded with streptavidin are added to the hybridization solution containing the specific hybrid. A magnetic system is then applied which selects the streptavidin- biotin-oligonucleotide or specific RNA probe complex in the wall of the tube, the complex is washed with astringent solutions and the specific RNA is then eluted. The RNA obtained is injected into a free-zone capillary electrophoresis system and the signal obtained by the detector allows direct quantification of the sample.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09300103A ES2055661B1 (en) | 1993-01-20 | 1993-01-20 | DETERMINATION OF THE GENE EXPRESSION BY SPECIFIC CAPTURE OF RNA AND ITS DIRECT QUANTIFICATION BY CAPILLARY ELECTROPHORESIS IN A FREE ZONE. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09300103A ES2055661B1 (en) | 1993-01-20 | 1993-01-20 | DETERMINATION OF THE GENE EXPRESSION BY SPECIFIC CAPTURE OF RNA AND ITS DIRECT QUANTIFICATION BY CAPILLARY ELECTROPHORESIS IN A FREE ZONE. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2055661A1 true ES2055661A1 (en) | 1994-08-16 |
ES2055661B1 ES2055661B1 (en) | 1995-03-01 |
Family
ID=8280520
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09300103A Expired - Fee Related ES2055661B1 (en) | 1993-01-20 | 1993-01-20 | DETERMINATION OF THE GENE EXPRESSION BY SPECIFIC CAPTURE OF RNA AND ITS DIRECT QUANTIFICATION BY CAPILLARY ELECTROPHORESIS IN A FREE ZONE. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2055661B1 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2187283A (en) * | 1986-02-27 | 1987-09-03 | Orion Yhtymae Oy | Quantification of nucleic acid molecules |
EP0304845A2 (en) * | 1987-08-28 | 1989-03-01 | Profile Diagnostic Sciences Inc. | Method and kit for assaying gene expressions |
WO1990006045A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Nucleic acid probes |
WO1990006042A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Detection and quantitative determination of rna and dna |
WO1991002244A1 (en) * | 1989-08-07 | 1991-02-21 | Applied Biosystems, Inc. | Nucleic acid fractionation by counter-migration capillary electrophoresis |
-
1993
- 1993-01-20 ES ES09300103A patent/ES2055661B1/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2187283A (en) * | 1986-02-27 | 1987-09-03 | Orion Yhtymae Oy | Quantification of nucleic acid molecules |
EP0304845A2 (en) * | 1987-08-28 | 1989-03-01 | Profile Diagnostic Sciences Inc. | Method and kit for assaying gene expressions |
WO1990006045A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Nucleic acid probes |
WO1990006042A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Detection and quantitative determination of rna and dna |
WO1991002244A1 (en) * | 1989-08-07 | 1991-02-21 | Applied Biosystems, Inc. | Nucleic acid fractionation by counter-migration capillary electrophoresis |
Also Published As
Publication number | Publication date |
---|---|
ES2055661B1 (en) | 1995-03-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20141120 |