ES2010763A6 - Un metodo para producir una nitrilasa especifica capaz de hidrolizar un p-hidroxibenzonitrilo dihalogenado en 3,5. - Google Patents

Un metodo para producir una nitrilasa especifica capaz de hidrolizar un p-hidroxibenzonitrilo dihalogenado en 3,5.

Info

Publication number
ES2010763A6
ES2010763A6 ES8802134A ES8802134A ES2010763A6 ES 2010763 A6 ES2010763 A6 ES 2010763A6 ES 8802134 A ES8802134 A ES 8802134A ES 8802134 A ES8802134 A ES 8802134A ES 2010763 A6 ES2010763 A6 ES 2010763A6
Authority
ES
Spain
Prior art keywords
gene
bromoxynil
haloarylnitrile
cells containing
nitrilase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8802134A
Other languages
English (en)
Inventor
David Stalker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience SA
Original Assignee
Rhone Poulenc Agrochimie SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Agrochimie SA filed Critical Rhone Poulenc Agrochimie SA
Publication of ES2010763A6 publication Critical patent/ES2010763A6/es
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

METODO PARA PRODUCIR UNA NITRILASA ESPECIFICA CAPAZ DE HIDROLIZAR UN P-HIDROXIBENZONITRILO DIHALOGENADO EN 3,5. CONSISTE EN SELECCIONAR KLEBSIELLA PNEUMONIAE, SUBESPECIE OZAENAE, ESCINDIR EL GENOMA DE DICHA BACTERIA, PRODUCIENDO FRAGMENTOS DE ADN, CLONAR ESTOS FRAGMENTOS MEDIANTE VECTORES EN ESCHERICHIA COLI, SELECCIONAR UNA NITRILASA BACTERIANA PURA, AISLAR LA SECUENCIA DE ADN CON EL GEN ESTRUCTURAL PARA DICHA NITRILASA, MODIFICAR DICHO GEN ESTRUCTURAL POR SUSTITUCION, TRUNCAMIENTO O EXTENSION PARA HACER QUE PRODUZCA NITRILASA MODIFICADA, Y CONSTRUIR UN PLASMIDO CAPAZ DE REPLICACION EN ESCHERICHIA COLI O EN AGROBACTERIUM TUMEFACIENS Y QUE COMPRENDE UN CASETE DE EXPRESION CON UNA REGION DE INICIACION DE LA TRANSCRIPCION PROVENIENTE DEL GEN CODIFICADOR DE LA OPINA. EL INVENTO ES UTIL PARA DESARROLLAR PLANTAS RESISTENTES AL EFECTO CITOTOXICO DEL BROMOXINILO.
ES8802134A 1987-07-08 1988-07-07 Un metodo para producir una nitrilasa especifica capaz de hidrolizar un p-hidroxibenzonitrilo dihalogenado en 3,5. Expired ES2010763A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/071,146 US4810648A (en) 1986-01-08 1987-07-08 Haloarylnitrile degrading gene, its use, and cells containing the gene

Publications (1)

Publication Number Publication Date
ES2010763A6 true ES2010763A6 (es) 1989-12-01

Family

ID=22099529

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8802134A Expired ES2010763A6 (es) 1987-07-08 1988-07-07 Un metodo para producir una nitrilasa especifica capaz de hidrolizar un p-hidroxibenzonitrilo dihalogenado en 3,5.

Country Status (15)

Country Link
US (1) US4810648A (es)
EP (1) EP0373173A1 (es)
KR (1) KR890701735A (es)
CN (1) CN1031252A (es)
AU (1) AU2071988A (es)
BR (1) BR8807609A (es)
CA (1) CA1339683C (es)
DD (2) DD284048A5 (es)
DK (1) DK2890A (es)
ES (1) ES2010763A6 (es)
GR (1) GR880100421A (es)
IL (1) IL87001A (es)
PL (1) PL273589A1 (es)
WO (1) WO1989000193A1 (es)
ZA (1) ZA884894B (es)

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