EP4105319A4 - Verfahren zur unterdrückung der differenzierung pluripotenter stammzellen - Google Patents

Verfahren zur unterdrückung der differenzierung pluripotenter stammzellen

Info

Publication number
EP4105319A4
EP4105319A4 EP21754008.7A EP21754008A EP4105319A4 EP 4105319 A4 EP4105319 A4 EP 4105319A4 EP 21754008 A EP21754008 A EP 21754008A EP 4105319 A4 EP4105319 A4 EP 4105319A4
Authority
EP
European Patent Office
Prior art keywords
stem cells
pluripotent stem
suppressing differentiation
differentiation
suppressing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21754008.7A
Other languages
English (en)
French (fr)
Other versions
EP4105319A1 (de
Inventor
Kazuhiro Takeuchi
Masato Ibuki
Yohei Hayashi
Mami TAKASAKI
Sho KAMBAYASHI
Yoshikazu Kawai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaneka Corp
RIKEN Institute of Physical and Chemical Research
Original Assignee
Kaneka Corp
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp, RIKEN Institute of Physical and Chemical Research filed Critical Kaneka Corp
Publication of EP4105319A1 publication Critical patent/EP4105319A1/de
Publication of EP4105319A4 publication Critical patent/EP4105319A4/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP21754008.7A 2020-02-12 2021-02-12 Verfahren zur unterdrückung der differenzierung pluripotenter stammzellen Pending EP4105319A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020021843 2020-02-12
PCT/JP2021/005255 WO2021162090A1 (ja) 2020-02-12 2021-02-12 多能性幹細胞の分化抑制方法

Publications (2)

Publication Number Publication Date
EP4105319A1 EP4105319A1 (de) 2022-12-21
EP4105319A4 true EP4105319A4 (de) 2024-03-20

Family

ID=77292727

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21754008.7A Pending EP4105319A4 (de) 2020-02-12 2021-02-12 Verfahren zur unterdrückung der differenzierung pluripotenter stammzellen

Country Status (5)

Country Link
US (1) US20230094568A1 (de)
EP (1) EP4105319A4 (de)
JP (1) JPWO2021162090A1 (de)
CN (1) CN115135755A (de)
WO (1) WO2021162090A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3587560A4 (de) * 2017-01-27 2020-12-16 Kaneka Corporation Endodermale zellmasse und verfahren zur herstellung einer beliebigen von drei primärkeimschichtzellmassen aus pluripotenten zellen
WO2023120420A1 (ja) * 2021-12-20 2023-06-29 株式会社カネカ 多能性幹細胞ストックの大量製造方法
WO2023149495A1 (ja) * 2022-02-04 2023-08-10 国立研究開発法人理化学研究所 人工多能性幹細胞の製造方法
CN114591894B (zh) * 2022-02-28 2024-01-09 中国人民解放军总医院 一种皮肤多能前体干细胞的制备方法及其应用
WO2024101385A1 (ja) * 2022-11-09 2024-05-16 株式会社カネカ 多能性幹細胞の製造方法及び多能性幹細胞に対する分化誘導方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170275593A1 (en) * 2014-07-30 2017-09-28 Yeda Research And Development Co. Ltd. Media for culturing pluripotent stem cells
WO2019131940A1 (ja) * 2017-12-28 2019-07-04 株式会社カネカ 多能性幹細胞凝集抑制剤

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2746482T3 (es) * 2010-09-07 2020-03-06 Technion Res & Dev Foundation Nuevos métodos y medios de cultivo para cultivar células madre pluripotentes
US20140329317A1 (en) * 2011-11-25 2014-11-06 Kyoto University Method for culturing pluripotent stem cell
JPWO2019151386A1 (ja) * 2018-01-31 2021-01-07 富士フイルム株式会社 細胞の製造方法
JP7022340B2 (ja) 2018-08-01 2022-02-18 日本電気硝子株式会社 カメラのキャリブレーション方法、キャリブレーション装置及びキャリブレーション用ターゲット
WO2021020267A1 (ja) * 2019-07-26 2021-02-04 富士フイルム株式会社 ナイーブ型多能性幹細胞の増殖能亢進用培地および多能性幹細胞の製造方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170275593A1 (en) * 2014-07-30 2017-09-28 Yeda Research And Development Co. Ltd. Media for culturing pluripotent stem cells
WO2019131940A1 (ja) * 2017-12-28 2019-07-04 株式会社カネカ 多能性幹細胞凝集抑制剤
US20220267736A1 (en) * 2017-12-28 2022-08-25 Kaneka Corporation Pluripotent stem cell aggregation suppressor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GE GUO ET AL: "Epigenetic resetting of human pluripotency", DEVELOPMENT, vol. 144, no. 15, 31 August 2017 (2017-08-31), GB, pages 2748 - 2763, XP055456607, ISSN: 0950-1991, DOI: 10.1242/dev.146811 *
See also references of WO2021162090A1 *

Also Published As

Publication number Publication date
US20230094568A1 (en) 2023-03-30
WO2021162090A1 (ja) 2021-08-19
CN115135755A (zh) 2022-09-30
JPWO2021162090A1 (de) 2021-08-19
EP4105319A1 (de) 2022-12-21

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