EP4011886A1 - Tetrazyklische verbindung, herstellungsverfahren dafür und verwendung da - Google Patents
Tetrazyklische verbindung, herstellungsverfahren dafür und verwendung da Download PDFInfo
- Publication number
- EP4011886A1 EP4011886A1 EP20850862.2A EP20850862A EP4011886A1 EP 4011886 A1 EP4011886 A1 EP 4011886A1 EP 20850862 A EP20850862 A EP 20850862A EP 4011886 A1 EP4011886 A1 EP 4011886A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- compound
- alkyl
- pharmaceutically acceptable
- acceptable salt
- membered heterocycloalkyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D498/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains three hetero rings
- C07D498/16—Peri-condensed systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
- A61K31/551—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole having two nitrogen atoms, e.g. dilazep
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
- A61K31/553—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole having at least one nitrogen and one oxygen as ring hetero atoms, e.g. loxapine, staurosporine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/04—Ortho-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed systems contains four or more hetero rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/12—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains three hetero rings
- C07D487/16—Peri-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D498/22—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains four or more hetero rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
- C07D513/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains three hetero rings
- C07D513/16—Peri-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
- C07D513/22—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains four or more hetero rings
Definitions
- the present disclosure relates to a compound of formula (I), an optical isomer thereof and a pharmaceutically acceptable salt thereof, and use of the compound as a KRAS inhibitor.
- Cancer has been the first cause of death in China for 31 years.
- lung cancer is one of the most common tumors, and more than 80% of lung cancers are non-small cell lung cancer.
- the incidence of lung cancer is high, with a variety of mutations present.
- focusing on unmet medical needs and developing innovative drugs for treating cancer are very necessary for the long-term development of the company and are of great economic and social significance.
- RAS genes Mutations in RAS genes are found in about 30% of cancer patients. In the research of oncogenes, scientists found that RAS genes were key genes to cancers including lung cancer, colorectal cancer and pancreatic cancer over 20 years ago.
- RAS mutations are also the most common in the three types of cancer with the highest mortality (pancreatic, colorectal and lung cancers), and are found in 95%, 52% and 31% of patients with these three types of cancer, respectively.
- KRAS mutations predominate in pancreatic, colorectal and lung cancers
- NRAS mutations are common in melanoma and acute myeloid leukemia
- HRAS mutations are common in bladder and head and neck cancers.
- KRAS genes in Asian population are 10-15%.
- KRAS genes mutate in many cancers, and are one of the main oncogenes.
- KRAS mutant tumors are the most potentially targeted molecular subtype of non-small cell lung cancer (NSCLC), with a mutation rate of about 15% to 25% in NSCLC.
- NSCLC non-small cell lung cancer
- KRAS mutations mainly occur at codons 12 and 13.
- KRAS-G12C mutations, the most common codon mutations, are found in approximately 39% of KRAS mutant NSCLCs.
- KRAS gene testing must be done prior to the treatment of a lung cancer patient with EGFR-TKIs, and whether to use targeted drugs EGFR-TKIs as clinical treatment measures is determined based on the testing results. If KRAS genes have mutated, molecular targeted therapies with EGFR-TKIs are not recommended for the patient.
- KRAS small molecule drugs including 10 KRAS GTP enzyme inhibitors, 4 KRAS gene inhibitors, 2 KRAS GTP enzyme regulators and 2 KRAS gene regulators; currently, there are 1 of such drugs in clinical research.
- the first KRAS inhibitor Antroquinonol developed by Taiwan corporation has entered the U.S. FDA phase II clinical trials, and the inhibitor selumetinib targeting MEK in the KRAS downstream pathway developed by AstraZeneca is also in phase II clinical trials. KRAS mutations are the most important tumor driver genes.
- the mutation cases account for a certain proportion in patients with pancreatic cancer, lung cancer and colorectal cancer. At present, no specifically targeted drug acting on this target is available. Therefore, the project has important medical research value and clinical application value, and has greater medical value for people in China.
- a KRAS-G12C small molecule drug its molecular mechanism has been substantially elucidated, and the molecular structure and efficacy of the drug are verified under the existing test conditions, showing that it has high activity and druggability.
- the present disclosure provides a compound of formula (I), an optical isomer and a pharmaceutically acceptable salt thereof, wherein,
- R is independently selected from H, halogen, OH, NH 2 , CN, C 1-3 alkyl, C 1-3 alkoxy, C 1-3 alkylthio, C 1-3 alkylamino and 5-6 membered heterocycloalkyl, wherein the C 1-3 alkyl, C 1-3 alkoxy, C 1-3 alkylthio, C 1-3 alkylamino, or 5-6 membered heterocycloalkyl is optionally substituted with 1, 2, or 3 R', and the other variables are as defined in the present disclosure.
- R is independently selected from H, F, Cl, Br, I, OH, NH 2 , CN, Me, and wherein Me, is optionally substituted with 1, 2 or 3 R', and the other variables are as defined in the present disclosure.
- R is independently selected from H, F, Cl, Br, I, OH, NH 2 , CN, Me, and the other variables are as defined in the present disclosure.
- R 1 , R 2 and R 11 are each independently selected from H, halogen, OH, NH 2 , CN, C 1-3 alkyl, C 1-3 alkoxy, and C 1-3 alkylamino, wherein C 1-3 alkyl, C 1-3 alkoxy or C 1-3 alkylamino is optionally substituted with 1, 2, or 3 R, and the other variables are as defined in the present disclosure.
- R 1 , R 2 and R 11 are each independently selected from H, F, Cl, Br, I, OH, NH 2 , CN, Me, CF 3 , and and the other variables are as defined in the present disclosure.
- R 3 and R 4 are each independently selected from H, F, Cl, Br, I, OH, NH 2 , CN, Me, CF 3 , and and the other variables are as defined in the present disclosure.
- R 5 is selected from H, F, Cl, Br, I, OH, NH 2 , CN, Me, CF 3 , and the other variables are as defined in the present disclosure.
- R 6 is selected from H, F, Cl, Br, I, OH, NH 2 , CN, Me, CF 3 , N(CH 3 ) 2 , and the other variables are as defined in the present disclosure.
- ring A is selected from C 3-6 cycloalkyl, 3-6 membered heterocycloalkyl, phenyl, naphthyl, thienyl, pyrazolyl, thiazolyl, imidazolyl, pyridyl, pyrimidinyl, indazolyl and indolyl, and the other variables are as defined in the present disclosure.
- the moiety is selected from and and the other variables are as defined in the present disclosure.
- ring B is selected from phenyl, naphthyl, thienyl, pyridyl, pyrimidinyl, indazolyl, indolyl, 1 H -benzo[ d ][1,2,3]triazolyl, 1,3-dihydro-2 H -benzo[ d ]imidazol-2-onyl, benzo[d]oxazol-2(3 H )-onyl, 1 H -pyrazolo[3,4-b]pyridyl, isoquinolin-1(2 H )-onyl and 1 H -benzo[d]imidazolyl, and the other variables are as defined in the present disclosure.
- the moiety is selected from and the other variables are as defined in the present disclosure.
- the moiety is selected from and the other variables are as defined in the present disclosure.
- the moiety is selected from and the other variables are as defined in the present disclosure.
- R 7 is selected from H, F, Cl, Br, I, CN, Me, CF 3 , and the other variables are as defined in the present disclosure.
- R 8 and R 9 are each independently selected from H, F, Cl, Br, I, CN, Me, CF 3 , the other variables are as defined in the present disclosure.
- the moietys is selected from and the other variables are as defined in the present disclosure.
- the compound, the optical isomer thereof and the pharmaceutically acceptable salt thereof are selected from wherein, R 1 , R 2 , L 1 , L 2 , T, T 2 , R 5 , R 6 , ring A, ring B, R 7 , R 8 and R 9 are as defined above.
- the compound, the optical isomer thereof and the pharmaceutically acceptable salt thereof are selected from wherein, R 1 , R 2 , L 1 , T, T 2 , R 5 , R 6 , ring A, ring B, R 7 , R 8 and R 9 are as defined above.
- the compound, the optical isomer thereof and the pharmaceutically acceptable salt thereof are selected from wherein, R 1 , R 2 , L 1 , T, T 2 , R 5 , R 6 , ring A, ring B, R 7 , R 8 and R 9 are as defined above.
- the present disclosure also provides a compound of the formula below, an optical isomer thereof and a pharmaceutically acceptable salt thereof, which are selected from
- the present disclosure also provides a pharmaceutical composition
- a pharmaceutical composition comprising a therapeutically effective amount of the compound or the pharmaceutically acceptable salt thereof described above, and one or more pharmaceutically acceptable carriers, diluents or excipients.
- the present disclosure also provides use of the compound or the pharmaceutically acceptable salt thereof described above or the pharmaceutical composition described above in preparing a medicament for preventing and/or treating a KRAS-G12C-related disease.
- the KRAS-G12C-related disease is selected from non-small cell lung cancer, colon cancer and pancreatic cancer.
- pharmaceutically acceptable is used herein for those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problems or complications, and commensurate with a reasonable benefit/risk ratio.
- pharmaceutically acceptable salt refers to a salt of the compound of the present disclosure, which is prepared from the compound having particular substituents of the present disclosure and a relatively nontoxic acid or base.
- a base addition salt can be obtained by contacting the neutral form of such a compound with a sufficient amount of a base in a pure solution or a suitable inert solvent.
- Pharmaceutically acceptable base addition salts include sodium, potassium, calcium, ammonium, organic amine, or magnesium salts, or similar salts.
- an acid addition salt can be obtained by contacting the neutral form of such a compound with a sufficient amount of an acid in a pure solution or a suitable inert solvent.
- pharmaceutically acceptable acid addition salts include salts derived from inorganic acids, such as hydrochloric acid, hydrobromic acid, nitric acid, carbonic acid, bicarbonate radical, phosphoric acid, monohydrogen phosphate, dihydrogen phosphate, sulfuric acid, hydrogen sulfate, hydroiodic acid and phosphorous acid; and salts derived from organic acids, such as acetic acid, propionic acid, isobutyric acid, maleic acid, malonic acid, benzoic acid, succinic acid, suberic acid, fumaric acid, lactic acid, mandelic acid, phthalic acid, benzenesulfonic acid, p -toluenesulfonic acid, citric acid, tartaric acid and methanesulfonic
- salts of amino acids e.g., arginine
- salts of organic acids such as glucuronic acid.
- Certain specific compounds of the present disclosure contain both basic and acidic functional groups that allow the compounds to be converted into either base or acid addition salts.
- the pharmaceutically acceptable salts of the present disclosure can be synthesized from a parent compound having an acidic or basic group by conventional chemical methods.
- such salts are prepared by the following method: performing a reaction of the free acid or base form of the compound and a stoichiometric amount of an appropriate base or acid in water or an organic solvent or a mixture thereof.
- the compound of the present disclosure may have a specific geometric or stereoisomeric form. All such compounds are contemplated in the present disclosure, including cis and trans isomers, (-)- and (+)-enantiomers, ( R )- and ( S )-enan tiomers, diastereoisomers, ( D )-isomers, ( )-isomers, and racemic mixtures and other mixtures thereof, such as an enantiomer or diastereomer enriched mixture, all of which are encompassed within the scope of the present disclosure
- Substituents such as alkyl may have an additional asymmetric carbon atom All these isomers and mixtures thereof are encompassed within the scope of the present disclosure.
- enantiomer or “optical isomer” refers to stereoisomers that are mirro images of each other.
- cis-trans isomer or "geometric isomer” results from the inability of a single bond of a ring carbon atom or a double bond to rotate freely.
- diastereomer refers to stereoisomers whose molecules have two or more chiral centers and are not mirror images of each other.
- tautomer or "tautomeric form” means that different functional isomers are in dynamic equilibrium at room temperature and can be rapidly converted into each other If tautomers are possible (e.g., in solution), the chemical equilibrium of the tautomers can be achieved
- a proton tautomer also known as a prototropic tautomer, includes interconversion by proton migration, such as keto-enol isomerism and imine-enamine isomerism
- a valence tautomer includes interconversion by recombination of some bonding electrons
- a specific example of the keto-enol tautomerism is the interconversion between the tautomers pentane-2,4-dione and 4-nydroxypent-3-en-2-one.
- the compound of the present disclosure may contain an unnatural proportion of atomic isotope at one or more of the atoms that constitute the compound.
- the compound may be labeled with a radioisotope, such as tritium ( 3 H), iodine-125 ( 125 I), or C-14 ( 14 C).
- hydrogen can be substituted by deuterium to form a deuterated drug, and the bond formed by deuterium and carbon is firmer than that formed by common hydrogen and carbon Compared with an un- leuterated drug, the deuterated drug has the advantages of reduced toxic side effect, increased stability, enhanced efficacy, and prolonged biological half-life and the like All isotopic variations of the compounds of the present disclosure, whether radioactive o not, are encompassed within the scope of the present disclosure "Optional” or “optionally” means that the subsequently described event or circumstance may, but does not necessarily, occur, and the description includes instances where the event or circumstance occurs and instances where it does not.
- substituted means that one or more hydrogen atoms on a specific atom are substituted by substituents which may include deuterium and hydrogen variants, as long as the valence of the specific atom is normal and the substituted compound is stable.
- substituents which may include deuterium and hydrogen variants, as long as the valence of the specific atom is normal and the substituted compound is stable.
- substitution with oxygen does not occur on aromatic groups.
- optionally substituted means that an atom can be substituted with a substituent or not. Unless otherwise specified, the type and number of the substituent may be arbitrary as long as being chemically achievable.
- variable e.g., R
- the variable is independently defined in each case.
- the group can be optionally substituted with up to two R, and the definition of R in each case is independent.
- a combination of a substituent and/or a variant thereof is permissible only if the combination can result in a stable compound.
- the substituent can be linked via any atom of the group.
- pyridyl as a substituent can be linked to the group to be substituted via any carbon atom on the pyridine ring.
- the linking direction of the linking group listed is not specified, the linking direction is arbitrary.
- the linking group L in can either link the benzene ring and the cyclohexane in a direction same as left-to-right reading order to form or link the benzene ring and the cyclohexane in a direction opposite to left-to-right reading order to form
- a substituent and/or a variant thereof is permissible only if the combination can result in a stable compound.
- the number of atoms on a ring is generally defined as the member number of the ring.
- “5-7 membered ring” refers to a “ring” on which 5 to 7 atoms are arranged in a circle.
- C 1-6 alkyl refers to a linear or branched saturated hydrocarbon group consisting of 1 to 6 carbon atoms.
- the C 1-6 alkyl includes C 1-5 , C 1-4 , C 1-3 , C 1-2 , C 2-6 , C 2-4 , C 6 , and C 5 alkyl, etc., and may be monovalent (e.g., methyl), divalent (e.g., methylene), or polyvalent (e.g., methenyl).
- C 1-6 alkyl examples include, but are not limited to, methyl (Me), ethyl (Et), propyl (including n -propyl and isopropyl), butyl (including n -butyl, isobutyl, s-butyl, and t -butyl), pentyl (including n -pentyl, isopentyl, and neopentyl), and hexyl and the like.
- C 1-5 alkyl refers to a linear or branched saturated hydrocarbon group consisting of 1 to 5 carbon atoms.
- the C 1-5 alkyl includes C 1-4 , C 1-3 , C 1-2 , C 2-5 , C 2-4 and C 5 alkyl and the like, and may be monovalent (e.g., methyl), divalent (e.g., methylene) or polyvalent (e.g., methenyl).
- C 1-5 alkyl examples include, but are not limited to, methyl (Me), ethyl (Et), propyl (including n -propyl and isopropyl), butyl (including n -butyl, isobutyl, s-butyl, and butyl), and pentyl (including n -pentyl, isopentyl, and neopentyl) and the like.
- C 1-4 alkyl refers to a linear or branched saturated hydrocarbon group consisting of 1 to 4 carbon atoms.
- the C 1-4 alkyl includes, but is not limited to, C 1-2 , C 1-3 , and C 2-3 alkyl and the like, and may be monovalent (e.g., methyl), divalent (e.g., methylene) or polyvalent (e.g., methenyl).
- C 1-4 alkyl examples include, but are not limited to, methyl (Me), ethyl (Et), propyl (including n -propyl and isopropyl), and butyl (including n -butyl, isobutyl, s-butyl and t -butyl) and the like.
- C 1-3 alkyl refers to a linear or branched saturated hydrocarbon group consisting of 1 to 3 carbon atoms.
- the C 1-3 alkyl includes, but is not limited to, C 1-2 and C 2-3 alkyl, etc., and may be monovalent (e.g., methyl), divalent (e.g., methylene), or polyvalent (e.g., methenyl).
- Examples of C 1-3 alkyl include, but are not limited to, methyl (Me), ethyl (Et), and propyl (including n -propyl and isopropyl).
- C 2-8 alkenyl is used to denote a linear or branched hydrocarbon group containing 2 to 8 carbon atoms and at least one carbon-carbon double bond, which may be located anywhere in the group.
- the C 2-8 alkenyl includes C 2-6 , C 2-4 , C 2-3 , C 4 , C 3 , and C 2 alkenyl etc., and may be monovalent, divalent or polyvalent.
- Examples of C 2-8 alkenyl include, but are not limited to, ethenyl, propenyl, butenyl, pentenyl, hexenyl, butadienyl, 1,3-pentadienyl, and 1,3-hexadienyl and the like.
- C 2-4 alkenyl is used to denote a linear or branched hydrocarbon group containing 2 to 4 carbon atoms and at least one carbon-carbon double bond, which may be located anywhere in the group.
- the C 2-4 alkenyl includes C 2-3 , C 4 , C 3 and C 2 alkenyl and the like, and may be monovalent, divalent or polyvalent.
- Examples of C 2-4 alkenyl include, but are not limited to, ethenyl, propenyl, butenyl, and butadienyl and the like.
- C 2-3 alkenyl is used to denote a linear or branched hydrocarbon group containing 2 to 3 carbon atoms and at least one carbon-carbon double bond, which may be located anywhere in the group.
- the C 2-3 alkenyl includes C 3 and C 2 alkenyl, and may be monovalent, divalent or polyvalent. Examples of C 2-3 alkenyl include, but are not limited to, ethenyl, and propenyl and the like.
- C 2-4 alkynyl is used to denote a linear or branched hydrocarbon group containing 2 to 4 carbon atoms and at least one carbon-carbon triple bond, which may be located anywhere in the group.
- the C 2-4 alkynyl includes C 2-3 , C 4 , C 3 and C 2 alkynyl and the like. It may be monovalent, divalent or polyvalent. Examples of C 2-4 alkynyl include, but are not limited to, ethynyl, propynyl, and butynyl and the like.
- C 2-3 alkynyl is used to denote a linear or branched hydrocarbon group containing 2 to 3 carbon atoms and at least one carbon-carbon triple bond, which may be located anywhere in the group. It may be monovalent, divalent or polyvalent.
- the C 2-3 alkynyl includes C 3 and C 2 alkynyl. Examples of C 2-3 alkynyl include, but are not limited to, ethynyl, and propynyl and the like.
- heteroalkyl by itself or in combination with another term, refers to a stable linear or branched alkyl radical or a combination thereof consisting of a specified number of carbon atoms and at least one heteroatom or heteroatom group.
- the heteroatom is selected from B, O, N, and S, wherein nitrogen and sulfur atoms are optionally oxidized and the nitrogen heteroatom is optionally quaternized.
- the heteroalkyl is C 1-6 heteroalkyl. In other embodiments, the heteroalkyl is C 1-3 heteroalkyl.
- heteroatom or heteroatom group can be located at any interior position of heteroalkyl, including the position where the alkyl is linked to the rest part of the molecule.
- alkoxy alkylamino
- alkylthio or thioalkxoy are commonly used expressions and refer to those alkyl groups linked to the rest part of the molecule via an oxygen atom, an amino, or a sulfur atom, respectively.
- At most two heteroatoms can be
- C 1-6 alkoxy refers to those alkyl groups that each contains 1 to 6 carbon atoms and is linked to the rest part of the molecule through an oxygen atom.
- the C 1-6 alkoxy includes C 1-4 , C 1-3 , C 1-2 , C 2-6 , C 2-4 , C 6 , C 5 , C 4 and C 3 alkoxy and the like.
- C 1-6 alkoxy examples include, but are not limited to, methoxy, ethoxy, propoxy (including n -propoxy and isopropoxy), butoxy (including n -butoxy, isobutoxy, s-butoxy and t -butoxy), pentyloxy (including n -pentyloxy, isopentyloxy and neopentyloxy), and hexyloxy and the like.
- C 1-3 alkoxy refers to those alkyl groups that each contains 1 to 3 carbon atoms and is linked to the rest part of the molecule through an oxygen atom.
- the C 1-3 alkoxy includes C 1-2 , C 2-3 , C 3 and C 2 alkoxy and the like.
- Examples of C 1-3 alkoxy include, but are not limited to, methoxy, ethoxy, and propoxy (including n-propoxy and isopropoxy) and the like.
- C 1-6 alkylamino refers to those alkyl groups that each contains 1 to 6 carbon atoms and is linked to the rest part of the molecule through an amino group.
- the C 1-6 alkylamino includes C 1-4 , C 1-3 , C 1-2 , C 2-6 , C 2-4 , C 6 , C 5 , C 4 , C 3 and C 2 alkylamino and the like.
- C 1-6 alkylamino examples include, but are not limited to, - NHCH 3 , -N(CH 3 ) 2 , -NHCH 2 CH 3 , -N(CH 3 )CH 2 CH 3 , -N(CH 2 CH 3 )(CH 2 CH3), - NHCH 2 CH 2 CH 3 , -NHCH 2 (CH 3 ) 2 , and -NHCH 2 CH 2 CH 2 CH 3 and the like.
- C 1-3 alkylamino refers to those alkyl groups that each contains 1 to 3 carbon atoms and is linked to the rest part of the molecule through an amino group.
- the C 1-3 alkylamino includes C 1-2 , C 3 and C 2 alkylamino and the like.
- Examples of C 1-3 alkylamino include, but are not limited to, -NHCH 3 , -N(CH 3 ) 2 , -NHCH 2 CH 3 , -N(CH 3 )CH 2 CH 3 , and -NHCH 2 CH 2 CH 3 , -NHCH 2 (CH 3 ) 2 and the like.
- C 1-6 alkylthio refers to those alkyl groups that each contains 1 to 6 carbon atoms and is linked to the rest part of the molecule through a sulfur atom.
- the C 1-6 alkylthio includes C 1-4 , C 1-3 , C 1-2 , C 2-6 , C 2-4 , C 6 , C 5 , C 4 , C 3 and C 2 alkylthio and the like.
- Examples of C 1-6 alkylthio include, but are not limited to, -SCH 3 , -SCH 2 CH 3 , - SCH 2 CH 2 CH 3 , and -SCH 2 (CH 3 ) 2 and the like.
- C 1-3 alkylthio refers to those alkyl groups that each contains 1 to 3 carbon atoms and is linked to the rest part of the molecule through a sulfur atom.
- the C 1-3 alkylthio includes C 1-3 , C 1-2 and C 3 alkylthio and the like. Examples of C 1-3 alkylthio include, but are not limited to, -SCH 3 , -SCH 2 CH 3 , -SCH 2 CH 2 CH 3 , and -SCH 2 (CH 3 ) 2 and the like.
- C 3-6 cycloalkyl refers to a saturated cyclic hydrocarbon group consisting of 3 to 6 carbon atoms, including monocyclic and bicyclic ring systems.
- the C 3-6 cycloalkyl includes C 3-5 cycloalkyl, C 4-5 cycloalkyl, C 5-6 cycloalkyl and the like, and may be monovalent, divalent or polyvalent.
- Examples of C 3-6 cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like.
- 3-6 membered heterocycloalkyl refers to a saturated cyclic group consisting of 3 to 6 ring atoms, of which 1, 2, 3, or 4 ring atoms are heteroatoms independently selected from the group consisting of O, S and N, with the remaining being carbon atoms.
- the nitrogen atom is optionally quaternized, and the nitrogen and sulfur heteroatoms can be optionally oxidized (i.e., NO and S(O) p , where p is 1 or 2).
- a heteroatom may occupy the position where the heterocycloalkyl is linked to the rest of the molecule.
- the 3-6 membered heterocycloalkyl includes 4-6 membered, 5-6 membered, 4 membered, 5 membered, 6 membered heterocycloalkyl, and the like.
- Examples of 3-6 membered heterocycloalkyl include, but are not limited to, azetidinyl, oxetanyl, thietanyl, pyrrolidinyl, pyrazolidinyl, imidazolidinyl, tetrahydrothienyl (including tetrahydrothien-2-yl, tetrahydrothien-3-yl, etc.), tetrahydrofuranyl (including tetrahydrofuran-2-yl, etc.), tetrahydropyranyl, piperidinyl (including 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, etc.), piperazinyl (including 1-piperazinyl, 2-piperazinyl, etc.), morpholinyl (including 3-morpholinyl, 4-morpholinyl, etc.), dioxanyl, dithianyl, isoxazolidinyl, isothiazolidinyl, 1,
- C 6-10 aromatic ring and “C 6-10 aryl” of the present disclosure are used interchangeably.
- the term “C 6-10 aromatic ring” or “C 6-10 aryl” refers to a cyclic hydrocarbon group consisting of 6 to 10 carbon atoms and having a conjugated ⁇ -electron system. The group may be a monocyclic, fused bicyclic or fused tricyclic system, where the rings are aromatic. It may be monovalent, divalent or polyvalent, and the C 6-10 aryl includes C 6-9 , C 9 , C 10 and C 6 aryl groups, etc. Examples of C 6-10 aryl include, but are not limited to, phenyl, naphthyl (including 1-naphthyl, 2-naphthyl, etc.).
- 5-10 membered heteroaromatic ring and “5-10 membered heteroaryl” of the present disclosure are used interchangeably.
- the term “ 5-10 membered heteroaryl” refers to a cyclic group consisting of 5 to 10 ring atoms and having a conjugated pi-electron system, in which 1, 2, 3, or 4 ring atoms are heteroatoms independently selected from the group consisting of O, S and N, while the others are carbon atoms. It can be a monocyclic, fused bicyclic or fused tricyclic system, wherein the rings are aromatic.
- the nitrogen atom is optionally quaternized, and the nitrogen and sulfur heteroatoms are optionally oxidized (i.e., NO and S(O) p , where p is 1 or 2).
- the 5-10 membered heteroaryl can be linked to the rest of the molecule via a heteroatom or a carbon atom.
- the 5-10 membered heteroaryl includes 5-8 membered, 5-7 membered, 5-6 membered, 5 membered and 6 membered heteroaryl groups, etc.
- Examples of the 5-10 membered heteroaryl include, but are not limited to, pyrrolyl (including N -pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, etc.), pyrazolyl (including 2-pyrazolyl, 3-pyrazolyl, etc.), imidazolyl (including N -imidazolyl, 2-imidazolyl, 4-imidazolyl, 5-imidazolyl, etc.), oxazolyl (including 2-oxazolyl, 4-oxazolyl, 5-oxazolyl, etc.), triazolyl (including 1 H -1,2,3-triazolyl, 2 H -1,2,3-triazolyl, 1 H -1,2,4-triazolyl, 4 H -1,2,4-triazolyl, etc.), tetrazolyl, isoxazolyl (including 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl, etc.), thiazolyl (including
- 5-6 membered heteroaromatic ring and “5-6 membered heteroaryl” of the present disclosure are used interchangeably.
- the term “5-6 membered heteroaryl” refers to a monocyclic group consisting of 5 to 6 ring atoms and having a conjugated ⁇ -electron system, in which 1, 2, 3 or 4 ring atoms are heteroatoms independently selected from the group consisting of O, S, and N, while the others are carbon atoms.
- the nitrogen atom is optionally quaternized, and the nitrogen and sulfur heteroatoms are optionally oxidized (i.e., NO and S(O) p , where p is 1 or 2).
- the 5-6 membered heteroaryl can be linked to the rest of the molecule via a heteroatom or a carbon atom.
- the 5-6 membered heteroaryl includes 5 membered and 6 membered heteroaryl.
- Examples of the 5-6 membered heteroaryl include, but are not limited to, pyrrolyl (including N -pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, etc.), pyrazolyl (including 2-pyrazolyl, 3-pyrazolyl, etc.), imidazolyl (including N -imidazolyl, 2-imidazolyl, 4-imidazolyl, 5-imidazolyl, etc.), oxazolyl (including 2-oxazolyl, 4-oxazolyl, 5-oxazolyl, etc.), triazolyl (including 1 H -1,2,3-triazolyl, 2 H -1,2,3-triazolyl, 1 H -1,2,4-triazolyl, 4 H -1,2,4-
- benzo 5-6 membered heterocycloalkyl refers to a double fused ring structure formed by the union of phenyl, heterocycle and 5-6 membered heterocycloalkyl, and the substituent can be linked to other structures through a benzene ring or 5-6 membered heterocycloalkyl ring.
- benzo 5-6 membered heterocycloalkyl include, but are not limited to and and the like.
- 5-6 membered heteroary-fused 5-6 membered heterocycloalkyl refers to a double fused ring structure formed by the union of 5-6 membered heteroaryl and heterocycle and 5-6 membered heterocycloalkyl, and the substituent can be linked to other structures through 5-6 membered heteroaryl or 5-6 membered heterocycloalkyl ring.
- benzo 5-6 membered heterocycloalkyl include, but are not limited to, and the like.
- C n-n+m or C n -C n+m includes any one of the specific cases of n to n+m carbon atoms.
- C 1-12 includes C 1 , C 2 , C 3 , C 4 , C 5 , C 6 , C 7 , C 8 , C 9 , C 10 , C 11 and C 12 .
- any range within n to n+m may be included.
- C 1-12 includes C 1-3 , C 1-6 , C 1-9 , C 3-6 , C 3-9 , C 3-12 , C 6-9 , C 6-12 and C 9-12 .
- n-n+m membered represents that the number of atoms on the ring is n to n+m.
- 3-12 membered ring includes 3 membered ring, 4 membered ring, 5 membered ring, 6 membered ring, 7 membered ring, 8 membered ring, 9 membered ring, 10 membered ring, 11 membered ring and 12 membered ring.
- n-n+m membered also represents any range within n to n+m.
- 3-12 membered ring includes 3-6 membered ring, 3-9 membered ring, 5-6 membered ring, 5-7 membered ring, 6-7 membered ring, 6-8 membered ring, and 6-10 membered ring.
- the compounds of the present disclosure can be prepared by a variety of synthetic methods well known to those skilled in the art, including the specific embodiments listed below, embodiments formed by combinations thereof with other chemical synthetic methods, and equivalents thereof known to those skilled in the art. Preferred embodiments include, but are not limited to, the examples of the present disclosure.
- CDCl 3 represents deuterated chloroform
- CD 3 OD represents deuterated methanol
- DMSO-d 6 represents deuterated dimethyl sulfoxide
- TBS represents tert -butyldimethylsilyl.
- the system was extracted with ethyl acetate (3 ⁇ 200 mL), and the organic phases were pooled, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to obtain a crude product.
- Diastereomer compound 1 (16 mg, 27.89 ⁇ mol) was purified by SFC (separation conditions: chromatographic column: DAICEL CHIRALCEL OJ-H (250 mm ⁇ 30 mm, 5 ⁇ m); mobile phase: [Neu-ethanol]; ethanol%: 30%-30%). After concentration, compound 1A (peak 1) and compound 1B (peak 2) were obtained.
- chromatographic column Waters Xbridge C18 3.5 ⁇ m, 150 ⁇ 4.6 mm; column temperature: 40 °C; mobile phase: water (0.05% aqueous ammonia)-acetonitrile; acetonitrile: 0%-95% 10 min, 95% 5 min; flow rate: 1.0 mL/min.
- chromatographic column Waters Xbridge C18 3.5 ⁇ m, 150 ⁇ 4.6 mm; column temperature: 40 °C; mobile phase: water (0.05% aqueous ammonia)-acetonitrile; acetonitrile: 0%-95% 10 min, 95% 5 min; flow rate: 1.0 mL/min.
- Diastereomer compound 2 was purified by SFC (separation conditions: chromatographic column: DAICEL CHIRALPAK AD-H (250 mm ⁇ 30 mm, 5 ⁇ m);mobile phase: [Neu-isopropanol (0.1% aqueous ammonia)]; isopropanol%: 35%). After concentration, compound 2A (peak 1) and compound 2B (peak 2) were obtained.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column ChiralpakAD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% in 2 min, 40% 1.2 min, 5% 0.8 min; flow rate: 4 mL/min.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column ChiralpakAD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% in 2 min, 40% 1.2 min, 5% 0.8 min; flow rate: 4 mL/min.
- Diisopropylethylamine (861.66 mg, 6.67 mmol, 1.16 mL) was dissolved in anhydrous tetrahydrofuran (40 mL). The resulting solution was cooled to -78 °C, and n -butyllithium (2.5 M, 26.67 mL) was added dropwise thereto. After the completion of the addition, the system was warmed to -30 °C and stirred for 10 min. The system was cooled to -78 °C, and a solution of compound 3-1 (7 g, 33.33 mmol) in tetrahydrofuran (40 mL) was added thereto. The system was stirred at -78 °C for 4 h.
- the filtrate was concentrated to obtain a crude product.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 10%-80%, 9.5 min) to obtain compound 3 in the form of a white solid.
- the system was adjusted to neutral pH with 1 N HCl and extracted with ethyl acetate (10 mL ⁇ 2).
- the organic phase was dried over anhydrous sodium sulfate and filtered.
- the filtrate was concentrated to obtain a crude product.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 10%-80% 9.5 min; flow rate: 30 mL/min) to obtain compound 4.
- Diastereomer compound 4 was purified by SFC (separation conditions: chromatographic column: DAICEL CHIRALPAK AD (250 mm ⁇ 30 mm, 10 ⁇ m); mobile phase: [Neu-isopropanol (0.1% aqueous ammonia)]; isopropanol%: 30%-30%; flow rate: 70 mL/min) After concentration, compound 4A (peak 1) and compound 4B (peak 2) were obtained.
- chromatographic column Ultimate C18 3.0 ⁇ 50 mm, 3 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 1.2 mL/min.
- chromatographic column Chiralpak AD-3 50 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% 2 min, 40% 1.2 min, 5% 0.8 min; flow rate: 4 mL/min.
- chromatographic column Ultimate C18 3.0 ⁇ 50 mm, 3 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 1.2 mL/min.
- chromatographic column ChiralpakAD-3 50 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% 2 min, 40% 1.2 min, 5% 0.8 min; flow rate: 4 mL/min.
- the organic phase was washed with saturated brine (30 mL), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to obtain a crude product.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 38%-68% 9.5 min; flow rate: 30 mL/min) to obtain compound 5.
- Diastereomer compound 4 was purified by SFC (separation conditions: chromatographic column: Phenomenex Lux Cellulose-4 250 ⁇ 30 mm ⁇ 5 ⁇ m; mobile phase: [Neu-ethanol (0.1% aqueous ammonia)]; ethanol%: 40%-40%, flow rate: 60 mL/min). After concentration, compound 5A (peak 1) and compound 5B (peak 2) were obtained.
- chromatographic column Ultimate C18 3.0 ⁇ 50 mm, 3 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 1.2 mL/min.
- chromatographic column Cellulose-4 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-ethanol (0.05% DEA)]; ethanol%: 40%; flow rate: 28 mL/min.
- chromatographic column Cellulose-4 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-ethanol (0.05% DEA)]; ethanol%: 40%; flow rate: 28 mL/min.
- the crude product was purified by p eparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 21%-51% 9.5 min; flow rate: 60 mL/min) to obtain compound 6.
- Diastereomer compound 6 was purified by SFC (separation conditions: chromatographic column: REGIS (s,s) WHELK- O1 (250 mm ⁇ 30 mm, 5 ⁇ m);mobile phase: [Neu-isopropanol (0.1% aqueous ammonia)]; isopropanol%: 50%-50%; flow rate: 80 mL/min). After concentrat on, compound 6A (peak 1) and compound 6B (peak 2) were obtained.
- chromatographic column Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 21%-51% 9.5 min; flow rate: 60 mL/min) to obtain compound 7.
- Diastereomer compound 7 was purified by SFC (separation conditions: chromatographic column: DAICEL CHIRALCEL OJ-H (250 mm ⁇ 30 mm, 5 ⁇ m); mobile phase: [Neu- sopropanol (0.1% aqueous ammonia)]; isopropanol%: 40%-40%). After concentration, compound 7A (peak 1) and compound 7B (peak B) were obtained.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.06875 % trifluoroacetic acid)-acetonitrile (0.0625 % trifluoroacetic acid); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Chiralcel OJ-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)], isopropanol%: 5%-40% 5 min, 40% 2.5 min, 5% 2.5 min; flow rate: 2.5 mL/min.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.06875 % trifluoroacetic acid)-acetonitrile (0.0625 % trifluoroacetic acid); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Chiralcel OJ-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)], isopropanol%: 5%-40% 5 min, 40% 2.5 min, 5% 2.5 min; flow rate: 2.5 mL/min.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Welch Xtimate C18 21.2 ⁇ 250 mm, 10 ⁇ m; column temperature: 25 °C, mobile phase: water (10 mM ammonium bicarbonate solution)-acetonitrile; mobile phase acetonitrile proportion 25%-45% in 12 min; flow rate: 30 mL/min) to obtain compound 8.
- Diastereomer compound 8 was purified by SFC (separation conditions: chromatographic column: ChiralPak AD, 300 ⁇ 50 mm I.D., 10 ⁇ m; column temperature: 38 °C; mobile phase [Neu-isopropanol (0.1% aqueous ammonia)]; isopropanol%: 35%; flow rate: 80 mL/min). After concentration, compound 8A (peak 1) and compound 8B (peak 2) were obtained.
- chromatographic column Chiralpak AD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% in 5 min, 40% 2.5 min; flow rate: 2.5 mL/min.
- chromatographic column Chiralpak AD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% in 5 min, 40% 2.5 min; flow rate: 2.5 mL/min.
- the system was adjusted to neutral pH with 1 N HCl and extracted with ethyl acetate (10 mL ⁇ 2).
- the organic phase was dried over anhydrous sodium sulfate and filtered.
- the filtrate was concentrated to obtain a crude product.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 36%-66% 9.5 min; flow rate: 30 mL/min) to obtain compound 9.
- the filtrate was concentrated to obtain a crude product.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 24%-54% 9.5 min) to obtain compound 10.
- Diastereomer compound 10 was purified by SFC (separation conditions: chromatographic column: DAICEL CHIRALPAK AD (250 mm ⁇ 30 mm, 10 ⁇ m); mobile phase: [Neu-isopropanol (0.1% aqueous ammonia)]; isopropanol%: 35%-35%; flow rate: 80 mL/min) After concentration, compound 10A (peak 1) and compound 10B (peak 2) were obtained.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroace ic acid solution); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Chiralpak AD-3 50 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% in 2 min, 40% 1.2 min, 5% 0.8 min; flow rate: 4 mL/min.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Chiralpak AD-3 50 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-isopropanol (0.05% DEA)]; isopropanol%: 5%-40% in 2 min, 40% 1.2 min, 5% 0.8 min; flow rate: 4 mL/min.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 42%-72%, 9.5 min) to obtain compound 11.
- Diastereomer compound 11 was purified by SFC (separation conditions: chromatographic column: DAICEL CHIRALCEL OD (250 mm ⁇ 30 mm, 10 ⁇ m); mobile phase: [Neu-ethanol (0.1% aqueous ammonia)]; ethanol%: 45%-45%) After concentration, compound 11A, compound 11B, compound 11C and compound 11D were obtained.
- chromatographic column Xbridge Shield RP-18, 5 ⁇ m, 2.1 ⁇ 50 mm; column temperature: 50 °C; mobile phase: water (0.02% aqueous ammonia)-acetonitrile; acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 0.8 mL/min.
- chromatographic column Chiralcel OD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-;thanol (0.05% DEA)]; ethanol%: 40%-40%; flow rate: 2.5 mL/min.
- chromatographic column Xbridge Shield RP-18, 5 ⁇ m, 2.1 ⁇ 50 mm; column temperature: 50 °C; mobile phase: water (0.02% aqueous ammonia)-acetonitrile; acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 0.8 mL/min.
- chromatographic column Chiralcel OD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-ethanol (0.05% DEA)]; ethanol%: 40%-40%; flow rate: 2.5 mL/min.
- chromatographic column Xbridge Shield RP-18, 5 ⁇ m, 2.1 ⁇ 50 mm; column temperature: 50 °C; mobile phase: water (0.02% aqueous ammonia)-acetonitrile; acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 0.8 mL/min.
- chromatographic column Chiralcel OD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-ethanol (0.05% DEA)]; ethanol%: 40%-40%; flow rate: 2.5 mL/min.
- chromatographic column Xbridge Shield RP-18, 5 ⁇ m, 2.1 ⁇ 50 mm; column temperature: 50 °C; mobile phase: water (0.02% aqueous ammonia)-acetonitrile; acetonitrile: 10%-80% 6 min, 80% 2 min; flow rate: 0.8 mL/min.
- chromatographic column Chiralcel OD-3 150 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-ethanol (0.05% DEA)]; ethanol%: 40%-40%; flow rate: 2.5 mL/min.
- compound 4-1 100 mg, 183.82 ⁇ mol
- compound 12-1 80 mg, 292.46 ⁇ mol
- 2-dicyclohexylphosphonium-2,4,6-triisopropylbiphenyl 20 mg, 41.95 ⁇ mol
- potassium carbonate 100 mg, 723.54 ⁇ mol
- Diastereomer compound 12 was purified by SFC (separation conditions: chromatographic column: Phenomenex-Cellulose-2 (250 mm ⁇ 50 mm, 10 ⁇ m); mobile phase: [Neu-methanol (0.1% aqueous ammonia)]; methanol%: 50%-50%) After concentration, compound 12A (peak 1) and compou d 12B (peak 2) were obtained.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.06875 % trifluoroacetic acid)-acetonitrile (0.0625 % trifluoro acetic acid); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Cellulose 2 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-methanol (0.05% DEA)]; methanol%: 40%-40%; flow rate: 2.8 mL/min.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.06875 % trifluoroacetic acid)-acetonitrile (0.0625 % trifluoroacetic acid); acetonitrile: 10%-80% 10 min, 80% 5 min; flow rate: 1.5 mL/min.
- chromatographic column Cellulose 2 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: [Neu-methanol (0.05% DEA)]; methanol%: 40%-40%; flow rate: 2.8 mL/min.
- the system was slowly warmed to room temperature (25 °C), and then allowed to react at room temperature (25 °C) for 16 h.
- the system was poured into ice water, and dichloromethane (100 mL) was added to the resulting mixture.
- the system was alkalified with 2 M aqueous solution of sodium hydroxide and then separated into layers.
- the organic phase was dried over anhydrous sodium sulfate and filtered.
- the filtrate was concentrated to obtain a crude product
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Phenomenex Gemini-NX 80 ⁇ 30 mm ⁇ 3 ⁇ m; mobile phase: [water (10 mM ammonium bicarbonate solution)-acetonitrile]; acetonitrile%: 29%-59% 9.5 min) to obtain compounds 14A and 14B.
- Separation condi ions chromatographic column: WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0688% trifluoroacetic acid solution)-acetonitrile (0.0625% trifluoroacetic acid solution); acetonitrile: 5%-95% 0.7 min, 95% 0.4 min; flow rate: 1.5 mL/min.
- chromatographic column WELCH Ultimate LP-C18 150 ⁇ 4.6 mm 5 ⁇ m; column temperature: 40 °C; mobile phase: water (0.0375% trifluoroacetic acid solution)-acetonitrile (0.01875% trifluoroacetic acid solution); acetonitrile: 5%-95% 0.7 min, 95% 0.4 min; flow rate: 1.5 mL/min.
- the resulting solution was adjusted to about pH 10 with 1 N sodium hydroxide.
- the aqueous phase was adjusted to about pH 3 with 6 M hydrochloric acid and extracted with ethyl acetate (50 mL ⁇ 3).
- the organic phases were pooled, washed with saturated brine (50 mL ⁇ 3), dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to obtain compound 15-3.
- Cuprous chloride (13.52 g, 136.57 mmol, 3.27 mL) and tert-butyl nitrite (12.80 g, 124.13 mmol, 14.76 mL) were dissolved in acetonitrile (150 mL), and compound 15-3 (16 g, 83.72 mmol) was added to the resulting solution at 60 °C.
- the system was stirred at 60 °C for 20 min under a nitrogen atmosphere.
- the system was cooled to room temperature (25 °C), and hydrochloric acid (4.32 mol, 273 mL, 15% purity) was added thereto.
- the system was stirred at room temperature (25 °C) for 1 h.
- Diisopropylethylamine (1.23 g, 9.50 mmol, 1.65 mL) was dissolved in anhydrous tetrahydrofuran (60 mL), and n -butyllithium (2.5 M, 57.00 mL) was added dropwise to the resulting solution at -78 °C. After the completion of the addition, the system was stirred at-30 °C for 10 min. The system was cooled to -78 °C, and a solution of compound 15-4 (10 g, 47.50 mmol) in tetrahydrofuran (60 mL) was added thereto. After the completion of the addition, the system was stirred at -78 °C for 4 h.
- the organic phases were pooled, dried over anhydrous sodium sulfate, and filtered. The filtrate was concentrated to obtain a crude product.
- the crude product was purified by preparative high performance liquid chromatography (separation conditions: chromatographic column: Xtimate C18 150 ⁇ 40 mm ⁇ 5 ⁇ m; mobile phase: water (10 mM ammonium bicarbonate)-acetonitrile; acetonitrile: 30%-50% 8 min) to obtain compound 15-5.
- Step 13 Preparation of compounds 15A, 15B, 15C and 15D
- chromatographic column Chiralcel OD-3 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: CO 2 -ethanol (0.05% DEA), ethanol%: 5%-40% 4 min, 40% 2.5 min, 5% 1.5 min; flow rate: 2.8 mL/min.
- chromatographic column Chiralcel OD-3 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: CO 2 -ethanol (0.05% DEA), ethanol%: 5%-40% 4 min, 40% 2.5 min, 5% 1.5 min; flow rate: 2.8 mL/min.
- chromatographic column Chiralcel OD-3 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: CO 2 -ethanol (0.05% DEA), ethanol%: 5%-40% 4 min, 40% 2.5 min, 5% 1.5 min; flow rate: 2.8 mL/min.
- chromatographic column Chiralcel OD-3 100 ⁇ 4.6 mm I.D., 3 ⁇ m; column temperature: 35 °C; mobile phase: CO 2 -ethanol (0.05% DEA), ethanol%: 5%-40% 4 min, 40% 2.5 min, 5% 1.5 min; flow rate: 2.8 mL/min.
- NCI-H358 cells ATCC accession No. CRL-5807 expressing mutant RAS (G12C) were cultured in an RPMI medium containing 10% fetal bovine serum, penicillin/streptomycin double antibody. The cells were plated on to a 96-well plate (Coming Cat. No. 3699) at 40,000 cells per well and left standing overnight to adhere to the bottom of the plate. The cells were treated with or without the compounds of the present disclosure (dimethylsulfoxide, DMSO), with a final DMSO concentration of 0.5% ensured.
- DMSO dimethylsulfoxide
- the ERK phosphorylation level was detected by enzyme-linked immunosorbent assay (ELISA).
- a phospho-ERK antibody (Cell Signal Technology Cat. No. 4370) was 1:400 diluted with 1 ⁇ blocking solution containing 0.05% tween 20 and added to the 96-well plate for overnight incubation at 4 °C. The plate was washed 5 times with PBS containing 0.05% tween 20.
- An HRP-conjugated secondary antibody (Thermo Cat. No. 31460) was 1:10,000 diluted with 1 ⁇ blocking buffer containing 0.05% tween 20 and added to the 96-well plate for incubation for 2 h at room temperature.
- the plate was washed 5 times with PBS containing 0.05% Tween and incubated with TMB (Thermo Cat. No. 4816) at room temperature for 15 min.
- the reaction was terminated with 1 mol/L H 2 SO 4 , and the OD values were read at a wavelength of 450 nm using an EnVision (PerkinElmer).
- the total number of cells per well was measured using the Janus green staining method.
- the 96-well plate after the detection of the ERK phosphorylation level was washed to colorlessness with PBS and incubated with 0.1% Janus green (Abcam Cat. No. ab111622) for 10 min. After washing with double distilled water, the plate was incubated with 0.1 mol/L HCl with shaking for 10 min. The OD values were read at a wavelength of 595 nm using an EnVision (PerkinElmer).
- the compounds of the present disclosure exhibited excellent ability to inhibit RAS-mediated signaling.
- the ability of the compounds of the present disclosure to inhibit the growth of KRAS-G12C-expressing cells was assessed by measuring cell viability and calculating GI50 values.
- Tumor cell line NCI-H358 (ATCC accession No. CRL-5807) expressing KRAS-G12C was cultured in an RPMI medium supplemented with 10% fetal bovine serum and penicillin/streptomycin double antibody; tumor cell line MIA PaCa2 (ATCC CRL-1420) expressing KRAS-G12C was cultured in a DMEM medium supplemented with 10% fetal bovine serum, 2.5% horse serum and penicillin/streptomycin double antibody.
- NCI-H358 and MIA-Paca2 cells were seeded on a black clear-bottom 384-well plate (PerkinElmer Cat. No. 6007460) at densities of 1000 and 800 cells, respectively, and allowed to adhere to the walls overnight (8-12 h).
- the diluted compounds of the present disclosure with a concentration that was 5 times that of the working solution (containing 0.1% dimethyl sulfoxide, namely DMSO, at the final concentration) was added to the experimental groups; the same dilution (containing 0.1% DMSO at the final concentration) as in the experimental groups was added to the control group.
- the amount of cell proliferation was measured by measuring ATP content using a Cell Titer Glo reagent (Promega Cat.
- Steps are briefly as follows: the cell plate was taken out and equilibrated at normal temperature for 30 min; the Cell Titer Glo reagent with an equal volume to that of the culture was added; the culture plate was placed on a shaker for lysis for 2 min; the culture plate was left standing at normal temperature for 10 min; the optical signal values were read using a microplate reader EnVision (PerkinElmer).
- GI 50 (NCI-H358, ⁇ M) GI 50 (MIA-Paca2, ⁇ M) ARS-1620 0.510 1.209 Compound 1B 0.084 0.296 Compound 2A 0.029 0.048 Compound 2B 1.116 15.993 Compound 4A 0.034 0.324 Compound 6B 0.066 0.297 Compound 7A 0.133 0.629 Compound 7B 0.080 0.200 Compound 8A 0.350 0.304 Compound 10A 0.111 0.333 Compound 10B 0.155 0.403 Compound 11A 0.070 0.685 Compound 12A 0.010 0.054 Compound 13 0.320 1.273 Compound 15A 3.428 4.522 Compound 15C 0.060 0.087 Compound 15D 0.959 1.099
- the compounds of the present disclosure have excellent inhibitory activity against the proliferation of NCI-H358 and MIA-Paca2 cells.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910712388 | 2019-08-02 | ||
CN201910899724 | 2019-09-23 | ||
CN201911157922 | 2019-11-22 | ||
CN202010054185 | 2020-01-17 | ||
PCT/CN2020/106573 WO2021023154A1 (zh) | 2019-08-02 | 2020-08-03 | 四并环类化合物及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP4011886A1 true EP4011886A1 (de) | 2022-06-15 |
EP4011886A4 EP4011886A4 (de) | 2023-07-26 |
Family
ID=74483410
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP20850862.2A Pending EP4011886A4 (de) | 2019-08-02 | 2020-08-03 | Tetrazyklische verbindung, herstellungsverfahren dafür und verwendung da |
Country Status (10)
Country | Link |
---|---|
US (1) | US20220298174A1 (de) |
EP (1) | EP4011886A4 (de) |
JP (1) | JP2022543767A (de) |
KR (1) | KR20220079521A (de) |
CN (1) | CN112300195B (de) |
AU (1) | AU2020325477A1 (de) |
CA (1) | CA3149403A1 (de) |
IL (1) | IL290276A (de) |
MX (1) | MX2022001421A (de) |
WO (1) | WO2021023154A1 (de) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019217307A1 (en) | 2018-05-07 | 2019-11-14 | Mirati Therapeutics, Inc. | Kras g12c inhibitors |
JP2022517222A (ja) | 2019-01-10 | 2022-03-07 | ミラティ セラピューティクス, インコーポレイテッド | Kras g12c阻害剤 |
CN112390818B (zh) * | 2019-08-12 | 2023-08-22 | 劲方医药科技(上海)有限公司 | 取代的杂芳环并二氢嘧啶酮衍生物,其制法与医药上的用途 |
US11453683B1 (en) | 2019-08-29 | 2022-09-27 | Mirati Therapeutics, Inc. | KRas G12D inhibitors |
JP2022548791A (ja) | 2019-09-24 | 2022-11-21 | ミラティ セラピューティクス, インコーポレイテッド | 組み合わせ療法 |
EP4051678A1 (de) | 2019-10-28 | 2022-09-07 | Merck Sharp & Dohme Corp. | Kleinmolekülige inhibitoren der kras-g12c-mutante |
WO2021127429A1 (en) | 2019-12-20 | 2021-06-24 | Mirati Therapeutics, Inc. | Sos1 inhibitors |
CN113929676A (zh) * | 2020-07-14 | 2022-01-14 | 浙江海正药业股份有限公司 | 吡啶并杂环类衍生物及其制备方法和用途 |
CN113980032B (zh) * | 2020-07-27 | 2023-06-16 | 江苏恒瑞医药股份有限公司 | 稠合四环类衍生物、其制备方法及其在医药上的应用 |
CN117083280A (zh) * | 2021-03-07 | 2023-11-17 | 北京加科思新药研发有限公司 | Kras g12d抑制剂的稠环衍生物 |
CA3226720A1 (en) * | 2021-07-23 | 2023-01-26 | Suzhou Zanrong Pharma Limited | Kras g12d inhibitors and uses thereof |
KR20240055778A (ko) | 2021-09-01 | 2024-04-29 | 노파르티스 아게 | Tead 억제제를 포함하는 제약 조합물 및 암의 치료를 위한 이의 용도 |
WO2023046135A1 (en) * | 2021-09-27 | 2023-03-30 | Jacobio Pharmaceuticals Co., Ltd. | Polycyclic fused ring derivatives and use thereof |
CA3234517A1 (en) * | 2021-10-22 | 2023-04-27 | Xin Li | Nitrogen-containing tetracyclic compound, preparation method therefor, and medical use thereof |
WO2023072297A1 (zh) * | 2021-11-01 | 2023-05-04 | 江苏恒瑞医药股份有限公司 | 含氮的四环化合物、其制备方法及其在医药上的应用 |
WO2023103906A1 (zh) * | 2021-12-07 | 2023-06-15 | 贝达药业股份有限公司 | Kras g12d抑制剂及其在医药上的应用 |
WO2024002024A1 (zh) * | 2022-06-27 | 2024-01-04 | 石药集团中奇制药技术(石家庄)有限公司 | 三环类化合物及其用途 |
WO2024032703A1 (en) * | 2022-08-11 | 2024-02-15 | Beigene, Ltd. | Heterocyclic compounds, compositions thereof, and methods of treatment therewith |
WO2024081674A1 (en) | 2022-10-11 | 2024-04-18 | Aadi Bioscience, Inc. | Combination therapies for the treatment of cancer |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6441910B2 (ja) * | 2013-09-30 | 2018-12-19 | シャンハイ インリ ファーマシューティカル カンパニー リミティド | 縮合ピリミジン化合物、中間体、その調製方法、組成物及び使用 |
JOP20190154B1 (ar) * | 2016-12-22 | 2022-09-15 | Amgen Inc | بنز أيزو ثيازول، أيزو ثيازولو [3، 4-b] بيريدين، كينازولين، فثالازين، بيريدو [2، 3-d] بيريدازين ومشتقات بيريدو [2، 3-d] بيريميدين على هيئة مثبطات kras g12c لمعالجة سرطان الرئة، أو سرطان البنكرياس أو سرطان القولون والمستقيم |
TW201906848A (zh) * | 2017-05-11 | 2019-02-16 | 瑞典商阿斯特捷利康公司 | 化學化合物 |
JOP20190272A1 (ar) * | 2017-05-22 | 2019-11-21 | Amgen Inc | مثبطات kras g12c وطرق لاستخدامها |
ES2928576T3 (es) * | 2017-09-08 | 2022-11-21 | Amgen Inc | Inhibidores de KRAS G12C y métodos de uso de los mismos |
EA202091186A1 (ru) * | 2017-11-15 | 2020-10-01 | Мирати Терапьютикс, Инк. | ИНГИБИТОРЫ KRas G12C |
KR20220012255A (ko) * | 2019-04-28 | 2022-02-03 | 젠플리트 테라퓨틱스 (상하이) 아이엔씨. | 옥사아자퀴나졸린-7(8h)-케톤 화합물, 이의 제조 방법 및 이의 약학적 응용 |
CN112390818B (zh) * | 2019-08-12 | 2023-08-22 | 劲方医药科技(上海)有限公司 | 取代的杂芳环并二氢嘧啶酮衍生物,其制法与医药上的用途 |
-
2020
- 2020-08-03 US US17/632,167 patent/US20220298174A1/en active Pending
- 2020-08-03 KR KR1020227006959A patent/KR20220079521A/ko active Search and Examination
- 2020-08-03 JP JP2022506075A patent/JP2022543767A/ja active Pending
- 2020-08-03 AU AU2020325477A patent/AU2020325477A1/en active Pending
- 2020-08-03 WO PCT/CN2020/106573 patent/WO2021023154A1/zh unknown
- 2020-08-03 MX MX2022001421A patent/MX2022001421A/es unknown
- 2020-08-03 CN CN202010769535.0A patent/CN112300195B/zh active Active
- 2020-08-03 CA CA3149403A patent/CA3149403A1/en active Pending
- 2020-08-03 EP EP20850862.2A patent/EP4011886A4/de active Pending
-
2022
- 2022-02-01 IL IL290276A patent/IL290276A/en unknown
Also Published As
Publication number | Publication date |
---|---|
IL290276A (en) | 2022-04-01 |
WO2021023154A1 (zh) | 2021-02-11 |
CN112300195B (zh) | 2021-12-24 |
JP2022543767A (ja) | 2022-10-14 |
AU2020325477A1 (en) | 2022-03-17 |
US20220298174A1 (en) | 2022-09-22 |
EP4011886A4 (de) | 2023-07-26 |
CA3149403A1 (en) | 2021-02-11 |
MX2022001421A (es) | 2022-06-08 |
KR20220079521A (ko) | 2022-06-13 |
CN112300195A (zh) | 2021-02-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP4011886A1 (de) | Tetrazyklische verbindung, herstellungsverfahren dafür und verwendung da | |
EP4043464A1 (de) | Kondensierte pyridonverbindung, herstellungsverfahren dafür und verwendung davon | |
US9884861B2 (en) | Compositions useful for treating disorders related to kit | |
US9688680B2 (en) | Compositions useful for treating disorders related to kit | |
EP3746424B1 (de) | Erbb/btk-inhibitoren | |
EP3057969B1 (de) | Zusammensetzungen zur behandlung von erkrankungen verbunden mit kit | |
AU2014256635B2 (en) | Deuterated diaminopyrimidine compounds and pharmaceutical compositions comprising such compounds | |
AU2019392231B2 (en) | Isoindoline compound, and preparation method, pharmaceutical composition, and application of isoindoline compound | |
EP3919483A1 (de) | Benzopyridone heterocyclic compound and use thereof | |
CN110036014A (zh) | 一种具有axl抑制活性的化合物及其制备和应用 | |
EP3466955A1 (de) | Verfahren zur herstellung von oxazolo[4,5-b]pyridin- und thiazolo[4,5-b]pyridin-derivaten als irak4-inhibitoren zur behandlung von krebs | |
EP3344613B1 (de) | Arylverbindungen und ihre verwendung als therapeutische wirkstoffe | |
JP2017508779A (ja) | ROS1阻害剤としての置換4,5,6,7−テトラヒドロ−ピラゾロ[1,5−α]ピラジン誘導体および5,6,7,8−テトラヒドロ−4H−ピラゾロ[1,5−α][1,4]ジアゼピン誘導体 | |
US20160297815A1 (en) | 7-azaindole or 4,7-diazaindole derivatives as ikk epsilon and tbk1 inhibitor and pharmaceutical composition comprising same | |
CN114163457A (zh) | 嘧啶并五元氮杂环化合物及其用途 | |
EP4289852A1 (de) | 5,6-dihydrothieno[3,4-h chinazolin-verbindung | |
CN112898314A (zh) | 去泛素化酶抑制剂的制备与应用 | |
CN115536656A (zh) | 作为hpk1抑制剂的杂环化合物 | |
KR102485731B1 (ko) | Jak 효소 억제제 및 이의 제조 방법과 용도 | |
EP3766883B1 (de) | Imidaxopyrolonverbindung und anwendung davon | |
CN114075196A (zh) | 一类芳香环并内酰胺类化合物、其制备方法和用途 | |
US20230227472A1 (en) | Fused pyridone compound, and preparation method therefor and use thereof | |
US20230293535A1 (en) | Erbb/btk inhibitors | |
CN114591319A (zh) | 四氢吡啶并嘧啶类衍生物及其用途 | |
CN116969976A (zh) | 去泛素化酶抑制剂及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20220302 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20230626 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: A61K 31/519 20060101ALI20230620BHEP Ipc: A61P 35/00 20060101ALI20230620BHEP Ipc: C07D 498/22 20060101ALI20230620BHEP Ipc: C07D 498/14 20060101AFI20230620BHEP |