EP3764813A1 - Production d'extraits de vanille sans éthanol - Google Patents

Production d'extraits de vanille sans éthanol

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Publication number
EP3764813A1
EP3764813A1 EP18712136.3A EP18712136A EP3764813A1 EP 3764813 A1 EP3764813 A1 EP 3764813A1 EP 18712136 A EP18712136 A EP 18712136A EP 3764813 A1 EP3764813 A1 EP 3764813A1
Authority
EP
European Patent Office
Prior art keywords
vanilla
ethanol
extraction
extract
extraction solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18712136.3A
Other languages
German (de)
English (en)
Inventor
Johannes KIEFL
Stefan Brennecke
Martin HEINEMEYER
Dominik Winkler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Symrise AG
Original Assignee
Symrise AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symrise AG filed Critical Symrise AG
Publication of EP3764813A1 publication Critical patent/EP3764813A1/fr
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/11—Natural spices, flavouring agents or condiments; Extracts thereof obtained by solvent extraction
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A23L27/115—Natural spices, flavouring agents or condiments; Extracts thereof obtained by distilling, stripping, or recovering of volatiles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

Definitions

  • the present invention relates to a method for producing an ethanol-free vanilla extract, an ethanol-free vanilla extract, which is obtainable by the process according to the invention, an ethanol-free vanilla extract comprising at most 100 mg / kg of ethanol, the use of the ethanol-free vanilla extract and products containing the ethanol-free vanilla extract.
  • the focus of the present invention is to provide a process for producing an ethanol-free vanilla extract without the use of ethanol and to provide an ethanol-free vanilla extract having only traces of ethanol naturally contained in fermented vanilla pods and prepared without the use of ethanol.
  • the vanilla is a spice that is obtained from the fermented capsule fruits of various species of the orchid genus Vanilla.
  • the spice vanilla Vinilla planifolia
  • the Tahiti vanilla Vanilla tahitensis
  • the Guadeloupe vanilla Vanilla pompona
  • vanilla planifolia The aroma of the dried and fermented pods of Vanilla planifolia, Vanilla tahitensis and Vanilla pompona is one of the highest quality and the world's most widely used flavors. It is used for the flavoring of numerous foods such as ice cream, dairy products, desserts, chocolate products, baked goods, spirits, etc.
  • Vanilla pods contain on average about 1, 6 to 2.4% of the flavoring agent vanillin, which makes an important contribution to the flavor impression, in addition to other flavorings. Extracts from the above vanilla pods are preferred because of their complex and authentic flavor profile the vanillin based extract-free flavors.
  • Fermented vanilla beans are extracted for decades with ethanol as extraction solvent.
  • the extract thus obtained is subsequently concentrated by distillation. This process and storage-related changes of the sensory profile occur.
  • vanilla extracts The odor impression of vanilla extracts is caused by more than 30 odorous substances such as capric acid, methyl cinnamate, guaiacol, 4-methyl guaiacol, ethyl linolenate, p-hydroxybenzoic acid, p-hydroxybenzaldehyde and vanillin (Perez-Silva et al., Food Chemistry 99 , 2006, 728-735).
  • vanilla extracts are caused by numerous non-volatile substances.
  • Schwarz and Flofmann et al. more than 10 flavors such as, for example, 4- (4-hydroxybenzyl) -2-methoxyphenol, divanillin, (1-0-vanilloyl) - (6-0-feruloyl) - ⁇ -D-glucopyranoside, many of which are sensorially active are unknown (Schwarz and Hofmann, J. Agric. Food Chem., 57, 2009, 3729-3737).
  • vanilla extract Since numerous secondary components that are not sensitive to the sensory, such as polysaccharides, waxes, fats in the extraction of fermented vanilla pods stabilization of the vanilla extract plays an important role. Normally, ethanol remains in the extract as a solvent after extraction and thus stabilizes waxes, fats and other lipophilic substances that would precipitate without the use of a solubilizer.
  • vanilla extract dissolved in ethanol changes its sensory profile during storage and develops, for example, in the language of the expert, fruity, reminiscent of rum notes.
  • WO 2006 047404 A2 discloses, for example, the fermentation of any plant starting material with a glucosidase, followed by an aqueous extraction in the presence of another reactant, for example an amino acid or a sugar. Both work neither teaches a teaching on the stabilization of the microbiologically unstable water extract nor a method for the concentration of the extract.
  • a first subject of the present invention relates to a process for the preparation of an ethanol-free vanilla extract, which comprises the following steps:
  • Another object of the present invention relates to an ethanol-free vanilla extract, prepared or prepared by the above process according to the invention.
  • the present invention relates to an ethanol-free vanilla extract comprising at most 100 mg / kg of ethanol.
  • Another aspect of the present invention relates to the use of the ethanol-free vanilla extract for the preparation, in particular for flavoring or reconstitution of flavor, food, semi-luxury foods, beverage products, semi-finished products, hygiene products, cosmetic or pharmaceutical products, tobacco products and products for animal nutrition.
  • the present invention relates to food, semi-luxury foods, beverage products, semi-finished products, hygiene products, cosmetic or pharmaceutical products, tobacco products and products for animal nutrition, which comprise the ethanol-free vanilla extract according to the invention.
  • Figure 1 is a representation of sensor profiles of extracts of fermented vanilla pods, which were prepared by the process according to the invention.
  • Figure 2 is a graph of vanillin concentrations and soluble extract levels of acetone extracts and methanol extracts of fermented vanilla pods made by the process of the present invention. Vanillin contents (black bars, indication in percent) and soluble extract contents (gray bars, indication in percent) of acetone and methanol extracts of fermented vanilla pods according to the method according to the invention.
  • Figure 3 is a representation of sensor profiles of extracts of fermented vanilla pods, which were prepared by the process according to the invention, depending on the extraction temperature.
  • Figure 4 is a representation of vanillin yields in a consecutive extraction with acetone and methanol.
  • the solid line describes the vanillin content of an extraction with acetone
  • the dashed line describes the vanillin content of an extraction with methanol.
  • Figure 5 is a representation of the vanillin yield in a consecutive extraction with methanol at different temperatures.
  • the solid line describes the vanillin content of an extraction at 25 ° C
  • the dotted line describes the vanillin content of an extraction at 65 ° C.
  • ethanol-free vanilla extract is understood to mean a vanilla extract which, with the exception of traces of ethanol naturally present in fermented vanilla pods, has no ethanol and is prepared without the use of ethanol.
  • fermented vanilla pods are provided in a first step.
  • the vanilla pods are fermented vanilla pods of the genus Vanilla planifolia, Vanilla tahitensis and Vanilla pompona.
  • the fermented vanilla pods are prepared as follows: Vanilla plants are grown in plantations. The up to 30 cm long vanilla pods, botanically correct are capsule fruits, are harvested shortly before maturity when they are yellowish-green. The green fruits do not have the typical flavor and taste of the finished product at harvest time.
  • the fruits must first be subjected to fermentation. For this it is first necessary that the cells of the capsule fruits are lysed. This is done by conventional methods known in the art, for example by physical methods such as by treating the green vanilla pods with hot water or steam, freezing and thawing the green vanilla pods, by treating the green vanilla pods with CO2 under pressure or by enzymatic treatment of the green vanilla pods with cellulase, pectinases, etc. In this process step, on the one hand a release of glucovanillin, the precursor of vanillin, and on the other hand, a release of endogenously occurring in the green vanilla pods ⁇ -glucosidases.
  • vanilla pods a fermentation and drying of the vanilla pods takes place during which they lose water and assume their typical dark brown color.
  • the glucovanillin is converted into vanillin, the main flavoring agent of vanilla, by hydrolysis.
  • the fermented vanilla beans thus produced usually have a moisture content of 10 to 30% and a vanillin content of 1 to 3%, based on the dry matter.
  • the fermented vanilla pods described above, for use in the process of the present invention are then shredded into small pieces about 10 mm in size, for example cut, chopped, cut, by means of cutting mills or shredders known in the art and known to those skilled in the art shredded, crushed or chopped. By using crushed vanilla pods, the subsequent extraction is facilitated.
  • the comminuted vanilla pod particles have a size of 1 to 10 mm.
  • the vanilla pods are too pasty and make the subsequent process steps, such as filtration (clogging of the sieves) or distillation (bumping), more difficult.
  • Pretreatment of the vanilla pods is not required in the process of the invention.
  • vanilla pods bits are transferred, for example, in a Willmes press and to ensure circulation to the contact between the extraction material and the extraction solvent or extraction solvent mixture and thus to increase the efficiency of the extraction.
  • an ethanol-free vanilla extract according to the present invention the use of ethanol having a log P value of -0.33 is excluded as extraction solvent as such or in an extraction solvent mixture of the aforementioned definition ,
  • Extraction is a physical process in which a starting material, wholly or at the surface, is exposed for some time to the action of a liquid, such as water, oil or a solvent, which serves as a solvent for certain ingredients and which Extract ingredients from the solid.
  • a liquid such as water, oil or a solvent
  • the starting material is not dissolved as such, but only soluble constituents of which go into the solvent.
  • the choice of solvent depends on their respective dissolving power with respect to the substance to be dissolved.
  • logP logP
  • C (n-octanol) is the concentration of the solvent in the n-octanol phase; and C (water) is the concentration of the solvent in the water phase.
  • K (n-octanol / water) is a dimensionless distribution coefficient indicating the ratio of the concentration of the solvent in a two-phase system of n-octanol and water. It is thus a model measure of the polarity or water / fat solubility of the solvent.
  • the log P value is a common physical parameter and is positive for lipophilic solvents and negative for hydrophilic solvents. Ultimately, the log P value of the extractant determines which flavorants are extracted qualitatively and quantitatively from the shredded vanilla bean particles.
  • An extraction solvent mixture according to the present invention comprises at least one of the above-mentioned. organic extraction solvent in combination with another from the group of the above-mentioned organic extraction solvents.
  • an extraction solvent mixture comprises at least one of the above-mentioned. organic extraction solvent in admixture with water.
  • an extraction solvent mixture according to the present invention comprises at least one of the above-mentioned. organic extraction solvent in admixture with another organic solvent which is typically used as an ingredient of foods, for example vegetable oils, essential oils, sugar syrups, etc.
  • vegetable oils are selected from the group consisting of sunflower, soybean, rapeseed , Peanut, palm, wheat germ, corn germ, olive and linseed oil.
  • an extraction solvent mixture containing at least one of the o.g. organic extraction solvent is used, the mixing ratio of organic extraction solvent to another organic solvent in a range from 10: 90 (v / v) to 90: 10 (v / v), preferably in a ratio of 50 : 50 (v / v).
  • the mixing ratio of organic extraction solvent to water is in a range of 90:10 (v / v) to 60:40 (v / v), preferably in a ratio of 80 : 20 (v / v).
  • the above-mentioned extraction solvents are used neat. Surprisingly, it was found that the extraction of the comminuted vanilla pods in step (c) of the method according to the invention with acetone, which has a log P value - 0.24, with respect to the sensory relevant ingredients, ie the flavoring flavoring in the Particularly good results compared to the other above-mentioned extraction solvents.
  • the ratio of the amount of extraction solvent or extraction solvent mixture to vanilla seed quantity used is important for exhaustive extraction.
  • saturation of the extraction solvent or of the extraction / solvent mixture rapidly occurs, with the result that the valuable constituents from the comminuted vanilla pods can not be completely extracted.
  • the extraction solvent or extraction solvent mixture is therefore used in the inventive method in excess of the amount of comminuted vanilla pods to ensure an exhaustive extraction.
  • the extraction solvent mixture is added to the amount of shredded vanilla pods in a range of 1: 1 (w / w) to 40: 1 (w / w) used.
  • the ratio of extraction solvent / extraction solvent mixture to the amount of shredded vanilla pods is 10: 1 (w / w).
  • the extraction of the comminuted vanilla pods in the process of the invention is carried out in a defined time / temperature profile. Surprisingly, it has been found that in an extraction at room temperature, for example, sweet, malty-smelling flavors develop increasingly. With an extraction temperature at higher temperatures of about 65 ° C increases the solubility and thus the yield of the flavorings to be extracted, while at the same time arise dry fruit notes.
  • the extraction of the crushed fermented vanilla pods is carried out in a temperature range of 20 to 60 ° C (preferably 30 to 60 ° C) for a period of 8 to 20 hours.
  • Prolonged extraction time may result in the degradation of valuable ingredients, the formation of dry fruit notes, volatile carotenoid degradation products such as damascenones, and yield aldehydes, which in turn results in the distribution of the aroma components in the vanilla extract, and thus the Sensory profile of the vanilla extract influenced.
  • the extraction is carried out at a temperature of 40 ° C for a total of 16 hours.
  • the extraction of the comminuted vanilla pods is carried out in such a way that the extraction solvent or the extraction solvent mixture is renewed at least twice but not more than five times consecutively.
  • the extraction solvent or the extraction / solvent mixture is renewed at least three times consecutively. Consecutive extraction ensures exhaustive extraction of the flavorings from the crushed vanilla pods, maximizing the yield of flavorings.
  • the crude extract obtained is decanted from the extraction material and the fractions obtained from several consecutive extraction steps crude extracts are combined to form a crude extract.
  • the extraction solvent or extraction solvent mixture used in the process according to the invention must be completely removed from the crude extract either for sensory reasons, for example due to a strong intrinsic odor, or due to regulatory requirements.
  • acetone itself is an odorant with a threshold of 300 mg / kg, which adversely affects the sensory profile of the flavor extract produced with it with its fingernail-like odor.
  • the European Union is calling for the removal of acetone to the technically unavoidable level, and the IOFI (International Organization for the Flavor Industry) even requires a limit of 2 mg / kg in the final application in its "Code of Practice". It is a set of rules that is used internationally in the flavor industry.
  • ethanol is an odorant with a threshold of 1,000 mg / kg, which is not perceived olfactorily even at one percent by volume in the end-use application, and which may be used indefinitely in foods.
  • a solvent having a boiling point which is preferably at least 20 ° C higher than the boiling point of the extraction solvent used in step (c) of the inventive method or at least one organic Extraction solvent in the extraction solvent mixture used.
  • the protective solvent is a solvent selected from the group consisting of 1, 2-propanediol, 1, 3-propanediol, diacetin, triacetin, triethyl citrate and solubilizing foods such as vegetable oils.
  • the extraction solvent or extraction / solvent mixture used for the extraction of the comminuted vanilla pods and the protective solvent used in step (c) of the process according to the invention have distinctly different boiling points, no azeotrope is formed on evaporation or distilling off, and the extraction Solvent or the extraction-solvent mixture can be removed cleanly and completely from the crude extract.
  • the use of 1, 2-propanediol, the evaporation or distilling off the extraction solvent or the extraction solvent mixture of the crude extract with a residual amount of less than 1,000 mg / kg at a vacuum below 10 mbar particularly allows significant loss of flavoring aroma components of the crude extract.
  • 2-Propanediol also known as propylene glycol
  • 2-Propandiol belongs to the polyhydric alkanols and is chiral at C2, so there is an (R) - enantiomer and an (S) -enantiomer.
  • 2-Propandiol is approved in the EU as a food additive.
  • 1, 2-propanediol is used as a carrier.
  • 1, 2-propanediol can be used as a racemate, as R or S enantiomer or as any mixture of its enantiomers.
  • the ratio of the amount of vanilla bean seed used to the amount of protective solvent is crucial.
  • a very concentrated vanilla extract is obtained.
  • a saturation of the protective solvent phase occurs more quickly with valency-adding flavors, with the result that constituent components are already lost during the evaporation of the extraction solvent or of the extraction-solvent mixture.
  • an excessive amount of protective solvent is not desirable because the extract is then very diluted and smells only slightly.
  • the ratio of vanillot seed amount used to the amount of protective solvent to which the crude extract is added ranges from 1:10 (w / w) to 10: 1 (w / w).
  • a ratio of vanillate seed amount used to the amount of protective solvent added in a range of 1: 1 (w / w) to 4: 1 (w / w) prior to removal of the extraction solvent or the extraction solvent mixture of the crude extract has been found to be particularly advantageous.
  • the crude extract mixed with the protective solvent is filtered.
  • the filtration of the crude extract can be carried out before the addition of the protective solvent.
  • the filtration is carried out by means of suitable commercially available filters, preferably via plate filter with 2.9 mm thick cellulose plates having a water permeability of 10200 l / m 2 / min and exclusion of 700 g / m 2 , to solid, non-soluble components from the vanilla extraction remove.
  • the filtered crude extract thus obtained is then placed in a suitable distillation bubble.
  • the extraction solvent or the extraction / solvent mixture is removed from the crude extract by distillation.
  • the evaporation or distilling off the extraction solvent or the extraction solvent mixture from the crude extract takes place gently and close to the boiling point of the extraction solvent or the extraction solvent mixture.
  • the evaporation of the extraction solvent or of the extraction / solvent mixture at a temperature of 40 ° C. and / or a vacuum of 100 mbar has proved to be particularly advantageous.
  • the crude extract is first freed from> 99% of the extraction solvent or the extraction solvent mixture.
  • the distillation in the inventive method is preferably in a Falling film evaporator, rotary evaporator or thin-film evaporator or carried out by means of a spinning cone process.
  • solutions or solvent mixtures are evaporated in a thin film. Due to a high heat transfer coefficient, rapid and efficient evaporation of the extraction solvent or of the extraction / solvent mixture takes place. Since the devices can be operated very well in a vacuum, they are suitable for the gentle distillation of solution mixtures at low temperatures.
  • the removal of the extraction solvent or the extraction solvent mixture is carried out in a spinning cone process. It is a rectification column with rotating conical inserts. On the underside of the cones ribs are attached, which provide for turbulence in the gas phase and thus an intensive mass transfer between liquid and vapor. Since the working temperatures are very mild, heat damage to the distilled material is avoided.
  • the vacuum is reduced to 20 mbar in the distillation, in order to reduce the remaining amounts of extraction solvent and / or water to less than 1,000 mg / kg to remove from the vanilla extract thus obtained.
  • step (c) of the process according to the invention depending on the polarity of the extraction solvent used or the extraction solvent mixture used - in addition to the value-giving flavors also numerous secondary components that are not sensitive to sensor , such as waxes and fats, co-extracted. Therefore, the stabilization of the vanilla extract plays an important role.
  • vanilla extracts prepared by the prior art using ethanol ethanol remains in the vanilla extract as a solvent after extraction and stabilizes waxes and other lipophilic substances which would cause solubility problems and fail without the use of a solubilizer.
  • the vanilla extract is freed from the lipophilic wax-containing phase.
  • the vanilla extract freed from the extraction solvent or extraction solvent mixture is allowed to stand simply at a temperature in the range from 10 to 40 ° C., or from 20 to 40 ° C., preferably at a temperature of 30 ° C. It finds in the vanilla extract during a settling time of 10 to 48 hours, a phase separation in a Wax phase and a liquid vanilla extract phase instead. During this time, the transition of flavoring flavorants into the wax phase is limited and the time of phase separation optimal, as can be seen from Table 1 below.
  • the wax phase is separated from the liquid vanilla extract phase by draining the liquid vanilla extract phase from the distillation bubble. The wax phase is discarded.
  • Table 1 Selected phenols and fatty acids in the wax phase and the dewaxed acetone-vanilla extract according to Example 1 (SBSE-GCMS)
  • the results of the analysis in Table 1 show that only traces of valuable phenols are detectable in the wax phase.
  • the wax phase is characterized by fatty acids and their glycerides and fatty alcohol esters.
  • the results listed in Table 1 were obtained by means of SBSE-GCMS from the acetone extract, which was prepared according to Example 1 described below.
  • SBSE Stir Bar Sorptive Extraction
  • the method is based on the use of a sorbent-coated magnetic stir bar, which mixes the sample to be analyzed (de-waxed vanilla extract or wax phase), wherein the analytes accumulate in the shell material.
  • 1,2-propanediol is antimicrobially active at a concentration of> 1%
  • the vanilla extract is both microbiologically stable and protected against oxidation.
  • vanilla extract in 1,2-propanediol does not result in any change in the odor type, whereas storage in, for example, ethanol produces fruity, rum-like notes.
  • the extracts were prepared as follows: In a 300 ml Erlenmeyer flask with magnetic stirrer were each 37.5 g of crushed vanilla pods with 150 g of acetone or 150 g of ethanol or 150 g of methanol or 150 g of 2-propanol or 150 ethyl acetate or a Extracted mixture of 65% methanol / 35% water as extraction solvent for 2 h. Before the distillation, the respective crude extracts were mixed with 50 g of diacetin. The removal of the extraction solvent from the crude extract samples obtained was carried out on a rotary evaporator at a temperature of 50 ° C and a vacuum of 250 to 40 mbar. The resulting vanilla extracts were each sensed in milk as a matrix in a concentration of 0.1 to 0.3%.
  • the descriptive terms are first collected in the panel, whereby the term lists are structured, similar terms are summarized and hedonic attributes are eliminated.
  • the evaluation of the intensity of the descriptors on a scale of 1 to 10 is carried out by at least ten trained assessors.
  • the samples are coded, tasted in a randomized sequence and to the exclusion of disturbing influences such as color, noise and foreign odors in a sensor room.
  • the final result is determined by summing the individual results and then forming the arithmetic mean and is displayed graphically in the form of a network diagram.
  • the flavoring agents of an acetone-vanilla extract include: phenols: especially guaiacol, 4-methylgujacol, p-cresol, 4-vinylguajacol, 2-vinylphenol, acetovanillon, vanillyl alcohol, p-hydroxybenzaldehyde and p-hydroxybenzyl alcohol, and esters: especially methyl salicylate , Methyl cinnamate and ethyl linolenate.
  • the extracts were prepared as follows:
  • Acetone Extract and Methanol Extract Extraction Temperature: 60 ° C; Pressure: 5 bar: In each case a 400 g crushed vanilla pods were placed in a 2,000 ml pressure vessel (autoclave station F. Strüver) and 800 g of acetone or methanol were added as extraction solvent. Subsequently, a pressure of 2 bar was applied. After heating to 60 ° C, the pressure was adjusted to 5 bar and extracted for 2 h. After cooling, the mixture was passed through a colander and filtered. The comminuted vanilla pods were then extracted twice each consecutively with 800 g acetone or methanol under the same conditions. Before combining the respective crude extracts, a sample was taken from each phase for the analysis. The combined crude extracts were concentrated on a rotary evaporator under a vacuum of 40 mbar, a temperature of 45 ° C and 30 min at final vacuum.
  • Acetone Extract and Methanol Extract Extraction Temperature: 40 ° C; Pressure: Atmospheric pressure: 400 g of shredded vanilla pods were placed in each of a 4,000 ml three-necked flask and 1,000 g of acetone or methanol were added as the extraction solvent. After heating to 40 ° C was extracted for 2 h. After cooling, the mixture was passed through a colander and filtered. The comminuted vanilla preserves were then each extracted twice consecutively with in each case 1000 g of acetone or methanol under the same conditions. Before combining the respective crude extracts, a sample was taken from each phase for the analysis. The combined crude extracts were concentrated on a rotary evaporator under a vacuum of 40 mbar, a temperature of 45 ° C and 30 min at final vacuum.
  • the extracts were prepared as follows: In a 300 ml Erlenmeyer flask with magnetic stirrer were each 37.5 g crushed vanilla pods with 150 g of methanol as extraction solvent at a temperature of 30 ° C, 40 ° C or 65 ° C or 150 g of methanol / water extraction solvent at a temperature of 65 ° C for 2 h. Extracted. The comminuted vanilla pods were then each extracted twice consecutively with 150 g of methanol or 150 g of methanol / water methanol under the same conditions. The combined crude extracts were filtered off. Before the distillation, the respective crude extracts were mixed with 50 g of diacetin.
  • the removal of the extraction solvent from the crude extract samples obtained was carried out on a rotary evaporator at a temperature of 50 ° C and a vacuum of 50 mbar.
  • the vanilla extracts obtained were each judged sensory in milk as a matrix in a concentration of 0.1 to 0.3%.
  • FIG. 4 The influence of the consecutive extraction with acetone or methanol on the vanillin yield is shown in FIG. As can be seen from FIG. 4, significant yields of vanillin are still obtained in extraction stages 2 and 3: the solid line describes the vanillin content of an extraction with acetone: 5,208 mg / kg (extraction 1) plus 1,399 mg / kg (extraction 2) plus 386 mg / kg (extraction 3); the dashed line describes the vanillin content of an extraction with methanol: 5,353 mg / kg (extraction 1) plus 1,620 mg / kg (extraction 2) plus 545 mg / kg (extraction 3). In an exhaustive extraction at a temperature of 40 ° C In addition, the yield of dry matter soluble in acetone or methanol is about 10 to 40%.
  • Acetone Extract and Methanol Extract Extraction Temperature: 40 ° C; Pressure: Atmospheric pressure: 400 g of shredded vanilla pods were placed in each of a 4,000 ml three-necked flask and 1,000 g of acetone or methanol were added as the extraction solvent. After heating to 40 ° C was extracted for 2 h. After cooling, the mixture was passed through a colander and filtered. The comminuted vanilla preserves were then each extracted twice consecutively with in each case 1000 g of acetone or methanol under the same conditions. Before combining the respective crude extracts, a sample was taken from each phase for the analysis. The combined crude extracts were concentrated on a rotary evaporator under a vacuum of 40 mbar, a temperature of 45 ° C and 30 min at final vacuum.
  • FIG. 5 The influence of the consecutive extraction with methanol on the vanillin yield at different temperatures is shown in FIG.
  • the solid line describes the vanillin content of an extraction Methanol at 65 ° C: 7,251 mg / kg (Extraction 1) plus 2,031 mg / kg (Extraction 2) plus 616 mg / kg (Extraction 3);
  • the dashed line describes the vanillin content of an extraction with methanol at 25 ° C: 6195 mg / kg (Extraction 1) plus 1446 mg / kg (Extraction 2) plus 305 mg / kg (Extraction 3).
  • the higher solubility and thus the yield of flavorings is limited by the sensors (see above).
  • the extracts were prepared as follows: In a 300 ml Erlenmeyer flask with magnetic stirrer were each 37.5 g crushed vanilla pods with 150 g of methanol as extraction solvent at a temperature of 25 ° C or 65 ° C for 2 h extracted. The comminuted vanilla pods were then each extracted twice consecutively with 150 g of methanol under the same conditions. The combined crude extracts were filtered off. Before the distillation, the respective crude extracts were mixed with 50 g of diacetin. The removal of the extraction solvent from the crude extract samples obtained was carried out on a rotary evaporator at a temperature of 50 ° C and a vacuum of 50 mbar.
  • the present invention further relates to an ethanol-free vanilla extract obtainable by the method described above.
  • the vanilla extract produced by the method according to the invention is characterized in that it contains only residual amounts of ethanol, which occurs naturally in fermented vanilla pods or develops during the course of ripening or fermentation of the vanilla pods, so that it can also be found in such countries can be sold in which for cultural, religious or ideological reasons, the consumption of alcohol is prohibited.
  • vanilla extract produced by the process according to the invention is characterized in that it has an authentic sensor profile with phenolic, balsamic notes that the sensor profile of fermented vanilla bean is closer than a vanilla extract obtained by the same process using ethanol.
  • vanilla extract produced according to the invention is microbiologically stable and does not change the odor type during storage.
  • the acetone-vanilla extract was prepared as described in Example 1 below.
  • the ethanol-vanilla extract was prepared by the same extraction method and from the same batch of fermented vanilla pods as described below in Example 1, except that ethanol was used as the extraction solvent instead of acetone.
  • the volatile compounds are applied to the chromatographic system (capillary column with WAX coating 30 m c 0.25 mm c 0.25 pm), thereby separated (temperature program from 40 ° C at 3 ° C / min to 230 ° C) and mass spectrometry analyzed.
  • the peak areas are then set in relation to 2-nonanol and output as salary data taking into account response factors.
  • the content of the individual compounds of the ethanol and acetone vanilla extracts is given as the area percent based on the peak area of the 2-nonanol used.
  • nn not proven Ethanol forms rum-like flavors in contact with vanilla ingredients, recognizable inter alia in the formation of ethers, esters and acetals, as shown in Table 2. Such compounds are present in the acetone-vanilla extract not at all or only in a small proportion.
  • acetone in contact with vanilla ingredients does not form detectable reaction products.
  • the acetone-based vanilla extract has phenolic, balsamic notes which include, but are not limited to: in the presence of guaiacol, 4-methylgujacol, p-cresol, 4-vinylguajacol, 2-vinylphenol, acetovanillon, vanillyl alcohol, p-hydroxybenzaldehyde and p-hydroxybenzyl alcohol.
  • the present invention thus also relates to an ethanol-free vanilla extract having an ethanol content of at most 100 mg / kg, in particular at most 50 mg / kg.
  • the ethanol content is derived from ethanol, which naturally occurs in the vanilla pods or during the maturation of the vanilla pods, for example as a degradation product of naturally occurring in vanilla pods mono-, di- or polysaccharides arises.
  • the vanilla extract is therefore preferably free of externally added ethanol or free of non-natural ethanol.
  • the vanilla extract is free from ethanolic extraction solvents.
  • the present invention relates to an ethanol-free vanilla extract having a lower content of ether compounds compared to an ethanol vanilla extract having the same concentration, which was prepared by the same extraction method and from the same dried vanilla pods.
  • the composition of the acetone-based vanilla extract has a vanillin ethyl ether content (expressed as area percent) which is lower by at least 70%, preferably by at least 80%, based on the vanillin ethyl ether content ( expressed as area percent) of an ethanol-vanilla extract having the same concentration after that Extraction method and from the same fermented vanilla pods was prepared.
  • the present invention relates to an ethanol-free vanilla extract, the composition of which comprises guaiacol in an amount (expressed as area percent) which is at least 200% greater, based on the amount of guaiacol (expressed as area percent) in one Ethanol-vanilla extract of the same concentration produced by the same extraction method and from the same fermented vanilla pods.
  • the ethanol-free vanilla extract of the present invention is characterized by comprising p-flydroxybenzaldehyde in an amount (expressed as area percent) that is at least 300% larger, based on the amount of p-flydroxybenzaldehyde (expressed as area percent) in an ethanol-vanilla extract of the same concentration produced by the same extraction method and from the same fermented vanilla pods.
  • the present invention relates to an ethanol-free vanilla extract in which the ratio of the guaiacol contained in the vanilla extract to the vanillin contained in the vanilla extract (determined from the peak areas determined by the foregoing chromatographic methodology - GCMS) internal standard 2-nonanol) is in a range of 1 to 0.001.
  • Particularly preferred in this context is a ratio of guaiacol to vanillin in the range of 0.40 to 0.05; most preferred is a ratio in the range of 0.35 to 0.10.
  • the ethanol-free vanilla extract obtained by the method according to the invention, a high ratio of p-flydroxybenzaldehyde with respect to the vanillin contained in the extract.
  • a quantitative ratio of p-flydroxybenzaldehyde to vanillin in the range of 0.001 to 0.1; most preferred is a ratio in the range of 0.02 to 0.08.
  • the ethanol-free vanilla extract obtained by the method according to the invention has a low ratio of acetals, such as, for example, 3-methylbutanal-propylene glycol acetal isomer 1 or 3-methylbutanal propylene glycol acetal isomer 2, and ethers, such as anisethyl ether or Vanillin ethyl ether, relative to the vanillin contained in the ethanol-free vanilla extract.
  • acetals such as, for example, 3-methylbutanal-propylene glycol acetal isomer 1 or 3-methylbutanal propylene glycol acetal isomer 2
  • ethers such as anisethyl ether or Vanillin ethyl ether
  • a particularly preferred embodiment of the present invention relates to an ethanol-free vanilla extract having a ratio of acetal compounds, such as 3-methylbutanal-propylene glycol acetal isomer 1 or 3-methylbutanal-propylene glycol acetal isomer 2, to vanillin in a range of 0, 0005 to 0.01. Most preferred is a ratio of said acetal compounds to vanillin in a range of from 0.0009 to 0.005.
  • the amount ratio of the ether compounds contained in the ethanol-free vanilla extract such as, for example, anisethyl ether or vanillin ethyl ether, to the vanillin contained in a range of 0.001 to 0.1. Most preferred is a ratio of said ether compounds to vanillin in a range of 0.01 to 0.4.
  • vanilla extracts prepared by the process according to the invention
  • a content of guaiacol, p-hydroxybenzaldehyde, ethers and acetals in the areas described above are characterized in that they have a particularly authentic sensor profile with phenolic, balsamic
  • They have notes that closer to the sensorial profile of fermented vanilla pods as comparable vanilla extracts with different ratios of guaiacol, ethers and acetals.
  • the ethanol-free vanilla extract according to the present invention is also characterized by being free of waxes and fats, in particular fatty acids, their glycerides and fatty alkyl esters, which would otherwise cause solubility problems and precipitate ,
  • the ethanol-free vanilla extract according to the invention can be used for the production of foods, luxury foods, beverage products, semi-finished products, hygiene products, cosmetic or pharmaceutical products, tobacco products and products for animal nutrition.
  • the vanilla extract according to the invention can be used for flavoring or reconstituting the aroma of foods, luxury foods, beverage products, semi-finished products, hygiene products, cosmetic or pharmaceutical products, tobacco products and products for animal nutrition.
  • Another aspect of the present invention is therefore also food, semi-luxury foods, beverage products, semi-finished products, hygiene products, cosmetic or pharmaceutical products, tobacco products and animal nutrition products comprising the ethanol-free vanilla extract.
  • the foods to which the vanilla extract of the present invention is added are preferably selected from the group consisting of drinks, milk products, sweets, dietary supplements, dietetic foods and food substitutes, without being limited thereto.
  • 25 kg of cut fermented vanilla pods (Vanilla planifolia) are filled in a Willmespresse and added to the first subset 60 kg of acetone at 40 ° C.
  • the pods are extracted at 40 ° C for 4 hours while rotating the drum, then cooled to 30 ° C and the acetone crude extract is drained through a 200pm filter bag.
  • the pods are extracted a total of four times comparable to the corresponding subset of 60 kg of acetone.
  • the combined crude acetone extracts are mixed with 7.5 kg of 1,2-propanediol and homogenized with stirring.
  • the mixture is filtered through a plate filter with 2.9 mm thick cellulose plates with a water permeability of 10200 L / m 2 / min and exclusion of 700 g / m 2 and placed in a suitable distillation bubble.
  • the crude extract is first freed from> 99% of the acetone. Thereafter, the vacuum is lowered to 20 mbar to remove the water and the last amounts of acetone to less than 1,000 mg / kg.
  • the acetone-depleted vanilla extract is allowed to stand.
  • the vanilla extract separates into a lower liquid polypropylene glycol phase and an upper wax phase.
  • the liquid polypropylene glycol phase (7.5 kg) is separated from the wax phase and the wax phase is discarded.
  • the acetone-based vanilla extract according to the invention prepared by the method described above was analyzed by gas chromatography as follows: 100 mg of extract are placed in a 10 ml glass jar and mixed with 4 g of water with the addition of the internal standard 2-nonanol (20 mg / kg ) diluted. A magnetic stirring bar coated with PDMS (10 mm long, 1 mm layer thickness) is added and stirred for 1 h. The magnetic stir bar is then removed, another magnetic stir bar is used for extraction in the same manner, and both magnetic stir bars are subsequently baked at 150 ° C with a thermal desorption unit on the GC.
  • the volatile compounds are thereby brought to the chromatographic system (capillary column with WAX coating 30 m c 0.25 mm c 0.25 pm), thereby separated (temperature program from 40 ° C at 3 ° C / min to 230 ° C) and mass spectrometry analyzed.
  • the peak areas are then set in relation to 2-nonanol and output as salary data taking into account response factors.
  • composition of the acetone-based vanilla extract is shown in Table 3.
  • Table 3 Composition of volatile components of an acetone-based vanilla extract according to the invention

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Abstract

La présente invention concerne un procédé de fabrication d'un extrait de vanille sans éthanol et un extrait de vanille sans éthanol, qui peut être obtenu selon le procédé selon l'invention. En particulier, la présente invention concerne un extrait de vanille sans éthanol comprenant au plus 100 mg/kg d'éthanol, une utilisation dudit extrait de vanille sans éthanol ainsi que des produits comprenant l'extrait de vanille sans éthanol. La présente invention vise en particulier à fournir un extrait de vanille sans éthanol, qui ne contient que des traces d'éthanol naturellement contenues dans des gousses de vanille fermentées et qui est produit sans l'aide de l'éthanol.
EP18712136.3A 2018-03-13 2018-03-13 Production d'extraits de vanille sans éthanol Pending EP3764813A1 (fr)

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CN113662234B (zh) * 2021-08-06 2022-10-04 浙江中创雾联生物科技有限公司 抑菌抗菌的雾化液及其制备方法
FR3134818A1 (fr) * 2022-04-20 2023-10-27 L'oreal Concrete et absolue de parfum obtenues par extraction de solvant acetate de (cyclo)alkyl a partir de matieres naturelles solides
FR3134820A1 (fr) * 2022-04-20 2023-10-27 L'oreal Concrete et absolue de parfum obtenues par extraction de solvant (poly)ol organique a partir de matieres naturelles solides
FR3134819A1 (fr) * 2022-04-20 2023-10-27 L'oreal Concrete et absolue de parfum obtenues par extraction de solvant cetonique ou de type dioxolane a partir de matieres naturelles solides
FR3134816A1 (fr) * 2022-04-20 2023-10-27 L'oreal Concrete et absolue de parfum obtenues par extraction de solvant derive de propionate de (cyclo)alkyle a partir de matieres naturelles solides

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US2835591A (en) * 1955-08-23 1958-05-20 Mccormick & Co Inc Method of producing cured vanilla extract from green vanilla beans
DE3137230A1 (de) 1981-09-18 1983-04-21 Haarmann & Reimer Gmbh, 3450 Holzminden Verfahren zur extraktion der aromastoffe der vanillekapsel
JPH04214799A (ja) 1990-11-22 1992-08-05 T Hasegawa Co Ltd 新規なバニラエキスの製法
US20060088627A1 (en) 2004-10-25 2006-04-27 Sensient Flavors Inc. Methods for the production of food grade extracts
US8709502B2 (en) * 2005-09-23 2014-04-29 Chanel Parfums Beaute Extract of Vanilla planifolia
WO2007034042A2 (fr) 2005-09-23 2007-03-29 Chanel Parfums Beaute Extrait de vanilla planifolia, son procede d’obtention, et composition cosmetique ou dermatologique le contenant
FR3033702B1 (fr) 2015-03-18 2018-04-20 Pierre Fabre Dermo-Cosmetique Procede de preparation d'un extrait de plante avec un compose amphiphile non ionique comme adjuvant d'extraction en milieu aqueux

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