EP3261772A1 - Dispositif de séparation magnétique de particules ferromagnétiques, kit de séparation magnétique des particules, procédé de séparation de particules magnétiques à partir d'une solution, et son utilisation - Google Patents

Dispositif de séparation magnétique de particules ferromagnétiques, kit de séparation magnétique des particules, procédé de séparation de particules magnétiques à partir d'une solution, et son utilisation

Info

Publication number
EP3261772A1
EP3261772A1 EP16713727.2A EP16713727A EP3261772A1 EP 3261772 A1 EP3261772 A1 EP 3261772A1 EP 16713727 A EP16713727 A EP 16713727A EP 3261772 A1 EP3261772 A1 EP 3261772A1
Authority
EP
European Patent Office
Prior art keywords
separation
magnet
magnetic
holder
separation vessel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP16713727.2A
Other languages
German (de)
English (en)
Inventor
Karel KOBERNA
Anna LIGASOVA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Univerzita Palackeho V Olomouci
Original Assignee
Univerzita Palackeho V Olomouci
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univerzita Palackeho V Olomouci filed Critical Univerzita Palackeho V Olomouci
Publication of EP3261772A1 publication Critical patent/EP3261772A1/fr
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Definitions

  • the invention relates to a device and a kit for separation of ferromagnetic particles from a solution, method of separation of magnetic particles and use thereof.
  • Magnetic separations are widely used in a number of areas.
  • One of these areas is also separation using particles, which contain a ferromagnetic component (hereinafter only as ferromagnetic particles), where they are usually associated with other substances, which serve as more or less specific absorbents of the molecules contained in the solution.
  • An example is separation using ferromagnetic particles associated with ExtrAvidin, which serves for the isolation of substances containing biotin.
  • Another example are ferromagnetic particles, which are associated with a protein A or particles used for the isolation of nucleic acids. These particles are then utilizable for the separation of complexes, which are associated with antibodies.
  • separators For magnetic separations, special separators are commonly used. The most frequent method is placement of a vessel with ferromagnetic particles into a device, which is equipped with a vessel holder and a magnet. Considering the level of the pole, the vessels in these separators are tilted in such a way that the separation occurs only in a certain part of the vessel (Fig. 1). The activity of the magnet causes the concentration of the particles on the wall and thus makes possible, e.g. subsequent removal of the solution from the vessel for instance using pipettes, without there being a concurrent intake of ferromagnetic particles with the isolated substance. These separators are most frequently produced for a unique size of the vessel. It is necessary to purchase another separator for a different size.
  • a certain disadvantage is also the circumstance that the ferromagnetic particles are typically attacked along a large part of the wall closely neighbouring the bottom of the vessel or reaching even the bottom itself. It is caused by the fact that the difference in the magnetic force at different distances is not so great that it would suffice for the concentration of the particles in the narrow areas above the bottom. Therefore, relatively careful checking is required in the course of the removal of the solution so that there is not a concurrent removal of a part of the particles. In the case of a low concentration of the separated particles difficulties might arrive in determining whether or not there has been a successful separation due to a uniform distribution over a relatively large area.
  • the present invention describes a device for magnetic separation of ferromagnetic particles.
  • the described device enables to control the position of separated ferromagnetic particles in a separation vessel and to achieve a situation when the separated particles are located in a very narrow area of the wall of the separation vessel.
  • the device further enables to use separation vessels of various sizes. The following observations were used during a construction of the device:
  • the ferromagnetic particles are freely distributed in the solution in a fully filled separation vessel, which has a form of substantially a cylinder, and if we attach this cylinder close to a wall of a magnet in such a way that the lengthwise axis of the separation vessel is oriented in parallel with the wall of the magnet connecting its south and north magnetic poles, wherein the separation vessel is longer on both sides than the length of the magnet, the particles concentrate in the area of the poles of the magnet (Fig. 2).
  • this separation vessel is shifted so that its bottom is above the level of an edge of one of the magnetic poles of the magnet, and/or the separation vessel used has a shape, which ensures that its bottom is in a greater distance from the magnetic pole of the magnet than any other part of the separation vessel from the opposite magnetic pole (Fig. 3 and 4), then the highest concentration of the ferromagnetic particles is located in the area of the edge of the opposite magnetic pole of the magnet.
  • the device according to the present invention contains a magnet and a separation vessel holder, ensuring the position of separation vessels towards the magnet. According to our observations, the optimal separation is achieved when the magnet is placed in such a way that areas of the wall of the separation vessel, where the separation of ferromagnetic particles is required by the user, are as close as possible to an edge of a north or a south magnetic pole of the magnet.
  • the magnet touches the wall of the separation vessel by its edge, whereas the distance between the edge of the magnet and the wall of the separation vessel must always be shorter than the distance of any other part of the vessel from the edge of the opposite magnetic pole (Figs. 3 to 7).
  • This arrangement is possible to achieve by fixing of separation vessels and/or by the position of the magnet and/or by the shape of the separation vessels.
  • the vessels are fixed in a way that the bottom of the separation vessel is vertically above the area of the magnetic pole, which is opposite to the magnetic pole whose edge is used for separation (Fig.
  • the present invention describes a device for magnetic separation of ferromagnetic particles from solutions, the said device containing a magnet, preferably of a shape of a right prism, more preferably a right rectangular prism, and a separation vessel holder, wherein the separation vessel holder is placed in such a way towards the magnet that each separation vessel would, upon its placement in the separation vessel holder, be located by the area of its wall, whereon the separation is to take place, close to an edge of the north or the south pole of the magnet, whereas the distance of any other part of the separation vessel from an edge of the opposite pole of the magnet is always greater than the distance between the areas of the wall, whereon the separation is to take place, and the edge of the pole of the magnet serving for the separation.
  • the device contains a holder for the magnet.
  • the device according to the invention further contains a board, whereon the holder of the magnet is optionally placed, and under which a separation plate is located, preferably exchangeably, wherein the board contains a recess for the holder of the magnet or for the magnet, and openings for pins serving to mount the separation vessel holder, preferably exchangeable, with openings for separation vessels.
  • the openings for separation vessels are located in opposite plates, whereas the magnet is placed in between these plates.
  • the separation vessel holder is hence formed by a pair of mutually parallel plates, which contain openings for various sizes of separation vessels, whereas the magnet is placed in between these two plates, preferably within a piece connecting the two parallel plates, in such a way to enable the use of various sizes of separation vessels, according to the needs of the user.
  • the separation vessel holder is exchangeable.
  • the device according to the present invention is made, with the exception of the magnet and optional ferromagnetic inserts for holding the magnet and screws, from a material, which is not attracted by the magnetic field of the magnet, preferably selected from a group comprising thermoplastics, stainless non-magnetic steel and alloys of aluminium.
  • the holder of the magnet is made from ferromagnetic material.
  • the magnet is a permanent magnet, preferably selected from a group comprising a neodymium magnet, samarium-cobalt magnet or an AINiCo magnet.
  • the device according to the invention contains pins, attached to the board fixing the magnet or the holder of the magnet, and exchangeable separation vessel holders in the form of small plates slid on the pins, which fit in the openings in these small plates.
  • the subject of the present invention is also a kit for magnetic separation, containing the device according to the invention, and at least one separation vessel, wherein the magnet has the shape of a right prism, preferably a right rectangular prism, and the separation vessels have a conical shape or a shape of substantially a cylinder with a flat, conical or rounded bottom, wherein the magnet is placed in such a way that its axes, connecting its north pole and its south pole, form together with the axes of the separation vessels, connecting the bottom and the neck of the separation vessel, an angle equal to or greater than 0° and less than 90 ° , preferably from 0° to 80°.
  • the kit for magnetic separation has a magnet in the shape of a right prism, preferably of a right rectangular prism, and the separation vessels have a conical shape and/or a shape of substantially a cylinder with a flat, conical or rounded bottom, wherein the magnet is placed in such a way that its axes, connecting its north pole with its south pole, form together with the axes of the separation vessels, connecting the bottom and the neck of the separation vessel, an angle of 0°.
  • the separation vessels are made from a material, which is not attracted by the magnetic field of the magnet, preferably selected from the group comprising thermoplastics.
  • the subject of the present invention is also a method of separation of magnetic particles from a solution, wherein the solution is transferred into a separation vessel, and subsequently the separation vessel is placed in the proximity of the magnet of the shape of a right prism, preferably of a right rectangular prism, in such a way that the separation vessel is placed by the area of its wall, whereon the separation is to take place, close to the edge of the north or south pole of the magnet, whereas the distance of any other part of the separation vessel from the edge of the opposite pole of the magnet is always greater than the distance between the area of the wall, whereon the separation takes place, and the edge of the pole of the magnet serving for the separation.
  • the subject of the present invention is also the use of the device or the kit according to the invention for separation of ferromagnetic particles from a solution, mixture or suspension.
  • Fig. 1 Schema of the mutual placement of a magnet j_ and a separation vessel A in a classic separator.
  • the magnetic poles are depicted with different shades.
  • the magnet is placed with the magnetic pole towards the separation vessel containing ferromagnetic particles.
  • the ferromagnetic particles are concentrated in the area Al.
  • Fig. 2 (Disclosure of the Invention): In the depicted orientation of a magnet j_ and a separation vessel A, the greatest accumulation of ferromagnetic particles in the separation vessel takes place in the areas of Al adjacent to the edges of the magnetic poles.
  • Fig. 3 Disclosure of the Invention: In the depicted mutual position of a magnet j_ and a separation vessel A, where the bottom of the separation vessel is placed vertically above the edge of the magnetic pole of the magnet, the greatest accumulation of separated ferromagnetic particles takes place in the area of Al of the upper edge of the magnet. This arrangement allows a precise control of the position of the ferromagnetic particles in the separation vessel. According to our experience, this is a sufficient solution for most of the situations.
  • Fig. 4 Disclosure of the Invention: When using a separation vessel A with a rounded or conical bottom, the sedimentation of the separated ferromagnetic particles is minimized in the area of the bottom, thanks to the distance of the bottom from the magnet J_. The accumulation of the separated ferromagnetic particles takes place in the area Al of the edge of the magnet.
  • Fig. 5 Disclosure of the Invention: In the depicted orientation of a magnet I and a wall of a separation vessel A, the accumulation of ferromagnetic particles occurs almost exclusively in the area Al. This arrangement allows the most precise accumulation of the ferromagnetic particles in the required position defined by the edge of the magnet.
  • Fig. 6 Disclosure of the Invention: When using a separation vessel A with an inclined wall and a magnet I, it possible to use also this arrangement for ensuring a maximal accumulation of ferromagnetic particles in the area Al.
  • Fig. 7 (Disclosure of the Invention): It does not matter at all whether the edge of a north or a south pole of a magnet1 is used for separation in separation vessels A. An accumulation of ferromagnetic particles takes place in the areas Al.
  • FIG. 8 A 3D model of a device according to Example 1 (A), view from the front (B) and from the side (C).
  • the following parts are visible in the images: A board 4 with a recess 4C for a holder 2 of a magnet I, with openings 4B for pins 5 and with openings 4A for screws 7, a separation plate 3 with openings, enabling by its exchange with another separation plate of a different thickness to change the height of the magnet1, exchangeable separation vessel holders 6 equipped with openings 6B for separation vessels and a pair of openings 6A, which are complementary to the mounting means on the pins 5.
  • the holder 2 of the magnet I contains openings 2A with threads for the screws 7.
  • Fig. 9 A 3D model of a device from Example 4 (A) and its side view (B).
  • Fig. 10 A 3D model of a device from Example 6 (A) and its side view (B).
  • Fig. 11 A 3D model of a device from Example 9 (A) and its side view (B).
  • Fig. 12 A 3D model of a device from Example 11 (A) and its side view (B).
  • the body 8 of the device contains openings 6B for separation vessels and at the same time serves as a holder of the magnet1.
  • Fig. 13 A 3D model of a device from Example 14 (A) and its side view (B). Examples
  • Example 1 Device for magnetic separation of ferromagnetic particles
  • FIGs. 8 A through 8C the device for separation of ferromagnetic particles is depicted for 3 various sizes of separation vessels.
  • the device is composed of a board 4 with openings 4B for pins 5, with openings 4A for screws 7 for mounting a holder 2 of a magnet j_ and with a recess 4C for the holder 2 of the magnet I and for a separation plate 3, three types of separation vessel holders 6 with one row of openings 6B for separation vessels and openings 6A for the pins 5, the holder 2 of the magnet I with openings 2A furnished with threads for mounting screws, the magnet I, exchangeable separation plates 3 of various thickness with openings for screws 7 for mounting the holder 2 of the magnet 1, two screws 7 for mounting the holder 2 of the magnet1 and four pins 5, which are mounted in the board 4.
  • the pins 5 are furnished with mounting means, which are complementary to the openings 6A in the separation vessel holders.
  • the placement of the separation vessel holders 6 at suitable heights for various separation vessels is achieved by the use of various sizes of those mounting means and their complementary openings 6A in the separation vessel holders 6.
  • the separation vessels are fixed in the separation vessel holder 6 using mounting means on the separation vessels or using the board 4.
  • the separation plates 3 allow for the control of the height of the placement of the magnet 1 in relation to the separation vessels. Two different separation vessel holders 6 can be used at the same time.
  • the board 4 and the separation vessel holders 6 are made from polycarbonate. Alternatively, Plexiglas, polyethylene, polypropylene or ABS (acrylnitril butadien styrene) were used.
  • the holder 2 of the magnet 1, the separation plates 3 and the pins 5 were fabricated from aluminium or non-magnetic steel. Alternatively, polyvinylidene fluoride, polyethylene terephthalate or polyamide were used. A neodymium magnet I in the shape of a right square prism magnetized in the direction of its width was used. Alternatively, a samarium-cobalt magnet, AINiCo magnet or ferrite magnet were used.
  • the magnet I was attached to the holder 2 of the magnet J_, alternatively the holder 2 of the magnet I contained a recess for a ferromagnetic metal plate, which was glued, moulded or embedded into the body of the holder when placed on the side of the magnet, whereon the magnet was placed with no need of gluing it.
  • the recess can be located on the opposite side of the holder of the magnet than there is the magnet. In the latter case, it was not necessary to glue in the ferromagnetic metal plate. The magnetic force of the magnet was sufficient for its fixation and at the same time for the fixation of the magnet.
  • Magnet j_ was placed in such a way that its axis connecting its magnetic poles forms with the axis connecting the bottom and the neck of the separation vessel an angle of approximately 45 ° . Thanks to the possibility to combine various separation vessel holders on opposite sides of the magnet at the same time, the device allows concurrent separation in at least two types of separation vessels. In the case that the separation vessels have the shape of a cylinder of roughly the same diameter, it is possible to use several types of separation vessels for magnetic separation. Thanks to the use of separation plates 3 of various thickness and various separation vessel holders 6, it is possible to achieve different heights of separation of ferromagnetic particles.
  • Example 2 Device for magnetic separation.
  • the device is identical with the device from Example 1 with the following difference:
  • the shape of the magnet I is a right prism, preferably a right rectangular prism, which is oriented so that its axis connecting its magnetic poles forms with the axis connecting the bottom and neck of the separation vessels an angle greater than 0 ° and less than 90 ° .
  • Example 3 Kit for magnetic separation.
  • the kit for magnetic separation contains the device from Example 1 or 2 and also separation vessels of a conical shape or of a shape of substantially a cylinder with a flat, conical or rounded bottom.
  • the separation vessels were made from thermoplastics. These cylindrical separation vessels have the same diameter, but different lengths and hence also volumes. This makes it possible for a single set of separation vessel holders to be used for separation in several types of separation vessels. Moreover, thanks to the use of the separation plates 3, it is possible to easily control the height at which the separation of ferromagnetic particles takes place in all of these cylindrical types separation vessels.
  • Example 4 Device for magnetic separation (Fig. 9 A and 9B).
  • the device is identical with the device from Example 1 with the following difference:
  • the shape of the magnet j_ is a right prism, preferably a right rectangular prism and the magnet j_ is oriented in a way that its axis connecting its magnetic poles forms with the axis connecting the bottom and neck of the separation vessels an angle of 0° .
  • the holder 2 of the magnet1 is composed of a non-magnetic plate, whereon the magnet is glued, or it is made from magnetic stainless steel. In the latter case, the magnetic force serves for attaching the magnet j_.
  • the holder 2 of the magnet I is attached to the board 4 using screws 7.
  • a plate from ferromagnetic material such as of stainless steel is inserted into the recess for the magnet.
  • this plate is fixed using gluing, moulding, pressing in or using screws, and the magnet is then fixed directly to the plate due to its magnetic force.
  • an independent magnet holder is not used.
  • a recess is placed into the underneath side of the board, into which a plate from ferromagnetic material is placed, for example from stainless ferromagnetic steel, which can alternatively be glued, moulded, pressed in or screwed, preferably it is only inserted and the magnetic force of the magnet is sufficient for its fixation. This also ensures the fixation of the magnet.
  • the device does not contain an independent magnet holder. Instead of that, the magnet is placed directly into the recess in the board. Alternatively, the magnet is placed or pressed into the recess without further fixation or it is fixed using a glue with no need to use other fixation elements.
  • Example 5 Kit for magnetic separation.
  • the kit contains the device from Example 4 and also separation vessels with a flat, conical or rounded bottom.
  • Example 6 Device for magnetic separation (Fig. 10A and 10B).
  • the device is identical with the device from Example 1 with the following difference:
  • the board 4 contains two openings 4B for pins 5.
  • the separation vessel holders 6 contain two rows of openings 6B for the separation vessels.
  • the device usually does not allow the simultaneous separation in two types of separation vessels. The exception are cases when cylindrical vessels of approximately the same diameter are used.
  • Example 7 Kit for magnetic separation.
  • the kit contains the device from Example 6 and also separation conical vessels or separation vessels in the shape of substantially a cylinder with a flat, conical or rounded bottom. Those cylindrical separation vessels have the same diameter, but a different length and hence also different volume. This enables for one set of separation vessel holders to be used for the separation using several types of separation vessels. Moreover, when separation plates 3 are used, it is possible to easily control the height at which the separation of ferromagnetic particles takes place in all of these cylindrical separation vessels.
  • Example 8 Device for magnetic separation.
  • the device is identical with the device from Example 4 and in terms of its arrangement it is similar to the device from Example 6, with the following difference:
  • the board 4 contains two openings 4B for pins.
  • the separation vessel holders 6 contain two rows of openings 6B for the separation vessels.
  • Example 4 Compared to the device from Example 6, the orientation of the magnet is the same as in Example 4.
  • the device usually does not allow the simultaneous separation in two different types of separation vessels.
  • the exception are cases when cylindrical vessels of approximately the same diameter are used.
  • vessels with a flat, conical or rounded bottom are used.
  • Example 9 Device for magnetic separation (Fig. 11A and 11B).
  • the device is identical with the device from Example 1 with the following difference:
  • the board 4 contains two openings 4B for pins 5 and provides, due to its special shape, the support for the magnet I.
  • the magnet I in the shape of a right prism, preferably a right rectangular prism, is glued directly to the board 4 or is attached using a plate from stainless ferromagnetic steel, which is glued, moulded or pressed in, in the position in which the area of its magnetic poles forms, with the area of the separation vessel holders 6 with openings 6B for the separation vessels, an angle that is equal to or greater than 0° and less than 90° .
  • the edge of the magnet I is used for separation.
  • the device usually does not allow simultaneous separation in two different types of separation vessels.
  • Example 10 Kit for magnetic separation.
  • the kit contains the device from Example 9 and also conical separation vessels or separation vessels in the shape of substantially a cylinder with a flat, conical or rounded bottom.
  • the cylindrical separation vessels have the same diameter, but different lengths and therefore also different volumes. This makes it possible to use one set of separation vessel holders for the separation of several types of vessels.
  • Example 11 Device for magnetic separation (Fig. 12A and 12B).
  • the device is composed of a body 8 of the separator, constructed as a combination of the separation vessel holder and the holder of the magnet, which further contains a recess for the magnet 1 and openings 6B for the separation vessels.
  • the body 8 of the separator is made from thermoplastics.
  • the magnet I is identical as in Example 1 and also has the same orientation towards the openings for the separation vessels. The magnet is either only inserted in the recess in the body of the separator or it is glued there.
  • Example 12 Device for magnetic separation.
  • the device is identical with the device described in Example 11 with the following difference:
  • Example 13 Device for magnetic separation (Fig. 13A and 13B).
  • the device is composed of a body 8 of the separator, which contains a recess for the magnet I and openings 6B for the separation vessels and the magnet I.
  • the body 8 of the separator serves as a separation vessel holder and at the same time as the holder of the magnet.
  • the body 8 of the separator is made from thermoplastics; the magnet is the same as in Example 1, differing only in its shape.
  • the magnet j_ in a shape of a right prism, preferably a right rectangular prism, is placed within a piece connecting the two parallel plates with openings for the separation vessels.
  • the magnet is alternatively pressed into the recess without further fixation or it is glued in, and it is placed in such a way that its axis, connecting its magnetic poles, forms with the axis, connecting the bottom and neck of the separation vessels, an angle equal to or greater than 0° and less than 90° .
  • the edge of the magnet is used for the separation.
  • the combination of various mutual distances of the opposite plates with the openings for the separation vessels and the distance of these openings from the separation edges of the magnet makes it possible to use one device for separation in at least two types of separation vessels.
  • Example 14 Kit for magnetic separation.
  • the kit contains the device from Example 13 and also separation conical vessels or vessels in the shape of substantially a cylinder with a flat, conical or rounded bottom with two different diameters and different lengths.
  • the device according to the present invention can, for example, be used in fields where magnetic separations are carried out, such as for separation of proteins using antibodies, when ferromagnetic particles are conjugated with protein A.
  • Another example are separations of substances containing biotin in their molecule. In this case, it is possible to use for the separation particles containing a ferromagnetic component and streptavidin.
  • Another example are the non-specific isolations of nucleic acids.

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif de séparation magnétique de particules ferromagnétiques à partir de solutions, qui contient un aimant (1) et un support de cuve de séparation (6), le support de cuve de séparation étant situé par rapport à l'aimant de telle sorte que chaque cuve de séparation (A), lors de son placement dans le support de cuve de séparation, serait placée par la zone de sa paroi, sur laquelle la séparation doit se produire, près d'un bord du pôle nord ou sud de l'aimant, tandis que la distance de toute autre partie de la cuve de séparation à partir d'un bord du pôle opposé de l'aimant est toujours supérieure à la distance entre les zones de la paroi, sur lesquelles la séparation doit se produire, et le bord du pôle de l'aimant servant à la séparation. La présente invention concerne également un kit de séparation magnétique de particules, contenant le dispositif de séparation magnétique de particules ferromagnétiques à partir de solutions et au moins une cuve de séparation. L'invention concerne également un procédé de séparation de particules magnétiques à partir d'une solution, et l'utilisation du dispositif de séparation de particules ferromagnétiques à partir d'une solution, d'un mélange ou d'une suspension.
EP16713727.2A 2015-02-26 2016-02-26 Dispositif de séparation magnétique de particules ferromagnétiques, kit de séparation magnétique des particules, procédé de séparation de particules magnétiques à partir d'une solution, et son utilisation Ceased EP3261772A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CZ2015-141A CZ2015141A3 (cs) 2015-02-26 2015-02-26 Zařízení pro magnetickou separaci feromagnetických částic, sada pro magnetickou separaci částic, způsob separace magnetických částic z roztoku a použití zařízení nebo sady pro magnetickou separaci částic
PCT/CZ2016/050006 WO2016134683A1 (fr) 2015-02-26 2016-02-26 Dispositif de séparation magnétique de particules ferromagnétiques, kit de séparation magnétique des particules, procédé de séparation de particules magnétiques à partir d'une solution, et son utilisation

Publications (1)

Publication Number Publication Date
EP3261772A1 true EP3261772A1 (fr) 2018-01-03

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EP16713727.2A Ceased EP3261772A1 (fr) 2015-02-26 2016-02-26 Dispositif de séparation magnétique de particules ferromagnétiques, kit de séparation magnétique des particules, procédé de séparation de particules magnétiques à partir d'une solution, et son utilisation

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EP (1) EP3261772A1 (fr)
CZ (1) CZ2015141A3 (fr)
WO (1) WO2016134683A1 (fr)

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DE202021105458U1 (de) * 2021-10-08 2023-01-24 Sanolibio Co., Ltd. Vorrichtung zur magnetischen Aufreinigung biologischer Proben

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