EP2449125A4 - Blocage, extraction et détection combinés d'acide nucléique dans un réceptacle de réaction unique - Google Patents

Blocage, extraction et détection combinés d'acide nucléique dans un réceptacle de réaction unique

Info

Publication number
EP2449125A4
EP2449125A4 EP10794424.1A EP10794424A EP2449125A4 EP 2449125 A4 EP2449125 A4 EP 2449125A4 EP 10794424 A EP10794424 A EP 10794424A EP 2449125 A4 EP2449125 A4 EP 2449125A4
Authority
EP
European Patent Office
Prior art keywords
extraction
detection
nucleic acid
reaction vessel
single reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP10794424.1A
Other languages
German (de)
English (en)
Other versions
EP2449125A2 (fr
Inventor
David James Saul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZyGEM Corp Ltd
Original Assignee
ZyGEM Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ZyGEM Corp Ltd filed Critical ZyGEM Corp Ltd
Publication of EP2449125A2 publication Critical patent/EP2449125A2/fr
Publication of EP2449125A4 publication Critical patent/EP2449125A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP10794424.1A 2009-07-02 2010-07-02 Blocage, extraction et détection combinés d'acide nucléique dans un réceptacle de réaction unique Withdrawn EP2449125A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22291209P 2009-07-02 2009-07-02
PCT/NZ2010/000137 WO2011002319A2 (fr) 2009-07-02 2010-07-02 Blocage, extraction et détection combinés d'acide nucléique dans un réceptacle de réaction unique

Publications (2)

Publication Number Publication Date
EP2449125A2 EP2449125A2 (fr) 2012-05-09
EP2449125A4 true EP2449125A4 (fr) 2013-05-29

Family

ID=43411643

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10794424.1A Withdrawn EP2449125A4 (fr) 2009-07-02 2010-07-02 Blocage, extraction et détection combinés d'acide nucléique dans un réceptacle de réaction unique

Country Status (3)

Country Link
EP (1) EP2449125A4 (fr)
JP (1) JP2012531907A (fr)
WO (1) WO2011002319A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2324128A4 (fr) * 2008-08-14 2012-09-12 Zygem Corp Ltd Procédé de détection d'acide nucléique à température contrôlée adapté pour être mis en pratique dans un système fermé
US9294603B2 (en) 2009-09-16 2016-03-22 Try Safety First, Inc. Standard mobile communication device distraction prevention and safety protocols
PL2478505T3 (pl) * 2009-09-16 2017-09-29 John J. Fischer Standardowe protokoły bezpieczeństwa i zabezpieczenia przed rozproszeniem uwagi przez mobilne urządzenie komunikacyjne
CN104619595B (zh) 2012-07-05 2017-08-22 P.C.O.A.设备有限公司 药物分配器
WO2014025500A2 (fr) * 2012-07-16 2014-02-13 Microlab Horizon Llc Extractions à base d'enzyme thermostable sur une puce microfluidique intégrée pour l'analyse biologique
AU2013298086B2 (en) 2012-07-30 2017-07-20 P.C.O.A. Devices Ltd A receptacle for containing and dispensing solid medicinal pills
IL233295B (en) 2014-06-22 2019-11-28 Ilan Paz A control pill dispensing system
WO2016065300A1 (fr) * 2014-10-24 2016-04-28 Eshoo Mark W Cartouche microfluidique
JP6481364B2 (ja) * 2014-12-26 2019-03-13 東洋紡株式会社 核酸除去法
IL238387B (en) 2015-04-20 2019-01-31 Paz Ilan Drug dispenser release mechanism
CN115350095A (zh) 2015-10-15 2022-11-18 东森塔克斯公司 基于图像识别的剂型分配器
WO2017077529A1 (fr) 2015-11-02 2017-05-11 P.C.O.A. Contenants de distribution de formes pharmaceutiques orales pouvant être avancés et verrouillés
CN107988046B (zh) * 2018-01-23 2021-02-19 吉林大学 基于lamp的自吸式多通道病原菌检测微流控芯片
JP2020162586A (ja) * 2019-03-29 2020-10-08 国立研究開発法人農業・食品産業技術総合研究機構 生物の死亡時期の判定方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010019898A1 (fr) * 2008-08-14 2010-02-18 Zygem Corporation Limited Procédé de détection d'acide nucléique à température contrôlée adapté pour être mis en pratique dans un système fermé

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0572057A1 (fr) * 1992-05-11 1993-12-01 Johnson & Johnson Clinical Diagnostics, Inc. Composition et kit de réactifs de PCR et procédés pour amplification et détection avec amplification non-spécifique d'acides nucléiques réduite
US20080220425A1 (en) * 1996-01-24 2008-09-11 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
NZ511680A (en) * 2001-05-14 2004-07-30 Univ Waikato Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
JP4473878B2 (ja) * 2003-01-29 2010-06-02 ４５４ コーポレーション 核酸を増幅および配列決定する方法
SG178651A1 (en) * 2004-06-17 2012-03-29 Zygem Corp Ltd Purification methods and uses thereof
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
US20080026429A1 (en) * 2006-03-13 2008-01-31 Waikatolink Limited Solution pre-treatment
NZ548731A (en) * 2006-07-24 2008-12-24 Zygem Corp Ltd Isothermal detection methods and uses thereof
JP2008048648A (ja) * 2006-08-23 2008-03-06 Institute Of Physical & Chemical Research 核酸増幅用プライマーセット及び核酸の増幅方法
CN101842159B (zh) * 2007-08-09 2014-09-24 赛路拉公司 关联的多参数单细胞测定及残余生物材料回收的方法和装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010019898A1 (fr) * 2008-08-14 2010-02-18 Zygem Corporation Limited Procédé de détection d'acide nucléique à température contrôlée adapté pour être mis en pratique dans un système fermé

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOSS D ET AL: "An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase", INTERNATIONAL JOURNAL OF LEGAL MEDICINE, SPRINGER VERLAG, DE, vol. 117, no. 6, 1 December 2003 (2003-12-01), pages 340 - 349, XP002476705, ISSN: 0937-9827, [retrieved on 20031023], DOI: 10.1007/S00414-003-0400-9 *

Also Published As

Publication number Publication date
WO2011002319A2 (fr) 2011-01-06
EP2449125A2 (fr) 2012-05-09
WO2011002319A3 (fr) 2011-04-14
JP2012531907A (ja) 2012-12-13

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