EP2449125A4 - Combined nucleic acid blocking, extraction, and detection in a single reaction vessel - Google Patents

Combined nucleic acid blocking, extraction, and detection in a single reaction vessel

Info

Publication number: EP2449125A4
Authority: EP; European Patent Office
Prior art keywords: extraction; detection; nucleic acid; reaction vessel; single reaction
Prior art date: 2009-07-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP10794424.1A

Other languages

German (de)

French (fr)

Other versions

EP2449125A2 (en

Inventor

David James Saul

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

ZyGEM Corp Ltd

Original Assignee

ZyGEM Corp Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2009-07-02

Filing date

2010-07-02

Publication date

2013-05-29

2010-07-02 Application filed by ZyGEM Corp Ltd filed Critical ZyGEM Corp Ltd

2012-05-09 Publication of EP2449125A2 publication Critical patent/EP2449125A2/en

2013-05-29 Publication of EP2449125A4 publication Critical patent/EP2449125A4/en

Status Withdrawn legal-status Critical Current

Links

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

EP10794424.1A 2009-07-02 2010-07-02 Combined nucleic acid blocking, extraction, and detection in a single reaction vessel Withdrawn EP2449125A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US22291209P	2009-07-02	2009-07-02
PCT/NZ2010/000137 WO2011002319A2 (en)	2009-07-02	2010-07-02	Combined nucleic acid blocking, extraction, and detection in a single reaction vessel

Publications (2)

Publication Number	Publication Date
EP2449125A2 EP2449125A2 (en)	2012-05-09
EP2449125A4 true EP2449125A4 (en)	2013-05-29

Family

ID=43411643

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP10794424.1A Withdrawn EP2449125A4 (en)	2009-07-02	2010-07-02	Combined nucleic acid blocking, extraction, and detection in a single reaction vessel

Country Status (3)

Country	Link
EP (1)	EP2449125A4 (en)
JP (1)	JP2012531907A (en)
WO (1)	WO2011002319A2 (en)

Families Citing this family (14)

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Publication number	Priority date	Publication date	Assignee	Title
WO2010019898A1 (en) *	2008-08-14	2010-02-18	Zygem Corporation Limited	Temperature controlled nucleic-acid detection method suitable for practice in a closed-system
AU2010297001A1 (en) *	2009-09-16	2012-03-29	John J. Fischer	Standard mobile communication device distraction prevention and safety protocols
US9294603B2 (en)	2009-09-16	2016-03-22	Try Safety First, Inc.	Standard mobile communication device distraction prevention and safety protocols
WO2014006620A1 (en)	2012-07-05	2014-01-09	P.C.O.A. Devices Ltd.	Medication dispenser
WO2014025500A2 (en) *	2012-07-16	2014-02-13	Microlab Horizon Llc	Thermostable enzyme-based extractions on an integrated microfluidic chip for biological analysis
NO2879974T3 (en)	2012-07-30	2018-01-20
IL233295B (en)	2014-06-22	2019-11-28	Ilan Paz	A controlled pill-dispensing system
WO2016065300A1 (en) *	2014-10-24	2016-04-28	Eshoo Mark W	Microfluidic cartridge
JP6481364B2 (en) *	2014-12-26	2019-03-13	東洋紡株式会社	Nucleic acid removal method
IL238387B (en)	2015-04-20	2019-01-31	Paz Ilan	Medication dispenser depilling mechanism
AU2016337639B2 (en)	2015-10-15	2021-07-01	DosentRX Ltd	Image recognition-based dosage form dispensers
WO2017077529A1 (en)	2015-11-02	2017-05-11	P.C.O.A.	Lockable advanceable oral dosage form dispenser containers
CN107988046B (en) *	2018-01-23	2021-02-19	吉林大学	LAMP-based self-suction type multi-channel pathogen detection micro-fluidic chip
JP2020162586A (en) *	2019-03-29	2020-10-08	国立研究開発法人農業・食品産業技術総合研究機構	Method for determining time of death of life form

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2010019898A1 (en) *	2008-08-14	2010-02-18	Zygem Corporation Limited	Temperature controlled nucleic-acid detection method suitable for practice in a closed-system

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EP0572057A1 (en) *	1992-05-11	1993-12-01	Johnson & Johnson Clinical Diagnostics, Inc.	PCR reagent composition, test kit and methods for amplification and detection with reduced nonspecific amplification of nucleic acids
US20080220425A1 (en) *	1996-01-24	2008-09-11	Third Wave Technologies, Inc.	Methods and Compositions for Detecting Target Sequences
NZ511680A (en) *	2001-05-14	2004-07-30	Univ Waikato	Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
DE602004024034D1 (en) *	2003-01-29	2009-12-24	454 Corp	NUCLEIC ACID AMPLIFICATION BASED ON KINGGEL EMULSION
WO2005122709A2 (en) *	2004-06-17	2005-12-29	Zygem Corporation Limited	Purification methods and uses thereof
GB0414815D0 (en) *	2004-07-02	2004-08-04	Secr Defence	Method for stabilising reagents which are useful for nucleic acid amplification
US20080026429A1 (en) *	2006-03-13	2008-01-31	Waikatolink Limited	Solution pre-treatment
NZ548731A (en) *	2006-07-24	2008-12-24	Zygem Corp Ltd	Isothermal detection methods and uses thereof
JP2008048648A (en) *	2006-08-23	2008-03-06	Institute Of Physical & Chemical Research	Primer set for amplification of nucleic acid and method for amplifying nucleic acid
CN101842159B (en) *	2007-08-09	2014-09-24	赛路拉公司	Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material

2010
- 2010-07-02 WO PCT/NZ2010/000137 patent/WO2011002319A2/en active Application Filing
- 2010-07-02 JP JP2012518502A patent/JP2012531907A/en active Pending
- 2010-07-02 EP EP10794424.1A patent/EP2449125A4/en not_active Withdrawn

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2010019898A1 (en) *	2008-08-14	2010-02-18	Zygem Corporation Limited	Temperature controlled nucleic-acid detection method suitable for practice in a closed-system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOSS D ET AL: "An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase", INTERNATIONAL JOURNAL OF LEGAL MEDICINE, SPRINGER VERLAG, DE, vol. 117, no. 6, 1 December 2003 (2003-12-01), pages 340 - 349, XP002476705, ISSN: 0937-9827, [retrieved on 20031023], DOI: 10.1007/S00414-003-0400-9 *

Also Published As

Publication number	Publication date
EP2449125A2 (en)	2012-05-09
JP2012531907A (en)	2012-12-13
WO2011002319A3 (en)	2011-04-14
WO2011002319A2 (en)	2011-01-06

Legal Events

Date	Code	Title	Description
2012-04-08	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2012-05-09	17P	Request for examination filed	Effective date: 20120201
2012-05-09	AK	Designated contracting states	Kind code of ref document: A2 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
2012-10-10	DAX	Request for extension of the european patent (deleted)
2013-05-29	A4	Supplementary search report drawn up and despatched	Effective date: 20130425
2013-05-29	RIC1	Information provided on ipc code assigned before grant	Ipc: C12P 19/34 20060101AFI20130419BHEP Ipc: C12Q 1/68 20060101ALI20130419BHEP
2014-05-09	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2014-06-11	18D	Application deemed to be withdrawn	Effective date: 20131126

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