EP1476554A1 - Novel compound for inhibiting serine proteases and for inhibiting viral infections or viral propagation: rld 8564 - Google Patents
Novel compound for inhibiting serine proteases and for inhibiting viral infections or viral propagation: rld 8564Info
- Publication number
- EP1476554A1 EP1476554A1 EP03742557A EP03742557A EP1476554A1 EP 1476554 A1 EP1476554 A1 EP 1476554A1 EP 03742557 A EP03742557 A EP 03742557A EP 03742557 A EP03742557 A EP 03742557A EP 1476554 A1 EP1476554 A1 EP 1476554A1
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- EP
- European Patent Office
- Prior art keywords
- peptide
- rld
- fragments
- analogs
- derivatives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8135—Kazal type inhibitors, e.g. pancreatic secretory inhibitor, ovomucoid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Definitions
- the present invention relates to a polypeptide (protein) with serine proteinase-inhibitory, in particular plasmin and elastase-inhibitory properties and inhibitory properties against viruses: RLD 8564 (recombinant 15th LEKTI domain).
- the invention further comprises fragments and / or derivatives derived from the RLD 8564 and the combination of these peptide active ingredients.
- a drug containing the natural, recombinant and synthetic peptides for use in medical indications and for use as a diagnostic agent.
- RLD 8564 the 15th domain of the serine proteinase inhibitor LEKTI, was heterologously expressed and purified in E. coli using conventional RT-PCR and standard cloning methods.
- the recombinant peptide obtained has the following amino acid sequence in the one-letter code:
- RLD 8564 consists of 78 amino acids and has a molecular mass of 8564 Da.
- the six cysteines are preferably bridged according to the Kazal type (1-5, 2-4, 3-6) known for inhibitors.
- Serine proteinase inhibitors have been extensively described in their structure at the protein and DNA sequence level and in their biological function (for a review see: Roberts, RM et al. (1995), Crit. Rev. Eukaryot. Gene Expr., 5 ( 3-4): 385-436). Fragments, analogs and / or derivatives of RLD 8564 are substances with biological properties comparable to those of RLD 8564, which can be structurally derived from the sequence of RLD 8564. Derived structures are, in particular, those that
- N-terminal are shortened by 1, 2 or 3 amino acids
- C-terminal are shortened by 1, 2 or 3 amino acids
- Derived peptides can also exhibit several of these changes.
- Comparable properties mean at least 50% of the inhibition properties on plasmin and elastase according to the test methods described here, ie at most twice the IC 50 value.
- the IC 50 (inhibitory concentration 50%) was 9xl0 "7 M.
- the data generated in this way show (FIGS. 1 and 2) that the peptide RLD 8564, which corresponds to the 15th LEKTI domain, is not only capable of the serine proteinase trypsin (Magert, HJ. Et al, (2002) Int. J. Biochem. Cell Biol, 34 (6), 573-576), but surprisingly also inhibit human plasmin and human leukocyte elastase. RLD 8564 can therefore protect against the proteolytic activity of these enzymes.
- Plasmin is a serine proteinase that plays an important role in inflammatory processes, but especially in fibrinolysis.
- regulation of the proteolytic activity of plasmin by inhibitors is of great physiological importance. This regulation has clinical relevance, for example, in the case of transplants, burns, disorders of the blood coagulation system (fibrinolysis) or liver diseases.
- Elastase has a crucial role in the controlled breakdown of functionally damaged connective tissue (JANOFF, 1985) and thus has important biological functions. Their proteolytic activity must be carefully controlled, as otherwise the enzyme may have pathological attacks on structural proteins in its own organism. An example of a pathological effect that develops due to a disturbance in the balance between proteinase (leukocyte elastase) and its specific inhibitor is pulmonary emphysema. Proteinases can also be released from invaded microorganisms. All these enzymes are able to proteolytically decompose tissues and proteins of the organism and thus also represent an objective danger for the individual concerned. For this reason, a targeted control of the proteinases is necessary.
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to peptide RLD 8564 that has the following amino acid sequence: GPDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEESSTPGTTAASMPPSDE.
Description
VERBINDUNG ZUR INHIBIERUNG DER SERIN-PROTEINASEN UND ZUR INHIBIERUNG VON INFEKTIONEN ODER VIRUSVERMEHRUNG : RLD 8564CONNECTION TO INHIBIT SERIN PROTEINASES AND INHIBIT INFECTION OR VIRUS REPRODUCTION: RLD 8564
Gegenstand der vorliegenden Erfindung ist ein Polypeptid (Eiweißstoff) mit Serin-Proteinase-inhibitorischen, besonders Plasmin- und Elastase- inhibitorischen Eigenschaften sowie inhibitorischen Eigenschaften gegenüber Viren: RLD 8564 (Rekombinante 15. LEKTI-Domäne).The present invention relates to a polypeptide (protein) with serine proteinase-inhibitory, in particular plasmin and elastase-inhibitory properties and inhibitory properties against viruses: RLD 8564 (recombinant 15th LEKTI domain).
Die Erfindung umfasst weiterhin von dem RLD 8564 abgeleitete Fragmente und/oder Derivate sowie die Kombination dieser Peptidwirkstoffe. Schließlich ein Arzneimittel enthaltend die natürlichen, rekombinanten und synthetischen Peptide zur Verwendung für medizinische Indikationen und zur Verwendung als ein Diagnosemittel. Desweiteren eine Nukleinsäuresonde hybridisierend für RLD 8564 oder eines seiner Fragmente und Antikörper bzw. Antagonisten gerichtet gegen RLD 8564 oder eines seiner Fragmente zu diagnostischen oder therapeutischen Zwecken.The invention further comprises fragments and / or derivatives derived from the RLD 8564 and the combination of these peptide active ingredients. Finally, a drug containing the natural, recombinant and synthetic peptides for use in medical indications and for use as a diagnostic agent. Furthermore, a nucleic acid probe hybridizing for RLD 8564 or one of its fragments and antibodies or antagonists directed against RLD 8564 or one of its fragments for diagnostic or therapeutic purposes.
RLD 8564, die 15. Domäne des Serin-Proteinase Inhibitors LEKTI, wurde mittels üblicher RT-PCR und gängiger Klonierungsmethoden heterolog in E.coli exprimiert und aufgereinigt. Das erhaltene rekombinante Peptid besitzt die folgende Aminosäuresequenz im Einbuchstabencode:RLD 8564, the 15th domain of the serine proteinase inhibitor LEKTI, was heterologously expressed and purified in E. coli using conventional RT-PCR and standard cloning methods. The recombinant peptide obtained has the following amino acid sequence in the one-letter code:
GPDSEMCKDYRVLPRIGYLCPKD KPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEESSTP GTTAASMPPSDEGPDSEMCKDYRVLPRIGYLCPKD KPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEESSTP GTTAASMPPSDE
Die ersten zwei Aminosäuren am N-Terminus entsprechen nicht der Sequenz aus dem LEKTI Protein und sind Ursprung der Klonierung und Spaltung des Ausgangspeptides. RLD 8564 besteht aus 78 Aminosäuren und besitzt eine Molekularmasse von 8564 Da. Die sechs Cysteine sind bevorzugt entsprechend dem für Inhibitoren bekannten Kazal-Typ (1-5, 2-4, 3-6) verbrückt. Serin- Proteinase-Inhibitoren wurden in ihrer Struktur auf Protein- und DNA- Sequenzebene und ihrer biologischen Funktion schon umfangreich beschrieben (als Review siehe: Roberts, R.M. et al. (1995), Crit. Rev. Eukaryot. Gene Expr., 5(3-4) :385-436).
Fragmente, Analoga und/oder Derivate des RLD 8564 sind Substanzen mit vergleichbaren biologischen Eigenschaften wie RLD 8564, die sich strukturell von der Sequenz des RLD 8564 ableiten lassen. Abgeleitete Strukturen sind insbesondere solche, dieThe first two amino acids at the N-terminus do not correspond to the sequence from the LEKTI protein and are the origin of the cloning and cleavage of the starting peptide. RLD 8564 consists of 78 amino acids and has a molecular mass of 8564 Da. The six cysteines are preferably bridged according to the Kazal type (1-5, 2-4, 3-6) known for inhibitors. Serine proteinase inhibitors have been extensively described in their structure at the protein and DNA sequence level and in their biological function (for a review see: Roberts, RM et al. (1995), Crit. Rev. Eukaryot. Gene Expr., 5 ( 3-4): 385-436). Fragments, analogs and / or derivatives of RLD 8564 are substances with biological properties comparable to those of RLD 8564, which can be structurally derived from the sequence of RLD 8564. Derived structures are, in particular, those that
• N-terminal um 1, 2 oder 3 Aminosäuren verkürzt sind,N-terminal are shortened by 1, 2 or 3 amino acids,
• C-terminal um 1, 2 oder 3 Aminosäuren verkürzt sind,C-terminal are shortened by 1, 2 or 3 amino acids,
• bei denen maximal 10% der Aminosäuren ausgetauscht oder deletiert sind,In which a maximum of 10% of the amino acids have been exchanged or deleted,
• maximal 5 weitere Aminosäuren eingefügt sind und übliche chemische Derivate wie glykosilierte, amidierte, acetylierte, sulfatierte, phosphorylierte Peptide.• A maximum of 5 additional amino acids and conventional chemical derivatives such as glycosylated, amidated, acetylated, sulfated, phosphorylated peptides are inserted.
Abgeleitete Peptide können auch mehrere dieser Veränderungen aufweisen.Derived peptides can also exhibit several of these changes.
Vergleichbare Eigenschaften bedeutet mindestens 50% der Inhibitionseigenschaften auf Plasmin und Elastase gemäß den hier beschriebenen Testverfahren, d.h. höchstens der doppelte IC50-Wert.Comparable properties mean at least 50% of the inhibition properties on plasmin and elastase according to the test methods described here, ie at most twice the IC 50 value.
Überraschenderweise konnte in einem Inhibitionstest gezeigt werden, dass dieses rekombinant hergestellte Peptid RLD 8564 in der Lage ist, humanes Plasmin (EC-Nr. : 3.4.21.7) und humane Leukozytenelastase (EC-Nr. : 3.4.21.37) zu inhibieren.Surprisingly, it could be shown in an inhibition test that this recombinantly produced peptide RLD 8564 is able to inhibit human plasmin (EC No.: 3.4.21.7) and human leukocyte elastase (EC No.: 3.4.21.37).
Weiterhin konnte in einem Test zur Inhibition der Virusinfektion gezeigt wer- den, dass das rekombinant hergestellte Peptide RLD 8564 in der Lage, bei P4R5 Zellen die HIV-Infektion zu inhibieren (Figur 3).Furthermore, a test to inhibit the viral infection showed that the recombinantly produced peptide RLD 8564 is able to inhibit HIV infection in P4R5 cells (FIG. 3).
Die Daten zeigen, dass das Peptid der 15. LEKTI Domäne (RLD 8564) nicht nur in der Lage ist, Serinproteinasen wie Trypsin zu inhibieren (Magert, HJ. et al (2002), Int. Biochem. Cell Biol., 34(6), 573-576), sondern überraschenderwei- se ebenfalls die HIV-Infektion verringert. Da der HIV-Inhibition eine große Bedeutung zukommt, besitzt dieser neuartige Inhibitor dienische Relevanz bei
der Prophylaxe und Behandlung von AIDS.The data show that the peptide of the 15th LEKTI domain (RLD 8564) is not only able to inhibit serine proteinases such as trypsin (Magert, HJ. Et al (2002), Int. Biochem. Cell Biol., 34 (6 ), 573-576), but surprisingly also reduced the HIV infection. Since HIV inhibition is of great importance, this novel inhibitor has useful relevance the prophylaxis and treatment of AIDS.
Bestimmung der Plasmin-inhibitorischen Aktivität von RLD 8564:Determination of the plasmin-inhibitory activity of RLD 8564:
75 μl 50 mM Tris-HCI, pH 8.0 mit 1 mg/ml BSA und 630 ng/ml human Plasmin (Calbiochem) wurden mit 5 μl Inhibitor (rekombinant hergestelltes Peptid RLD 8564 in unterschiedlichen Konzentrationen) 10 min bei Raumtemperatur inkubiert. Anschließend erfolgte die Zugabe von 20 μl 2,75 mM chromogenen Substrates (N-Tosyl-Gly-Pro-Lys p-Nitroanilide; Sigma T-6140). Die Proteinaseaktivität wurde anhand der Hydrolyse des chromogenen Substrates im Spektralphotometer bei λ=405 nm ermittelt.75 μl 50 mM Tris-HCl, pH 8.0 with 1 mg / ml BSA and 630 ng / ml human plasmin (Calbiochem) were incubated with 5 μl inhibitor (recombinantly produced peptide RLD 8564 in different concentrations) for 10 min at room temperature. Then 20 μl of 2.75 mM chromogenic substrate (N-Tosyl-Gly-Pro-Lys p-nitroanilide; Sigma T-6140) was added. The proteinase activity was determined by hydrolysis of the chromogenic substrate in the spectrophotometer at λ = 405 nm.
Die auf diese Weise erzeugten Daten zeigen (Figur 1), dass das Peptid RLD 8564, welches der 15. LEKTI Domäne entspricht, in der Lage ist Plasmin zu inhibieren.The data generated in this way show (FIG. 1) that the peptide RLD 8564, which corresponds to the 15th LEKTI domain, is able to inhibit plasmin.
Der IC50 (Inhibitory Concentration 50%) betrug 9xl0"7 M.The IC 50 (inhibitory concentration 50%) was 9xl0 "7 M.
Bestimmung der Elastase-inhibitorischen Aktivität von RLD 8564:Determination of the elastase-inhibitory activity of RLD 8564:
85 μl 100 mM Tris-HCI, pH 8.0, lOmM CaCI2 mit 100 μg/ml BSA und 250 ng/ml humaner Leukozytenelastase (Calbiochem, Cat.-Nr. 324681) wurden mit 5 μl Inhibitor (rekombinant hergestelltes Peptid RLD 8564 in unterschiedlichen Konzentrationen) 60 min bei Raumtemperatur inkubiert. Anschließend erfolgte die Zugabe von 10 μl 5 mM chromogenen Substrates (MeOSuc-Ala-Ala-Pro-Val p-Nitroanilide; Calbiochem, Cat.-Nr. 324696). Die Proteinaseaktivität wurde anhand der Hydrolyse des chromogenen Substrates im Spektralphotometer bei λ=405 nm ermittelt. Der IC50-Wert betrug l,lxl0"5 M.85 μl 100 mM Tris-HCl, pH 8.0, 10 mM CaCl 2 with 100 μg / ml BSA and 250 ng / ml human leukocyte elastase (Calbiochem, Cat. No. 324681) were mixed with 5 μl inhibitor (recombinantly produced peptide RLD 8564 in different Concentrations) incubated for 60 min at room temperature. Then 10 μl of 5 mM chromogenic substrate (MeOSuc-Ala-Ala-Pro-Val p-nitroanilide; Calbiochem, Cat. No. 324696) was added. The proteinase activity was determined by hydrolysis of the chromogenic substrate in the spectrophotometer at λ = 405 nm. The IC 50 value was 1.10 × 5 M.
Die auf diese Weise erzeugten Daten zeigen (Figuren 1 und 2), dass das Peptid RLD 8564, welches der 15. LEKTI Domäne entspricht, nicht nur in der Lage ist die Serinproteinase Trypsin (Magert, HJ. et al, (2002) Int. J. Biochem. Cell Biol, 34(6), 573-576), sondern überraschenderweise ebenfalls humanes Plasmin und humane Leukozytenelastase zu inhibieren. RLD 8564 kann somit einen Schutz vor der proteolytischen Aktivität dieser Enzyme darstellen.
Plasmin ist eine Serinproteinase, die eine wichtige Rolle in entzündlichen Prozessen, vor allem aber in der Fibrinolyse spielt. Wie bei allen Proteinasen ist auch hier eine Regulierung der proteolytischen Aktivität von Plasmin durch Inhibitoren von physiologisch größter Wichtigkeit. Klinische Relevanz dieser Regulierung besteht z.B. bei Transplantationen, Verbrennungen, Störungen des Blutgerinnungssystems (Fibrinolyse) oder bei Leberkrankheiten.The data generated in this way show (FIGS. 1 and 2) that the peptide RLD 8564, which corresponds to the 15th LEKTI domain, is not only capable of the serine proteinase trypsin (Magert, HJ. Et al, (2002) Int. J. Biochem. Cell Biol, 34 (6), 573-576), but surprisingly also inhibit human plasmin and human leukocyte elastase. RLD 8564 can therefore protect against the proteolytic activity of these enzymes. Plasmin is a serine proteinase that plays an important role in inflammatory processes, but especially in fibrinolysis. As with all proteinases, regulation of the proteolytic activity of plasmin by inhibitors is of great physiological importance. This regulation has clinical relevance, for example, in the case of transplants, burns, disorders of the blood coagulation system (fibrinolysis) or liver diseases.
Die Elastase besitzt eine entscheidende Aufgabe bei dem kontrollierten Abbau von funktionsgeschädigtem Bindegewebe (JANOFF, 1985) und hat somit wichtige biologische Funktionen. Ihre proteolytische Aktivität muss exakt kontrol- liert werden, da es sonst möglicherweise zu pathologischen Angriffen des Enzyms auf Strukturproteine des eigenen Organismus kommen kann. Ein Beispiel für einen pathologischen Effekt, der sich aufgrund einer Störung im Gleichgewicht zwischen Proteinase (Leukozytenelastase) und ihrem spezifischen Inhibitor entwickelt, ist das Lungenemphysem. Proteinasen können aber auch von eingedrungenen Mikroorganismen freigesetzt werden. Alle diese Enzyme sind in der Lage Gewebe und Proteine des Organismus proteolytisch zu zersetzen und stellen so auch eine objektive Gefahr für das betreffende Individuum dar. Aus diesem Grunde ist eine gezielte Kontrolle der Proteinasen erforderlich.
Elastase has a crucial role in the controlled breakdown of functionally damaged connective tissue (JANOFF, 1985) and thus has important biological functions. Their proteolytic activity must be carefully controlled, as otherwise the enzyme may have pathological attacks on structural proteins in its own organism. An example of a pathological effect that develops due to a disturbance in the balance between proteinase (leukocyte elastase) and its specific inhibitor is pulmonary emphysema. Proteinases can also be released from invaded microorganisms. All these enzymes are able to proteolytically decompose tissues and proteins of the organism and thus also represent an objective danger for the individual concerned. For this reason, a targeted control of the proteinases is necessary.
Claims
1. Das Peptid RLD 8564 mit nachfolgender Aminosäuresequenz:1. The peptide RLD 8564 with the following amino acid sequence:
GPDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEES STPGTTAASMPPSDEGPDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEES STPGTTAASMPPSDE
2. Biologisch aktive und zirkulierende Fragmente, Analoga und/oder Derivate bei Peptide RLD 8564 gemäß Anspruch 1, insbesondere Formen mit dem Austausch einzelner Aminosäuren oder N-terminal oder C-terminal verkürzte oder verlängerte Fragmente sowie glykosylierte, amidierte, acety- lierte, sulfatierte, phosphorylierte sowie die durch multiple Synthese dar- stellbaren Peptide.2. Biologically active and circulating fragments, analogs and / or derivatives in peptides RLD 8564 according to claim 1, in particular forms with the exchange of individual amino acids or N-terminal or C-terminal shortened or extended fragments and glycosylated, amidated, acetylated, sulfated , phosphorylated and the peptides that can be represented by multiple synthesis.
3. Fragment nach Anspruch 2, dadurch gekennzeichnet, dass das Derivat ein N- und/oder C-terminal um eine Aminosäure verkürztes Peptid nach Anspruch 1 ist.3. Fragment according to claim 2, characterized in that the derivative is an N- and / or C-terminal peptide shortened by an amino acid according to claim 1.
4. Fragment nach Anspruch 2 oder 3, dadurch gekennzeichnet, dass das De- rivat ein N- und/oder C-terminal um zwei Aminosäuren verkürztes Peptid nach Anspruch 1 ist.4. Fragment according to claim 2 or 3, characterized in that the derivative is an N- and / or C-terminal peptide shortened by two amino acids according to claim 1.
5. Polynukleotide kodierend für RLD 8564 nach Anspruch 1 und/oder dessen Fragmente, Derivate und Analoga nach mindestens einem der Ansprüche 2 bis 4.5. Polynucleotides coding for RLD 8564 according to claim 1 and / or its fragments, derivatives and analogs according to at least one of claims 2 to 4.
6. Ein Vektor enthaltend das Polynukleotid gemäß Anspruch 5.6. A vector containing the polynucleotide according to claim 5.
7. Eine gentechnisch manipulierte Wirtszelle enthaltend den Vektor nach Anspruch 6.7. A genetically engineered host cell containing the vector of claim 6.
8. Polynukleotid hybridisierend mit dem Polynukleotid gemäß Anspruch 5. 8. Polynucleotide hybridizing with the polynucleotide according to claim 5.
9. Antikörper gerichtet gegen das Peptid und seine Sequenzabschnitte gemäß Anspruch 1.9. Antibodies directed against the peptide and its sequence sections according to claim 1.
10. Antagonist gerichtet gegen das Peptid gemäß Anspruch 1.10. Antagonist directed against the peptide according to claim 1.
11. Arzneimittel enthaltend das Peptid nach Anspruch 1, Fragmente, Analoga und/oder Derivate nach einem der Ansprüche 2 bis 4, der Polynukleotide nach Anspruch 5 oder 8 als wirksamen Bestandteil von galenischen Formen gegebenenfalls in Verbindung mit einem geeigneten Carrier zur oralen, intravenösen, intramuskulären, intracutanen, intrathekalen Anwendung sowie als Aerosol zur transpulmonalen Applikation.11. Medicament containing the peptide according to claim 1, fragments, analogs and / or derivatives according to one of claims 2 to 4, the polynucleotides according to claim 5 or 8 as an effective component of galenic forms, optionally in combination with a suitable carrier for oral, intravenous, intramuscular, intracutaneous, intrathecal use and as an aerosol for transpulmonary application.
12. Verwendung des Peptids nach Anspruch 1, Fragmenten, Analoga und/oder Derivaten nach einem der Ansprüche 2 bis 4, oder der Polynukleotide nach Anspruch 5 oder 8 zur Behandlung von akuten oder chronischen Entzündungen, mit exzessiver Schleimbildung verbundener akut oder chronisch entzündlicher Prozesse und sich daraus ergebener akuter Notsituationen, postoperativen und allgemeinen Blutungen aufgrund Hyperfibrinolyse, zur12. Use of the peptide according to claim 1, fragments, analogs and / or derivatives according to one of claims 2 to 4, or the polynucleotides according to claim 5 or 8 for the treatment of acute or chronic inflammation, acute or chronic inflammatory processes associated with excessive mucus formation and resulting acute emergency situations, postoperative and general bleeding due to hyperfibrinolysis, for
Prophylaxe der Lungenemphysembildung bei z.B. αl-Proteinase- Inhibitormangel, bei akuten und chronischen Haut- und Schleimhautkrankheiten sowie zur Therapie von Asthma und Lungenentzündung zur Behandlung/Prophylaxe von Virusinfektionen, insbesondere HIV-Infektion.Prevention of emphysema formation in e.g. αl-proteinase inhibitor deficiency, in acute and chronic skin and mucous membrane diseases as well as for the therapy of asthma and pneumonia for the treatment / prophylaxis of viral infections, in particular HIV infection.
13. Verwendung des Peptides nach Anspruch 1, Fragmenten, Analoga und/oder Derivaten nach einem der Ansprüche 2 bis 4, des Polynukleotids nach Anspruch 5 oder 8 zur kosmetischen Behandlung von Haut und Schleimhaut.13. Use of the peptide according to claim 1, fragments, analogs and / or derivatives according to one of claims 2 to 4, of the polynucleotide according to claim 5 or 8 for the cosmetic treatment of skin and mucous membrane.
14. Kosmetische Zubereitung, insbesondere Haarpflege oder Reinigungsmittel enthaltend das Peptid nach Anspruch 1, Fragmente, Analoga und/oder De- rivate nach einem der Ansprüche 2 bis 4, oder Polynukleotide nach Anspruch 5 oder 8.14. Cosmetic preparation, in particular hair care or cleaning agent, containing the peptide according to claim 1, fragments, analogs and / or derivatives according to one of claims 2 to 4, or polynucleotides according to claim 5 or 8.
15. Verfahren zur Herstellung von RLD 8564 gemäß Anspruch 1 oder Fragmenten, Analoga und/oder Derivaten nach einem der Ansprüche 2 bis 4 durch15. A process for the preparation of RLD 8564 according to claim 1 or fragments, analogs and / or derivatives according to any one of claims 2 to 4 by
• Festphasensynthese im Sinne der Merrifield-Synthese sowie Flüssigpha- sensynthese nach dem Fachmann bekannten Methoden mit geschützten Aminosäuren und deren Aufreinigung• Solid phase synthesis in the sense of Merrifield synthesis and liquid phase synthesis according to methods known to the person skilled in the art with protected amino acids and their purification
oderor
• durch heterologen Expression mittels gängiger biotechnologischer Vektoren. • by heterologous expression using common biotechnological vectors.
Priority Applications (1)
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EP03742557A EP1476554A1 (en) | 2002-02-22 | 2003-02-20 | Novel compound for inhibiting serine proteases and for inhibiting viral infections or viral propagation: rld 8564 |
Applications Claiming Priority (12)
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DE10207602 | 2002-02-22 | ||
DE10207602 | 2002-02-22 | ||
DE10208302 | 2002-02-26 | ||
DE10208302 | 2002-02-26 | ||
DE10209307 | 2002-03-02 | ||
DE10209307 | 2002-03-02 | ||
DE10220802 | 2002-05-10 | ||
DE10220802 | 2002-05-10 | ||
EP02015418 | 2002-07-11 | ||
EP02015418 | 2002-07-11 | ||
EP03742557A EP1476554A1 (en) | 2002-02-22 | 2003-02-20 | Novel compound for inhibiting serine proteases and for inhibiting viral infections or viral propagation: rld 8564 |
PCT/EP2003/001704 WO2003070953A1 (en) | 2002-02-22 | 2003-02-20 | Novel compound for inhibiting serine proteases and for inhibiting viral infections or viral propagation: rld 8564 |
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DE102009010942A1 (en) | 2009-02-27 | 2010-09-02 | Universitätsklinikum Schleswig-Holstein | Serine protease inhibitors for specific inhibition of tissue kallikreins |
WO2021198176A1 (en) | 2020-03-31 | 2021-10-07 | Pharis Biotec Gmbh | Polypeptide for the prophylaxis and treatment of viral infections |
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CA2375475C (en) * | 1999-06-22 | 2014-10-14 | Forssmann, Wolf-Georg | Serine proteinase inhibitors |
US20030190637A1 (en) * | 2000-03-02 | 2003-10-09 | Alain Hovnanian | Mutations in spink5 responsible for netherton's syndrome and atopic diseases |
WO2002066513A2 (en) * | 2001-02-19 | 2002-08-29 | Ipf Pharmaceuticals Gmbh | Human circulating lekti fragments hf7072, hf7638 and hf14448 and use thereof |
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2003
- 2003-02-20 EP EP03742557A patent/EP1476554A1/en not_active Withdrawn
- 2003-02-20 WO PCT/EP2003/001704 patent/WO2003070953A1/en not_active Application Discontinuation
- 2003-02-20 AU AU2003210317A patent/AU2003210317A1/en not_active Abandoned
Non-Patent Citations (1)
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WO2003070953A1 (en) | 2003-08-28 |
AU2003210317A1 (en) | 2003-09-09 |
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