EP1440732A1 - Configuration microfluidique pour le dosage des liquides - Google Patents

Configuration microfluidique pour le dosage des liquides Download PDF

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Publication number
EP1440732A1
EP1440732A1 EP04001179A EP04001179A EP1440732A1 EP 1440732 A1 EP1440732 A1 EP 1440732A1 EP 04001179 A EP04001179 A EP 04001179A EP 04001179 A EP04001179 A EP 04001179A EP 1440732 A1 EP1440732 A1 EP 1440732A1
Authority
EP
European Patent Office
Prior art keywords
channel
liquid
channels
arrangement according
outlet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP04001179A
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German (de)
English (en)
Other versions
EP1440732B1 (fr
Inventor
Ralf Dr.Dipl.-Phys. Peters
Dirk Osterloh
Gert Dr. Blankenstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Microparts GmbH
Original Assignee
Steag Microparts GmbH
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Publication date
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Publication of EP1440732A1 publication Critical patent/EP1440732A1/fr
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Publication of EP1440732B1 publication Critical patent/EP1440732B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
    • G01F11/28—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with stationary measuring chambers having constant volume during measurement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the present invention relates to a microfluidic arrangement for Dosing and separating one or more first dosed Liquid quantities of a second amount of liquid.
  • microfluidic arrangements are used, for example, for dosing and injecting Liquids for wet-chemical, biochemical and diagnostic Used analyzes.
  • This defined Amount of fluid is excess of a second Liquid quantity separated. Separating the excess Amount of liquid from the metered amount of liquid happens These days usually by mechanical elements or through a gas phase. The gas phase can by suction the excess amount of liquid can be obtained. Likewise is It is possible that the excess amount of liquid by means of a Pressure surge "blown away" by the metered amount of fluid becomes.
  • a disadvantage of the previous microfluidic arrangements for Dosing and separating by means of a gas phase is that for generating the negative pressure or the overpressure (suck off or blow away) for separating the first from the second amount of liquid Pressure generating means must be provided by means of which the necessary pressure surge is generated.
  • On the other hand is for dosing even a pressure surge is not required because the metered Liquid volume often via capillary forces in the dosing is filled. This is e.g. in the publication WO 99/46 045 disclosed.
  • the dosing could in principle with Pressure generating means done by suction or blowing. However, this possibility also proved in the past already as elaborate and, where possible, by filling replaced by capillary forces.
  • the invention is against the background of the disadvantages the prior art arrangements are based on the object, to propose a microfluidic arrangement in which the Separating one or more metered first quantities of liquid from the generally excess second amount of liquid without Pressure generating means is achieved.
  • an inventive Microfluidic arrangement a first channel and one or several second channels.
  • the first channel has an inlet and an outlet. In the area of the outlet is a larger Capillary force before, as in the area of the inlet.
  • the second channels branch at one or more branch points of the first channel off.
  • the second channels have a larger one Capillary force as the first channel at the branch points. Further the second channels have a predetermined volume.
  • a channel in the Essence of the invention may basically be any structure which suitable is a liquid or a gas when transported in one to guide certain direction.
  • the dosing and separating takes place such that one on the Inlet applied liquid quantity due to the capillary force, which is present at the inlet, sucked into the first channel and in Direction of the outlet is transported.
  • the second Channel is filled with the liquid until the defined Volume of the second channel is completely filled.
  • the then still in the first channel contained or still at the inlet fitting Amount of fluid is then due to the capillary force within the first channel transported to its outlet. Is the whole Excess liquid transported to the outlet, lies in the first channel a gas phase before. About this gas phase is the metered amount of liquid in the second channel of the excess amount of fluid fluidly separated.
  • the higher capillary force of the second channel in relation to the first channel at the branching point can be achieved in that at the transition from the first channel to the second channel there is a sudden change in the geometric properties or a sudden change in the surface properties of the wall at the transition.
  • a measure of the capillary force at the transition is the pressure difference, which can be calculated according to the following formula, as also described, for example, in a publication by Hosokawa et. al. Hosokawa, T.Fujii, and I. Endo, "Hydrophobic Microcapillary vent for pneumatic manipulation of liquid in ⁇ Tas", Proc. "Micro Total Analysis Systems '98", pp.
  • ⁇ P -2 ⁇ cos (1 / w + 1 / h - 1 / W - 1 / H), where ⁇ and ⁇ denote the surface tension of the liquid and the contact angle between the liquid and the walls, and w and h denote the dimensions of the channel behind the transition and W and H the dimensions of the channel before the transition.
  • the microfluidic arrangement can Inlet reservoir, which is the inlet of the first channel upstream.
  • This inlet reservoir can then according to the Invention a smaller capillary force than the first channel in the area of the inlet.
  • the microfluidic arrangement can be an outlet reservoir have, which downstream of the outlet of the first channel is.
  • This outlet reservoir advantageously has a greater capillary force as the first channel in the area of the outlet.
  • the first channel of the microfluidic arrangement can according to the Invention be divided into sections between inlet and outlet, so that the first channel forms a first channel system.
  • the sections of the first channel system one of the Inlet to the outlet have increasing capillary force.
  • the second channels can be divided into sections be split so that the second channels a second channel system form, wherein the capillary force of these sections of the second Channel systems advantageous from the branch points to the Means for stopping increases.
  • the second channels may then follow the stopping means depending connect a third channel.
  • a microfluidic according to the invention Arrangement can also be designed so that to the second channels a common third channel connects.
  • a third channel for example, a reaction chamber formed be in which reagents are provided.
  • the metered Liquid which is contained in a second channel, in the third channel where the dosed liquid then reacts with the reagents in the reaction chamber and a desired product arises.
  • the capillary force of the third channels are larger than those of the second channels.
  • the stopping means capillary stops, Be microvalves or other suitable stopping means.
  • the microfluidic arrangement can follow the third Channels each have a second outlet, via which for example, the product of the microfluidic arrangement can be removed. It is also possible the microfluidic Arrangement and in particular the second and the third channel via vent this second outlet. About the second outlet can Furthermore, another reaction chamber or similar. with the invention Be connected arrangement.
  • individual or all Sections of the first channel system, the second channel systems and / or the third channel systems meandering, as cavities and / or be configured with an absorbent material. Further can the microfluidic arrangement in the range of Branching point be connected to a ventilation duct.
  • FIGS. 1 to 8 c for Microfluidic arrangements can be part of a larger Overall arrangement. You can, for example, with others Microfluidic arrangements for the same or for others Purposes on a sample carrier z.
  • a sample carrier z As plastic or silicon, be provided in particular a microtiter plate.
  • the principle of the invention is based on the simplified embodiment illustrated in FIG. 8a to 8c become.
  • the microfluidic arrangement according to FIGS. 8 to 8c has a first channel 2 with an inlet and an outlet 12.
  • a second channel 16 branches from the first channel 2 from.
  • This second channel 16 has at the Branch point 4 a greater capillary force than the first channel. 2
  • the second channel 16 has a precisely determined volume.
  • a Liquid, which via the inlet 11 into the first channel. 2 is introduced, due to acting in the first channel 2 Capillary forces transported to the branch point 4.
  • the second channel 16 contains at this moment a first exactly metered amount of liquid. Another part of the liquid, which is introduced at the inlet 11 in the first channel 2, is about the branch point 4 in the direction of the outlet 12th transported. Once at the inlet 11 no more liquid more enters the first channel 2 migrates a gas bubble in the first channel 2 a. This will reach the sooner or later Branch point 4.
  • the example shown in FIG. 1 for a microfluidic Arrangement has a first channel 2, a second channel 16 and a third channel 10.
  • both the first channel 2, the second channel 16 and the third channel 10 a plurality of sections on, which is why the channels 2, 16, 10 hereinafter as the first Channel system 2, second channel system 16 and third, respectively Channel system 10 are called.
  • the first one Embodiment an inlet reservoir 1, an outlet reservoir. 3 and a ventilation channel 5.
  • the ventilation channel 5 can for ventilation of the duct systems also a ventilation over the Inlet reservoir 1 carried out, which in a manner not shown for Filling with a liquid is connected to the environment.
  • the enumerated components of the first embodiment of a microfluidic arrangement are called cavities in a sample carrier intended.
  • the cavities are as recesses, trenches and / or Nute arranged in a surface of the sample holder.
  • This Surface of the sample carrier is then by means of a foil, a Cover or similar covered.
  • microfluidic devices can also be used by others in the field Common realizations are chosen as the introduction of Recesses and / or grooves in the surface of a Sample carrier.
  • the inlet reservoir 1 can from the outside via a not shown Opening to be filled with a liquid.
  • the first channel system 2 joins the first channel system 2, the a channel, an inlet 11 and an outlet 12, between which the channel extends.
  • the inlet reservoir 1 is connected via the inlet 11 to the first channel system 2.
  • On the outlet 12 of the first channel system 2 then closes the Outlet reservoir 3 at.
  • This outlet reservoir 3 is via a Venting duct connected to the environment. Instead, that can Outlet reservoir 3 via holes, channels or the like in the Cover to be vented.
  • the second channel system 16 branches from the channel of the first channel system 2 at one Branch point 4, the second channel system 16 from.
  • the form and the area of the cross section of the channels at the branching point 4th at the transition from the first channel system 2 to the second channel system 16, changes abruptly. In these places goes - in Transport direction seen - a flat and wide channel in one deep narrow channel above.
  • the second channel system 16 consists of three sections 6, 7, 8, namely a first section 6, which is designed as a groove and so forms a channel, a second section 7, which as Recess is executed, and a third section 8, as a groove is executed and thus forms a channel.
  • first section 6 at the branch point 4 with the first channel system 2 connected.
  • second section 7 closes the second section 7, which then turn the third Section 8 connects.
  • the third section 8 forming channel opens into a capillary stop 9.
  • the third channel system 10 which consists essentially of a recess which communicates via two channels on the one hand the capillary stop 9 and on the other hand with a second outlet 13th is connected, which opens into the environment.
  • the second channel system 6, 7, 8, and / or the third channel system 10 with be provided a venting or ventilation duct.
  • the individual channel systems of the embodiment have a different capillary force. So is the particular Capillary force of the second channel system 16 at the branch point 4 greater than the capillary force of the first channel system 2 at this Branch point 4. In addition, the capillary force of the first Channel system 2 greater than the capillary force of the inlet reservoir. 1 Conversely, on the other hand, the capillary force of the first channel system 2 less than or equal to the outlet reservoir 3.
  • the sections 6, 7, 8 a same Have capillary force.
  • the Capillary force of the third section 8 is greater than that of the second Section 7 and that of the second section 7 is greater than the first section 6.
  • the ventilation duct 5 advantageously has a Capillary force smaller than that of the first channel system 2.
  • the venting channel 14 advantageously has a capillary force, the smaller than that of the outlet reservoir 3.
  • the volume of the inlet reservoir 1 is less than or equal to Sum of the volumes of the outlet reservoir 3 and the second Channel system 16.
  • the inlet reservoir 1 is filled with a liquid, the following happens: due to the higher capillary force in the first Channel system 2 will include those contained in the inlet reservoir 1 Liquid transported in the second channel system 2, which is from the inlet 11 in the direction of the branch point 4 filled. As soon as the liquid has reached the branch point 4, the shares Liquid flow on. Due to the higher capillary force of the second Channel system 16 becomes part of the inlet 11 into the first Channel system 2 inflowing liquid in the second channel system 16 promoted. Another much smaller part, which in the second Channel system 2 inflowing liquid, is over the Branch point 4 addition in the second channel system 2 to the Outlet 12 transported.
  • the capillary forces are in this case to each other chosen that the inflowing into the second channel system 16 Fluid has completely filled this second channel system 16, before the inlet reservoir 1 and between the inlet 11 and the branch point 4 lying part of the first channel system. 2 completely emptied of liquid. Ie. at the moment, in the the liquid flowing into the second channel system 16 Capillary stop 9 has reached, is still at branch point 4 Liquid contained in the first channel system 2. This can do that predetermined volumes of this second channel system 16 completely be filled, resulting in this second channel system 16 a precisely adjusted volume of the liquid sets. But it is the same also conceivable, the first channel system 2 in the area of Branch point 4 contains no liquid if the second Channel system is completely filled.
  • Liquid quantities namely in the second channel system 6, 7, 8 contained metered amount of liquid A and in the Outlet reservoir 3 contained undosed liquid amount B are fluidically through the gas phase contained in the first channel system 2 separated from each other.
  • liquid contained can then after the effect of the Capillary stops 9 are transported into the third channel system 10. This is closer with reference to FIG. 7 for the second embodiment described.
  • the second embodiment shown in FIGS. 2 to 6 corresponds in many parts to that shown in FIG. 1 first Embodiment. Therefore, the same elements are the Microfluidic arrangements according to FIG. 1 or FIG. 2 to FIG. 6 with provided the same reference numerals.
  • the first embodiment and the second embodiment differ only in that that in the second embodiment, several, namely three second channel systems 16 and three third channel systems 10 are provided are.
  • the inlet reservoir 1 is significantly larger formed, wherein the volume of the inlet reservoir 1 also here less than or equal to the sums of the volumes of the second Channel systems 16 and the second outlet reservoir 3 is.
  • the three second channel systems 16 branch in the direction of Inlet 11 to the outlet 12 in a row from the channel of the first Channel system 2 at branch points 4 from. To the Branching points 4 close each of the first sections. 6 of the second channel system 16. These first sections 6 of the Kanalals then lead as already from the first Embodiment known, in a second section 7, which then in the third section 8 opens.
  • the third section 8 of the second Channel system 16 then ends at a respective Kapillarstopp 9. Behind The capillary stop 9 then closes in each case a third channel system 10, which opens into a respective second outlet 13.
  • the inlet reservoir 1 filled from the outside with a liquid passes this liquid at the inlet 11 in the first channel system 2 a. Due to the acting capillary forces, the liquid drifts into the Channel of the first channel system 2 in (Fig. 2) until they on the first branch point 4 arrived. At this point branches Liquid flow in the left in the illustration arranged second Channel system 16 a. This left second channel system 16 is completely filled with the liquid. Only then will the liquid become further in the channel of the first channel system 2 clearly propelled (Fig. 3). Once it's arranged in the middle Branched point 4 branches off the stream in the channel of the first channel system 2, a further liquid flow in the centrally located second channel system 16 (FIG. 4).
  • the right second Channel system 16 filled. Is this also complete with the liquid filled, the remaining in the channel of the first channel system 2 located liquid and optionally in the Inlet reservoir 1 remaining liquid due to the capillary forces transported in the outlet reservoir 3 (Fig. 5), wherein in the Outlet reservoir 3 displaced air via the vent passage 14th can escape.
  • the defined volumes of the second Channel systems 16, essentially filled one after the other.
  • the capillary force be adjusted so that the second channel systems 16, be filled approximately simultaneously. The remains in the first Channel system 2 flowing liquid is not in the Branch points to complete filling but is transported to the first outlet 13 without great delay. Once the liquid has reached the branching points 4 begins then filling the at the respective branching point 4th excluded second channel system 16.
  • the transport takes place this is advantageous due to the action of capillary forces, as it is but also a transport due to pressure - be it Overpressure, be it underpressure - possible.
  • the dosed liquids A then react in the reaction chambers with the arranged there Reagents to produce the desired product.
  • capillary stops 9 can be generated.
  • One of them is a simple one Cross-sectional enlargement of the channel section. At this point would be then reduces the capillary force, creating a liquid flow to the Standing comes. It is also known to be hydrophobic or rough Provide surfaces in the channels to stop the To reach liquid.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • General Physics & Mathematics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
EP04001179A 2003-01-23 2004-01-21 Configuration microfluidique pour le dosage des liquides Expired - Lifetime EP1440732B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10302721A DE10302721A1 (de) 2003-01-23 2003-01-23 Mikrofluidische Anordnung zum Dosieren von Flüssigkeiten
DE10302721 2003-01-23

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP09177533.8 Division-Into 2009-11-30

Publications (2)

Publication Number Publication Date
EP1440732A1 true EP1440732A1 (fr) 2004-07-28
EP1440732B1 EP1440732B1 (fr) 2010-07-07

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EP04001179A Expired - Lifetime EP1440732B1 (fr) 2003-01-23 2004-01-21 Configuration microfluidique pour le dosage des liquides

Country Status (7)

Country Link
US (1) US8084004B2 (fr)
EP (1) EP1440732B1 (fr)
JP (2) JP2004226412A (fr)
CN (1) CN100560208C (fr)
AT (1) ATE473051T1 (fr)
DE (2) DE10302721A1 (fr)
ES (1) ES2345771T3 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1685900A1 (fr) 2005-01-27 2006-08-02 Boehringer Ingelheim microParts GmbH Dispositif et procédé pour l'analyse d'un échantillon de liquide
EP1946830A1 (fr) * 2005-11-07 2008-07-23 Konica Minolta Medical & Graphic, Inc. Microreacteur et procede d alimentation en liquide l utilisant
US8486350B2 (en) 2008-05-09 2013-07-16 Konica Minolta Medical & Graphic, Inc. Microchip, microchip liquid supply system, and microchip liquid supply method
EP3951402A4 (fr) * 2019-04-02 2023-01-18 H.U. Group Research Institute G.K. Dispositif à canal d'écoulement et système d'inspection

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US20070014694A1 (en) * 2003-09-19 2007-01-18 Beard Nigel P High density plate filler
US20050232821A1 (en) * 2003-09-19 2005-10-20 Carrillo Albert L High density plate filler
US7998435B2 (en) 2003-09-19 2011-08-16 Life Technologies Corporation High density plate filler
US8277760B2 (en) 2003-09-19 2012-10-02 Applied Biosystems, Llc High density plate filler
US20050249641A1 (en) * 2004-04-08 2005-11-10 Boehringer Ingelheim Microparts Gmbh Microstructured platform and method for manipulating a liquid
DE102004063438A1 (de) * 2004-12-23 2006-07-06 Oktavia Backes Neuartige mikrofluidische Probenträger
JP4548174B2 (ja) * 2005-03-24 2010-09-22 コニカミノルタエムジー株式会社 検査用マイクロチップおよびそれを用いた検査装置
DE102005042601A1 (de) * 2005-04-09 2006-10-12 Boehringer Ingelheim Microparts Gmbh Vorrichtung und Verfahren zur Untersuchung einer Probenflüssigkeit
JP4837725B2 (ja) * 2005-04-09 2011-12-14 ベーリンガー インゲルハイム マイクロパーツ ゲゼルシャフト ミット ベシュレンクテル ハフツング サンプル液の検査装置及び方法
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DE502004011349D1 (de) 2010-08-19
ES2345771T3 (es) 2010-10-01
ATE473051T1 (de) 2010-07-15
US8084004B2 (en) 2011-12-27
CN100560208C (zh) 2009-11-18
EP1440732B1 (fr) 2010-07-07
JP2004226412A (ja) 2004-08-12
DE10302721A1 (de) 2004-08-05
JP2010019852A (ja) 2010-01-28
CN1524619A (zh) 2004-09-01
JP5039766B2 (ja) 2012-10-03

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