EP2688670A1 - Système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique - Google Patents

Système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique

Info

Publication number
EP2688670A1
EP2688670A1 EP12703472.6A EP12703472A EP2688670A1 EP 2688670 A1 EP2688670 A1 EP 2688670A1 EP 12703472 A EP12703472 A EP 12703472A EP 2688670 A1 EP2688670 A1 EP 2688670A1
Authority
EP
European Patent Office
Prior art keywords
channel
filter
microfluidic
filter chamber
valve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP12703472.6A
Other languages
German (de)
English (en)
Other versions
EP2688670B1 (fr
Inventor
Peter Rothacher
Jochen Rupp
Christian Dorrer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2688670A1 publication Critical patent/EP2688670A1/fr
Application granted granted Critical
Publication of EP2688670B1 publication Critical patent/EP2688670B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves

Definitions

  • the subject of the present invention is a microfluidic filter chamber with an adjustable venting channel and its use.
  • the invention also relates to a fluidic system for bubble-free filling a
  • Purpose may e.g. the accumulation of bacteria or the purification of DNA fragments. Depending on the application come as a filter fabric mats or
  • Particle fillings made of glass, silicates, oxides, polymers, etc. are used.
  • filters pressed into plastic tubes are commercially available, e.g. QIAquick Purification Kit from Qiagen®, such filters are known from DE10218554A1. These filters are manually filled by pipetting and then centrifuged.
  • LOC Lab-on-Chip
  • ⁇ Micro-Total-Analysis-System
  • the invention relates to a microfluidic filter chamber comprising a filter, a venting channel, an inlet channel and an outlet channel, wherein the filter between the inlet channel and outlet channel is inserted, and wherein the venting channel branches from the inlet channel and wherein the flow through the microfluidic filter chamber is adjustable by means of the venting channel ,
  • the microfluidic filter chamber can be filled bubble-free. There are no air bubbles trapped during filling.
  • the filter is flowed homogeneously. Liquid flows can be regulated precisely. The complete rinsing of the filter is guaranteed. There is no clogging of components or undesirable reactions. The mixing of liquids is facilitated. Foaming is prevented. The fluidic resistance of the system is kept constant. Reagents contained in the filter chamber can be exchanged in a controlled manner.
  • the microfluidic filter chamber comprises a valve for controllable passage through the vent passage. This serves to regulate the passage of liquid or gas.
  • a particular embodiment of the microfluidic filter chamber is characterized in that the venting duct either leads to the atmosphere or leads into a separate reservoir. This serves to dissipate the air.
  • Another particular embodiment of the microfluidic filter chamber is characterized in that the venting channel opens into the outlet channel downstream of the filter. This serves to dissipate the air.
  • the microfluidic filter chamber is characterized in that the inlet channel is widened before and / or after the filter.
  • a particularly preferred embodiment of the microfluidic filter chamber is characterized in that the inlet channel is widened in front of the filter in order to ensure a homogeneous flow against the filter
  • Embodiment of the microfluidic filter chamber is characterized in that the outlet channel is widened after the filter.
  • Embodiment relates to a fluidic system comprising a multi-layer structure of at least two layers and a microfluidic filter chamber.
  • the invention also relates to a fluidic system comprising a multilayer construction of at least two layers and a microfluidic filter chamber comprising a filter, a venting channel, an inlet channel and an outlet channel, wherein the filter is inserted into the inlet channel, and wherein before the filter from the inlet channel branches off the venting channel and wherein the flow through the vent passage can be regulated, for example by a valve.
  • one or more layers are structured planes.
  • a particular embodiment of the invention relates to a fluidic system comprising a cover, a first structured plane and a second structured plane, an inlet channel, an extension of the inlet channel, a filter, a valve and an outlet channel.
  • the fluidic system may further include a duct passage.
  • a further particular embodiment of the invention relates to a fluidic system, characterized in that an elastic film is located between the one and the other layer or an elastic film is located between the first structured plane and the second structured plane.
  • the fluidic system for bubble-free filling of a microfluidic filter chamber still comprises at least one further valve.
  • the invention further provides a method for the bubble-free filling of a microfluidic filter chamber comprising a filter and a
  • Venting channel wherein a liquid is pumped through the inlet channel to the filter, while the valve is open in the venting channel, and
  • the filter is filled capillary, and then the channel area in front of the filter, the venting channel and the channel area are filled after the filter, and then the valve is closed.
  • the invention also provides a method for filtering a liquid with a microfluidic filter chamber, wherein first the microfluidic
  • Venting channel is open, then the filter is filled capillary, then
  • the channel region is filled after the filter, and then the valve is closed, and then the liquid to be filtered flows through the inlet channel and then flows through the filter into the outlet channel.
  • the filter may be a fabric filter or a silica filter.
  • fabric mats or particle beds made of glass, silicates, oxides or polymers are used as filters.
  • all filters can be used which are suitable for fluidic systems, in particular for microfluidic systems.
  • the radius of the filter is adjusted to the size of the microfluidic filter chamber. It can be between 1 and 25 mm. Preferably, the radius of the filter is 2 to 5 mm, more preferably 3.5 mm.
  • Fluidic channels are channels through which the liquid in one
  • microfluidic system can flow.
  • the dimensions of the fluidic channels are adapted to the respective requirements.
  • To the fluidic Channels include the inlet channel, the outlet channel, the vent channel and the channel extension.
  • the fluidic channels include the
  • the fluidic channels have one
  • Diameter or a width of 0.05 to 2 mm preferably from 0.2 to 1 mm, particularly preferably 0.3 or 0.5 mm, and a depth of 0.05 to 1.5 mm, preferably from 0.2 to 1 mm, especially preferably 0.3 or 0.5 mm.
  • the venting channel is adjustable, i. the flow of liquid or gas through the vent channel can be regulated.
  • the venting channel can be regulated via a valve.
  • the valve can be opened, partially opened or closed.
  • Valves serve to regulate the fluid flows in a fluidic
  • Filter chamber and in the fluidic systems may act as a valve.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne une chambre de filtration microfluidique munie d'un canal d'évacuation d'air réglable, et son utilisation. La présente invention concerne également un système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique et de filtrage de liquides, ainsi qu'un procédé de remplissage sans bulles d'une chambre de filtration microfluidique et un procédé de filtrage de liquides.
EP12703472.6A 2011-03-23 2012-01-23 Système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique Active EP2688670B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011005932.6A DE102011005932B4 (de) 2011-03-23 2011-03-23 Fluidisches System zur blasenfreien Befüllung einer mikrofluidischen Filterkammer sowie Verfahren zur blasenfreien Befüllung und Verfahren zum Filtern einer Flüssigkeit mit einem solchen System
PCT/EP2012/050948 WO2012126647A1 (fr) 2011-03-23 2012-01-23 Système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique

Publications (2)

Publication Number Publication Date
EP2688670A1 true EP2688670A1 (fr) 2014-01-29
EP2688670B1 EP2688670B1 (fr) 2019-08-07

Family

ID=45581838

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12703472.6A Active EP2688670B1 (fr) 2011-03-23 2012-01-23 Système fluidique de remplissage sans bulles d'une chambre de filtration microfluidique

Country Status (4)

Country Link
EP (1) EP2688670B1 (fr)
DE (1) DE102011005932B4 (fr)
ES (1) ES2753534T3 (fr)
WO (1) WO2012126647A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102018220898B4 (de) 2018-12-04 2022-10-13 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Filterung eines Fluids
DE102021212645A1 (de) 2021-11-10 2023-05-11 Robert Bosch Gesellschaft mit beschränkter Haftung Vorrichtung und Verfahren zur Durchführung mikrofluidischer Prozessschritte
DE102022203627A1 (de) 2022-04-11 2023-10-12 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6416293B1 (en) * 1999-07-20 2002-07-09 Deka Products Limited Partnership Pumping cartridge including a bypass valve and method for directing flow in a pumping cartridge
US6867049B1 (en) * 2000-09-27 2005-03-15 Becton, Dickinson And Company Method for obtaining increased particle concentration for optical examination
US6811695B2 (en) 2001-06-07 2004-11-02 Nanostream, Inc. Microfluidic filter
EP1536875B1 (fr) * 2002-04-19 2011-11-23 3M Innovative Properties Company Cartouche filtrante encapsulee
DE10218554A1 (de) 2002-04-25 2003-11-06 Qiagen Gmbh Aufreinigung von Flüssigkeiten
DE10345818A1 (de) 2003-09-30 2005-04-28 Boehringer Ingelheim Micropart Verfahren und Vorrichtung zum Separieren und Abführen von Gasblasen aus Flüssigkeiten
US20070031283A1 (en) * 2005-06-23 2007-02-08 Davis Charles Q Assay cartridges and methods for point of care instruments
WO2007006049A2 (fr) 2005-07-06 2007-01-11 The Regents Of The University Of California Dispositifs, systemes et procedes pour isoler et separer des substances biologiques
US20090188856A1 (en) * 2007-10-20 2009-07-30 Robb Benson Externally Centering Filter Element or Cartridge and Housing and System Utilizing the Same
BRPI0915278A2 (pt) * 2008-11-13 2019-09-24 Koninl Philips Electronics Nv sistema microfluídico e método para preencher um canal capilar

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2012126647A1 *

Also Published As

Publication number Publication date
DE102011005932B4 (de) 2022-07-14
EP2688670B1 (fr) 2019-08-07
ES2753534T3 (es) 2020-04-13
DE102011005932A1 (de) 2012-09-27
WO2012126647A1 (fr) 2012-09-27

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