EP1179340A3 - Compositions for the introduction of polyanionic materials into cells - Google Patents

Compositions for the introduction of polyanionic materials into cells Download PDF

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Publication number
EP1179340A3
EP1179340A3 EP01127569A EP01127569A EP1179340A3 EP 1179340 A3 EP1179340 A3 EP 1179340A3 EP 01127569 A EP01127569 A EP 01127569A EP 01127569 A EP01127569 A EP 01127569A EP 1179340 A3 EP1179340 A3 EP 1179340A3
Authority
EP
European Patent Office
Prior art keywords
cells
compositions
introduction
polyanionic
polyanionic materials
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01127569A
Other languages
German (de)
French (fr)
Other versions
EP1179340A2 (en
Inventor
Steven Michial Ansell
Barbara Mui
Michael Hope
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inex Pharmaceuticals Corp
Original Assignee
Inex Pharmaceuticals Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/442,267 external-priority patent/US5753613A/en
Application filed by Inex Pharmaceuticals Corp filed Critical Inex Pharmaceuticals Corp
Publication of EP1179340A2 publication Critical patent/EP1179340A2/en
Publication of EP1179340A3 publication Critical patent/EP1179340A3/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids

Abstract

The present invention provides compositions and methods which are useful for the introduction of polyanionic materials into cells. The compositions are mixtures of cationic compounds and neutral lipids which are typically formulated as liposomes. The cationic compounds are quaternary ammonium compounds wherein the nitrogen has two attached long chain alkyl groups, at least one of which is unsaturated. The methods for transfecting cells involve (a) contacting the polyanionic materials with the compositions above to form a polyanionic material-liposome complex, and (b) contacting the complex with the cells to be transfected.
EP01127569A 1994-09-30 1995-09-29 Compositions for the introduction of polyanionic materials into cells Withdrawn EP1179340A3 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US31639994A 1994-09-30 1994-09-30
US316399 1994-09-30
US08/442,267 US5753613A (en) 1994-09-30 1995-05-16 Compositions for the introduction of polyanionic materials into cells
US442267 1995-05-16
EP95932591A EP0783296B1 (en) 1994-09-30 1995-09-29 Novel compositions for the introduction of polyanionic materials into cells

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP95932591A Division EP0783296B1 (en) 1994-09-30 1995-09-29 Novel compositions for the introduction of polyanionic materials into cells
EP95932591.1 Division 1996-05-10

Publications (2)

Publication Number Publication Date
EP1179340A2 EP1179340A2 (en) 2002-02-13
EP1179340A3 true EP1179340A3 (en) 2003-05-07

Family

ID=26980411

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01127569A Withdrawn EP1179340A3 (en) 1994-09-30 1995-09-29 Compositions for the introduction of polyanionic materials into cells

Country Status (5)

Country Link
US (1) US5785992A (en)
EP (1) EP1179340A3 (en)
AT (1) ATE219660T1 (en)
CA (1) CA2200952C (en)
DE (1) DE69527206T2 (en)

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Also Published As

Publication number Publication date
EP1179340A2 (en) 2002-02-13
CA2200952C (en) 2006-04-11
ATE219660T1 (en) 2002-07-15
DE69527206D1 (en) 2002-08-01
US5785992A (en) 1998-07-28
DE69527206T2 (en) 2003-02-27
CA2200952A1 (en) 1996-04-11

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