EP1036594A2 - Générateur multicanaux de gouttelettes - Google Patents

Générateur multicanaux de gouttelettes Download PDF

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Publication number
EP1036594A2
EP1036594A2 EP00104462A EP00104462A EP1036594A2 EP 1036594 A2 EP1036594 A2 EP 1036594A2 EP 00104462 A EP00104462 A EP 00104462A EP 00104462 A EP00104462 A EP 00104462A EP 1036594 A2 EP1036594 A2 EP 1036594A2
Authority
EP
European Patent Office
Prior art keywords
outlets
distributor
section
cross
drop generator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP00104462A
Other languages
German (de)
English (en)
Other versions
EP1036594B1 (fr
EP1036594A3 (fr
Inventor
Thomas Moore
Sebastian Pobering
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cybio Instruments GmbH
Original Assignee
Jenoptik Jena GmbH
Cybio Instruments GmbH
Jenoptik AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jenoptik Jena GmbH, Cybio Instruments GmbH, Jenoptik AG filed Critical Jenoptik Jena GmbH
Publication of EP1036594A2 publication Critical patent/EP1036594A2/fr
Publication of EP1036594A3 publication Critical patent/EP1036594A3/fr
Application granted granted Critical
Publication of EP1036594B1 publication Critical patent/EP1036594B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • Multi-channel drop generators or also dispensing devices serve in particular to fill cavities in microtitration plates with liquid. They usually consist of a pump and a distributor connected to it via a line.
  • the solutions known from the prior art pursue the goal of filling cavities with the smallest possible volume differences, that is to say that the same volume is dispensed at all outlets of the distributor at all times.
  • different constructive solutions for the distributor were developed.
  • the distributor is often constructed as a tube distributor. However, due to its functional principle, this prohibits an exactly even distribution. Outputs whose distance to the inlet of the distributor is shorter than more distant outputs are preferably flowed through. A variant of such a distributor is described in US Pat. No.
  • a dispensing device with a further variant of a distributor is known from EP 0 180 591 B1. This basically consists of a horizontal distributor pipe, from which at least two filling pipes first lead upwards and then after a deflection area into a drainage area downwards.
  • the filling pipes are connected to each other via a compensating pipe.
  • the line between the pump and the distributor pipe should be made of elastic material in order to somewhat compensate for the relatively hard pump shocks and to pull the liquid in the filler pipes back by about 1-2 mm after the completion of each pumping process, which should reliably prevent dripping.
  • this dispensing device is not suitable, since a slightly different liquid column height in the drainage areas of the filling pipes after the liquid has been withdrawn to different dispensing volumes at the individual outlets at the next one Dosing can lead.
  • the multi-channel drop generator is basically built and operated so that the levy on the one hand without contact and on the other hand via outlets that are crucial for the accuracy of volume delivery completely drained after each delivery become.
  • the dosing device 1 consists of a storage vessel 5 and a dosing device 6, which conveys the liquid from the storage vessel 5 via the connection 2 into a distributor 3 at the outlets of which there are outlets 4 which enable the droplets to be dispensed.
  • the amount of liquid must be precisely adjustable and conveyed with a high level of repeatability. Solenoid valves in connection with a pressure vessel or wobble piston pumps can be used advantageously.
  • the strong pulsation of the metering devices 1 generates a pressure pulse which is transmitted via the connection 2 to the distributor 3 and to the outlets 4 and which enables small drops to be dispensed there.
  • the connection is a preferably hose-like component of defined elasticity in order to reduce the steepness of the edges of the pressure pulses.
  • the distributor 3 is a planar channel system made of cascaded T-pieces with an inlet 12 and a plurality of outlets 13, as shown in FIGS. 1 and 2, which can be incorporated, for example, in plate material. Such a structure ensures an exactly uniform distribution of the liquid flowing through the outlets, with minimal dead volume, regardless of their fluidic properties. Furthermore, the distributor 3 effects an even distribution of the pressure pulses from the input to the outputs. Due to the large cross-section of the channels of the channel system compared to the outlets 4, the distributor 3 has only a negligible part in the dynamic properties of the multi-channel drop generator.
  • FIG. 3 This essentially represents a series resonant circuit with current excitation, which can be described with the differential equations from electrical engineering.
  • the metering device 1 is reproduced by the power source 7 with switch 8, the elastic connection by the capacitance 9. The distributor is neglected.
  • the outlets are shown as resistors 10 and inertias 11.
  • In a quasi-static view of the multi-channel drop generator if drops were slowly added by the metering device 1, 4 drops would form at the outlets, which tear off when the weight of the drop exceeds its surface tension. Thereafter, a so-called meniscus remains at the outlets 4, which leads to inaccuracies. Drops produced in this way have a volume of approx.
  • the metering device 1 conveys the volume to be dispensed at a high conveying speed via the connection 2 and the distributor 3 to the outlets 4.
  • liquid jets with the diameter of the outlets 4 form.
  • the inertia prevents droplets from forming. If the liquid flow is interrupted abruptly, the jet breaks off due to the high kinetic energy before a drop occurs. However, an indefinite amount of liquid remains at the outlets 4 and forms a meniscus. In order not to falsify the result of the following dispensing cycle, this liquid residue must be removed.
  • connection 2 which is designed as an elastic element and is capable of performing this function.
  • connection 2 At the beginning of a delivery cycle, the connection 2, as its cross section expands, absorbs part of the volume conveyed and in part releases it again in the further course. If the volume flow is interrupted, the inertia of the liquid columns in the outlets 4 causes the volume flow to continue for a short time. The flowing volume is supplied by the elasticity of the connection 2, whereby a negative pressure is created.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP00104462A 1999-03-08 2000-03-07 Générateur multicanaux de gouttelettes Expired - Lifetime EP1036594B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19911456A DE19911456A1 (de) 1999-03-08 1999-03-08 Mehrkanal-Tropfengenerator
DE19911456 1999-03-08

Publications (3)

Publication Number Publication Date
EP1036594A2 true EP1036594A2 (fr) 2000-09-20
EP1036594A3 EP1036594A3 (fr) 2001-01-24
EP1036594B1 EP1036594B1 (fr) 2005-06-15

Family

ID=7901017

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00104462A Expired - Lifetime EP1036594B1 (fr) 1999-03-08 2000-03-07 Générateur multicanaux de gouttelettes

Country Status (3)

Country Link
EP (1) EP1036594B1 (fr)
JP (1) JP2000258438A (fr)
DE (2) DE19911456A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1424130A2 (fr) * 2002-11-26 2004-06-02 CyBio AG Distributeur à canaux multiples avec calibrage automatique
EP1520618A2 (fr) * 2003-10-03 2005-04-06 Delta Biologicals S.r.l. Dispositif pour la distribution égale de petites quantités de fluides
US8932544B2 (en) 2006-06-28 2015-01-13 M2P-Labs Gmbh Microreactor array, device comprising a microreactor array, and method for using a microreactor array

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10000691A1 (de) * 2000-01-10 2001-07-26 Fraunhofer Ges Forschung Mikro-Düsen-System
DE10236029A1 (de) 2002-08-02 2004-02-19 Cybio Systems Gmbh Einrichtung zum Dispensieren und Beobachten der Lumineszenz von Einzelproben in Multiprobenanordnungen
CN104880353B (zh) * 2015-06-11 2017-12-29 中国矿业大学 一种多通道气流均匀润湿煤样装置
DE102016015700A1 (de) 2016-12-21 2018-06-21 Bayer Pharma Aktiengesellschaft Dosiervorrichtung
CN114225987B (zh) * 2021-11-15 2023-03-07 北京理工大学 一种可分别控制的双液滴生成装置

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3508878A (en) * 1965-12-29 1970-04-28 Yeda Res & Dev Containers for dispensing drop-size quantities of liquids
US4314259A (en) * 1980-06-16 1982-02-02 Arthur D. Little, Inc. Apparatus for providing an array of fine liquid droplets particularly suited for ink-jet printing
EP0180591A1 (fr) * 1984-04-10 1986-05-14 Prolic Ag Installation de distribution pour liquides.
US4730197A (en) * 1985-11-06 1988-03-08 Pitney Bowes Inc. Impulse ink jet system
US5334352A (en) * 1992-09-23 1994-08-02 Icn Biomedicals, Inc. Manifold construction
US5441204A (en) * 1993-06-10 1995-08-15 United Air Specialists, Inc. Electrostatic fluid distribution nozzle

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3508878A (en) * 1965-12-29 1970-04-28 Yeda Res & Dev Containers for dispensing drop-size quantities of liquids
US4314259A (en) * 1980-06-16 1982-02-02 Arthur D. Little, Inc. Apparatus for providing an array of fine liquid droplets particularly suited for ink-jet printing
EP0180591A1 (fr) * 1984-04-10 1986-05-14 Prolic Ag Installation de distribution pour liquides.
US4730197A (en) * 1985-11-06 1988-03-08 Pitney Bowes Inc. Impulse ink jet system
US5334352A (en) * 1992-09-23 1994-08-02 Icn Biomedicals, Inc. Manifold construction
US5441204A (en) * 1993-06-10 1995-08-15 United Air Specialists, Inc. Electrostatic fluid distribution nozzle

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1424130A2 (fr) * 2002-11-26 2004-06-02 CyBio AG Distributeur à canaux multiples avec calibrage automatique
EP1424130A3 (fr) * 2002-11-26 2005-09-07 CyBio AG Distributeur à canaux multiples avec calibrage automatique
US7379832B2 (en) 2002-11-26 2008-05-27 Cybio Ag Multi-channel metering apparatus with automatic calibration
US7553452B2 (en) 2002-11-26 2009-06-30 Cybio Ag Procedure for channel adjustment of a multi-channel metering apparatus
EP1520618A2 (fr) * 2003-10-03 2005-04-06 Delta Biologicals S.r.l. Dispositif pour la distribution égale de petites quantités de fluides
EP1520618A3 (fr) * 2003-10-03 2006-08-02 Delta Biologicals S.r.l. Dispositif pour la distribution égale de petites quantités de fluides
US8932544B2 (en) 2006-06-28 2015-01-13 M2P-Labs Gmbh Microreactor array, device comprising a microreactor array, and method for using a microreactor array

Also Published As

Publication number Publication date
DE50010538D1 (de) 2005-07-21
EP1036594B1 (fr) 2005-06-15
DE19911456A1 (de) 2000-09-14
JP2000258438A (ja) 2000-09-22
EP1036594A3 (fr) 2001-01-24

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