EP0904286A4 - Invasive cleavage of nucleic acids - Google Patents

Invasive cleavage of nucleic acids

Info

Publication number
EP0904286A4
EP0904286A4 EP97903931A EP97903931A EP0904286A4 EP 0904286 A4 EP0904286 A4 EP 0904286A4 EP 97903931 A EP97903931 A EP 97903931A EP 97903931 A EP97903931 A EP 97903931A EP 0904286 A4 EP0904286 A4 EP 0904286A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
specific
present
acid sequences
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97903931A
Other languages
German (de)
French (fr)
Other versions
EP0904286A1 (en
Inventor
Jeff G Hall
Victor I Lyamichev
James R Prudent
Mary Ann D Brow
Michael W Kaiser
Natasha Lyamichev
David Michael Olive
James E Dahlberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Third Wave Technologies Inc
Original Assignee
Third Wave Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/599,491 external-priority patent/US5846717A/en
Priority claimed from US08/756,386 external-priority patent/US5985557A/en
Priority claimed from US08/758,314 external-priority patent/US6090606A/en
Application filed by Third Wave Technologies Inc filed Critical Third Wave Technologies Inc
Priority to EP08020055A priority Critical patent/EP2058407B1/en
Priority to EP10179329A priority patent/EP2354252B1/en
Priority to EP05016686A priority patent/EP1634890B1/en
Publication of EP0904286A1 publication Critical patent/EP0904286A1/en
Publication of EP0904286A4 publication Critical patent/EP0904286A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charg
EP97903931A 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids Withdrawn EP0904286A4 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08020055A EP2058407B1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids
EP10179329A EP2354252B1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids
EP05016686A EP1634890B1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US599491 1990-10-17
US08/599,491 US5846717A (en) 1996-01-24 1996-01-24 Detection of nucleic acid sequences by invader-directed cleavage
US682853 1996-07-12
US08/682,853 US6001567A (en) 1996-01-24 1996-07-12 Detection of nucleic acid sequences by invader-directed cleavage
US756386 1996-11-29
US08/756,386 US5985557A (en) 1996-01-24 1996-11-29 Invasive cleavage of nucleic acids
US08/758,314 US6090606A (en) 1996-01-24 1996-12-02 Cleavage agents
US759038 1996-12-02
US08/759,038 US6090543A (en) 1996-01-24 1996-12-02 Cleavage of nucleic acids
US758314 1996-12-02
PCT/US1997/001072 WO1997027214A1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP05016686A Division EP1634890B1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids

Publications (2)

Publication Number Publication Date
EP0904286A1 EP0904286A1 (en) 1999-03-31
EP0904286A4 true EP0904286A4 (en) 2004-01-14

Family

ID=27541970

Family Applications (2)

Application Number Title Priority Date Filing Date
EP97903931A Withdrawn EP0904286A4 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids
EP05016686A Expired - Lifetime EP1634890B1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP05016686A Expired - Lifetime EP1634890B1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids

Country Status (8)

Country Link
EP (2) EP0904286A4 (en)
JP (2) JP3665648B2 (en)
AT (1) ATE414793T1 (en)
AU (1) AU731062B2 (en)
CA (1) CA2243353C (en)
DK (1) DK1634890T3 (en)
ES (1) ES2317113T3 (en)
WO (1) WO1997027214A1 (en)

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US7045289B2 (en) 1991-09-09 2006-05-16 Third Wave Technologies, Inc. Detection of RNA Sequences
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
AU781188B2 (en) * 1996-01-24 2005-05-12 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7527928B2 (en) 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US7432048B2 (en) 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
JP4362150B2 (en) * 1996-11-29 2009-11-11 サード ウェーブ テクノロジーズ，インコーポレーテッド FEN-1 endonuclease, mixture, and cleavage method
US6210880B1 (en) 1997-09-19 2001-04-03 Third Wave Technologies, Inc. Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
CA2289872C (en) 1997-05-05 2007-07-31 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
DK2216416T3 (en) 1997-05-30 2012-08-27 Trovagene Inc Methods for detecting nucleic acid sequences in urine
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
DE60111218T2 (en) * 2000-03-27 2006-03-23 Applera Corp., Foster City IMPROVED INVASION ASSAY
JP2004521606A (en) * 2000-05-24 2004-07-22 サード・ウェーブ・テクノロジーズ・インク RNA detection method
AU2001268513A1 (en) 2000-06-17 2002-01-02 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
WO2002002823A2 (en) 2000-07-03 2002-01-10 Applera Corporation Polynucleotide sequence assay
CA2428798C (en) * 2000-11-15 2013-09-03 Third Wave Technologies, Inc. Fen-1 endonucleases from archaeoglobus veneficus
EP1367136A4 (en) * 2001-02-15 2005-01-12 Takara Bio Inc Method of detecting nucleotide polymorphism
US7485443B2 (en) 2001-07-17 2009-02-03 Northwestern University Solid-phase reactions
US7303869B2 (en) 2001-07-17 2007-12-04 Northwestern University Solid-phase reactions
US20040014067A1 (en) 2001-10-12 2004-01-22 Third Wave Technologies, Inc. Amplification methods and compositions
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US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
AU2003297541A1 (en) 2002-12-18 2004-07-14 Third Wave Technologies, Inc. Detection of small nucleic acids
US7297780B2 (en) 2003-01-06 2007-11-20 Third Wave Technologies, Inc. Reactive functional groups for preparation of modified nucleic acid
US7700750B2 (en) 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
US20040161749A1 (en) 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
WO2005067643A2 (en) 2004-01-07 2005-07-28 Third Wave Technologies, Inc. Determination of hepatitis c virus genotype
US7462451B2 (en) 2004-04-26 2008-12-09 Third Wave Technologies, Inc. Compositions for modifying nucleic acids
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US7939257B2 (en) 2005-05-12 2011-05-10 Third Wave Technologies, Inc. Polymorphic GHSR nucleic acids and uses thereof
EP1931806B1 (en) 2005-10-07 2011-10-05 California Institute Of Technology Pkr activation via hybridization chain reaction
US7482127B2 (en) 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
US7674924B2 (en) 2006-05-22 2010-03-09 Third Wave Technologies, Inc. Compositions, probes, and conjugates and uses thereof
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US7759062B2 (en) 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
AU2007307171B2 (en) 2006-10-04 2012-01-19 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
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US8497364B2 (en) 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
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US20100068711A1 (en) 2008-07-18 2010-03-18 Xenomics, Inc. Methods of PCR-Based Detection of "Ultra Short" Nucleic Acid Sequences
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
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Also Published As

Publication number Publication date
CA2243353C (en) 2010-03-30
EP0904286A1 (en) 1999-03-31
EP1634890A1 (en) 2006-03-15
AU731062B2 (en) 2001-03-22
ES2317113T3 (en) 2009-04-16
WO1997027214A1 (en) 1997-07-31
DK1634890T3 (en) 2009-03-09
JP2002515737A (en) 2002-05-28
JP2005160481A (en) 2005-06-23
AU1836497A (en) 1997-08-20
ATE414793T1 (en) 2008-12-15
JP4295715B2 (en) 2009-07-15
EP1634890B1 (en) 2008-11-19
CA2243353A1 (en) 1997-07-31
JP3665648B2 (en) 2005-06-29

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