EP0894278B1 - Punktlichtquelle für ein laserscanmikroskop und verfahren zum einkoppeln von mindestens zwei lasern unterschiedlicher wellenlänge in ein laserscanmikroskop - Google Patents
Punktlichtquelle für ein laserscanmikroskop und verfahren zum einkoppeln von mindestens zwei lasern unterschiedlicher wellenlänge in ein laserscanmikroskop Download PDFInfo
- Publication number
- EP0894278B1 EP0894278B1 EP97922839A EP97922839A EP0894278B1 EP 0894278 B1 EP0894278 B1 EP 0894278B1 EP 97922839 A EP97922839 A EP 97922839A EP 97922839 A EP97922839 A EP 97922839A EP 0894278 B1 EP0894278 B1 EP 0894278B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- beam combiner
- lasers
- laser
- light source
- point light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/145—Beam splitting or combining systems operating by reflection only having sequential partially reflecting surfaces
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
Definitions
- the invention relates to a point light source for a laser scanning microscope, at least two lasers with different wavelengths can be coupled into the microscope are. Furthermore, the invention relates to a method for coupling at least two lasers of different wavelengths in a laser scanning microscope.
- Light sources of the type in question are used in laser scanning microscopes, especially needed in confocal microscopy.
- the main thing is as part of a multi-color application laser light in different wavelengths to couple the microscope. This has with special consideration of the Adjustability of the laser and taking into account the stability of the adjustment too respectively.
- the first alternative is a laser (ArKr) with multiple simultaneous wavelengths. It is inherent in the system, that all laser lines there - per se - are aligned with each other. The selection of the wavelengths as well as the power setting is supported by an AOTF (Acousto Optical Tunable Filter) reached. As a result, each laser line can be operated independently of the others continuously adjust their performance down to very low levels. The coupling takes place in each case through a single-mode fiber.
- the advantage of The above coupling option lies in a compact scanner, in one absolute adjustment stability and in a decoupling of the laser cooling caused vibration.
- An ArKr laser comprises - as already mentioned before - several laser lines simultaneously. Since these are generated in a resonator, this always causes one Instability of the individual lines, especially since only the total power in the laser through regulation is kept constant.
- the noise occurring here can vary depending on the setting of the total output are up to 10 percent "peak to peak" and thus reduced the image quality with regard to the signal / noise ratio. In contrast, achieve it Single line laser just 1 percent noise.
- the system is completely inoperative if the - singular - laser fails.
- the available laser lines always depend on the laser-active gas and its Mix off. Since this changes over the life of the laser, an impairment of all laser lines takes place over time. After all, only the laser lines of the gas mixture used are available, so that here always one There is a selection restriction.
- the invention is based on the object of a point light source for a laser scanning microscope to specify the benefits of the two aforementioned systems - Multiline lasers and several single-line lasers - uses and their disadvantages at least as far as possible excludes. Furthermore, a method for the use of such Point light source can be specified.
- the point light source according to the invention achieves the above object by Features of claim 1. Thereafter is a point light source for a laser scanning microscope characterized by at least two laser light sources that are in one Coupling in the beam combiner, and directly or indirectly from the beam combiner optical fiber leading to the microscope.
- the use of at least two independent laser light sources and thus at least two different ones Lasering brings the advantages previously discussed.
- the associated disadvantages can - likewise according to the invention - be switched off in that even before the Coupling into the laser scanning microscope takes place a beam combination, so that from there aligned laser beams or laser lines with several wavelengths - in the sense of a multiline laser - are available, as is the case with one Laser with several simultaneously occurring wavelengths is the case.
- the combined laser lines are simultaneously operated via a Optical fiber is guided to the microscope and there - like a laser with several simultaneously occurring wavelengths - coupled. Hence all laser lines are automatically adjusted to each other from the point of beam union in the fiber. An adjustment or setting therefore takes place exclusively in the beam combiner, which turns out to be compact component can be realized. A special adjustment to the coupling of the In any case, laser scanning microscope is not required.
- one of the lasers couples directly into the beam combiner.
- this laser is directly assigned to the beam combiner.
- At least one another laser is coupled into the beam combiner via an optical fiber, whereby it can be a single-mode fiber, in addition to the directly in
- the laser that couples the beam combiner can be specific and very special
- Laser lines can be used couple different wavelengths, so that a multi-color application results.
- the beam combiner can be a conventional one from the prior art Technically known component or a corresponding arrangement. So the beam combiner could be different as a dichroic beam combiner with divider mirrors Transmission / reflection characteristics. Any Arrangement for beam union can be used in any case, a monolithic Arrangement for reasons of stability, precision, simpler manufacture, but is also preferred for reasons of a smaller space requirement. A Interchangeability of the beam combiner against geometrically analog, but physically other "trimmed" facilities for beam unification can be realized.
- the beam combiner is arranged in a housing in a very particularly advantageous manner.
- the laser that is directly coupled into the beam combiner may be fixed or coupled to the housing via a special adapter head into the beam combiner directly on the housing via the adapter head.
- the others Lasers each couple via an optical fiber into the housing and thus into the beam combiner arranged therein and its beam path.
- the housing is preferably made of metal.
- a production from plastic is particularly advantageous if the housing is manufactured by injection molding leaves.
- the beam combiner is used to select and individually adjust the line output an AOTF (Acousto Optical Tunable Filter) is connected in a further advantageous manner.
- This AOTF is also inside to obtain a compact module arranged of the housing. With the help of the AOTF, among other things, any Compensate coupling losses.
- An AOM Acoustic Optical Modulator
- An AOM Acoustic Optical Modulator
- the arrangement within the housing lends itself, especially since it does a compact module can be realized overall.
- the optical fiber leading from the beam combiner to the microscope i.e. the fiber coupler is designed as a single-mode fiber. This ensures that minor misalignments result only from low coupling losses into the fiber impact. As already mentioned, these low coupling losses can be reduced and related changes in line performance ratios Compensate for using an AOTF.
- the inventive method for coupling at least two different lasers Wavelength in a laser scanning microscope solves the above Object by the features of claim 10. Thereafter, such a method characterized in that the lasers together as a singular point light source with simultaneous wavelengths or laser lines together as a singular point light source be coupled into the microscope.
- one of the lasers could be immediate and at least one other laser via an optical fiber into the encapsulated component - Beam combiner - to be coupled in.
- AOTF Acoustic Optical Tunable Filter
- the module shown in the single figure can be used as a point light source define for a laser scanning microscope or for a confocal microscope, whereby here a total of four lasers 1, 2, 3, 4 are provided.
- the lasers 1, 2, 3, 4 have different ones Wavelengths on.
- a beam combiner 5 into which the lasers 1, 2, 3, 4 are connected couple their laser beams.
- the "optical coupling" of all lasers 1, 2, 3, 4 a downstream AOTF (Acousto Optical Tunable Filter) 11 takes place exclusively over the lower divider level of the beam combiner 5.
- AOTF Acoustic Optical Tunable Filter
- Leading from the beam combiner 5 a common beam path through the AOTF 11 and an AOM (Acousto Optical Modulator) 13 via an optical fiber 6 to what is only indicated in the figure Laser scanning microscope 7.
- the single figure shows particularly clearly that one of the lasers, namely the one with the Reference numerals 4 marked lasers, directly coupled into the beam combiner 5.
- the other lasers 1, 2 and 3 each couple via an optical fiber 8 in the beam combiner 5.
- the beam combiner 5 is arranged in a housing 9. Accordingly, it is immediate lasers 4 coupling into the beam combiner 5 directly with the housing 9 connected.
- a corresponding adapter head is not shown here for the sake of simplicity.
- the other lasers 1, 2 and 3 lead via the optical fibers 8 through the housing wall through or through corresponding connections 10 into the interior of the housing, where coupling into the beam combiner 5 takes place.
- the beam combiner 5 is inside the housing 9 is followed by an AOTF (Acousto Optical Tunable Filter) 11, whereby this AOTF 11 is used for the selection and individual adjustment of the line output.
- the AOTF 11 is in turn followed by an AOM (Acousto Optical Modulator) 13, which is used for beam modulation and fast beam shutdown. From there guides the optical fiber 6, which is designed as a single-mode fiber, over a corresponding one Connection 12 to the laser scanning microscope 7, to there the laser beam with several Coupling wavelengths or several laser lines in the sense of a light point source.
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Microscoopes, Condenser (AREA)
Description
die einzige Figur eine schematische Darstellung einer erfindungsgemäßen Punktlichtquelle mit insgesamt vier Lasern.
- 1
- Laser
- 2
- Laser
- 3
- Laser
- 4
- Laser
- 5
- Strahlvereiniger
- 6
- Lichtleitfaser (vom Strahlvereiniger zum
Laserscanmikroskop) - 7
- Laserscanmikroskop, Mikroskop
- 8
- Lichtleitfaser (vom Laser zum Strahlvereiniger)
- 9
- Gehäuse
- 10
- Anschluß (in das Gehäuse)
- 11
- AOTF
- 12
- Anschluß (zum Laserscanmikroskop)
- 13
- AOM
Claims (14)
- Punktlichtquette mit mindestens zwei Lasern (1,2,3,4), unterschiedlicher Wellenlänge, einem mit den Lasern (1,2,3,4) optisch verbundenen Strahlvereiniger (5), der die Laserstrahlen der mindestens zwei Laser zusammenführt, wobei einer der Laser (4) unmittelbar in den Strahlvereiniger (5) einkoppelt, und mit einer nach dem Strahlvereiniger (5) vorgesehenen ersten Lichtleitfaser (6), die die zusammengeführten Laserstrahlen transportiert, dadurch gekennzeichnet, dass die Punktlichtquelle ein Laserscanmikroskop (7) umfasst zu dem die erste Lichtleitfaser (6) führt, dass mindestens ein zweiter der Laser mittels einer weiteren Lichtleitfaser (8) in den Strahlvereiniger (5) einkoppelt, und dass die Laserstrahlen der mindestens zwei Laser auf die erste Lichtleiterfaser (6) justierbar sind.
- Punktlichtquelle nach Anspruch 1, dadurch gekennzeichnet, dass der Strahlvereiniger (5) als dichroitischer Strahlvereiniger ausgeführt ist.
- Punktlichtquelle nach Anspruch 1, dadurch gekennzeichnet, dass der Strahlvereiniger (5) als monolithische Einheit ausgeführt ist.
- Punktlichtquelle nach einem der Ansprüche 1 bis 3, dadurch gekennzeichnet, dass der Strahlvereiniger (5) in einem Gehäuse (9) angeordnet ist und dass der unmittelbar in den Strahlvereiniger (5) einkoppelnde Laser (4) über einen Adapterkopf am Gehäuse (9) festgelegt ist.
- Punktlichtquelle nach Anspruch 4, dadurch gekennzeichnet, dass das Gehäuse (9) aus Metall hergestellt ist.
- Punktlichtquelle nach Anspruch 4, dadurch gekennzeichnet, dass das Gehäuse (9) aus Kunststoff hergestellt ist.
- Punktlichtquelle nach einem der Ansprüche 4 bis 6, dadurch gekennzeichnet, dass dem Strahlvereiniger (5) vorzugsweise innerhalb des Gehäuses (9) ein AOTF, Acousto Optical Tunable Filter, (11) zur Auswahl und individuellen Einstellung der Linienleistung nachgeschaltet ist.
- Punktlichtquelle nach einem der Ansprüche 4 bis 7, dadurch gekennzeichnet, dass dem Strahlvereiniger (5) vorzugsweise innerhalb des Gehäuses (9) ein AOM, Acousto Optical Modulator, (13) zur Strahlmodulation und schnellen Strahlabschaltung nachgeschaltet ist.
- Punktlichtquelle nach einem der Ansprüche 1 bis 8, dadurch gekennzeichnet, dass die vom Strahlvereiniger (5) zum Mikroskop (7) führende Lichtleitfaser (6) als Single-Mode-Faser ausgeführt ist.
- Verfahren zum Einkoppeln von mindestens zwei Lasern (1 und/oder 2 und/oder 3; 4) unterschiedlicher Wellenlänge in ein Laserscanmikroskop (7), gekennzeichnet durch die folgenden Schritte:Zusammenführen von mindestens zwei Lasern mit einem Strahlvereiniger, wobei einer der Laser (4) unmittelbar in den Strahlvereiniger (5) einkoppelt wirdVorsehen einer ersten Lichtleitfaser (6) nach dem Strahlvereiniger wobei die erste Lichtleitfaser (6) die zusammengeführten Laserstrahlen transportiert,Einkoppeln der mindestens zwei zusammengeführten Laser als gemeinsame, singuläre Punktlichtquelle in das Mikroskop (7);Einkoppeln des mindestens zweiten Lasers mittels einer weiteren Lichtleitfaser (8) in den Strahlvereiniger (5) einkoppelt, undJustieren der Laserstrahlen der mindestens zwei Laser auf die erste Lichtleifaser (6).
- Verfahren nach Anspruch 10, dadurch gekennzeichnet, dass der die zwei Laser zusammenführende Strahlvereiniger in einem Gehäuse (9) untergebracht ist.
- Verfahren nach einem der Ansprüche 10 oder 11, dadurch gekennzeichnet, dass vor der Einkopplung in das Mikroskop (7) in einem gekapselten Bauteil eine Strahlvereinigung stattfindet und dass von dort aus über die vorzugsweise als Single-Mode-Faser ausgeführte erste Lichtleitfaser (6) in das Mikroskop (7) eingekoppelt wird.
- Verfahren nach Anspruch 12, dadurch gekennzeichnet, dass einer der Laser (4) unmittelbar und mindestens ein weiterer Laser (1 und/oder 2 und/oder 3) über die weitere Lichtleitfaser (8) in das gekapselte Bauteil einkoppelt.
- Verfahren nach einem der Ansprüche 10 bis 13, dadurch gekennzeichnet, dass vor der Einkopplung in das Mikroskop (7) eine Auswahl und individuelle Einstellung der Linienleistung und/oder der Wellenlänge mittels eines AOTF, Acousto Optical Tunable Filter, (11) erfolgt.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19614929 | 1996-04-16 | ||
DE19614929 | 1996-04-16 | ||
DE19633185 | 1996-08-17 | ||
DE19633185A DE19633185C2 (de) | 1996-04-16 | 1996-08-17 | Mehrfarbige Punktlichtquelle für ein Laserscanmikroskop |
PCT/DE1997/000762 WO1997039375A1 (de) | 1996-04-16 | 1997-04-16 | Punktlichtquelle für ein laserscanmikroskop und verfahren zum einkoppeln von mindestens zwei lasern unterschiedlicher wellenlänge in ein laserscanmikroskop |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0894278A1 EP0894278A1 (de) | 1999-02-03 |
EP0894278B1 true EP0894278B1 (de) | 2004-11-03 |
Family
ID=26024773
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP97922839A Expired - Lifetime EP0894278B1 (de) | 1996-04-16 | 1997-04-16 | Punktlichtquelle für ein laserscanmikroskop und verfahren zum einkoppeln von mindestens zwei lasern unterschiedlicher wellenlänge in ein laserscanmikroskop |
Country Status (4)
Country | Link |
---|---|
US (1) | US6222961B1 (de) |
EP (1) | EP0894278B1 (de) |
JP (1) | JP4194118B2 (de) |
WO (1) | WO1997039375A1 (de) |
Families Citing this family (26)
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US20050111089A1 (en) * | 1994-07-15 | 2005-05-26 | Baer Stephen C. | Superresolving microscopy apparatus |
US6631226B1 (en) | 1997-01-27 | 2003-10-07 | Carl Zeiss Jena Gmbh | Laser scanning microscope |
EP1055144B1 (de) * | 1998-02-19 | 2015-01-14 | Leica Microsystems CMS GmbH | Optische anordnung mit spektral selektivem element |
DE19829981C2 (de) * | 1998-07-04 | 2002-10-17 | Zeiss Carl Jena Gmbh | Verfahren und Anordnung zur konfokalen Mikroskopie |
DE19936573A1 (de) | 1998-12-22 | 2001-02-08 | Zeiss Carl Jena Gmbh | Anordnung zur Separierung von Anregungs- und Emissionslicht in einem Mikroskop |
JP4324644B2 (ja) * | 1999-09-29 | 2009-09-02 | 株式会社オーク製作所 | 多波長レーザー光学系 |
EP1284296B1 (de) * | 2000-04-27 | 2006-10-11 | Consejo Superior De Investigaciones Cientificas | Methode zum nachweis von minderheitsgenomen in viralen quasi-spezies unter verwendung von dna-mikrochips |
DE10105391B4 (de) * | 2001-02-06 | 2004-11-25 | Leica Microsystems Heidelberg Gmbh | Scanmikroskop und Modul für ein Scanmikroskop |
DE10337558A1 (de) * | 2003-08-14 | 2005-03-10 | Leica Microsystems | Optische Vorrichtung und Mikroskop mit einer optischen Vorrichtung |
US7280570B2 (en) * | 2003-12-15 | 2007-10-09 | Leica Microsystems | Device for generating a light beam including multiple wavelengths |
EP1695132A1 (de) * | 2003-12-15 | 2006-08-30 | Leica Microsystems CMS GmbH | Vorrichtung zur bereitstellung eines laserlichtstrahls |
US20050201441A1 (en) * | 2003-12-15 | 2005-09-15 | Leica Microsystems Heidelberg Gmbh | Device for generating a laser light beam |
DE10361176A1 (de) * | 2003-12-15 | 2005-07-14 | Leica Microsystems Heidelberg Gmbh | Vorrichtung zur Erzeugung eines mehrere Wellenlängen umfassenden Lichtstrahls |
GB0519761D0 (en) | 2005-09-28 | 2005-11-09 | Point Source Ltd | Laser systems |
DE102006034908B4 (de) * | 2006-07-28 | 2023-01-26 | Carl Zeiss Microscopy Gmbh | Laser-Scanning-Mikroskop |
DE102007009820A1 (de) * | 2007-02-28 | 2008-09-04 | Osram Opto Semiconductors Gmbh | Optische Anordnung und optisches Verfahren |
DE102007047183A1 (de) * | 2007-10-02 | 2009-04-09 | Carl Zeiss Microimaging Gmbh | Spiegeltreppe zur Vereinigung mehrerer Lichtquellen und Laser-Scanning-Mikroskop |
US10114213B2 (en) | 2008-04-04 | 2018-10-30 | Cvi Laser, Llc | Laser systems and optical devices for manipulating laser beams |
US7903706B2 (en) * | 2008-04-04 | 2011-03-08 | O'shaughnessy John | Compact, thermally stable multi-laser engine |
US9413130B2 (en) | 2012-12-12 | 2016-08-09 | Cvi Laser, Llc | Optical systems |
US8975572B2 (en) | 2008-04-04 | 2015-03-10 | Cvi Laser, Llc | Compact, thermally stable fiber-optic array mountable to flow cell |
JP2009080502A (ja) * | 2008-12-05 | 2009-04-16 | Carl Zeiss Jena Gmbh | 走査ユニット付き顕微鏡における放射を走査ヘッドに結合するための配置およびその操作方法 |
US8998468B2 (en) * | 2012-03-16 | 2015-04-07 | Lumencor, Inc. | Solid state light source with hybrid optical and electrical intensity control |
US11506877B2 (en) | 2016-11-10 | 2022-11-22 | The Trustees Of Columbia University In The City Of New York | Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees |
US10584851B2 (en) * | 2018-04-17 | 2020-03-10 | John Joseph Tedesco | Modular multiwavelength light source |
US11378808B2 (en) | 2018-07-18 | 2022-07-05 | Idex Health & Science Llc | Laser systems and optical devices for laser beam shaping |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5147349A (en) * | 1988-10-07 | 1992-09-15 | Spectra-Physics, Inc. | Diode laser device for photocoagulation of the retina |
EP0666487A2 (de) | 1989-09-22 | 1995-08-09 | Fuji Photo Film Co., Ltd. | Rastermikroskop und Abtastmechanismus für dasselbe |
JP2561160B2 (ja) * | 1989-11-06 | 1996-12-04 | 富士写真フイルム株式会社 | 走査型顕微鏡 |
US5106192A (en) * | 1990-03-16 | 1992-04-21 | Eastman, Inc. | Polarization insensitive absolute interferometeric method and apparatus for measuring position angular bearing and optical paths |
US5410371A (en) * | 1993-06-07 | 1995-04-25 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Display system employing acoustro-optic tunable filter |
DE4324681C2 (de) | 1993-07-22 | 1997-09-04 | Hell Stefan | Verfahren zur optischen Anregung eines Energiezustands einer Probe in einem Probenpunkt und Vorrichtung zur Durchführung des Verfahrens |
DE4446185C2 (de) | 1994-08-25 | 1997-03-27 | Leica Lasertechnik | Vorrichtung zum Einkoppeln eines UV-Laserstrahls in ein konfokales Laser-Scanmikroskop |
-
1997
- 1997-04-16 US US09/147,131 patent/US6222961B1/en not_active Expired - Lifetime
- 1997-04-16 WO PCT/DE1997/000762 patent/WO1997039375A1/de active IP Right Grant
- 1997-04-16 EP EP97922839A patent/EP0894278B1/de not_active Expired - Lifetime
- 1997-04-16 JP JP53665097A patent/JP4194118B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0894278A1 (de) | 1999-02-03 |
JP2000508438A (ja) | 2000-07-04 |
JP4194118B2 (ja) | 2008-12-10 |
US6222961B1 (en) | 2001-04-24 |
WO1997039375A1 (de) | 1997-10-23 |
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