EP0655888A4 - Production of human hemoglobin in transgenic pigs. - Google Patents

Production of human hemoglobin in transgenic pigs.

Info

Publication number
EP0655888A4
EP0655888A4 EP93915317A EP93915317A EP0655888A4 EP 0655888 A4 EP0655888 A4 EP 0655888A4 EP 93915317 A EP93915317 A EP 93915317A EP 93915317 A EP93915317 A EP 93915317A EP 0655888 A4 EP0655888 A4 EP 0655888A4
Authority
EP
European Patent Office
Prior art keywords
human hemoglobin
production
transgenic pigs
pigs
transgenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP93915317A
Other languages
German (de)
French (fr)
Other versions
EP0655888A1 (en
Inventor
John S Logan
Steven H Holtzman
J Kevin O'donnell
Stephen H Pilder
Carl A Pinkert
Mark E Swanson
Hilary Keller
Ajay Sharma
Cynthia T Parsons
Ramesh Kumar
Steven P White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNX Corp
Original Assignee
DNX CORP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DNX CORP filed Critical DNX CORP
Publication of EP0655888A1 publication Critical patent/EP0655888A1/en
Publication of EP0655888A4 publication Critical patent/EP0655888A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to the use of transgenic pigs for the production of human hemoglobin in which, in certain embodiments, the pig beta globin promoter is used to facilitate the expression of human hemoglobin. The transgenic pigs of the invention may be used as an efficient and economical source of cell-free human hemoglobin that may be used for transfusions and other medical applications in humans.
EP93915317A 1992-06-12 1993-06-11 Production of human hemoglobin in transgenic pigs. Withdrawn EP0655888A4 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US89764892A 1992-06-12 1992-06-12
US897648 1992-06-12
US98789092A 1992-12-08 1992-12-08
US987890 1992-12-08
US3089793A 1993-03-15 1993-03-15
US30897 1993-03-15
PCT/US1993/005629 WO1993025071A1 (en) 1992-06-12 1993-06-11 Production of human hemoglobin in transgenic pigs

Publications (2)

Publication Number Publication Date
EP0655888A1 EP0655888A1 (en) 1995-06-07
EP0655888A4 true EP0655888A4 (en) 1997-06-11

Family

ID=27363743

Family Applications (1)

Application Number Title Priority Date Filing Date
EP93915317A Withdrawn EP0655888A4 (en) 1992-06-12 1993-06-11 Production of human hemoglobin in transgenic pigs.

Country Status (7)

Country Link
EP (1) EP0655888A4 (en)
JP (1) JPH07507921A (en)
AU (1) AU687743B2 (en)
CA (1) CA2137911A1 (en)
FI (1) FI945829A (en)
NZ (1) NZ253642A (en)
WO (1) WO1993025071A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922854A (en) * 1991-06-14 1999-07-13 Kumar; Ramesh Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids
US5821351A (en) * 1994-06-10 1998-10-13 Dnx Biotherapeutics Production of hemoglobin having a delta-like globin
EP0811072A1 (en) 1995-02-25 1997-12-10 Imperial Cancer Research Technology Limited Transgenic animals as model of psoriasis
US6022738A (en) * 1995-03-03 2000-02-08 Mount Sinai School Of Medicine Of The City University Of New York Vectors for expression of globin genes
US5741894A (en) * 1995-09-22 1998-04-21 Baxter International, Inc. Preparation of pharmaceutical grade hemoglobins by heat treatment in partially oxygenated form
US5861483A (en) 1996-04-03 1999-01-19 Pro-Neuron, Inc. Inhibitor of stem cell proliferation and uses thereof
JP4360915B2 (en) 2002-01-07 2009-11-11 ユーロスクリーン・ソシエテ・アノニム G protein-coupled receptor GPR43 ligand and use thereof
US20050227251A1 (en) 2003-10-23 2005-10-13 Robert Darnell Method of purifying RNA binding protein-RNA complexes
CN101189023B (en) 2005-03-31 2013-01-30 通用医疗公司 Monitoring and modulating hgf/hgfr activity
CA2665080C (en) 2005-10-01 2015-11-24 Charles Stout Regulatable fusion promoters
CN101886075B (en) * 2010-07-02 2011-12-21 东北农业大学 Porcine ROSA26 promoter and application thereof
GB201417042D0 (en) * 2014-09-29 2014-11-12 Fkd Therapies Oy Method
CA3090138A1 (en) 2018-02-02 2019-08-08 Bloodworks Antibody screens using transgenic antigen(s)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009179A1 (en) * 1987-05-16 1988-12-01 Somatogenetics International, Inc. Blood substitutes
WO1992022646A1 (en) * 1991-06-14 1992-12-23 Dnx Corp. Production of human hemoglobin in transgenic pigs

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6505690A (en) * 1989-09-26 1991-04-28 Richard R Behringer Erythroid-specific gene expression system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009179A1 (en) * 1987-05-16 1988-12-01 Somatogenetics International, Inc. Blood substitutes
WO1992022646A1 (en) * 1991-06-14 1992-12-23 Dnx Corp. Production of human hemoglobin in transgenic pigs

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BIEBER, F.A. & BRAUNITZER, G.: "Zur prä- und perinatalen Sauerstoffübertragung der Säuger : Die embryonalen Hämoglobinevom Hausschwein (Sus scrofa domestica)", HOPPE-SEYLER'S ZEITSCRIFT FÜR PHYSIOLOGISCHE CHEMIE, vol. 365, no. 3, March 1984 (1984-03-01), pages 321 - 334, XP000647018 *
HOFFMAN, S.J. ET AL.: "Expression of fully functional tetrameric human hemoglobin in Escherichia coli", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 87, no. 21, November 1990 (1990-11-01), WASHINGTON US, pages 8521 - 8525, XP002020074 *
International symposium on the role of Biotechnologies in preventive oncology, San Remo, Italy, June 11-12 1992 *
LOOKER, D. ET AL.: "A human recombinant haemoglobin designed for use as a blood substitute", NATURE, vol. 356, no. 6366, 19 March 1992 (1992-03-19), LONDON GB, pages 258 - 260, XP002020075 *
O'DONNELL, J.K. ET AL.: "Production of human hemoglobin in transgenic swine: an approach to a blood substitute", CANCER DETECTION AND PREVENTION, vol. 17, no. 2, 1993, pages 307 - 312, XP000606893 *
See also references of WO9325071A1 *

Also Published As

Publication number Publication date
AU4534393A (en) 1994-01-04
FI945829A0 (en) 1994-12-12
JPH07507921A (en) 1995-09-07
EP0655888A1 (en) 1995-06-07
CA2137911A1 (en) 1993-12-23
FI945829A (en) 1995-02-10
NZ253642A (en) 1996-10-28
AU687743B2 (en) 1998-03-05
WO1993025071A1 (en) 1993-12-23

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 19950109

AK Designated contracting states

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RHK1 Main classification (correction)

Ipc: C12N 15/12

A4 Supplementary search report drawn up and despatched

Effective date: 19970424

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RIN1 Information on inventor provided before grant (corrected)

Inventor name: SHARMA, AJAY

Inventor name: KELLER, HILARY

Inventor name: SWANSON, MARK E.

Inventor name: PINKERT, CARL A.

Inventor name: PILDER, STEPHEN H.

Inventor name: O'DONNELL, J.KEVIN

Inventor name: HOLTZMAN, STEVEN H.

Inventor name: LOGAN, JOHN S.

17Q First examination report despatched

Effective date: 19990914

STAA Information on the status of an ep patent application or granted ep patent

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18D Application deemed to be withdrawn

Effective date: 20011017